OPTO-EDU A33.1502 User manual

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To ensure the safety and obtain satisfactory performance, please study this

instruction manual thoroughly before start to use your instrument.

A33.1502

9.7" LCD Digital Microscope, 5.0M, Android Pad

Instruction Manual

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I. Introduction

Thank you for choosing Opto-Edu A33.1502 Biological Microscope. To ensure safety and obtain optimum performance

and familiarize yourself with the use of this microscope, we recommend that you read this manual thoroughly before

operating the microscope. Retain this instruction manual in an easily accessible place near the microscope for further

reference.

II.Caution

Attention

A33.1502 Biological Microscope can only be used inside the room. Place microscope on a stable plane and keep it in

balance. Power line must be retained in an easily accessible place to prevent any unnecessary accident.

Working Environment

As microscope is a precision instrument, improper using will make it unworkable or reduce its precision.

1. Do not expose the microscope in the sun directly

2. Temperature range is +10℃- +40℃，and the max. humidity is 85%

3. Avoid high temperature and humidification. Otherwise there will be fog or mold on the lens.

4. Avoid violent vibration as the vibration will reduce the images quality.

5. Do not place microscope in moist room to avoid short circuit. Please turn off the power supply as soon as water drop

in the microscope. If there are other things unsafe come into the microscope which may cause short circuit too, please

stop using and contact with manufacturer

Non-designated Application

1. Improper operation & usage may

cause damage to both instrument &

users. Please read instruction

manual carefully before using the

instrument.

2. Pay attention to the voltage. Do not

use other kinds of power line unless

there is specific description. Do not

take the microscope apart.

Instrument & accessories

described in this manual have

passed the safety & potential

risk test.

III. Structure

See Picture

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IV. Technical Parameter

A33.1502 9.7" LCD Digital Biological Microscope

Head

Trinocular Head, 30°Inclined, Interpupillary Distance 48-75mm, Both Eyepiece Tube

Diopter Adjustable

Eyepiece

WF10x/20mm, Diopter Adjustable, Dia. 30mm

Objective

High Contrast Chromatic Free

4x, 10x, 40x(S), 100x(Oil, S)

Focus Knob

Coaxial Coarse & Fine Focusing, Fine Division 0.002mm, Coarse Stroke 37.7mm/Rotation,

Focusing Range 24mm

Nosepiece

Quadruple, Backward With Click Stops

Stage

Double Layer Mechanical Stage 142x132mm, Graphite Surface, Moving Range 75x50mm

Condenser

Abbe Condenser N.A.1.25, Center Adjustable

Light Source

3W LED Brightness Adjustable

Adapter

C-Mount 1.0x

Package

53*32.5*53.5cm/11.5Kg, 1 Set/Carton

9.7" LCD Pad Digital Camera (A59.3520)

LCD

9.7" HD IPS Touch Screen With Backlight

Camera

5.0M 1/2.5" CMOS Digital Camera

Resolution

2048x1536 @15FPS

Pixel

2.2umx2.2um

Data Storage

TF Card or Built-in Memory

Data Output

USB2.0, HDMI, TF Card

Photo

5.0M .JPEG

Video

1080p Video Record

System

Android 5.1 System

90 Languages

Support Mouse + Keyboard Control

Support WIFI

Support Bluetooth

Function

S-Eye Software & Instruction Manual Installed

Mark: Point, Crosshair, Coordinate, Text

Length Measure:

Straight Line Length, Broken Line Length, Curve Length, Parallel Line Distance, Point To

Line Distance

Distance Between 2 Circle Centers:

Radius Fixed Circle, Two-point Fixed Circle, Three-point Fixed Circle

Geometric Measure:

Line Segment Length, Radius Fixed Circle, Two-point Fixed Circle, Three-point Fixed

Circle, Concentric Circle

Geometric Area Measure:

Polygon, Square

Power

DC 12V/2A Power Adaptor, AC 100~240V

Size

Pad 238x182x12mm, Camera 40x39mm

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V. Preparation Before Use

Assemble the Binocular Head

1．Loosen the fixing screw on the top of the microscope body.

2．Put the binocular head on the body properly, then tighten the fixing screw.

Insert the Eyepiece

1．Standard gemel binocular head. Special-processed ring is fixed in the observation tube to tighten the eyepiece.

2．Insert the eyepiece properly.

Assemble the objectives

1．Assemble the objectives clockwise. Make the magnification become bigger when assembling in clockwise

direction.

2．Condenser has been mounted, focused & pre-centered well in the microscope before shipment. There is no need

to do any adjustment.

VI. Usage of the Microscope

Illumination

1．Connect the power line to the microscope.

2．Turn on the power supply, then the light will be on. Brightness can be adjusted by turning the adjusting knob.

Iris diaphragm is designed & configured on condenser. Adjusting the

iris diaphragm to match the different N.A of different objectives.

1．Open/Close the iris diaphragm by adjusting knob.

2．When the knob is on the right, that means the iris diaphragm is fully opened.

Placing the Specimen

1．Put the specimen in the stage and make the cover glass on it. Make the clips clamp them entirely. Two slides can

be put in the stage in the same time.

2．Make the specimen under the objective by adjusting X & Y axis.

Focusing

1．Turn the nosepiece to observe through the lowest magnification.

2．Adjust the stage to the top by turning the coarse focusing knob.

3．Observe through the eyepiece and turn the coarse focusing knob slowly to let the stage down and down till there

is a image

4．Turn the fine focusing knob to get sharp image.

Adjusting of the Binocular Observation Head

1．Adjust the observation tube to make sure that view field in the right & left eyepiece is consistent.

2．Adjust the diopter of the eyepiece to “0” first. Then adjust the eyepiece according to users’ own situation to get

sharp image.

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Oil Immersion

1．When observing through 100X objective, oil immersion is required. Low down the stage to the bottom. Drop the oil

supplied by the manufacturer on the observation area.

2．Turn the 100X objective on its working position.

3．Raise the stage by turning the coarse focusing knob till the front of the objective can touch the oil.

4．Swing back and forth of the nosepiece to get rid of the air bubble in the oil. Then fix the objective. Make sure that

oil should be filled between objective and specimen.

VII. Maintenance of Microscope

When not in Use

1．Handle the microscope with two hands stably, and put the instrument on a stable plane. Cover it up with dust

cover. Store the instrument in a dry place.

2．It is highly recommended that objectives & eyepiece should be kept in a separate container.

Cleaning

1．Keep all the optics clean. It is very important to maintain optical performance of the instrument.

2．If dust, or dirt is covered on the surface of the lens. Have a try of air blower before cleaning the lens.

3．It is recommended that gauze, or lens tissue moistened with methyl alcohol should be used to clean the lens.

Ethyl alcohol is forbidden.

4．When cleaning the low magnification objective, lens tissue or cotton moistened with methyl alcohol can be used.

Pay much more attention when cleaning the high magnification objective. Dry the methyl alcohol first. Do not clean so

hard. Do not use ethyl alcohol.

5．When microscope body has to be taken apart, make sure that do not touch the lens of the objective, or else it will

reduce the image quality.

6．Periodical inspection

To maintain the performance of the microscope, periodical inspection is recommended.

Changing of Bulb

A33.1502 Series biological microscope is configured with LED illumination. There is no need to change bulb if there is no

certain situation.

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LCD Digital Camera (A59.3520)

Real-Time Measurement Software for Android

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1. Introduction

The APP installed in Android Pad A59.3520 can adjust image parameter, measure the objects, capture

image & video from the camera. It also provided simple image processing feature for particles analysis.

2. Camera User Interface

1. Preview Image –show live video of the camera.

2. Tool Panel –Control panels, capture, adjust parameter, measurement, etc.

2.1 Camera parameter adjustment.

2.2 Measurement

2.3 Image and video playback.

2.4 Take pictures and record video.

2.5 Setting

3. Capture and Playback

Click “Snap” button to take a picture. Select picture size in the setting interface.

Click “Record” button to start video recording, click again to stop video recording.

If preview size is smaller than 1080p, the video size is same as preview size. If preview

size is bigger than 1080p, the video size will down to a size smaller than 1080p.

Show picture just captured. Play video file which just saved.

The picture will be opened in Image Analysis interface.

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4. Adjust image parameter of camera

When the color of image is not very good, adjust the effect of image by

“Effect” panel.

Exposure:

Auto mode: Brightness of the image will be automatically

adjusted, you can also adjust the target brightness.

Manual mode: Manually adjust exposure time and gain.

White Balance:

Once AWB: Click icon to trigger once auto white balance.

Manual WB: Manually adjust color temperature.

Color Adjustment

Contrast, Saturation, sharpness and gamma of the image can be

adjusted too.

Click the reset button to restore the paramters.

5. Measurement

5.1 Calibration

We need calibrate the ruler before measurement, Specific combination of magnification of microscope

and the preview size of camera need specific calibration.

Swap the tool pane to measurement, click “Add” to add a new calibration, Click “Edit” to recalibrate the

exist calibration. 1.

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Enter calibration mode

Follow the Tips：

1. Drag the yellow ruler, let endpoints of the ruler close to physical ruler’s scale. We use the 0.01mm

physical ruler, each big grid is 10μm, we pick two grid, that’s 20μm.

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2. Input the name of the calibration and the physical length of the ruler. We input 40x for the name, that

means the magnification of objective is 40X,

Then input the physical length of the ruler, that is 20μm.

3. Click “Calculate” to calculate the calibration value for current objective and preview size, and save to

list.

5.2 Measure Tool

ICON

Function

Description

point

counting

Add a point counting marker on the image.

Line

Measure distance of two points.

Rectangle

Measure width, length and area of rectangle

Circle

Measure area of circle

Cross

Cross hair

Angle

Angle measurement

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