OPTO-EDU A14.1065 User manual

A14.1065

Inverted Biological Phase Contrast Microscope

A16.1065

Inverted LED Fluorescent Microscope, B,G,U

Instruction Manual

This instruction manual is intended for users of the Inverted Series Biological Microscope

A16.1065. To ensure safety use, perform the best performance and to familiarize yourself with

the use of this microscope, please read this manual carefully before operating this product.

Contents

Before Using ……………………………………………………………………

Safety Symbol…………………………………………………………………

Safety Precautions ……………………………………………………………

Care and Maintenance…………………………………………………………

Chapter 1 Introduction …………………………………………………………

1.1 Technical Specifications …………………………………………………

1.1.1 Main Technical Specifications and Configuration……………………

1.1.2 Objective Parameters…………………………………………………

1.1.3 Electrical Parameters…………………………………………………

1.2 Components ………………………………………………………………

Chapter 2 Overview of Agencies ………………………………………………

Chapter 3 Regulation and Operation …………………………………………

3.1 Turning the Power on ……………………………………………………

3.2 Adjusting the Illumination Brightness……………………………………

3.3 Specimen Placement and Stage Knob Adjustment………………………

3.3.1 Mechanical Stage……………………………………………………

3.3.2 Pallet…………………………………………………………………

3.3.3 Auxiliary Stage………………………………………………………

3.4 Adjusting the Interpupillary Distance………………………………………

3.5 Focusing on the Specimens ………………………………………………

3.5.1 Focus Knobs…………………………………………………………

3.5.2 Focus Knob Tight Adjustment ………………………………………

3.6 Adjusting the Diascopic Illumination Aperture Diaphragm………………

3.7 Switching the Objective……………………………………………………

3.8 Operation the Phase Contrast Microscope…………………………………

3.9 Operating the Episcopic Illumination Microscopy………………………………

3.9.1 Eprscopic Illumination………………………………………………

3.9.2 Fluorescence Filter Cube Turret ……………………………………

3.9.3 Filter Cube Switching Unit …………………………………………

3.9.4 Fluorescence Filter Cube……………………………………………

3.9.5 Fluorescence LED Unit ……………………………………………

III

3.10 Operating the LCD ……………………………………………………

3.10.1 The status of LCD …………………………………………………

3.10.2 Operating the illumination switch ………………………………

Chapter 4Assembly………………………………………………………………

4.1 Installation diagram ……………………………………………………

4.2 Installation Steps ………………………………………………………

4.2.1 Installing the Condenser ……………………………………………

4.2.2 Installing the Phase Contrast Slider …………………………………

4.2.3 Installing the Moving Ruler …………………………………………

4.2.4 Installing the Objective ………………………………………………

4.2.5 Installing the Eyepiece ………………………………………………

4.2.6 Installing the Camera…………………………………………………

4.3 Assembly Episcopic Illumination Microscopy…………………………………

4.3.1 Installing the Filter Cube ……………………………………………

4.3.2 Installing the Fluorescence LED Unit ………………………………

4.3.3 Replacing the Fuse …………………………………………………

Chapter 5 Maintenance and Storage……………………………………………

5.1 Cleaning …………………………………………………………………

5.1.1 Cleaning the Lenses …………………………………………………

5.1.2 Cleaning Parts other than Lenses ……………………………………

5.1.3 Cleaning off Immersion Oil …………………………………………

5.1.4 Decontaminating this Product ………………………………………

5.2 Storage ……………………………………………………………………

Chapter 6 Troubleshooting ……………………………………………………

6.1 Optical System ……………………………………………………………

6.2 Mechanical Parts …………………………………………………………

6.3 electrical Parts………………………………………………………………

Before Using A16.1065

Safety Symbol

Symbol

Description

The surface is hot and cannot be touched with bare hands.

Please read this manual carefully before using the product.

Incorrect usage can cause personal injury or property damage.

The main power switch is turned on.

The main power switch is turned off.

Adjustment direction of illumination.

Ground connection.

Up and down light switch.

Light switch.

Through the CE certification.

Lever in，Bino：Trino=100：0

Lever out，Bino：Trino=20：100

Safety Precautions

1. Be careful when opening box, prevent lens glass to stick to fingerprint,

perspiration other factors influence observation, prevent lens and other

attachment drop damaged.

2. Avoid placing the microscope in place with direct sunlight, high temperature

or high humidity, dust, and strong vibration. Ensure that the stage is flat,

horizontal and strong enough.

3. If a bacterial solution or water spatters spill on the stage, objective or

observing tube, immediately unplug the power cord. Then,wipe off any

solution or water with to keep the microscope dry. Otherwise, it could damage

the instrument.

4. To avoid blocking the natural convection air for cooling, make sure the left,

right, top, and rear of the microscope are at least 10 centimeters away from

the wall and other objects.

A16.1065

5. Grounding the machine to avoid lightning strike.

6. To ensure safety, always make sure the main switch is in "O"

(disconnecting) and cut off the power while waiting for the LED lights and

the lamp room to cool completely. Input voltage check: the input voltage

indicated on the back of the microscope is consistent with the supply voltage,

otherwise the microscope will be seriously damaged.

7. Use the our company dedicated wire.

8. When carrying this product, one hand should firmly hold it by gripping the

recess in the front lower part of the main body and the other holds the recess

in the rear of the main body.

★Do not hold any other parts (such as the upper part of the illumination pillar, focus

knobs, eyepiece tube, or stage) when carrying the microscope. Doing so might result in

dropping or failure of this microscope.

Care and Maintenance

1. All lenses have been calibrated, please do not disassemble by yourself.

2. The structure of objective converter and coarse and fine focus mechanism

are precise, please do not disassemble easily.

3. The instrument should be kept clean, have to regular removal of dust, special

attention should be paid to do not contaminate the optical parts when

cleaning.

4. The stain of the lens, such as fingerprints and grease can be wiped with a

small amount of ethyl ether (70%) and alcohol (30%) mixed solution.

Recess in the rear

Recess in the front lower part

A16.1065

5. Do not use organic solvents to clean the non-optical parts of the microscope.

Please use neutral detergents when cleaning.

6. When useing, if the microscope be wet liquid, should immediately cut off

the power and dry.

7. Do not disassemble any part of the microscope, which will affect the

function or reduce the performance of the microscope.

8. The instrument should be placed indoor with cool and dry, covered the dust

cover when not in use. Cover up, must wait for the lamp room to cool down.

9. The microscope usage environmental requirements:

a) For indoor use;

b) The environment temperature range: 10 ℃~ 35 ℃;

c) Maximum relative humidity: 80% at 31 ℃, linear temperature down to

34 ℃is 70%, 60% at 37 ℃, 50% at 40 ℃.

10. Microscope to store and transport environment requirements:

a) the environment temperature range: - 40 ℃~ + 70 ℃

b) relative humidity range: 10% ~ 100%

c) atmospheric pressure range: 500 hpa ~ 1060 hpa

Because the solvent such as ether and alcohol are extremely flammable, do not

operate power switch of various electrical equipment when using, and keep away

from open fire, please ensure indoor ventilation.

Chapter 1 Introduction A16.1065

1.1 Technical Specifications

1.1.1 Main technical specifications and configuration

Device

Technical Specifications

A14.1065

A16.1065

Eyepiece

Extra Wide Field Eyepiece 10×/22

●

Field Eyepiece 15×/16

○

Field Eyepiece 20×/12

○

Observation head

Seidentopf Viewing Head, alterable angle,

Interpupillary 48-75mm

●

Objective

Infinity plane objective 4×

●

○

Infinity plane objective 10×

○

Infinity plane objective 20×

○

Infinity plane objective40×

○

Phase contrast objective 4×

○

Phase contrast objective 10×

●

○

Phase contrast objective 20×

●

○

Phase contrast objective 40×

●

○

Infinity plane semi-apochromatic objective 10×

—

●

Infinity plane semi-apochromatic objective 20×

—

●

Infinity plane semi-apochromatic objective 40×

—

●

Nosepiece

Quintuple Nosepiece

●

Focusing mechanism

up 7mm, down 1.5mm，To limit up to 18.5mm

●

stage

250mm×170mm

●

Moving stage

Y 80mm，X 128mm

●

Plain stage

Two plain stage

○

Condenser

NA=0.3

●

illumination

Transmitted illumination：3WLED

●

Fluorescent illumination：（ B、G、U）3WLED

—

●

Fluorescent illumination：V 3WLED

—

○

Emboss contrast slider

contrast slider

○

Camera mount

C-Mount 1×

●

C-Mount 0.7×

○

C-Mount 0.5×

○

LCD

●

Note：● Standard component, ○ Choose component,―Inapplicability。

A16.1065

Objective Choose component

No.

Technical Specifications

Infinity plane semi-apochromatic objective（adjustable）20×、40×、60×

Infinity plane semi-apochromatic contrast objective（adjustable）20×、40×、60×

Infinity plane semi-apochromatic contrast objective 4×、10×、20×、40×、60×

1.1.2 Objective parameters

Type

A14.1065

A16.1065

Magnification

4×

10×

20×

40×

10×

20×

40×

numerical aperture(N.A)

0.10

0.25

0.40

0.60

0.30

0.45

0.60

Work distance (mm）

10.2

2.2

7.4

3.6

Cover-glass thickness

0.17

1.2

Conjugate distance(mm)

∞

1.1.3 Electricity parameter

Item

A14.1065

A16.1065

Input voltage

AC100-240V; 50/60 Hz

Fuse Size

T500 mA; 250 V

LED light

3W S-LED

Fluorescence LED light (B light）

—

wavelength is 485nm at 3W

Fluorescence LED light (G light)

—

wavelength is 520nm at 3W

Fluorescence LED light (U light)

—

wavelength is 360nm at 3W

A16.1065

1.2 Components

The microscope A16.1065 components ( following diagrams) .

A16.1065

Viewing head

Camera mount

LCD

Mainframe

Nosepiece

Stage

Condenser

Objective

Contrast slider

Moving stage

Eyepiece

A16.1065

The microscope A16.1065-FL components ( following diagrams).

A16.1065-FL

Viewing head

Camera mount

LCD

Mainframe

Fluorescence

illumination

Stage

Condenser

Filter Cube turret

Fluorescent baffle

Eyepiece

Nosepiece

Objective

Moving stage

Upper lighting

shading part

Contrast slider

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