Nippon Genetics FastGene User manual

NIPPON Genecs EUROPE GmbH Mariaweilerstraße 28-30, 52349 Düren, Germany,

Tel: +49 2421 5549 60, Fax: +49 2421 55496 11, E-Mail: info@nippongenecs.de, www.nippongenecs.eu

FAS-BG LED BOX

GP-04LED Manual

NIPPON Genecs EUROPEFastGene® FAS-BG LED BOX

Content

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1. GENERAL INFORMATION......................................................................................................

1.1 Warranty.................................................................................................................

 1.2Regulatorynoce..................................................................................................

 1.3Safetyinformaon................................................................................................

 1.4Contactinformaon............................................................................................

2. TECHNICAL SPECIFICATIONS...............................................................................................

3. SYSTEM OPERATION...............................................................................................................

 3.1Productcontentandoverview...........................................................................

 3.2Inialsetup.............................................................................................................

 3.3Agarosegelscanning(Blue/GreenLEDlight).................................................

 3.3.1Gelposioning...................................................................................................

 3.3.2Exposuremecorrecon.................................................................................

 3.3.3Imageviewermenuandexternalstoragetransfer....................................

 3.4DNAextraconfromagarosegels...................................................................

 3.5Membrane/Petridishscanning(Whiteepilight)...........................................

 3.6Colorimetricgelscanning(WhiteLEDlighttransilluminator)....................

 3.7.Generalsengs...................................................................................................

 3.8.Connecnganexternalprinter.........................................................................

 3.9.Connecnganexternal(touch)monitor.........................................................

NIPPON Genecs EUROPEFastGene® FAS-BG LED BOX

1. GENERAL Informaon

Thankyouforpurchasingthe FastGene®FAS-BGLEDBOXImagingSystem,astand-alonegeldocumen-

taonsystem.TheBlue/GreenLEDtechnologypermitsthedeteconofDNAwithhighestsensivityand

withoutharmingyoureyes,skinoryoursample.WiththewhiteLEDlightyoucanimageproteingelsstained

withcoomassieorsilverstaining.ThewhiteLEDepi-illuminaonallowsthedocumentaonofopaquesur-

facessuchasPetridishesorWesternBlotmembranes.

PleasereadcarefullythefollowingnotestobeabletoproperlyusetheFastGene®FAS-BGLEDBOXImaging

System.

1.1 Warranty

TheFastGene®FAS-BGLEDBOXiswarrantedagainstdefectsinmaterialsandworkmanshipfor1year.In

caseofanydefectsoccurringintheinstrumentoraccessoriesduringthiswarrantyperiod,NIPPONGenecs

EUROPEwillrepairorreplacethedefecvepartsatitsdiscreonwithoutcharge.

The following defects, however, are specically excluded:

 -Defectscausedbyimproperoperaon.

 -RepairormodicaondonebyanyoneotherthanNIPPONGenecsEUROPE

  oranauthorizedagent.

 -Damagecausedbysubstungalternaveparts.

 -UseofngsorsparepartssuppliedbyanyoneotherthanNIPPONGenecsEUROPE.

 -Damagecausedbyaccidentormisuse.

 -Damagecausedbydisaster.

 -Corrosioncausedbyimpropersolventorsample.

Foranyinquiryorrequestforrepairservice,contactNIPPONGenecsEUROPEoryourlocaldistributor.

Pleasesendamessagecontaininginformaonaboutthemodelandserialnumberofyourinstrument.

1.2 Regulatory noce

IMPORTANT:

ThisNIPPONGenecsEUROPEinstrumentisdesignedandceredtomeetsafetystandardsandEMC

regulaons.Ceredproductsaresafetousewhenoperatedinaccordancewiththeinstruconmanual.

Thisinstrumentmustnotbemodiedoralteredinanyway.Alteraonofthisinstrumentwill:

Voidthemanufacturer’swarranty

VoidthesafetyandEMCcercaon

Createapotenalsafetyhazard

NIPPONGenecsEUROPEisnotresponsibleforanyinjuryordamagecausedbytheuseofthisinstrument

forpurposesotherthanthoseforwhichitisintended,orbymodicaonsoftheinstrumentnotperformed

byNIPPONGenecsEUROPEoranauthorizedagent.

NIPPON Genecs EUROPEFastGene® FAS-BG LED BOX

1.3 Safety informaon

Pleasecarefullyreadthefollowingsafetyinformaonnotestobeabletoproperlyusethe

FastGene®FAS-BGLEDBOX:

Please wear appropriate personal protecve equipment. Please read the instrucons manual to properly

usetheFastGene®FAS-BGLEDBOX.UsinginstrumentswithFastGene®Blue/GreenLEDtechnologytrans-

illuminatorswillnormallynotdamagesamplesoreyesandskinoftheuser.However,prolongedexposure

ofhumannakedeyeswithlightirradiaoninthebluespectrummayincreasetheprobabilityofsuering

fromrenadiseases.Therefore,werecommendusingtheamberltershieldincludedinthepackage,when

handlingtheBlue/GreenLEDtransilluminatorwhiletheFastGene®FAS-BGLEDBOXisopenandtheBlue/

GreenLEDlightisturnedon.

OperatetheFAS-BGLEDBOXonlyifitisconnectedtoasafetysocket.

Placetheunitonalevelsurfacewithminimalchanceofdropping.

1.4 Contact informaon

NIPPONGenecsEUROPEGmbH

Mariaweilerstraße28-30

52349Düren,Germany

Tel: +49 2421 554960

Fax: +49 2421 55496 11

E-Mail:info@nippongenecs.de

Formoredetailedproductinformaon,contactdetails,quesons,ortroubleshoongplease

visitourwebsitewww.nippongenecs.euorcontactusviainfo@nippongenecs.de.

FastGene®isaregisteredtrademarkofNIPPONGenecsEUROPE GmbH.

NIPPON Genecs EUROPEFastGene® FAS-BG LED BOX

2. Technical Specicaons

Lightsources Blue/GreenLEDtransilluminator

Whiteepilight

WhiteLEDlighttransilluminator

Transilluminatedarea 16x11,5cm

Light sources

General Info

Power 12V,4.16A

Dimensions(HxDxW) 23x25.4x20.7cm

GelTools Amberltershield

Weight 3.2kg

Catalog number

GP-04LED FAS-BGLEDBOX

Image capture

Sensor CMOSSensor

Camera 8MPixelcamera

Image format TIFF,JPEGandPNG

Exposureme 0.2-2sec,21exposuresteps

Display/Soware/Connecons

Display 5"ColorLCDTouchPanel

Soware Inbuiltcontrolsoware

Connecons 2xUSBPort(1xfront,1xback)

1xHDMIPort

Thermalprintersupport

NIPPON Genecs EUROPEFastGene® FAS-BG LED BOX

DC power jack HDMI port

USB 2.0 port

GP-04LED Product Content

FAS-BGLEDBOX

Usermanual

A/CPowerTransformer(12V,4.16A,DC)

Amberltershield

Blackvelvetsheet

3.1 Product content and overview

3. System Operaon

Upper lid

Power buon

5" LCD Touch Screen

and control menu

USB 2.0 port

Light source switch

Amber lter shield

Black velvet sheet

NIPPON Genecs EUROPEFastGene® FAS-BG LED BOX

3.2 Inial setup

Removealltapesthatholditemsinplaceforshipping.PlacetheFastGene®FAS-BGLEDBOXunitonaat

surface.Connecttheincluded DCadaptertothe backoftheunit thenplug intopoweroutlet. Pushthe

powerbuon.

Iniate the desired applicaon

Agarosegelscanning(Blue/GreenLEDlight)(secon3.3)

DNAextraconfromgel(secon3.4)

Membrane/Petridishscanning(Whiteepilight)(secon3.5)

Colorimetricgelscanning(WhiteLEDlight)(secon3.6)

3.3 Agarose gel scanning (Blue/Green LED light)

3.3.1 Gel posioning

OpenthelidoftheFastGene®FAS-BGLEDBOX.Slidetheblackvelvetsheetintothegrooveofthelidto

coverthewhitelightareaofthelid(seegurebelow).Theblackvelvetsheetreducesthebackground.

Placethegeltraywiththeagarosegelontheviewareaandclosethelid.Note:theviewareaiswater-

proof.Hence,theusageofthegeltrayisnotobligatory.

Upper lid Insert black velvet sheet

NIPPON Genecs EUROPEFastGene® FAS-BG LED BOX

Slidethelightsourceswitchonthefrontsideoftheinstrumenttothecyanlightsource(seegurebelow).

Press„B/G“(Blue/GreenLEDlight)onthecontrolmenu,displayedontherightsideofthescreen.

ThiswillturnontheBlue/GreenLEDlight.Aliveimageofthegelwillbedisplayedonthescreen.

3.3.2 Exposure me correcon

Usetheupanddownbuonsonthecontrolmenutochangeandcorrecttheexposuremeunltheappro-

priateexposureofthegelisreached.Theexposuremecanbeadjustedfrom-10(shortexposureme)to

+10(longexposureme).Themiddlebuondisplaysthecurrentexposurelevel(e.g.-2)

Capture

-2

Exposure

B/G WL

epi WL

back

Connuous

Capture

Seng Image

Viewer

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