Nikon DIAPHOT 300 User manual

Ill

Instructions

NIKON

CORPORATION

Thank

you

for

your

purchase

Nikon’s

Inverted

Microscope

Diaphot

300.

Please

read

this

instruction

manual

thoroughly

order

become

acquainted

with

the

complete

system

and

its

operation.

hope

your

Diaphot

300

will

lasting

service.

Nikon

reserves

the

right

make

such

altera¬

tions

design

may

considered

necessary

the

light

experience.

For

this

reason,

particulars

and

illustrations

this

handbook

may

not

conform

every

detail

models

current

production.

Handling

Precautions

Handle

Carefully!

Handle

the

microscope

gently,

taking

care

avoid

sharp

impacts.

Purpose

Use

the

microscope

only

for

microscopic

observation.

not

use

for

any

other

applications.

Microscope

Location

Select

location

with

limited

exposure

dust,

vibration,

high

temperature

and

humidity

(40°C,

80%

more),

and

direct

sunlight.

Leave

certain

space

between

the

microscope

and

the

nearby

wall

allow

the

user

look

the

warning

labels

the

lamphouse.

12V100W

Power

Supply

Unit

The

power

supply

unit

specified

page

"ELECTRICAL

SPECIFICATIONS"

the

power

supply

unit

light

the

Nikon

microscopes'

2V100W

lamp.

not

use

for

other

purposes.

Also,

the

user

may

not

use

the

power

supply

unit

broken,

and

may

not

open

the

cover

the

power

supply

unit.

Line

Voltage

Confirm

that

the

input

voltage

the

2V1

00W

power

supply

unit

corresponds

your

line

voltage.

sure

use

the

specified

power

supply

unit

(described

page

"ELECTRICAL

SPECIFICATIONS").

Light

Source

The

microscope

uses

2V,

1OOW

halogen

lamp

bulb

the

standard

light

source.

not

use

any

lamp

not

described

the

"ELECTRICAL

SPECIFICATIONS"

(on

page

53).

lamp

bulb

over-rated

wattage

used,

the

light

adjusting

circuit

may

damaged.

With

Lamp

—CAUTION

—

not

touch

the

lamphouse

place

any

heat-sensitive

object

near

it,

since

the

lamphouse

becomes

extremely

hot

during

use.

Also

use

great

care

not

bring

volatile

substances

(such

gasoline,

thinner,

alcohol,

etc.)

the

lamphouse.

Such

exposure

may

result

inflammation

other

dangers.

Replacing

the

Halogen

Lamp

Before

replacing

the

12V,

100W

halogen

lamp,

turn

off

the

power

switch

and

unplug

the

power

cord.

When

replacing

the

halogen

lamp,

wait

until

cools

down.

not

touch

the

glass

the

halogen

lamp

with

bare

hands.

Dirt

Lens

not

leave

dust,

dirt

fingerprints

the

lens

lamp.

Dirt

stains

the

lens

mirror

will

deteriorate

image

quality.

10.

Focusing

Knobs

Never

attempt

turn

the

right-hand

and

left-hand

focusing

knob

opposite

directions

the

same

time

will

result

defects

damages.

attempt

turn

the

coarse

focusing

knob

further

after

reaches

its

limit

will

cause

defects

damages.

Never

turn

forcedly.

Care

and

Maintenance

Lens

Cleaning

Dust

best

removed

with

soft

brush

gauze.

persistent

dirt,

such

fingerprints,

grease

and

oil,

may

removed

with

soft

cotton,

lens

tissue,

gauze

lightly

moistened

with

absolute

alcohol

(methyl

alcohol

ethyl

alcohol).

Use

only

xylene

clean

immersion

oil

off

objective

surfaces.

not

use

xylene

clean

the

entrance

lens

the

bottom

the

eyepiece

tube

prism

surface

the

eyepiece

tube.

Absolute

alcohol

and

xylene

are

quite

inflammable.

Use

great

care

when

handling

them

and

when

setting

the

power

switch

and

off.

very

careful

with

fire.

Cleaning

Painted

Surfaces

Avoid

use

any

organic

solvents

(such

alcohol,

ether,

thinner,

etc.)

clean

the

painted

plastic

surfaces

the

instrument.

recommend

that

these

parts

cleaned

with

silicon

cloth.

Never

Dismantle

Never

attempt

dismantle

the

instrument,

thereby

avoiding

the

possibility

impaired

operational

efficiency

and

accuracy.

When

Not

Use

When

the

microscope

not

use,

cover

with

the

vinyl

cover,

and

store

dry

place

not

subject

mold.

especially

recommend

that

the

objectives

and

eyepieces

kept

container

(such

desiccator)

with

desiccant

it.

Periodical

Inspection

maintain

the

performance

the

microscope,

recommend

you

check

the

microscope

periodically.

(For

details

inspection,

contact

your

dealer

nearest

Nikon

representative.)

NOMENCLATURE

CONTENTS

Handling

Precautions

Care

and

Maintenance

NOMENCLATURE.

II.

BASIC

MICROSCOPY.

III.

PHOTOMICROGRAPHIC

PROCEDURE.

IV.

OPERATIONS

COMPONENTS

ON/OFF

switching

power.

Adjusting

Brightness.17

Changing

Optical

Paths.19

Filters.20

Field

Aperture

Diaphragm

Condenser

Aperture

Diaphragm

System

Condenser

Turret

Unit

Objectives.26

Turret

Assembly.28

10.

Diopter

Adjustment.29

Adjusting

Interpupillary

Distance

.30

Photomask.31

Eyepiece

tube.32

14.

Focusing

Device.33

15.

Refocusing

Stopper

16.

Handling

Condenser

Flolder

17.

Tiltable

Illumination

Pillar.36

18.

Rectangular

Mechanical

Stage.38

19.

Photomicrography.39

ASSEMBLY

VI.

SYSTEM

DIAGRAM

VII.

TROUBLESHOOTING

•

ELECTRICAL

SPECIFICATIONS

II.

BASIC

MICROSCOPY

III.

PHOTOMICROGRAPHIC

PROCEDURE

IV.

OPERATIONS

COMPONENTS

ACCPMRIV

MOO

VIDL

VI.

SYSTEM

DIAGRAM

YU.

TROUBLESHOOTING

•

ELECTRICAL

SPECIFICATIONS

NOMENCLATURE

System

Components

(Right-hand)

Supplementary

lens

pocket

Field

aperture

diaphragm

lever

Figure

1-1

System

Components

(Left-hand)

Condenser

refocusing

stopper

Condenser

mount

rotation

clamp

screw

Condenser

holder

Module

Condenser

turret

Annular

diaphragm

centering

screw

Condenser

lens

Objective

Revolving

nosepiece

Side

port

Coaxial

coarse/fine

focusing

knob

Refocusing

stopper

Light

intensity

control

dial

Condenser

mount

Condenser

centering

screw

System

condenser

turret

unit

Bertrand

lens

focusing

screw

Eyepiece

tube

port

Front

port

Figure

-2

System

Components

(Rear)

Lamphouse

cable

connector

Figure

1-3

II.

BASIC

MICROSCOPY

For

the

normal

microscopy,

carry

out

steps

14.

For

phase

contrast

microscopy,

carry

out

steps

18.

For

detailed

operations,

see

Section

IV.

"OPERATIONS

COMPONENTS

from

page

16.

the

microscope

has

not

been

assembled,

first

read

Section

"ASSEMBLY"

from

page

42.

urn

the

power.

After

confirming

that

the

power

supply

set

for

the

correct

line

voltage,

turn

the

power

switch.

Set

the

light

intensity

control

dial

the

power

supply

unit

CTRL.

Figure

2-1

the

lamp

voltage

Rotate

the

light

intensity

control

dial

the

left

side

the

base

the

"6"

position.

(If

the

light

intensity

too

high

too

low,

adjust

properly.)

the

optical

path

for

the

observation.

Set

the

turret

assembly

(Eyepiece

tube

BT1

only.)

Set

the

optical

path

changeover

dial

the

right

side

the

base

Figure

2-3

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