Nikon Eclipse E400 User manual

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1

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Instructions

Before

Using

pr

nnn

eno

con

-

i

ーーー

Thank

you

for

purchasing

this

Nikon

product.

This

instruction

manual

is

written

for the

users

of

Nikon

Microscope

ECLIPSE

E400.

To

ensure

correct

usage

read

this

manual

carefully

before

operating

the

instrument.

@

It

is

prohibited

to

reproduce

or

transmit

this

manual

in

part

or

whole

without Nikon's

expressed

permission.

©

The

contents

of

this

manual

are

subject

to

change

without

any

notice.

@

Although

every

effort

has

been

made

to

ensure

the

accuracy

of

this

manual,

if

you

note

any

points

that

are

unclear

or

incorrect,

contact

your

nearest

Nikon

representative.

—

—

Warning/Caution

Symbols

in

This

Manual

Though

Nikon

products

are

designed

to

provide

you

utmost

safety

during

use,

incorrect

usage

or

disregard

of

the

instructions

can

cause

personal

injury

or

property

damage.

For

your

safety,

read

the

instruction

manual

carefully

and

thoroughly

before

usage.

Do

not

discard

this

manual

but

keep

it

near

the

product

for

easy

reference.

Inside

this

instruction

manual,

safety

instructions

are

indicated

with

the

symbols

shown

below.

Be

sure

to

follow

the

instructions

marked

with

these

symbols

for

your

safety.

Symbol

Contents

Disregarding

instructions

marked

with

this

symbol

may

lead

to

death

or

WARNING

oo,

serious

injury.

Disregarding

instructions

marked

with

this

symbol

may

lead

to

injury

or

CAUTION

property

damage.

——

Symbol

on

the

product

Symbol

Contents

LN

Caution

for

heat

—

WARNING

ZE

Intended

product

use

This

microscope

should

only

be

used

for

microscopic

observation.

Do

not

use

it

for

any

other

purpose.

XE

L

Do

not

disassemble

Disassembly

may

cause

malfunction

and/or

electrical

shock.

Do

not

disassemble

any

parts

other

than

those

mentioned

in

this

manual.

If

you

notice

any

malfunction,

notify

your

nearest

Nikon

representative.

ο

Confirm

the

input

voltage

Make

sure

that

the

input

voltage

indicated

on

the

back

panel

of

the

microscope

is

the

same

as

your

regional

voltage.

If

not

the

same,

do

not

use

the

microscope;

instead,

notify

your

nearest

Nikon

representative.

If

the

microscope

is

used

with

the

wrong

input

voltage,

a

short

circuit

or

fire

may

result,

causing

the

damage

of

the

microscope.

Power

cord

To

prevent

electrical

shock,

always

turn

off

the

power

switch

(flip

it

to

the

©”

side)

before

connecting

or

disconnecting

the

power

cord.

Use

only

the

power

cord

provided

with

the

microscope.

(Refer

to

p.

24.)

Using

the

wrong

power

cord

could

result

in

damage

or

fire.

$,

eat

from

the

light

source

The

lamp

becomes

extremely

hot.

To

avoid

burns,

do

not

touch

the

lamp

and

the

area

around

it

while

the

lamp

is

lit

or

for

about

thirty

minutes

after

the

lamp

has

been

turned

off.

Furthermore,

in

order

to

avoid

the

risk

of

fire,

do

not

place

fabric,

paper

or

highly

flammable

materials

(such

as

gasoline,

petroleum

benzine,

paint

thinner

or

alcohol)

near

the

lamp

while

it

is

lit

or

for

about

thirty

minutes

after

it

has

been

turned

off.

The

back

of

the

microscope

also

becomes

hot

during

use.

Although

this

is

not

a

malfunction,

be

careful

not

to

touch

the

back

of

the

microscope

when

it

is

hot.

CAUTION

E

Installation

location

This

product

is

a

precision

optical

instrument.

Using

or

storing

the

instrument

under

unsuitable

conditions

may

damage

it

or

may

have

an

adverse

effect

on

its

precision.

The

following

conditions

should

be

kept

in

mind

when

selecting

the

installation

location.

©

Avoid

installing

in

a

place

exposed

to

direct

sunlight,

or

directly

under

room

lights

or

any

other

bright

place.

The

quality

of

the

view

through

the

microscope

deteriorates

in

bright

surroundings.

@

Install

in

a

place

with

little

dust

and

dirt.

©

Install

in

a

level

place

free

from

vibrations.

©

Install

on

a

sturdy

desk

or

table

that

is

able

to

bear

the

weight

of

the

microscope.

O

Do

not

install

in

a

warm

and

humid

location.

Otherwise,

condensation

or

mold

may

form

on

the

lenses,

degrading

performance

or

damaging

the

equipment.

Check

the

light

source

The

power

supply

contained

in

the

microscope

provides

the

power

for the

halogen

lamp

as

the

microscope

light

source.

Although

this

power

supply

can

power

a

halogen

lamp

of

up

to

6

V-30

W,

the

specified

lamp

must

be

used.

Use

of

a

non-specified

lamp

could

damage

the

microscope.

Specified

lamp:

6

V-30

W

(PHILIPS

5761)

Cautions

when

replacing

the

lamp

In

order

to

prevent

burns,

allow

the

lamp

to

cool

(for

at

least

thirty

minutes

after

turning

off

the

power)

before

replacing

the

lamp.

Furthermore,

to

prevent

electrical

shock

and

damage

to

the

microscope,

always

turn

off the

power

switch

(flip

it

to

the“

”

side)

and

unplug

the

power

cord

before

replacing

the

lamp.

After

replacing

the

lamp,

be

sure

to

attach

the

lamp

cover

securely.

Never

use

the

lamp

without

its

cover.

Cautions

when

operating

the

stage

The

stage

rack

protrudes

outward

when

the

stage

is

moved.

When

operating

the

focus

knobs

or

condenser

focus

knob,

be

careful

not

to

bump

your

hand

on

the

rack

since

the

end

of

the

rack

may

injure

your

hand.

n

em

ta

aray

mmm

oem

araras

rg

..

arm

ne

-en

P,

vi

-

CAUTION

¿

Я

Do

not

wet

Spilling

water

on

the

microscope

may

cause

electrical

short

circuits,

resulting

the

microscope

failure

or

abnormal

heat

generation.

If,

by

mistake,

water

or

liquid

is

spilled

on

the

microscope,

immediately

turn

off

the

power

switch

(flip

it

to

the

“ ”

side)

and

unplug

the

power

cord.

Then

use

a

dry

cloth

to

wipe

away

the

moisture.

If

any

liquid

gets

inside

of

the

microscope,

do

not

attempt

to

use

it;

instead,

contact

your

nearest

Nikon

representative.

x

Weak

electromagnetic

waves

This

microscope

emits

weak

electromagnetic

waves.

The

accuracy

of

any

precision

electronic

equipment

may

be

adversely

affected

if

positioned

near

this

microscope.

If

this

microscope

affects

TV

or

radio

reception,

move

the

radio

or

TV

away

from

the

microscope.

χει...

on

carrying

the

microscope

Securely

hold onto

the

microscope

with

the

base

of

the

arm

from

the

back

of

the

microscope.

Because

this

microscope

is

a

precision

optical

device,

handle

it

carefully

and

do

not

subject

it

to

strong

physical

shocks.

(The

precision

of

the

objective

in

particular

may

be

adversely

affected

by

even

weak

physical

shocks.)

In

addition,

do

not

grasp

the

focus

knobs,

the

eyepiece

tube,

the

stage,

etc.,

when

carrying

the

microscope;

there

is

a

chance

that

the

part

could

come

off,

and

the

microscope

could

be

damaged.

When

picking

up

the

microscope

or

putting

it

down,

be

careful

not

to

bump

the

lamp

cover

on

the

back

panel

against

other

things.

^7

Names

of

Component

Parts

and

Operational

Parts

Eyepiece

tube

Eyepiece

Diopter

adjustment

ring

Specimen

holder

Condenser

clamp

screw

Eyelevel

riser

Condenser

focus

knob

Revolving

nosepiece

Coarse

torque

/

tm

adjustment

ring

(TORQUE)

/

я

Objective

TORQUE)

|

イイ

1

1620

コ

0

бе

ER

AVE

|

Coarse

focus

knob

!

Fine

focus

knob

Brightness

adjuster

(LAMP)

Stage

rotation

clamp

screw

Auto-photo

switch

Auto-photo

voltage

ae

|

2488

selector

switch

г

Eyepiece

tube

clamp

screw

Eyelevel

riser

clamp

screw

n

\

Coarse

focus

stopper

1

ring

(CLAMP)

Fine

focus

knob

Condenser

aperture

diaphragm

ring

Condenser

Condenser

centering

screw

Power

switch

Y-axis

stage

motion

Field

diaphragm

ring

control

knob

X-axis

stage

motion

Field

lens

control

knob

Tool

holder

Rating

label

AC

input

connector

=

|

|

TS

HE

|

Lamp

cover

2

=

4

7

Power

cord

Back

panel

Proper

Use

Makes

a

Diffe

or

nan

ee

a

h

obtained

with

an

aperture

SHE

Έλις

αμ

ΕΠΟ:

ould

be

made

whenever

the

abp

tas

POS

Excessped

con

details

canin

be

süz

Proper

improper

adjustment

t

yt

&

Effects

of

Condenser

Focusing

and

Centering

Center

the

condenser

so

that

the

image

of

the

field

diaphragm

appears

clearly

in

the

center

of

the

view

field.

Hf

not

centered

he

field

diaphragm

will

be

shifted

out

of

position

and

resolution

will

be

reduced.

Always

make

P.27

correctly,

the

center

of

i

these

adjustments

when

assembling

the

microscope.

Off-center

SE

|

Out

of

focus

_

Extremely

out

of

focus

ob

_

Correct

adjustment

ーー

roll

|

ian

tt

ně

Image

of

the

field

-

Image

of

the

field

Extremely

poor

diaphragm

is

off-centered

Image

of

the

field

diaphragm diaphragm

appears

fuzzy

image

quality

appears

clearly

m

the

center

of

the

view

field

Effect

of

Color

Balancing

Filter

(NCB11)

if

specimens

are

observed

with

illumination

from

the

lamp

alone,

their

images

are

seen

with

a

yellow

tint.

Using

the

NCBI

filter

gives

the

images

a

natural

color

tone.

This

filter

is

normally

used

when

observing

specimens

by

bright

8

PAG

field

microscopy.

Without

using

the

NCB11

filter

r

Yellowish

color

tone

Using

the

NCB11

filter

Approximates

natural

daylight

color

Effect

of

Lamp

Voltage

Adjustment

ht

becomes

redder.

Conversely.

if

the

voltage

is

increased,

the

d

using

the

NCB11

filter

results

in

the

most

If

the

lamp

voltage

is

decreased,

the

color

tone

of

the

hig

color

tone

of

the

light

becomes

bluer.

Pressing

the

auto-photo

switch

an

natural

color

tone.

ms

P.18

Low

lamp

voltage

z

High

lamp

voltage

-photo

switch

on

nu

e

Reddish

color

tone

Auto-photo

Swite

Bluish

color

tone

Approximates

natural

daylight

color

——%

_Contents

e

e

eee

Before

Using

Names

of

Component

Parts

and

Operational

Parts

Proper

Use

Makes

a

Difference

Contents

Preparations

for

Microscopy

Placing

a

Specimen

on

the

Stage

Changing

Magnification

Photomicrography

Locking

the

Focus

Position

Changing

the

Torque

of

the

Coarse

Focus

Knob—

Changing

the

Torque

of

the

Stage

Motion

Control

Knobs

Selecting

a

Condenser

Assembling

and

Disassembling

the

Microscope

—

Replacing

the

Lamp

Installing

the

Condenser

Installing

the

Stage

Installing

the

Objectives

Installing

the

Eyelevel

Riser

(If

necessary)

一

一

一

Installing

the

Eyepiece

Tube

Installing

the

Eyepieces

Lens

Cleaning

Keeping

the

Microscope

Clean

Storage

Regular

Inspections

Troubleshooting

Tables

Specifications

and

Ratings

Index

1-4

10-11

13

14

15

16

17—18

19

20

21-22

23-25

26

27

28

28

29

30

30

30

31

31

32-34

35

36