Motic AE31E Series User manual

We are constantly endeavouring to improve our instruments and to adapt them to the requirements of

modern research techniques and testing methods. This involves modification to the mechanical

structure and optical design of our instruments.

Therefore, all descriptions and illustrations in this instruction manual, including all specifications are

subject to change without notice.

Content

1. Nomenclature 4

1.1 Application 4

1.2 Nomenclature 5

1.3 Technical data 7

2. Setting-up the Instrument 8

2.1 Working Environment 8

2.2 Input voltage and power 8

2.3 Illumination 9

2.3.1 Halogen bulb 9

2.3.2 LED module 9

3. Assembling the Microscope 10

3.1 Installing the bulb/ LED module 10

3.1.1 30W Halogen 10

3.1.2 100W Halogen 11

3.1.3 LED module 12

3.2 Filter Holder 14

3.3 Mounting the Condenser 14

3.4 Installing the Objectives 15

3.5 Mechanical Stage 16

3.6 Inserting the Eyepieces 16

4. Microscope Handling 17

4.1 Interpupillary Distance Adjustment 17

4.2 Diopter Adjustment 17

4.3 Coarse and fine focusing 18

4.4 Coarse focus torque adjustment 18

4.5 Brightfield Microscopy 19

4.6 Phase Contrast Microscopy 19

4.7 Filter selection 22

4.8 Brightness control 22

4.9. IR function 23

4.10 Auto light intensity reproduction 23

5. Photomicrographic Procedure (Only on AE31E TRI) 24

6. Troubleshooting Table 25

6.1 Optical and Operating Problems 25

6.2 Electrical 25

7. Care and Maintenance 26

7.1 Lenses and Filters 26

7.2 Cleaning of Painted or Plastic Components 26

7.3 When Not in Use 26

7.4 Warning Label 27

1. Nomenclature

1.1 Application

The new Inverted microscope AE31E is representing the ideal instrument for living cells

observation and other microbiological samples. Due to our Colour Corrected Infinity Optics (CCIS)

the AE31E offers superior image quality combined with excellent mechanical performance. Daily

work is facilitated by a number of ergonomic features.

1.2 Nomenclature

Model AE31E TRI

(Fig.1)

Model AE31E TRI

(Fig.2)

1.3 Technical data

Technical data AE31E BIN AE31E TRI

Optical system Color Corrected Infinity Optics (CCIS)

Total Magnification 40X - 400X

Eyepiece tubes

360° Swiveling Siedentopf with upper and lower position: upper

position offers approx. 40 mm extra viewing height

Interpupillary distance: 48 mm to 75 mm

Binocular 45° Inclined Trinocular 45° Inclined

Eyepiece N-WF 10X(Ø22), wide field, high eye point

Nosepiece Quintuple, inclined, sidewards

Stage Fixed, Dimensions (width x depth): 200 x 239mm

Stage height 192mm

Object guide Verniers with numerical and alphabetic scale

X direction: numerical scale, readable from right to left

Y direction: alphabetic scale

Objectives

Plan-Achromat 4x, N.A.0.1, W.D.12.6mm

Plan-Achromat 10x, N.A.0.25, W.D.16.8mm

LWD Plan-Achromat 20x, N.A.0.3, W.D.4.7mm

LWD Plan-Achromat 40x, N.A.0.5, W.D.3mm

Plan-Achromat PH 4x Ph0, N.A.0.1, W.D.12.6mm

Plan-Achromat PH 10x Ph1, N.A.0.25, W.D.4.1mm

LWD Plan-Achromat PH 20x Ph1, N.A.0.3, W.D.4.7mm

LWD Plan-Achromat PH 40x Ph1, N.A.0.5, W.D.3mm

LWD Plan-Achromat PH 40x Ph2, N.A.0.5, W.D.3mm

Condenser N.A.0.3, W.D.72mm

N.A.0.5, W.D.28mm

Coarse focus 42mm/rot.

Fine focus 0.2mm/rot.

Transmitted illumination 6V/30W Halogen

12V/100W Halogen

3W LED module

Dimension: W x D x H 217.5 x 556 x 497mm

v2. Setting-up the Instrument

2.1 Working Environment

The location should be free from dust, moisture, chemical vapours and free of mechanical

vibrations.

Don't locate the instrument in bright or direct ambient light, in front of a lamp, or a bright light wall.

Best imagequality will be achieved in a surrounding surrounding with low ambient light.

Environmental specification:

Indoor use

Altitude: Max 2000m

Ambient temperature: 15°C~ 40°C;

Maximum relative humidity: 75% for temperature up to 31°C decreasing linearly to 50% relative

humidity at 40°C

Supply voltage fluctuations: Not to exceed ±10% of the normal voltage

Pollution degree: 2 ( in according with IEC60664)

Installation/Overvoltage category: 2 (in according with IEC60664)

Air Pressure of 75kPa to 106 kPa

2.2 Input voltage and power

Automatic voltage selection works with electrical outlets worldwide. It is advised to always use a

power cord that is rated for the voltage used in your area and that has been approved to meet

local safety standards. Using the wrong power cord could cause fire or equipment damage.

In order to prevent electric fluctuation to the instrument electrics, always turn the power switch on the

instrument off before connecting the power cord.

Electrical Specifications:

30W Halogen bulb

Input: 100-240V~, 45W, 50-60Hz

Lamp: DC6V 30W Halogen

Fuse: 250V T2.5A

(If the original fuse is damaged, please replace a new one with same specification)

100W Halogen bulb

Input: 100-240V~, 120W, 50-60Hz

Bulb: 12VDC 100W Halogen

Fuse: 250V T2.5A (If the original fuse is blown, please replace with specified fuse)

LED module

Input: 100-240V~, 80VA, 50-60Hz

LED: 3.4V 3W

Fuse: 250V T2.5A (If the original fuse is blown, please replace with specified fuse)

LED module high color temperature: 6000 ± 300k

LED module low color temperature: 4500 ± 300k

Attention: The plug of the power adaptor is the "disconnect device" for whole unit. To save energy we

recommend to unplug the instrument when not in use.

This device complies with Part 15 of the FCC Rules.

Operation is subject to the following two conditions:

(1) This device may not cause harmful interference, and (2) this device must accept any interference

received, including interference that may cause undesired operation.

2.3 Illumination

2.3.1 Halogen bulb

The quartz halogen bulb, used as a light source, has higher luminance and colour temperature

than conventional tungsten lamps. The luminance of halogen bulb is approximately four times

brighter than the conventional tungsten lamps.

As long as the lamp voltage is kept constant, the halogen lamp maintains the same level of

brightness and colour temperature regardless of whether it is new or nearing the end of its

life span.

2.3.2 LED module

The LED module is specially designed to be inserted into halogen bulb socket directly converting

halogen illumination to LED illumination. LED is more economical and environmental friendly and

combines the advantages of low heat and long life span.

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