Miltenyi Biotec UltraMicroscope II User manual
UltraMicroscope II Imaging System
User manual
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notwithstanding the foregoing, the owners of the UltraMicroscope II Imaging System may make copies solely for purposes of training personnel in the
use and servicing of the unit within their business or organization. Maximal care has been taken by Miltenyi Biotec in the preparation of this user
manual. However, Miltenyi Biotec shall not be liable for any technical or editorial errors or omissions contained herein, or for incidental or
consequential damages in connection with the furnishing, performance, or use of this document. The information in this document is provided “as is”
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UltraMicroscope II Imaging System
User manual
Original instructions
Issued: 2022-11
213443.01
Distributed by Manufactured by
Miltenyi Biotec B.V. & Co. KG
Friedrich-Ebert-Straße 68
51429 Bergisch Gladbach
Germany
Phone +49 2204 8306-0
Fax +49 2204 85197
www.miltenyibiotec.com
LaVision BioTec GmbH - a Miltenyi Biotec Company
Astastraße 14
33617 Bielefeld
Germany
Phone +49 521 260942-00
Fax +49 521 260942-10
www.lavisionbiotec.com
Read the chapter Important safety information and all
other information in this user manual before using the
instrument
Before using the UltraMicroscope II Imaging System, read the chapter Important safety information and all
other information contained in this user manual, including any safety and operating instructions. Pay special
attention to all warnings displayed on the instrument. Failure to read and follow these guidelines could lead to
improper or incorrect usage and result in damage to the instrument. Improper usage could also cause severe
personal injury, death, unpredictable results, instrument malfunction, and premature wear to components
shortening the lifetime of the instrument. Such actions may void your warranty. Keep the user manual and any
other safety and operating instructions provided with the instrument in a safe place accessible to all users for
future reference.
If you have a serious concern regarding the safe use of your instrument, contact your authorized Miltenyi
Biotec service provider or call Miltenyi Biotec Technical Support.
2
Content
1 Important safety information 5
2 Introduction 13
2.1 The UltraMicroscope II Imaging System 13
2.2 Intended use 13
2.3 Instrument description 14
2.3.1 Front view ZoomBodyconfiguration 14
2.3.2 Rear view ZoomBodyconfiguration 15
2.3.3 Front view SuperPlanconfiguration 15
2.3.4 Rear view SuperPlanconfiguration 16
2.3.5 Detail view of electronic interface 17
3 Installation 19
3.1 Components Zoom Body configuration 19
3.2 Accessories Zoom Body configuration 20
3.3 Components Super Plan configuration 20
3.4 Accessories Super Plan configuration 21
3.5 Consumables 21
3.6 Unpacking and installation 22
4 Operation 23
4.1 Switching on the instrument 23
4.2 Switching off the instrument 23
4.3 Graphical user interface 23
5 Preparing the instrument 25
5.1 Preparing the instrument 25
5.2 Setting the parameters for the alignment 26
6 Laser alignment 29
6.1 Light sheet alignment 29
6.2 Placing the alignment tool 30
3
6.3 Setting the light sheet path 30
6.4 Adjusting the lateral light sheets on the right-hand side 34
6.5 Adjusting the central light sheet on the left-hand side 34
6.6 Adjusting the lateral light sheets on the left-hand side 35
7 Setting up an experiment 37
7.1 Placing the sample 37
7.2 Capturing a single-color z-stack 37
7.2.1 Aligning the sample 37
7.2.2 Optimizing the measurement settings 39
7.2.3 Capturing an image 40
7.3 Capturing a multi-color z-stack 41
7.4 Capturing a mosaic image 42
8 Maintenance 45
8.1 Servicing 45
8.2 Cleaning the instrument 45
8.3 Cleaning the cuvette 45
9 Technical data and specifications 47
9.1 Specifications 47
9.2 EU Declaration of Conformity 49
9.3 UK Declaration of Conformity 50
10 Technical support 51
11 Legal notes 53
11.1 Limited warranty 53
11.2 Trademarks 54
4
1
IMPORTANT SAFETY INFORMATION
5
1
Important safety information
General safety instructions
This chapter provides important information for your personal safety and the correct use of the instrument.
Read and observe all instructions carefully before proceeding with the installation and use of the instrument.
Observe general safety practices in addition to this user manual.
This instrument is a Class3B laser device that requires special safety measures.
• Use this instrument only as indicated in this user manual to avoid personal injury and property damage.
• Keep this user manual in a place that is always accessible to all users.
• Follow local working area safety instructions and laboratory policies and standards for health, safety, and
prevention of accidents.
• Ensure safe working conditions and provide for sufficient light.
• Safe working is only possible if solely trained personnel uses the instrument. The training is a permanent duty
of the operator.
• Ensure that the power switch and the plug of the power cable of the instrument are easily accessible.
• To entirely disconnect the instrument from the power supply, unplug the power cable.
• Ensure that the power cable plug is close to the user.
• In case of severe accidents, damages to the instrument, or if smoke or flames appear, cut the power supply
immediately.
• If it is necessary to cut the power supply, unplug the cable from the power outlet and contact an authorized
Miltenyi Biotec service provider or Miltenyi Biotec Technical Support.
• Check the instrument before each use for visible damage.
• Do not use the instrument if it has been dropped or is damaged. Contact Miltenyi Biotec Technical Support
immediately.
Hazard levels
Signal words are used to warn against hazardous situations and property damages. The following signal words
are used in this user manual.
or WARNING! indicates a hazardous situation that, if not avoided, could result in death or serious
injury.
or CAUTION! indicates a hazardous situation that, if not avoided, could result in minor or moderate
injury. It is also used to warn against unsafe practices.
or NOTICE indicates information considered important but not hazard related (e.g. messages relating
to property damage).
Symbols
SAFETY WARNING: THE DOCUMENTATION MUST BE CONSULTED IN ALL CASES WHERE THIS SAFETY
SYMBOL IS USED ON THE INSTRUMENT
ELECTRICAL HAZARD
CRUSHING OF HANDS HAZARD
LASER RADIATION HAZARD
BIOLOGICAL HAZARD
CRUSHING HAZARD
ERGONOMIC HAZARD
TRIPPING HAZARD
SLIPPING HAZARD
WEEE (WASTE OF ELECTRICAL AND ELECTRONIC EQUIPMENT)
READ THE USER MANUAL BEFORE USING THE INSTRUMENT
ON (POWER ON)
OFF (POWER OFF)
WEIGHT
SERIAL NUMBER
TYPE NUMBER
1
IMPORTANT SAFETY INFORMATION
6
1
IMPORTANT SAFETY INFORMATION
7
MANUFACTURER
DATE OF MANUFACTURE
EUROPEAN CONFORMITY MARKING
UNITED KINGDOM CONFORMITY ASSESSED MARKING
Safety labels
Observe the hazard areas and safety symbols of the instrument.
• Keep safety labels and safety markings clean and legible.
• Inspect the safety labels and safety markings regularly and replace them if they are not legible or identifiable
from a safe viewing distance.
• Contact Miltenyi Biotec for replacement labels.
Figure 1.1: Hazard areas and safety symbols on the top side of the UltraMicroscope II Imaging System
Electrical and thermal hazards
ELECTRICAL HAZARD
Electric shock, short circuit, overheating, fire, and explosion may lead to death or serious injury.
Protective housing
The housing of the instrument reduces the risk of an electric shock and short circuits.
• Do not disassemble the instrument.
• Do not use the instrument if it has been dropped or is damaged.
Cables and power supplies
The instrument has a three-wire electrical grounding plug with a third pin for grounding. Using the instrument
with other than the supplied cables is potentially hazardous.
• Only use the included power cable.
• Do not use the instrument if the power cable is damaged.
• Only insert the plug into a grounded electrical outlet. Do not try to insert the plug into a non-grounded
electrical outlet.
• Only use the power supply provided. If you have questions about the type of power source to use, contact
the Miltenyi Biotec Technical Support or your local electricity supplier.
Liquids inside the instrument
Liquids inside the instrument can cause short circuits.
• Do not use the instrument if liquids have entered the inside of the instrument.
• Protect the instrument against spilled liquids.
• Do not allow liquids to enter the inside of the instrument.
• Clean up spilled liquids immediately.
• Unplug the instrument before cleaning it.
• Use only small amounts of cleaning agents on a soft cloth to wipe the instrument. Do not spray or pour liquid
cleaning agents onto or into the instrument.
Humid and dusty environments
A change in the environmental temperature may cause condensation inside the instrument. Humidity,
condensation, or dust inside the instrument can cause short circuits.
• Use the instrument only indoors.
• Do not use the instrument in a wet location or areas with high humidity or condensation.
• After moving the instrument from a cold environment to room temperature, wait for the instrument to
dehumidify before using it.
1
IMPORTANT SAFETY INFORMATION
8
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