BioSpherix ProCO2 120 User guide

System 1

SystemManual

version0.2

Manual

version 0.2February2007

PO Box 87

19 Demott St.

Lacona, New York 13083

PHONE 315-387-3414

FAX 315-387-3415

TOLL FREE US/CAN 800-441-3414

www.biospherix.com

1.First,adaptthegenericchamber. Inthe“AdaptingaGenericChamber”manual,refertothe:

“InstallationOverview” section, for anoverviewofhowtoinstallthegenericchamber. Alsouse

the“Adapting a Chamber for a ProCO2” section for informationanddirectionson how to

adaptthe chamber for aProCO2.

2. Now, installing the ProCO2. In the “ProCO2 Model 120” manual refer to the: “BioSpherix

Supplied Parts” and “User Supplied Parts” sections for a parts list. Also refer to the:

“Setup of Gas Supply”, “ProCO2 Front Panel Installation” and “ProCO2 Back Panel Instal-

lation” for how to make all the connections.

3. Next, familiarize yourself with the push button interface. In the “ProCO2 Model 120”

manual refer to the: “Operation”, “Program Functions Menu” and “Configuration” sections

for information regarding the push button interface.

4. Now, calibration. In the “ProCO2 Model 120” manual refer to the: “Calibration” section

for information on how to calibrate the sensor.

5. Next, tuning. In the “ProCO2 Model 120” manual refer to the: “Tuning” section for spe-

cific information on fine tuning. You shouldn’t have to use this section much, because most

tuning is done at the factory.

6. Now, operating. In the “ProCO2 Model 120” manual refer to the: “Single Setpoint Con-

trol” section for information on how to operate with the ProCO2. Also refer to the: “Setting

the Alarm Setpoint” section for directions for how to set a specific alarm setpoint.

7. Finally, maintenance issues. In all of the manuals refer to; “Maintenance” sections on

how to maintain the respective equipment.

“ProCO2” Manual v1.0 d0207

“Adapting a Generic Chamber” Manual

v0.2 d0506

This section will provide you with a list of the order in which the equip-

ment should be: installed, setup, calibrated etc. After you have com-

pleted a step refer back to this page to see what the next step is.

There will be times when the manuals with need to be used in con-

junction with one another. This list is here to help you use the equip-

ment more efficiently, you should still read all of the manuals thor-

oughly before using this list. By reading all of the manuals and then

referring to this list you will have a better understanding of how the

equipment works.

This system consists of many parts. Each

part has it’s own manual. The name of the

each individual manual and it’s version

number are as follows:

ProCO2 Model 120

Manual

version1.0Feb2007

This manual is intended to help our customers efficiently

setup and operate the equipment. We encourage not only all

installers, but also all users, to read this manual thoroughly.

Keep it handy and refer to it often. Save it for future

reference. If you have any problems or questions, please do

not hesitate to call. We are here to help.

Introduction

The ProCO2is a versatile and compact gas carbon dioxide controller for people who do

carbondioxidesensitivework. ThoughdesignedtoworkwithBioSpherixchambersthe

ProCO2worksinincubators,glove

boxes,animalcages,refrigerators,

plantgrowthchambers, andmanyother

semi-sealablechambers. Practically

anysmall-mediumenclosure can be

fitted. Evencardboardboxesand

plasticbags. Theunitworksfrom

outsideofthehostchamberbyre-

motelysensinganddisplacingthe air inside the chamber with CO2.

Thenominalrangeofcarbon dioxide concetration is 0.1-20.0%. Advanced feedback algo-

rithmshandlepracticallyanycarbondioxide gas dynamic.

Infusionratesareadjustableupto35SCFH, sufficient for

mostlabchambers. Installationis easy. Operationissimple.

Theunitmoveseasily from onehostchambertoanother.

Pleasereadandfollowthesafetyandoperations instructions

onthefollowingpages. Becareful. Anypressurizedgascan

bedangerous. Knowwhatyouaredoinganddo it safely.

ProCO2 model 120

version 1.0

Anyone who has not thoroughly read and under-

stood this manual, must never attempt to operate

the equipment.

PO Box 87

19 Demott St.

Lacona, New York 13083

PHONE 315-387-3414

FAX 315-387-3415

TOLL FREE US/CAN 800-441-3414

www.biospherix.com

Table of Contents

Safety Instructions........................................................................3

BioSpherix Supplied Parts............................................................4

User Supplied Parts.......................................................................5

Setup of Gas Supply......................................................................6

ProCO2Front Panel Installation.....................................................7

ProCO2Back Panel Installation....................................................8

Operation........................................................................................9

Program Functions Menu............................................................10

Configuration...............................................................................11

Calibration...............................................................................12-13

Tuning......................................................................................14-17

Single Setpoint Control...............................................................18

Setting the Alarm Setpoint..........................................................19

Maintenance.................................................................................20

Warranty........................................................................................21

ProCO2 model 120

version 1.0

SAFETY INSTRUCTIONS

ELECTRICAL POWER SOURCE unit should be connected to a power supply only of the

type described in the operating instructions or as marked on unit.

POWER CORD PROTECTION power supply cords should be routed so that they are not

likely to be walked on or pinched by items placed upon or against them.

ELECTRIC SHOCK do not remove cover of ProCO2due to presence of uninsulated "dan-

gerous voltage" within product's enclosure.

ELECTRIC SHOCK unit should never be used where it can fall or be pushed into water.

ELECTRIC SHOCK if modifying host chamber, be careful not to drill or cut into electrical

wires hidden behind chamber wall. Never drill or cut blindly.

NONUSE PERIODS power cord of unit should be unplugged from electrical outlet when left

unused for long period of time.

FALLING OBJECTS AND LIQUID SPLASH care should be taken so that objects do not fall

on equipment and liquids do not spill, splash, or drip onto or into unit enclosure or

power cord.

PRESSURIZED GAS secure all connections with hose clamps. Never exceed pressure

limits. Bleed all lines before disconnecting. Wear safety glasses at all times.

LOW OXYGEN ATMOSPHERES never enter a chamber which has a low oxygen atmo-

sphere because of severe danger of suffocation. Host chamber should be in well venti-

lated room. Control gas (nitrogen or other low oxygen gas) continuously leaks out of

chamber and should never be allowed to build up in room outside of chamber.

HIGH OXYGEN ATMOSPHERES never enter a chamber which has a high oxygen atmo-

sphere due to danger of oxygen toxicity. Never smoke or allow any source of fire or

spark in or around a chamber with high oxygen atmosphere. Oxygen radically promotes

combustion and can be explosive. Host chamber should always be in well ventilated

room. Oxygen continuously leaks out of chamber and should never be allowed to build

up in room outside chamber.

VENTILATION unit should be situated so that its' location or position does not interfere

with proper ventilation. Neither ProCO2nor host chamber should be in poorly ventilated

areas.

HEAT unit should be situated away from heat sources such as radiators, heat registers,

stoves, or other appliances or processes that produce heat.

CLEANING do not immerse unit in water. Do not wipe unit with wet cloth or sponge or

paper. Clean only with a dry cloth.

ProCO2 model 120

version 1.0

BioSpherix Supplied Parts

This section will familiarize you with the components that come with

the ProCO2.

12VDC power supply

Gas Fittings Infusion tubing

Calibration chamber

with tubing

Carbon dioxide gas sensor

and sensor cable

ProCO2unit

ProCO2 model 120

version 1.0

Short piece of 1/8 in. ID

tubing

User Supplied Parts5

ProCO2 model 120

version 1.0

1. Qty. 2 regulators, either a one stage regulator or a two staged regulator. Either way you

will need two gauges, one for the amount of gas in the tank and one for the gas coming out

of the tank. (2500 PSIG input 0-60 PSIG output, recommended). PSIG stands for Pounds

per Square Inch Gauge as opposed to PSIA which stands for Pounds per Square Inch

Absolute.

2. Qty. 1 compressed gas source (tank) of carbon dioxide.

3. Qty. 1 compressed gas source (tank) of carbon dioxide/oxygen mix (ratio should be 10-

90).

4. Qty. 1 compressed gas source (tank) of nitrogen (optional, see “Calibration” section for

information).

This section will list the parts that the user will need to supply in order to

operate the equipment.

Control gas must be supplied through a 1/8 in. ID tube to the back panel of the

ProCO2. If the tubing coming from the compressed gas source (tank) isn’t 1/8 in. then use

one of the provided gas fittings to adapt the tubing to 1/8 in. The pressure must be regu-

lated to 0-25 PSIG. For best results use approximately 2 PSIG in aCulture Chamber and

use 15 PSIG in an Animal Chamber. (refer to “Tuning” section under the “Power” heading

for further information). Do not connect any tubing yet, wait until told to do so in the “Cali-

bration” section and “Single Setpoint Control” section.

The amount of gas used is determined by how the chamber is used, not the ProCO2.

The ProCO2uses the least amount of gas possible, which is only what the chamber needs.

The amount of gas used is dependent on: (1) The size and leakiness of the chamber, (2)

The amount of times and how long the chamber door(s) are opened, (3) The carbon diox-

ide level being controlled.

Use a one or a two stage, 2500 PSIG input, 0-60 PSIG output regulator at the source

of the compressed gas. It is best to have two gauges so that you can monitor the amount

of gas in the compressed source and also monitor the amount passing through the output.

Never allow the pressure coming out of the compressed source to exceed 25 PSIG or

damage will occur to the ProCO2. If the regulator is not near the ProCO2 then there

should be a shutoff valve placed upstream of the unit. When taking off gas supply tube

always make sure to shut off compressed gas at the source first, then bleed the pressure

out of the line and then take off the tube from back of ProCO2.

Step-by-Step Procedure for Connecting Gas to Machine

1. Before connecting the regulator to the tank, close the regulator and make sure the tank

is also closed off.

2. Connect the regulator to the compressed nitrogen (if applicable), compressed carbon

dioxide and to the compressed carbon dioxide/oxygen mix gas sources.

3. Connect the tubing from each compressed gas source to the ProCO2when told to do so

in the calibration procedures and the operation procedures.

4. At this point you don’t have to open the regulator or turn on the gas. You will be turning

the gas on at the beginning of the calibration/single setpoint control procedures, for now

just connect the regulator.

Setup of Gas Supply

This section will describe how to setup the gas supply, for information

on how to connect the gas supply to the machine, see the calibration

sections and the “Single Setpoint Control” section.

ProCO2 model 120

version 1.0

ProCO2Front Panel Installation

The following steps will explain how to set up the ProCO2 front panel

and prepare it for installation to a chamber.

1. Placing the Unit

Place the unit on a level secure surface either on the

chamber or within seven feet of the chamber. Make sure

you can easily access the front panel.

2. Gas Connected Properly

Each time you connect a gas supply, make sure that

the gas is connected properly open the bleed valve by

turning the knob on the front of the ProCO2counterclock-

wise. If gas is heard expelling out the “BLEED” barb on

the front panel then the gas is connected properly. Once

you have confirmed then close the valve. Do this after

you have followed the procedure in the “Setup of Gas

Supply” section and are beginning either operation or

calibration.

3. Connecting Calibration Chamber

Attach the calibration chamber by connecting the

1/16 in. ID tubing to the barb labeled “BLEED” on the

front of the ProCO2, when calibrating.

4. Gas and Alarm Switches

Flip the “GAS” switch up to allow the gas to flow to

the chamber. Flip the “ALARM” switch down to enable

the alarm to sound.

ProCO2 model 120

version 1.0

ProCO2Back Panel Installation 8

ProCO2 model 120

version 1.0

3. Connecting Sensor

Plug the sensor cable into the port labeled

“SENSOR” on back of ProCO2 by twisting the sensor

cable into the port until it pushes in, then push in until

“click is heard. When removing pull the ring back and

pull cable off. Plug the other end of the sensor cable into

the sensor itself by lining up the key on the terminal with

the key on the connector of the sensor cable. Push in

and twist the ring on. The display on the front of the

ProCO2should respond.

2. Connecting Gas

Attach 1/8 in. ID tubing to hose barb labeled “IN” on

the back of the ProCO2. Making sure that the gas com-

ing in Never exceeds 25 PSIG or damage will occur to

the ProCO2. Depending on if you are calibrating or

operating will determine what type of gas you will be

using. (see “Calibration” and “Single Setpoint Control”

sections).

1. Power Supply

Plug the jack end into the port labeled “12VDC” on

back of ProCO2. Plug the 12VDC power supply into a

wall outlet. The display on the front of ProCO2should

turn on.

4. Connecting Infusion Tube

Attach 1/8 in. infusion tube to “OUT” barb on back of

ProCO2.

This section will describe how to setup the back panel of the ProCO2.

This section will give an overview of some of the different modes and

functions within the ProCO2

RESET ALARM/ERROR

MESSAGES

When the alarm or

error condition has

been cleared,

momentarily press

both upkey and

downkey at the

same time.

CHANGESETPOINT

Press and hold star

key. Press up key to

increase setpoint.

Press downkey to

decrease setpoint. If

change requires

control output, SP1

indicator will flash.

VIEWSETPOINT In work mode,

press and hold

star key. Setpoint

is shown in

alternating display.

CHANGEOPTION

Index to required function. Press and

hold star key (current option

displayed). Press up and/or down keys

to display new setting.

Release star key.

IMPORTANT:Check

new selection

(alternating with

function) before

moving to another

function or exiting

program mode.

VIEWFUNCTION/OPTION

On release of up or

down key at

function, display

alternates with the

option selected.

ENTER/EXITPROGRAMMODE

Simultaneously hold both up and down

keys for 3 seconds. You enter

program mode from

normal operating

mode at the tune

function on level 1 in

the menu. You exit

program mode at

any point in the

menu, returning to normal operating

mode. With exit, any new instructions

get entered into memory.

ENTRY TO HIDDEN LEVEL 4

At "VEr" function on

level 3, press and hold

both upkey and

downkey

simultaneously for 10

seconds. To enter level

PROGRAM MODE

All configuration, calibration,

and tuning operations take place in

program mode. You access the

programmenu,navigatethroughit,

selectappropriate options, andexit

back to work mode.

Entry into menu is always at

same point, the "tunE"function on

level1.

During programming, control

withexistingsettingsismaintained.

Newsettingsarewrittentomemory

only upon exiting program mode.

Settingsareretainedinnon-volatile

memory for years even if unit is

unpowered.

Security locks can configure

certainoptionsunadjustable. How-

ever, all functions and locked op-

tions may be viewed even when

locked.

Exit program mode from any

place in menu. Exiting program

modereturnstoworkmodewithnew

settings.

WORK MODE

Workmodeisthenormalday-

to-day operating mode. Whether

controlling or not (parked), carbon

dioxide concentration at the sensor

is continuously displayed, unless

pre-emptedbyotheroperations.

Operations in work mode are

limited to: (1) view setpoint, (2)

change setpoint, (3) reset alarm/

error message, (4) enter program

mode.

CHANGE LEVELS

Multi-level menu of functions requires

navigating from level to level.

Index down key to

level function.

Release down key

to display current

level.

Press and hold star

key while using

down key or up key

to select new level.

Release star key to

display new level.

SINGLE LEVEL NAVIGATION

Press up

or down

key once

to move to

the next

function.

Hold up or down keys to automatically

index through the functions. Always

index down to get to level function.

OPERATION 9

ProCO2model120

version1.0

Table of contents

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