Biosan Multi Bio 3D User manual

Multi Bio 3D

Programmable Shaker

for version

V.2AW

Operating Manual

Certificate

Medical–Biological

Research & Technologies

Page 2

Contents

1. Safety Precautions

2. General Information

3. Getting Started

4. Operation

5. Program setting

6. Specifications

7. Maintenance

8. Warranty and Claims

9. Declaration of Conformity

Page 3

1. Safety precautions

The following symbol means:

Caution! Make sure you have fully read and understood the present Manual

before using the equipment. Please pay special attention to

sections marked by this symbol.

GENERAL SAFETY

·Use only as specified in the operating manual provided.

·The unit should be saved from shocks or falling.

·The unit must be stored and transported in a horizontal position (see package

label).

·After transportation or storage keep the unit under room temperature for 2-3hrs

before connecting it to the electric circuit.

·Use only cleaning and decontamination methods recommended by the

manufacturer.

·Do not make modifications to the design of the unit.

ELECTRICAL SAFETY

·Connect only to the external power supply unit with voltage corresponding to that

on the serial number label. Use only the external power supply unit provided with

this product.

·Ensure that external power supply unit is easily accessible during use.

·Disconnect the unit from electric circuit before moving. Disconnect the external

power supply unit from power socket to turn off the unit.

·If liquid penetrates into the unit, disconnect it from the external power supply unit

and have it checked by a repair and maintenance technician.

DURING OPERATION

·Do not impede the platform motion.

·Do not operate the unit in environments with aggressive or explosive chemical

mixtures.

·Do not operate the unit if it is faulty or has been installed incorrectly.

Page 4

·Do not use outside laboratory rooms.

·Do not place a load exceeding the maximum load value mentioned in the

Specifications section of this Manual.

BIOLOGICAL SAFETY

·It is the user's responsibility to carry out appropriate decontamination if

hazardous material is spilt on or penetrates penetrates into the equipment.

2. General Information

Multi Bio 3D Programmable Shaker is designed for laboratory studies in

the areas of biochemistry, immunochemistry, microbiology and genetic

engineering. It can be used for:

·gel washing, blot-hybridization, electrophoretic analysis of proteins and

nucleic acids;

·preliminary preparation of blood samples before analyses to prevent blood

coagulation;

·tissue and cell lysis: different mixing modes will facilitate getting best

extraction results;

·cell cultivation in trays and other flat vessels during the preparation of

growth media in Petri dishes (microbial antibiotic resistance

investigations).

Multi Bio 3D Programmable Shaker provides 3 types of motion:

3D Orbital Shaking

Combination of:

•3D shaking;

•orbital shaking

- with adjustable speed from 1 to 100 rpm.

- with 7° pitch.

Page 5

3D Reciprocating Shaking

Combination of:

•3D shaking;

•reciprocating shaking with adjustable

turning angle from 0 to 360° (increment 30°):

- with 7° pitch;

- with adjustable speed from 1 to 100 rpm.

3D Vibro Shaking

Intensive mixing. Combination of:

•3D shaking;

•vibro shaking with adjustable turning

angle from 0 to 5°(iscrement 1°):

- with 7° pitch.

These 3 motions are consecutively combined into a cycle and can be

used

– separately;

– in combinations by two;

– all three in one cycle (Fig. 1).

3D Reciprocating and 3D Vibro motion types can be replaced with a

pause.

Each cycle can be repeated up to 125 times or run continuously.

Fig.1. Innovative mixing cycle

Page 6

3. Getting started

3.1. Unpacking.

Remove packing materials carefully and retain them for future shipment

or storage of the unit.

3.2. Complete set. Package contents:

Standard set

-Multi Bio 3D Programmable Shaker ........................................1 piece

-Bio PP-4S platform w...............................................................1 piece

-external power supply unit........................................................1 piece

-Operating Manual; Certificate ...................................................1 copy

Optional accessories

-TP-26 adapter for tubes u..................................................on request

-PDM optional dimmpled mat v...........................................on request

3.3. Set up:

-Place the unit on the horizontal even working surface;

-Plug the external power supply into the 12 V socket at the rear side of the

unit.

3.4. Install the platform by inserting the pins on the underside of the platform

into the holes on the supporting platform on the unit.

TP-26 PDM Standard detachable Bio PP-4S

platform with rubber mat

Page 7

4. Operation

4.1. Connect the external power supply to electric circuit.

4.2. Place samples on the unit platform.

4.3. Set the required program and number of cycle repetitions (p.5 Program

setting).

4.4. Press the Run/Stop key (Fig.2/11) to start the program.

4.5. The platform motion will begin and the corresponding indication

(operation mode - RUN (Fig.2/10), cycle countdown (Fig.2/9) and the

changing time values) will be shown on the display.

4.6. The unit will stop after performing the set number of cycles (flashing

indication STOP on the display) and give a sound signal about the end of

operation (press the Run\Stop key to stop the signal).

4.7. Press the Run/Stop key to repeat the preset program.

4.8. The unit can be stopped at any time during operation before the set

number of cycles is performed if necessary by pressing the Run/Stop

key. Pressing the Run/Stop key again will start the program from the

beginning (cycles countdown will be restarted).

4.9. If the number of cycles is not set (cycle number indicator (Fig.2/9) shows

zero), pressing the Run\Stop key will start continuous operation of the

unit until the Run\Stop key is pressed again.

4.10. Unplug the external power supply unit from electric circuit to turn off the

unit.

Fig.2. Front panel

Orbital Reciprocal Vibro Cycle

Run/Stop

Select

Mode

Time

30 180 5 00

15 30 5 RUN

1 2 3 4 5 6 7 8 9 10

Page 8

5. Program setting

The program consists of cycles. Each cycle includes three different types

of platform motion (3D orbital, 3D reciprocating and 3D vibro) set one after

another with the duration from 0 to 250 seconds for 3D orbital and 3D

reciprocal motion types and from 0 to 5 seconds for 3D vibro motion.

The set cycle can be repeated from 1 to 125 times or non-stop.

5.1. Press the Select key (Fig.2/3) to choose the parameter to change (the

active parameter is blinking).

5.2. Use the p and q keys (Fig.2/8) to set the necessary value (pressing the

key down for more than 2 sec will make the values change quickly).

5.3. The program can also be changed during operation: microprocessor

automatically will enter the last changes into the working memory as the

working program when the new cycle begins.

5.4. It is necessary to set speed (Fig.2/1), turning angle (Fig.2/4, Fig.2/6), time

for each motion type (Fig.2/2, Fig.2/5, and Fig.2/7) and number of cycle

repetitions (Fig.2/9).

5.5. If the time for a motion is set to zero, this type of motion will be skipped in

the cycle.

5.6. It is possible to set a pause instead of 3D reciprocal (0-250 sec) or 3D

vibro (0-5 sec) motion. To set a pause set the turning angle of 3D

reciprocal or 3D vibro motion to zero and set the time for this motion,

which will be the time of pause duration. (During the operation the

platform will not move in this mode but the time will be counted down.)

Orbital

Reciprocal

V ibro

OFF

Pause

OFF

Pause

OFF

Pause

Page 9

5.7. Table shows different cycle variants.

5.8. Further examples illustrate program setting for four different cycle

variants.

5.8.1. 3D orbital

Most popular Sunflower kind of motion.

Set the speed (A) (1 - 100 rpm) and time (B) (1 - 250 sec) of 3D orbital

motion. Turn off 3D reciprocal motion by setting time of 3D reciprocal motion to

zero (C) (OFF).Turn off 3D-Vibro type motion by setting time of 3D vibro

motion to zero (D) (OFF). See fig.3.

Fig.4 shows 3D orbital motion run in cycles.

On On

OFF

On On

Pause

OFF

Pause

OFF

Pause

OFF

Orbital

Reciprocal

V ibro

Time, s

Cycle 1 Cycle 2 …

Fig.4.

100 180 5 00

250 OFF OFF RUN

Orbital Reciprocal Vibro Cycle

Time (sec.)

rpm

Fig.3.

5.8.2. 3D orbital + 3D reciprocal + 3D vibro

Set the speed (A) (1 - 100 rpm) and time (B) (1 - 250 sec) of 3D orbital

motion. Set the angle (C) (0 - 360º) and time (D) (1 - 250 sec) for 3D reciprocal

motion. (It is performed at the same speed as the orbital motion.) Set the

turning angle (E) (0-5º) and time (F) (1 - 5 sec) for 3D vibro type motion. See

fig.5.

Page 10

Fig.5.

Time (sec.)

100 180oo

500

15 15 5 RUN

Crpm

Time, s

Cycle 1 Cycle 2 …

Orbital Reciprocal Vibro Cycle

Fig.6.

Fig.6 shows 3D orbital, 3D reciprocal and 3D vibro motions run one after

another in cycles.

5.8.3. 3D orbital + 3D reciprocal + pause

Set the speed (A) (1 - 100 rpm) and time (B) (1 - 250 sec) of 3D orbital

motion. Set the turning angle (C) (0 - 360º) and time (D) (1 - 250 sec) for 3D

reciprocal motion. Set the angle of (E) 3D vibro type motion to zero. Set the

time for 3D vibro motion (F) (1 - 5 sec) - this is the time of pause duration. See

fig.7.

Fig.8 shows 3D orbital and 3D reciprocal motions and pause run one after

another in cycles.

5.8.4. 3D vibro + pause

Turn off 3D orbital motion by setting time of 3D orbital motion to zero (A)

(OFF). Set the angle of 3D reciprocal type motion to zero (B). Set the time for

3D reciprocal motion (C) (1 - 250 sec) - this is the time of pause duration. Set

the turning angle (D) (0 - 5º) and time (E) (1 - 5 sec) for 3D vibro type motion.

See fig.9.

Time (sec.)

100 180oo

10 10 5

rpm

Time, s

Cycle 1 Cycle 2 …

Fig.8.

Fig.7.

Orbital Reciprocal Vibro Cycle

RUN

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