Biosan FTA-1 User manual

Contents

1. About this edition of user instructions.......................................................................3

2. Safety precautions ...................................................................................................4

3. General information..................................................................................................5

4. Getting started..........................................................................................................6

5. Operation .................................................................................................................9

6. Specifications.........................................................................................................10

7. Care and maintenance...........................................................................................12

8. Warranty.................................................................................................................14

9. EU Declaration of conformity..................................................................................15

1. About this edition of user instructions

The current edition of the user manual applies to the following models and versions

of aspirators with trap flasks:

FTA-1 version V.4AW

FTA-2i version V.1A01

2. Safety precautions

Caution! Make sure you have fully read and understood the present Manual

before using the equipment. Please pay special attention to sections

marked by this symbol.

GENERAL SAFETY

The protection provided can be ineffective if the operation of the appliance does not

comply with the manufacturer's requirements.

Save the unit from shocks and falling.

Store and transport the unit at ambient temperatures between -20°C and +60°C and

maximum relative humidity of 80%.

After transportation or storage and before connecting it to the electric circuit, keep the

unit under room temperature for 2-3 hrs.

Use only original parts and accessories, provided by manufacturer for this product.

Before using any cleaning or decontamination methods except those recommended

by the manufacturer, check with the manufacturer that the proposed method will not

damage the equipment.

Do not make modifications in design of the unit.

ELECTRICAL SAFETY

Connect only to the mains with voltage corresponding to that on the serial number

label.

Use only the external power supply provided with this product.

Ensure that the power plug is easily accessible during use.

Disconnect the unit from the mains before moving.

If liquid penetrates into the unit, disconnect it from the mains and have it checked by

a repair and maintenance technician.

Do not operate the unit in premises where condensation can form. Operating condi-

tions of the unit are defined in the Specifications section.

DURING OPERATION

Do not operate the unit in environments with aggressive or explosive chemical mix-

tures. Please contact manufacturer for possible operation of the unit in specific at-

mospheres.

Do not operate the unit if it is faulty or has been installed incorrectly.

Do not use outside laboratory rooms.

Do not use the unit for aggressive organic compounds, chlorinated solvents and

strong conjugated acids.

BIOLOGICAL SAFETY

It is the user’s responsibility to carry out appropriate decontamination if hazardous

material is spilt on or penetrates into the equipment.

Empty the trapping flask when the liquid level reaches maximum. Take necessary

precautions utilizing waste liquid in accordance with general laboratory standards.

3. General information

FTA-1 & FTA-2i aspirators with trap flask have been designed for liquid removal from

micro-test tubes (e.g. for supernatant removal during DNA/RNA extraction), ELISA plates

(e.g. performing several repeated well washing cycles), cell culture flasks (cell media ex-

change and washing during cell growth) and other vessels during various laboratory tech-

niques.

Model FTA-1 has a 1 L glass trap flask and is designed to be a low-cost solution.

Aspiration is possible with a single standard 200 μL tip and the 8-tipped MA-8 adapter.

Model FTA-2i comes with a 2 L polypropylene trap flask and a liquid overflow sensor

that stops collected material from damaging the filter. Aspiration is possible with single 200

and 1000 μL tip using the MA-U adapter or with the handheld vacuum controller HAS-1 with

different attachments such as 8-channel adapter with stainless steel tips, 8-channel adapter

for 200 μl tips, single channel adapter for 200 μl tips and others.

Microbiologic suction filter eliminates risks of contamination caused by bacteria, vi-

ruses, and infected particles from samples. Such contamination can be distributed by suction

pump or central vacuum units. Microbiological suction filter is hydrophobic and has very high

contamination removal bacterial efficiency –up to 99.99% of particles bigger than 0.027

micrometres (e.g. Hepatitis A, B and C, HIV, etc.).

In order to prolong performance and efficiency of device it is not allowed to use ag-

gressive organic compounds (e.g. phenol) and strong conjugated acids (e.g. guanidine) for

aspiration. Please, contact manufacturer in case of any questions regarding operation of

device.

4. Getting started

4.1. Unpacking. Remove packing materials carefully and retain them for future shipment

or storage of the unit. Examine the unit carefully for any damage incurred during

transit. The warranty does not cover in-transit damage.Warranty covers only the units

transported in the original package.

4.2. Complete set. Package contents:

4.2.1. FTA-1:

- FTA-1, aspirator with trap flask ........................................................................1 pce.

- Hydrophobic filter.............................................................................................1 pce.

- Aspiration tips, 200 µL....................................................................................50 pcs.

- Holder tubes (1.5 ml) .....................................................................................10 pcs.

- Spare hydrophobic filter ...................................................................................1 pce.

- External power supply .....................................................................................1 pce.

- Operating manual, declaration of conformity ..................................................1 copy

- MA-8, 8-channel aspiration tip with holder................................................on request

4.2.2. FTA-2i:

- FTA-2i, aspirator with flask trap........................................................................1 pce.

- MA-U, universal adapter ..................................................................................1 pce.

- Hydrophobic filter.............................................................................................1 pce.

- Aspiration tips, 200 µL....................................................................................50 pcs.

- Aspiration tips, 1000 µL..................................................................................10 pcs.

- Holder tubes (2 ml) ..........................................................................................5 pcs.

- Spare hydrophobic filter ...................................................................................1 pce.

- External power supply .....................................................................................1 pce.

- Operating manual, declaration of conformity ..................................................1 copy

- MA-8, 8-channel aspiration tip with holder................................................on request

- HAS-1, hand operator set with SH-6 stand...............................................on request

Figure 1. Assembled FTA-1 Figure 2. Installation of MA-8 aspiration tip

Figure 3. FTA-2i front view Figure 4. FTA-2i lid with sensor and tubing

Figure 5. MA-U, universal adapter

Figure 6. FTA-2i filter setup Figure 7. FTA-2i rear panel

4.3. FTA-1 setup.

- Place the unit on horizontal even working surface.

- Install the trapping flask (fig. 1/5) on the unit.

- Assemble the lid, aspiration tubing and filter set (fig. 1/2). Attach aspiration tip

(fig. 1/6) to the tubing.

Caution! Position the filter with the IN marking pointing towards the unit (fig. 1/2).

- Screw on the lid with the filter set and aspiration tubing to trap flask. Ensure that the

filter set is tightly inserted.

- Connect the filter tubing to the pump (fig. 1/1) located at the top of the base behind

the trap flask, by turning the fitting clockwise.

- Place the microtubes for aspiration tip into the holder (fig. 1/4).

- Connect the external power supply unit into the socket at the rear side of the unit and

position the unit for an easy access to the external power supply and the power switch.

4.3.1. Optional MA-8 aspiration tip installation. Remove microtubes and aspiration tip

from the holder (fig. 1/4). Place MA-8 storing holder (fig. 2/1) into the holder. Remove

aspiration tip (fig. 1/6) from the aspiration tubing and connect the MA-8 aspiration tip

(fig. 2/2). Store the aspiration tip in the new holder.

4.4. FTA-2i setup.

- Place the unit on horizontal even working surface.

- Install the trapping flask (fig. 3/2) on the unit.

- Screwon thelid with the sensor needles and valves for tubing (fig. 3/1) to trapping flask.

- Assemble the filter set (figure 6) and connect it to the port on the rear panel of the

unit (fig. 7/1).

Caution! PositionthefilterwiththeINmarkingpointing awayfromtheunit(fig. 6/1).

- Attach the fitting (fig. 4/1) of the tubing to the filter (fig. 4/2) to the valve with white

ring on the lid.

- Ensure that the filter set is tightly fixed in the slot (fig. 7/1).

- Assemble the MA-U aspiration tip set (figure 5).

- Attach the fitting (fig. 4/3) of the tubing for liquids (fig. 4/4 and 5/3) to the valve with

the blue ring on the lid.

- Connect the sensor cable (fig. 4/5) to the lid of the unit and to the port on the rear

panel of the unit (fig. 5/3).

- Place the microtubes for aspiration tip into the holder (fig. 3/3).

- Connect the external power supply unit into the socket at the rear side of the unit (fig.

7/4) and position the unit for an easy access to the external power supply and the

power switch.

4.4.1. Optional MA-8 aspiration tip installation. Remove microtubes and aspiration tip

from the holder (fig. 3/3). Remove the aspiration tip (fig. 5/1) from the MA-U adapter

and connect the MA-8 aspiration tip (fig. 8/1).

4.4.2. Optional HAS-1 hand operator installation. Unscrew the MA-U adapter from the

aspiration tube (fig. 5/3) and connect the HAS-1 hand operator (fig. 8/) by screwing

it to the fitting.

5. Operation

5.1. Connect the external power supply to the mains.

5.2. Turn the Power switch (fig. 1/3 or 7/2) to the position I(on). The pump starts working.

5.3. (For model FTA-2i) Regulate the aspiration intensity with the regulator knob on the

front panel of the unit (fig. 3/4). Clockwise turning direction increases power, counter

clockwise –decreases. Current intensity is indicated by a light ring around the regu-

lator knob.

5.4. Touch the surface of the liquid with the aspiration tip (fig. 1/6, 5/1 or MA-8) to remove

the liquid.

Caution! During operation, do not allow the collected liquid to exceed the max-

imum level. Empty the trapping flask when the liquid level reaches

maximum allowed level, according to 5.7.

Note. In model FTA-2i, when collected liquid exceeds the maximum level,

the sensor turns off the pump and produces sound and light warning

signals.

5.5. (For model FTA-2i) When using the optional HAS-1 hand regulator, touch the liquid

with the aspiration tip, then press and hold the blue button on the regulator to collect.

5.6. After finishing the operation, switch off the Power switch and disconnect the external

power supply from the mains.

5.7. Emptying the trapping flask. Turn off the unit. Remove the tubings and cables from

the lid. Remove the closed flask from its place. Unscrew the lid and empty the trap-

ping flask. Screw the lid back on and return the trapping flask on the unit. Replace

the tubings and the cables.

Caution! Take necessary precautions in utilizing waste liquid in accordance

with general laboratory standards. For information regarding auto-

claving procedures, see 7.4.

6. Specifications

The unit is designed for operation in cold rooms, incubators (except CO2incubators)

and closed laboratory rooms at ambient temperature from +4°C to +40°C in a non-condens-

ing atmosphere and maximum relative humidity 80% for temperatures up to 31°C decreasing

linearly to 50% relative humidity at 40°C.

Biosan is committed to a continuous programme of improvement and reserves the

right to alter design and specifications of the equipment without additional notice.

FTA-1

FTA-2i

Aspiration speed

up to 1.7 mL/s

up to 27.8 mL/s

Vacuum regulation

None

Yes

Working vacuum pressure

-500 mbar

-200 … -800 mbar

Overflow protection sensor

None

Yes, invasive

Overflow protection actions

None

Pump stop, sound and light signals

Trapping flask

1 L, glass

2 L, polypropylene (PP)

Air filtration

Hydrophobic microbiologic air filter 2200/021

Filter pore diameter

0.027 μm

Input current

DC 12 V, 300 mA

DC 12 V, 1 A

Power consumption

3.6 W

10.8 W

External power supply

input AC 100-240 V, 50/60 Hz; output DC 12 V

Dimensions

160x210x340 mm

185x290x390 mm

Weight2

1.7 kg

1.85 kg











Figure 8. Optional accessories and replacement parts

Eliminates risk of contamination from the trapping flask with bacteria, viruses and infected particles

Accurate within ±10 %

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