BestScope BS-7000B User manual

Inverted Fluorescent Microscope

BS-7000B

Instruction Manual

This manual is written for Inverted Fluorescence Microscope BS-7000B. For safety and keeping

the best performance, making you familiar with the instrument entirely, it is strongly

recommended that you read this manual carefully before using the microscope.

BestScope International Limited

User Notices................................................................................................................................ 2

1. Components Name................................................................................................................ 4

2. Installation................................................................................................................................5

2.1 Installing diagram.......................................................................................................... 5

2.2 Installment steps............................................................................................................6

3. Adjustment and operation....................................................................................................11

3.1 Lamp adjustment for fluorescence observation......................................................11

3.2 Reflected observing illumination adjustment.......................................................... 17

4. Microscope photography and video.................................................................................. 19

4.1 Microscope video........................................................................................................ 19

4.2 Microscope photography............................................................................................20

5. Outfit....................................................................................................................................... 21

5.1 Specification.................................................................................................................21

5.2 Objective Specifications............................................................................................. 22

6. Troubleshooting.................................................................................................................... 22

BestScope International Limited

User Notices

Safety Note

1. The epi-fluorescent attachment is a precise instrument. Open the box carefully, and avoid

dropping the accessories to ground and causing damage to them.

2. Do keep the instrument out of direct sunlight, high temperature or humidity, dusty and vibrations.

3. Make sure that the lamp(mercury lamp) is installed correctly and all cords are connected firmly.

4. Do not open the lamp housing while it is turned on, lamp housing parts are extremely hot and

may cause burns if touched. Users can open the lamp housing at least 10 minutes after it has

been turned off.

5. Always be sure to ground (earth) the equipment.

6. Verify that the voltage and the frequency of the AC mains outlet match the setting of the voltage

switch and the frequency switch on the rear of the power supply unit.

7. Always use the power cord provided and make sure that the main switch is moved to “O”(OFF)

before connecting the power cord plug to the wall outlet.

8. To prevent any hazard, always turn the main switch on the power supply unit to “O” (OFF), unplug

the power cord plug from the mains outlet before replacing the lamp or the fuse, and wait for at

least 10 minutes before replacing the lamp. (Be sure to use a GCQ-100 mercury lamp.)

9. To prevent obstruction of the air flow, it is important to leave enough space around and above the

lamp housing.

Safety Symbol

Symbol

Meaning

The surface is very hot, not touch by your hands

Before using, please read the instruction carefully, improper operation will result in

bodily injure or instruction malfunction.

The main switch on

The main switch off

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Maintenance and Storage

1. Clean all glass components by wiping gently with gauze. To remove fingerprints or oil smudges,

wipe with gauze slightly moistened with a mixture of ether (70%) and alcohol (30%).

Since solvents such as ether and alcohol are highly flammable, they must be handled

carefully. Be sure to keep these chemicals away from open flames or potential sources of

electrical sparks. For example, electrical equipment that is being switched on or off. Also

remember to always use these chemicals only in a well-ventilated room.

2. Do not attempt to use organic solvents to clean the non-optical component of the equipment. To

clean these, use a lint-free, soft cloth lightly moistened with a diluted neutral detergent.

3. Do not disassemble any part of the power supply unit as malfunction or damage may occur.

4. In order not to impair the safety of the equipment, replace the lamp when the counter of NFP-1

indicates “100.00” hours. To prevent any hazard, always turn the main switch on the power

supply unit to “O” (OFF), unplug the power cord plug from the mains outlet, and wait for at least

10 minutes before replacing the lamp. High-pressure gas is sealed within the mercury lamp.

Thus, if it is continued to be used after its service life expectancy, the glass tube may deform and

may sometimes rupture.

BestScope International Limited

1. Components Name

Trinocular tube head Phase contrast slider Transmitted illumination

Reflected fluorescence units

Condenser

Illumination bracket

Eyepiec

Stage inserted plate

Stage

Body

Nosepiece

Objectives

。4×

。10×

。20×

。40×

Fluorescence power supplier

Protection barrier

B excitation, G excitation

Lamp house

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2. Installation

2.1 Installing diagram

The following Figure shows the installation sequence of the components. The number in the Figure

shows the installation steps.

*Before installing, be sure every components is clean, do not score any parts or glass surface.

*Keep well with the supplied hexagon wrench. When changing the components, you will need it again.

①

②

④

③

⑤

⑥

⑦

⑧

Condenser

Phase contrast slider

Transmitted Lamp house

Eyepiece

Trinocular head

Objectives

Glass stage

Power supply unit

Mechanical ruler

Microscope Body

Auxiliary Stage

⑨

⑩

⑾

⑿

Lever

Protection barrier

Mercury lamp

house

BestScope International Limited

2.2 Installment steps

2.2.1 Installing trinocular head(Fig 1)

Loosen the setscrew ③on the microscope body②

and insert the trinocular Viewing Head ①into the body

correctly, tight the setscrew ③.

2.2.2 Installing eyepiece(Fig 2)

Insert the eyepiece④into tube until they are against.

2.2.3 Installing condenser set(Fig3)

Install the condenser into the right direction(Fig3).

Fig 1

Fig 2

②

①

③

④

Fig 3

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Fig 4

2.2.4 Installing lamp house (Fig4, Fig5)

Insert the plug A on the lamp house into hole A of

power cord, then insert the plug B on the lamp house

into the B hole of the condenser till there are against.

(like Fig 6).

2.2.5 Installing phase contrast plate (Fig6,

Fig7)

1. Keep the slider face(the surface which has number

10-20) up towards. Each light ring or hole has its own

located position, so you need to move them until you

hear the “clicked” to ensure the ring or the hole reach

the center of the light path(show as Fig 7).

2. Turn the aperture diaphragm lever ①to adjust

aperture. Turn the diaphragm to a Max. aperture when

do phase contrast observation.

● The phase contrast ring has been centered

beforehand, so it needn’t to be adjusted when in the

use. If the ring is not in the center, you could adjust

by the centering bolt.

● The 10X/20X phase contrast ring is worked with the

10X, 20X phase contrast objective, while the opening

hole is used for bright field objective.

Fig 5

Fig 6

Notices

Environment Requirement:

1. Temperature: 0℃～40℃, max humidity: 85%.

2. High temperature and moisture will damage

instrument and affect performance.

3. Keep the instrument away from the dust environment,

and take the dust cover when no using.

4. Put the instrument on table without vibration.

Fig 7

①

Replace lamp

1. Turn the switch to off position when need to

replace the lamp. Pull out the lamp house and

then the lamp after it is cool down completely.

2. Insert the new lamp softly to prevent damage.

3. Please do not touch the lamp by bare hands, in

order not to reduce lamp working life or cause

explosion. Clean the fingerprint by wiping slightly

with soft cloth which is moistened with ethyl

alcohol.

BestScope International Limited

Fig 8

③

②

①

2.2.6 Installing the objective (Fig8, Fig9)

1. Turning the coarse focusing knob①like the figure shows

till the nosepiece get to its lowest position.

★For ensuring the safety of the instrument during

transportation, the nosepiece has been adjusted to

the lowest position and the tension adjustment

collar②has been adjusted to a appropriate tight

tension while leaving the factory.

2. Screw the lowest magnification objective on to the

nosepiece, then turn the nosepiece clockwise, mount

other objectives according the magnification sequence

from low to high.

◎Mount objective like this way will make the change of

magnification to be very easy in using.

◎The objectives also can be installed through the stage

opening.

★Clean the objective regularly, the objective used in the

inverted microscope is very sensitive to dust.

★Do cover all the unused holes on nosepiece with dust

caps③, to prevent the dust and contamination entering

inside.

★When operating, use the low magnification objective (4X

or 10X) to search and focus the specimen at first, then

use the higher magnification objectives if necessary.

★When change the objective, slowly turning the nosepiece

until you hear “clicked”, that means the objective enter

into the right position—center of the light path.

2.2.7 Mounting the Mercury lamp (Fig10, Fig 11)

1．Loosen the lamp socket clamping screw ①, and remove

the lamp socket. (Fig.10)

2．After removing the foam backstop②, securely insert

the + pole (the thick head) of the mercury lamp③to the

lower terminal first and then the – pole(the thin head) to

the upper terminal, then tighten the two clamping

screws④.

3．Close the lamp socket with lamp into the original position

and tighten the socket clamping screw①.

Fig 10

Fig 9

①

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Fig 13

●Be sure to use a 100W HBO ultrahigh pressure spherical

mercury lamp.

●Be sure to mount positive pole(the wide head) before the

other , or the damage to the lamp may occur.

●Never subject the lamp to excessive force when mounting the

Mercury lamp.

●Be careful and avoid leaving fingerprints or dirt on the mercury

lamp. Attached stain may cause distortion in glass which

could result in a ruptured lamp. If stained, wipe it a way gently

with clean gauze.

★To prevent any hazard, always turn the main switch on the

power supply unit to “O” (OFF), unplug the power cord from

the mains outlet, and wait for at least 10 minutes before

replacing the lamp.

2.2.8 Assembly of the Fluorescent Attachment ,

Cable and Cord Connections (Fig 12, 13)

1. Mount the lamp housing into the other end of the fluorescent

attachment and fix it with two screws④.

2．Plug the connector ⑤from the lamp housing securely into the

connector on the power supply unit and make sure the cord

is correctly connected. (Make sure that the main switch⑥of

the power supply is set to “O” (OFF) before connecting

cables)

3. Connect the power cord which along with the microscope into

connector on the power supply unit and connect the other

end of the cord to the mains jack.

● Verify the voltage and the frequency of the AC mains outlet

match the microscope pre-set voltage and frequency, which

is marked on the rear of the power supply units. Improper

input voltage and frequency may degrade lamp performance,

or in the worst case (although very rare), cause the lamp to

explode.

● It is better to use the power cord provided by us and the same

type power cord should be used if you lose or damage the old

one.

2.2.9 Fuse Replacement (Fig 12, 13)

1. Set the main switch to “O” (OFF) and unplug the power cord

before replacing fuses.

2. Using a flat-blade screwdriver, remove each of the fuses

holders⑦by tuning it anti-clockwise and pulling out.

3 Replace both fuses with new ones.

● Always use the designated fuses (2pcs 8A). Make sure the

voltage of the fuse match the voltage of the AC mains outlet.

② ③

Fig 12

Fig 11

④

⑥

⑤

⑦

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