BestScope BS-2025M User manual

To ensure the safety and obtain satisfactory performance, please study this operation

instruction thoroughly before your operation.

Biological Microscope

Model: BS-2025M/B/T

Instruction Manual

BestScope International Limited

Contents

1. Purpose .....................................................................................................2

2. Name of Components...............................................................................2

3. Specifications ............................................................................................3

4. Installation ................................................................................................5

5. Operation ..................................................................................................6

6. Maintenance ...........................................................................................11

7. Troubleshooting......................................................................................14

8. Outfits .....................................................................................................16

BestScope International Limited

1. Purpose

BS-2025 series biological microscopes are designed for teaching, clinical verify.

This series microscopes with original style, steady structure, convenient

operation and clear image is suitable for observing various biological specimens,

and mostly applied in school, college, hospital and family.

2. Name of Components

Fig. 1 BS-2025M Monocular Microscope

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Fig. 2 BS-2025B Binocular Microscope

3. Specifications

3.1 Total magnifications

Objective

Total Magnifications

Eyepiece

10X

40X

100X

10X/18mm

40X

100X

400X

1000X

16X/11mm

64X

160X

640X

1600X

3.2 Objectives

Working Distance

Objective

Numerical

Aperture

(N.A.)

Objective Field

(Field Number

ф18)

Resolving

Power

Achromatic

Plan

0.10

4.5mm

2.8µm

13mm

16mm

10X

0.25

1.8mm

1.1µm

6.3mm

2mm

40X (S)

0.65

0.45mm

0.42µm

0.44mm

0.66mm

100X

(Oil) (S)

1.25

0.18mm

0.22µm

0.3mm

0.42mm

3.3 The other specification

3.3.1 Mechanical tube length: 160mm

3.3.2 Conjugate distance: 195mm

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3.3.3 Head: Monocular, Inclined 30°, Rotable 360°, Eyepiece can be fixed,

Anti-fungal systems.

Seidentopf binocular head 30°, Rotable 360°,

Interpupillary adjustable distance is 55-75mm,

Diopter adjustable range±5, Anti-fungal systems.

3.3.4 Nosepiece: Quadplex nosepiece

3.3.5 Mechanical stage: Size 142mm×134mm

X-Y travel 50mm×70mm

3.3.6 Focusing systems: Coaxial coarse and fine focusing knobs, Coarse stroke

22mm, Fine division 2µm, Condenser up-down range

25mm

3.3.7 Condenser: Abbe condenser, N.A. 1.25, Adjustable aperture,

Aperture center is adjustable.

3.3.8 Mirror: Mirror diameterф52mm (optional)

3.3.9 Filter: Built in blue filer, ф38.5mm

3.3.10 Anti-fungal systems: Eyepiece and objective is anti-fungal, anti-fungal

system built in head.

3.3.11 Electric components: Input voltage AC85-265V, 50/60Hz

Output voltage DC1.2-6V

6V/20W halogen lamp

Rotation potentiometer with power switch

Fuse 2A ф5×20

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4. Installation

BS-2025 series microscopes are packed as integrate, and it is convenient for

installation.

Open the package carton, and insert eyepiece into the eyepiece tube of the

head (eyepiece for monocular microscope has been inserted before packing). If

objectives don’t be fixed, please take objectives out from objectives packing

box, and drive them into the holes of nosepiece orderly and tightly according to

the magnification.

Take out the oil bottle and dust cover, and place it properly for convenient use.

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5. Operation

5.1 Electric components

Make sure the supply voltage meets the instrument’s requirement, then plug in

it. Rotation potentiometer with power switch used by the instrument is for

protecting lamp.

5.2 Placing specimen (Shown in Fig.3)

Please place specimen on the stage, and the face with cover glass should be up,

then clip it with clamp. Clipping specimen should be careful in order to avoid

spoiling glass. Place specimen on the stage levelly, and make it into optical path

by adjusting vertical moving knob and horizontal moving knob.

5.3 Condenser (Shown in Fig.3)

5.3.1 Condenser up-down

Turn the condenser up-down knob to adjust

the distance between condenser front and

the specimen, and change the equal

Mirror

Bracket

Mirror Base

Fig.4 Mirror

ClampHandleSpecimen Clamp Condenser

Vertical

MovingKnob

Horizontal

MovingKnob

Handleof

IrisAperture

Condenser

Up- downKnob

Fig.3 StageAndCondenser

Diopter Ring

Eyepiece

Interpupillary

DistanceScale

Fig.5 Interpupillary Distance

Adjustment And Diopter Ring

EyepieceTube

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illumination to obtain the best brightness.

5.3.2 Iris aperture adjustment

Stir the handle of iris aperture of condenser to adjust size of iris aperture for

adjusting the contrast of the specimen.

5.4 Interpupillary distance and diopter adjustment (Shown in Fig.5)

5.4.1 Interpupillar distance is different for everyone, so interpupillary distance

should be adjusted before using binocular microscope. Please take the two

eyepiece tubes to turn until the bright rings observed by two eyes are in

superposition entirely.

▲BS-2025M monocular microscope don’t need to be adjusted.

5.4.2 Diopter adjustment

As focusing for binocular, user should observe right eyepiece with right eye,

and make the right eyepiece clear by focusing adjustment, then observe the

left eyepiece, at the same time, adjust the diopter ring of the left eyepiece tube

to make the image of left eyepiece clear as same as the right eyepiece.

5.5 Focusing adjustment

Usually 10X objective should be used for focusing adjustment first. Take 10X

objective into bright path, then observe the specimen with right eyepiece by

right eye and turn coarse focusing knob to find the image, then turn slowly fine

focusing knob to make the image clear.

All objectives of our microscope are precisely adjusted, and approximate clear

image should be found with the other objectives after getting the clear image

with 10X objective. If the image isn’t enough clear with the other objectives,

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please turn fine focusing knob slightly to find the satisfactory image.

▲Don’t turn left and right coarse and fine focusing knobs with different

direction with power at the same time, if so, the focusing system will be

damaged.

▲Don’t directly pull objective to turn nosepiece when user changes the

different objectives, if so, optical

quality of microscope possibly be

affected. The right way is to take the

tooth-like part of the nosepiece to

turn it, and make the objective into

correct position and into the bright

path.

5.6 Usage of the tension adjustment ring (Shown in Fig.6)

The tension adjustment ring can adjust the tension of the coarse and fine

focusing unit to prevent the stage from sliding down automatically and to

improve the comfort of operation. Rotation clockwise makes tension decrease,

and by contraries, rotation counterclockwise makes tension increase.

5.7 Usage of immersion oil objective

The 100X objective of BS-2025 series microscopes can be used for observation

even if without immersion oil. However, adding moderate immersion oil

between the front lens of 100X objective and the cover glass of specimen can

make the image clearer. Please pay attention that air bubble and impurity can’t

be in the immersion oil, otherwise, the image would be affected.

Fig.6 Coarse A F Fnd ine ocusing

Left Coarse

Left Fine

Right Fine

Right Coarse

Tension

FocusingKnob

Adjustment Ring

FocusingKnob

KnobsAnd Tension Adjustment Ring

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First of all, take 40X objective which has been focused well out from bright path,

then take 100X objective into bright path. At this time, nosepiece or stage

moving knob should be turned slightly, and also slightly turn fine focusing knob

to clear away the air bubble of immersion oil, otherwise, the air bubble would

affect the image badly.

▲After immersion oil used, the oil of specimen and the microscope surface

should be soon cleaned by absorbent cotton, lens paper, gauze or soft cotton

cloth with moderate mixture of pure industrial alcohol and ether (proportion

1:4)

▲Standard thickness 0.17mm cover glass should be chosen when high times

objective used, and thickness error should be within 0.01mm, otherwise, image

definition would be affected.

5.8 Mirror (Shown in Fig.4)

Mirror of BS-2025 series microscopes is optional, and it is used when electrical

power isn’t supplied.

Mirror doesn’t be suggested as illumination source when 100X objective used.

Take light collector down from base, then take mirror on base. Turn mirror to

make reflecting face to the light source, and make reflecting face and horizontal

face as 45°approximately to make the light to specimen as possible. The mirror has

two sides, one is a plane mirror, and the other one is a concave one. Concave

mirror is suitable for large-area outside light source, such as bright sky.

5.9 Points of attention after using

5.9.1 Power of microscope should be turned off after using, and the plug

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