BestScope BS-2036F2B(LED) User manual

Fluorescent Biological Microscope

BS-2036F2B(LED)/F2T(LED)

Instruction Manual

BS-2036F2B(LED) BS-2036F2T(LED)

This manual is written for BS-2036F2B(LED)/F2T(LED) Series LED fluorescent biological microscope.

To ensure the safety, obtain optimum performance and to familiarize you fully with the use of this

microscope, it is recommended strongly that you study this manual thoroughly before using the

microscope and retain this manual in an easily accessible place near the work desk for future

reference.

 BestScope International Limited 
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Contents 
Microscope Body Part.....................................................................................................３
1. Pars Name ............................................................................................................３
2. Specifications .......................................................................................................４
3. Installation ...........................................................................................................５
4. Operation .............................................................................................................５
5. Image Collection ..................................................................................................６
6. Maintenance........................................................................................................７
Fluorescent Part..............................................................................................................８
1.Applications ..........................................................................................................８
2.Principle ................................................................................................................８
3.Instruction.............................................................................................................９
4.Installation ............................................................................................................９
5. Fluorescence Device Operation .......................................................................１１ 
Trouble shooting .........................................................................................................１３ 
Outfits .........................................................................................................................１４ 
 
 
 
 
 
 
 

BestScope International Limited

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Attentions！！

1. Purpose.

This series microscopes are used only for microscopic observation, not available for

other purpose, otherwise result in equipment damage.

2. Disassembly only by the professionals.

The microscope has been adjusted before shipping, Unprofessional-person should not

disassemble and remove any other parts. Disassemble and remove any other parts

will result in equipment damage.

If you have any questions, please contact with manufacturer or local distributor.

3. Note the input voltage if correspond.

This instrument designed for wide input voltage (100V~240V, 50/60HZ), applicable to

most areas. If the supply voltage exceeds this range, the instrument will be seriously

damaged.

4. Prevent Burns and Fire.

When using power equipment, bulbs and collecting mirror and other nearby parts of

the set will rise sharply in temperature until it reaches a thermal equilibrium state.

Pay attention to anti-hot logo, they should be careful not be burn when in use.

Alcohol, gasoline, paper and other flammable materials can’t near the lamp in case

of fire.

5. Notes on Replacing the Bulb.

Replacement should be based on the identity of the instrument using the same

specifications of the bulb, otherwise it may cause equipment damage.

The power supply must be cut off before bulb replacement, the bulb must be cooled

off completely before proceeding! !

6. Carry.

Power must be cut off before moving. Be careful not to crush your finger when

placed.

This instrument is a precision instrument, and it should be handle with care, severe

shock can cause serious damage to related parts.

7. Installation.

Please refer to the installation instruction in order to avoid to damage the

instrument.

8. Operation Environment.

The required available environment for using of the equipment:

Indoor temperature: 0 °C ~ 40 °C , maximum relative humidity: 85%

High temperature or high humidity may cause mildew, fog or dew of the optical

components, and make the instrument not work.

9. Packing Waste Disposal

For the protection of the environment, please properly handle the microscope

packing waste or send to salvage station (such as: cardboard, foam, etc. )!

10. Statement

Our company reserves the right to improve product design and outfits.

BestScope International Limited

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Microscope Body Part

BS-2036F2B(LED)/F2T(LED) series LED fluorescent biological microscopes are designed for college

teaching, medical and clinical identification. The microscope with modern design, steady structure,

convenient operation and clear image is suitable for observing various biological specimens, they

are mostly applied in colleges and hospitals.

1. Pars Name

①

②

C_③

④

D_⑤

E_⑥

⑦

⑧

①

Eyepiece

②

Objective

③

Clamp

④

Mechanical Stage

⑤

Condenser

⑥

Light Collector

⑦

Main Body

⑧

Seidentopf

Binocular Head

(Trinocular Head)

⑨{

Power Input

Fuse

⑨

Diopter Adjustment Ring

Nosepiece

Clamp Handle

Handle of Iris Aperture Diaphragm

Field Diaphragm Ring

Body Handle

Head Fixing Screw

Mechanical Stage Moving Knob

Right Coarse & Fine Focusing Knobs

Potentiometer

Power Switch

Condenser Focusing Knob

Tension Adjustment Ring

Left Coarse & Fine Focusing Knobs

BestScope International Limited

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2. Specifications

2.1 Total magnifications

Objective

Total Magnifications

Eyepiece

10X

20X

(Optional)

40X

100X

10X

40X

100X

200X

400X

1000X

16X

64X

160X

320X

640X

1600X

1.2 Objectives

Infinite Plan Objectives are optional.

Infinite Plan

Objectives

Numerical

Aperture (N.A.)

Objective

Field

Resolution

(μm)

Working

Distance(mm)

0.10

5mm

3.35

12.1

10X

0.25

2mm

1.34

4.64

20X (Optional)

0.40

1mm

0.83

2.41

40X (S)

0.65

0.5mm

0.50

0.65

60X (S) (Optional)

0.80

0.33mm

0.41

0.33

100X (Oil) (S)

1.25

0.2mm

0.26

0.12

1.3 The other specification

2.3.1 Mechanical tube length: 180mm

2.3.2 Conjugate distance: Infinity

2.3.3 Head: Seidentopf Binocular or Trinocular, Inclined 30°, Rotatable 360°, Anti-fungal

systems. Interpupillary Adjustable Distance Is 48-75mm, Diopter adjustable range ±5.

2.3.4 Nosepiece: Quadplex nosepiece

2.3.5 Mechanical stage: Size 145mm×140mm, X-Y travel 76mm×52mm

2.3.6 Focusing systems: Coaxial Coarse and Fine Focusing Knobs, Coarse stroke 26mm,

Fine division 2µm, Condenser up-down range 22mm

2.3.7 Condenser: Abbe condenser, N.A. 1.25, Adjustable aperture, Aperture center can be

adjustable.

2.3.8 Illumination: Non-spherical System

2.3.9 Filter: Built in blue filer

2.3.10 Electric components: Input voltage AC85-265V/LED

Output voltage DC1.2-6V/LED,

Rotation potentiometer with power switch, Fuse 2A ф5×20

BestScope International Limited

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Condenser

screw

Condenser

installing ring

Condenser

3. Installation

Please clean the operation desk before installation. Put out the microscope of the carton and put it

on the desk.

Make sure the supply voltage meets the instrument’s requirement and the power switch is off.

Installation Instruction Fig.:

1.Turn the binocular (trinocular) head to working position;

2.Put out the dust cover of the eyepiece tube;

3.Insert into the eyepiece;

4.Install Abbe Condenser.

3.1 Eyepiece Tube:

Loose the Head Fixing Screw G, turn the tube to observing

position, then tighten the Screw G.

3.2 Put out the eyepiece dust cover.

3.3 Eyepiece

Put out the eyepiece of the carton, and insert it into the

tube. Please don’t touch the lens of the eyepiece by hand.

3.4 Condenser

Put out the condenser of the carton, then turn

Condenser Focusing Knob M to lower condenser

installing ring. Loose the condenser screw, then

install the condenser, and make the condenser

graduation face to the front, Tighten the condenser

screw, and higher the condenser installing ring to

top. Please don’t touch the lens by hand.

3.5 Power

Put in power, open Switch, and turn Potentiometer.

4. Operation

BestScope International Limited

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4.1 Bright field operation process instruction

5. Image Collection

5.1 Installing

Connect the C-mount with CCD camera or connect camera with camera adapter, then connect it

with c-mount, finally put it into microscope.

5.2 Using

First get a clear image from eyepiece, then put out lever on the side of trinocular head, and collect

image with camera. Clear image should be in screen. Adjust B14 fine focusing knobs to get it clear if

image is not clear.

5) Adjust condenser aperture

diaphragm

Adjust condenser ⑤(condenser focusing

screw M) and aperture (Aperture diaphragm

lever D)

Observing

1) Turn power switch,

adjust light

Turn on Switch L, and turn Potentiometer K.

2) Put the sample

Put the sample on stage ④, (open the clamp

C, and put the sample into it), turn stage knob

H to put the sample into optical path.

3) Put 10X objective into

optical path and focus

Turn Nosepiece B, put 10×objective into

optical path. Turn Coarse & Fine Focusing

Knobs I and N to focus.

4) Adjust interpupillary

distance and diopter

Adjust head ⑧to make two fields into one;

adjust diopter adjuster A to make diopter for

two eyes same.

6) Adjust and center field

diaphragm

Adjust field diaphragm

(field diaphragm ring E)

Put the objective into

light path, and focus

BestScope International Limited

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6. Maintenance

6.1 Clean microscope

6.1.1 Don’t touch the lens with hand, Dust on lens should be cleaned by soft brush or absorbent

cotton or cleaned by absorbent cotton, lens paper with the mixture of alcohol and ether

(proportion 1:4).

6.1.2 Alcohol and ether all are burnt early, please take them away from fire. Be careful for turn on

and off power.

6.1.3 Don’t clean painted metal and galvanizing metal with organic solvent such as alcohol, ether or

the mixture of the both. Silicon cloth or soft cleaning preparation is suggested to clean it.

6.1.4 Plastic should be cleaned by soft cloth with clear water.

6.2 Environment of using and placing

6.2.1 Microscope should be used and placed in a cool, dry, non-dust, non-shake and non-corrosive

gases environment.

6.2.2 Microscope should be used in environment of indoor temperature 0°-40°C and maximum

relative humidity 85%.

6.2.3 Removing equipment is suggested to be installed when microscope used in heavy humidity

area to avoid fungus and mist damage instrument.

6.2.4 Please pay attention to prevent microscope from violent shake and vibration in application

and in carrying. Don’t drag it on the surface of worktable to avoid damage to microscope and

worktable.

6.3 Replacement of bulb

6.3.1 Turn off power, and pull out plug.

6.3.2 Wait the bulb become cool.

▲Please be sure that the bulb is cool, then follow by

the nest operations.

6.3.3 Lay aside the microscope reliably, unscrew the

knurled thumb screw of the lamp housing cover on the

underside of base.

6.3.4 Pull over the lamp housing cover.

6.3.5 Pull out the bulb should be replaced, hold a new

bulb with silk cloth to avoid fingerprint and dust affect bulb brightness and service life, and insert

fully the contact pins into the bulb socket.

6.3.6 Close the lamp housing cover, and screw the knurled thumb screw.

▲After working for above 10 hours continuously, better

cut off the microscope about 30 minutes.

6.4 Replacement of fuse

6.4.1 Cut off power of microscope, pull out the plug.

6.4.2 Unscrew fuse cap in the back of base, take out old

fuse.

6.4.3 Replace a new fuse, then screw the fuse cap.

BestScope International Limited

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Fluorescent Part

1.Applications

Some objects can emit a ray which wavelength is longer than that of the excitation light when

irradiated. This ray is called fluorescence, and observers can study the objectives through

fluorescence microscope using the phenomenon.

Fluorescence microscope has wide applications in basic theory research and clinical diagnosis

about medicine, biology, as well as analysis and test in industry, agriculture, stockbreeding,

criminal investigation, legal medical appraise, environmental protection etc.

2.Principle

The device consisting of

Epi-fluorescence illuminator,

5W LED lamp, fluorescence

objectives is combined with

microscope to make up

fluorescence microscope. The

device is designed and

manufactured with

Epi-excitation principle

and standard provided with

two group excitation filters

system: blue (B), green (G).

The light emitted from the

lamp is converted to the

excitation light (e.g.blue light)

with specified wavelength by

going through the excitation filter, then passes through dichroic prism and objectives (the

objective plays role of condenser) to irradiate vertically the object. The object is excitated and

emits fluorescence with specified wavelength (e.g. green and yellow) and make image passing

through objectives, dichroic prism and eyepieces. The light (including excitation light) without

fluorescence wavelength is reflected or absorbed by dichroic prism and barrier filter, and can

not reach the view system. Therefore, what can be seen in the view field is the bright

fluorescence image against the dark background.

Fluorescence Device Diagram

Fluorescence

module

Condenser

prism

LED

lamp

Barrier filter

Dichroic

prism

Excitation filter

Eyepiece

Objective

Specimen

BestScope International Limited

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3.Instruction

The fluorescence microscope is building-block structure, it includes main body, fluorescence

conversion device, fluorescence excitation modules, fluorescence light source lamphouse and

objective etc. Fluorescence device with 2 position can install two excitation modules and bright

field observation position. Standard outfits: FL2-LED and special LED device for single. In

addition, other excitation modules can be used as per user’s requirements.

FL2-LED two-groups fluorescence device with LED illumination, it has excellent feature, without

waiting startup time for lamp, low voltage, no fever on lamp house etc. Standard equipped with

B,G two group fluorescence modules. Single LED fluorescence device is designed for single

module, customize for especial requirement.

4.Installation

Two-Groups LED Fluorescence Device Installation Marking Graphs

LED Fluorescence Device Installation Marking Graphs

Ordinary LED Power Box

Lithium power source

This manual suits for next models

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