BestScope BS-2010BD User manual

Bestscope International Limited

Digital Binocular Microscope

BS-2010BD

Instruction Manual

This manual is written for digital binocular microscope BS-2010BD. To ensure the safety, obtain optimum

performance and to familiarize the use of this microscope, it is recommended strongly that you study this

manual thoroughly before using the microscope and retain this manual in an easily accessible place near

the work desk for future reference.

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1. Brief introduction

Digital binocular microscope BS-2010BD is widely used in biological, pathological, bacteriological

fields for purposes such as observation, research, academic demonstration, clinic, medical inspection. It

features more comfortable and reliable operation with modern design and advanced technology.

Brightness adjusting wheel

Main switch

Fine focusing knob

Coarse focusing knob

Limit bolt

Collector

Condenser

Stage

Objective

Eyepiece

Digital Binocular head

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2. Main Specifications

1. Mechanical tube: 160mm

2. Objectives (achromatic)：

Magnification

Numerical

Aperture

Focal Length

f (mm)

Working

distance (mm)

0.10

31.05

37.5

10X

0.25

17.13

7.316

40X

0.65

4.65

0.632

100X

1.25

2.906

0.198

3. Eyepiece

Category

Magnification

Focal Length f(mm)

Field of view（mm）

Plan Eyepiece

10X

24.95

Ø 18

4. Total Magnification

Eyepiece

10X

Objective

10X

40X

100X

Total

40X

100X

400X

1000X

5. Objective conjugate distance: 195mm

6. Stage: double layer stage 125mmX115mm

7. Coarse focusing range: 6.5mm

8. Condenser: Abbe Condenser, NA=1.2 with iris diaphragm

9. Diameter of filter: Ø28mm

10. Illuminator: LED Lamp, brightness adjustable

3. Outfit

Name

Specifications

Standard outfit

Body

Stand

Double-layer mechanical stage

Base (LED lamp)

Viewing Head

Binocular digital head

Condenser

NA1.2 Abbe Condenser

Nosepiece

Quadruple

Illuminator

LED lamp

Eyepiece

10X Plan Eyepiece

Objectives

10X

40X

100X

Immersion oil

5ml

AC Adapter

USA/European/UK plug type

User Manual

English version

Package

Foam box and carton

Bestscope International Limited

4. Structure Feature

a) Eyepiece, objective and nosepiece

10X plan eyepieces combined with 4X, 10X, 40X, 100X achromatic objectives can give the

microscope a large and clear field of view. The nosepiece has a precise frame which can locate

objectives accurately and stably. The digital binocular head can turn 360 degree freely with

comforts and conveniences.

b) Curved arm and coarse and fine focusing frame

The C-type molding of the curved arm is sprightly. The coarse focusing frame adopts dovetail

guide and move smoothly with upper limit device.

c) Base

The base, with built-in illuminator, is the foundation of whole microscope which adopts

streamline circular in consonance with the curved arm.

d) Stage

Use double-layer mechanical stage, the slide is fixed with slide clip.

e) Illumination system

The illumination system consists of illuminator, iris diaphragm and condenser.

Abbe condenser can be raised and lowered by simple operation.

5. Assembly

A) Environmental Requirement：

1. Ambient temperature: 5°C to 40°C(41°F to 104°F), Maximum R.H: 85%.

2. High Temperature will result in a mildewing, dew and even ruinous instrument.

3. Avoid placing the instrument in a dusty environment. When ending your microscope operation,

please cover it with the dust cover.

4. Lay the microscope in a plan and stable position, please.

B) Input voltage check:

Check the input voltage: be sure the input voltage which signed in the back of the microscope

is consistent with the power supply voltage, or it will bring a serious damage to the instrument.

C) Eyepiece:

Insert the eyepiece into the eyepiece tube.

D) Objective:

Adjusting the coarse focus knob until the mechanical stage reaches its lower limit position.

Screw the lowest magnification objective into the nosepiece from the left or the right side,

then revolve the nosepiece clockwise and mount other objectives in the sequence of low to high

magnification

E) Condenser:

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The condenser had been installed in the microscope before leaving factory.

F) Collector:

The collector had been installed and located before leaving factory. The screw-mounted

collector can be taken down by anticlockwise turning.

H) Filter:

Mount the filter on the iris diaphragm bracket under the condenser if necessary.

6. Operation

1. Insert the eyepiece into the eyepiece tube and revolve the objectives into the nosepiece in

sequence of magnification.

2. Place the specimen in the center of the stage, keeping the cover slip upward, and then nip it

with the specimen holder.

3. Search the image by lower magnification objective, turn the longitudinal and transversal

adjustment knobs which on the mechanical ruler to move the specimen onto the center of the

field of view. Then replace with higher magnification objective, turn the fine focusing knob to

sharpen the image.

4. When using 100X oil objective, add the immersion oil between the objective and specimen.

5. You should be aware of the cleaning procedures to be used after the use of oil. Wipe oil from

the oil immersion objective, and from any other parts in need of cleaning. Clean lenses immediately

with xylene after use. Leaving oil on the lens may lead oil to seep behind and harden on the inside of

the lens.

★To obtain a bright and clear image, it is import to adjust the illumination system. The condenser

position and the iris diaphragm size should match with objectives of different magnifications. Turn the

condenser focus knob to shift the condenser. It needs to raise the condenser when using the high

magnification objective, and to decline when using the low magnification one.

★The aperture diaphragm is used to adjust the numerical aperture instead of brightness.

Generally, setting the size of the aperture diaphragm at 70%～80% of the numerical aperture, you can

obtain a clear image with enough contrast.

★For observing a batch of specimen of the same type, use the limit bolt to locate general

position of the stage to simplify operation. In this way, the specimen can come into focus rapidly just

by turning the coarse focusing knob.

Note: specimens of the same type means they are same in the thickness of slide, cover slip and

specimen slice.

7. Maintenance

1. Carefully open the box, avoid the accessories, like lens, dropping to ground and being damaged.

2. All the lenses have been well checked and adjusted. It is forbidden to disassemble them by user

self.

3. The nosepiece and coarse/fine focus unit have a compact and precise frame, so please don’t

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disassemble them as possibly as you can.

4. Keep the instrument clean, wipe dust regularly, apply a little non-corrosive grease in the sliding

parts and be attention to avoid contaminating the optical elements especially.

5. Place the instrument in a cool, dry position. When not using the microscope, keep it covered with

a dust helmet. Make sure the lamp socket is cool before covering the microscope.

8. Troubleshooting

Under certain conditions, performance of this unit may be adversely affected by factors other

than defects. If a problem occurs, please review the following list and take remedial action as needed.

If you cannot solve the problem after checking the entire list, please contact your local distributor for

assistance.

1) Optical part

Problem

Cause

Solution

The edge of the field of

view has shadow or

the brightness is

asymmetry

The nosepiece is not in the located

position (objective is not in the center of

the optical path)

Turn objective to the right

position (the optical light

center)

The image of the filament is not in the

center

Center it

The surface of the lens is moldy or has

contaminant (include condenser,

objective, eyepiece and collector)

Clean it up

Find dust and stain in

the field of view

There are stains on the lens (include

condenser, objective, eyepiece and

collector)

Clean it up

There are stains on the specimen

Clean it up

The position of the condenser is too low

Raise it to proper position

The image is

defocused (low

resolution \ contrast)

There is no coverslip on the specimen

Add coverslip

The coverslip is too thick or too thin

Use the standard coverslip

( 0.17mm )

The specimen is placed inversely

Reverse it back

There was oil on the dry objective (easily

happened in 40X objective)

Clean it up

There are stains on the lens (including

condenser, objective, eyepiece and

collector)

Clean it up

The oil objective didn’t immersion oil

Use immersion oil

There was bleb in the oil

Eliminate the bleb

Use an unsuitable oil

Change to the specified

one

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The size of the aperture diaphragm is too

large

Minify it

The size of the aperture diaphragm is too

small

Open it up

The position of the condenser is too low

Adjust the position

One side of the image

is dark

The condenser is not in the center of the

field of view\the condenser inclines

Re-install the condenser

The nosepiece is not in the right position

Turning it until it reaches

the “clicked” position

The specimen is floating

Fix it

The image shift during

focusing

The specimen slips on the stage

Fix it

The nosepiece is not in the right position

Turn it to the “clicked”

position

2) Mechanical part

TROUBLE

CAUSE

SOLUTION

The image can focus

when using high

magnification objective

The specimen is placed inversely

The coverslip is too thick

Turn inversely

Use the standard coverslip

(0.17 mm)

The objective and the

specimen touch when

change objectives from

low magnification to

higher magnification

The specimen is placed inversely

The coverslip is too thick

Turn inversely

Use the standard coverslip

(0.17 mm)

The specimen is not

easy to move

The specimen holder is not fixed

Fix it

The eyes are too tired

The brightness is not suitable

Adjust the voltage of the

lamp

3) Electrical Part

TROUBLE

CAUSE

SOLUTION

The lamp can’t light

when turning on the

switch

No power supply

Check the connection of

the power cord

The LED lamp is burnt out

Replace it

The lamp burns out

suddenly

The voltage is too high

Use the specified AC

adapter, if the problem is

not solved, contact with

the service department

Bestscope International Limited

The brightness is not

enough

Use a substandard AC adapter

Use the specified AC

adapter

The voltage is too low

Add the voltage

9．The software Scopeimage 9.0

1) Installation

1. Link the digital Binocular head to a PC by the USB cable, the system will find the new device, and then

run the driver under the CD, please follow the clue to finish the installation.

Attention: please link the camera to a PC before running the driver installation; and when there

comes out warning dialog, just click ‘Continue Anyway’, as the pictures show below:

2. Install the software, follow the clue to finish the installation, after the installation, there will be a

shortcut icon on the Desktop and the Start Menu.

3. Please install the video codec ‘wmv9VCMsetup.exe’, it can compress the video files when recording,

and greatly reduce the size of the video files. If you can not do record, please check whether you have

installed the video codec already.

4. Double click the shortcut icon on the desktop to preview the images, please insure the camera has

linked with the PC, and the driver has been installed rightly, or there will come out an error hint:

Cannot preview successfully. Please check your camera.

Attention:

★Please install all the three setup files in the CD, otherwise the camera may can not work normally.

★USB2.0 is a mandatory, not optional. This camera cannot work on the computers with USB1.1 ports.

In order to ensure the stability of the connection with the USB2.0 interfaces, please insert the plug

into those interfaces in the back panel the mainframe.

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★Please use the same USB2.0 port every time. Using on a different USB2.0 port requires reinstalling or

updating the driver again to make the camera work rightly. If not, it will influence the performance of the

camera, and cause the live image displays drag, for there is another system driver, and the system set it as

default driver.

★Support operation system: Windows2000/XP/Vista/Win 7/Win 8/Win 10, 32bit & 64bit operation

system.

★Please unplug the USB cable from the computer immediately after using. It will shorten the lifetime of

the camera, sometimes may cause repair service if the camera is plugged in the computer all the time

and you do not shut down the computer for a long time.

2) Operation

ScopeImage 9.0 is image-processing software professionally designed for digital microscope. It allows you

to view, capture, edit, record, zoom, measure, and process microscope images. The main interface of the

software is as follows:

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Besides image windows, many other windows or user interface elements reside within the main window

of ScopeImage 9.0. These include menu, toolbars, status bar and control windows, providing additional

information about the images or control of the camera.

ScopeImage 9.0 mainly divides into two parts operation, one is for the video operation, and the other one

is for the image operation;

Video operation includes video preview, video control, color adjustment, video capture, ect.

Image operation includes image processing, image flip, image calibration and measurement, ect.

ScopeImage 9.0 is image-processing software professionally designed for digital microscope. It allows you

to view, capture, edit, record, zoom, measure, and process microscope images. Specific features included

are as follows:

1）Active image preview, freezing preview, fit to window preview, real preview, full screen

preview… various preview modes make you preview the active images with great ease.

2）Convenient color adjustment of the active image, containing brightness, contrast, saturation,

RGB value, white balance and so on, very convenient to adjust the color during previewing.

3）Special design color database, we can associate the parameters of the color to the database, and

can store or delete the color scheme.

4）Various image measurement tools, including line, angle, rectangle and circle, can move, delete

or set the color of the objects. And you can also burn all the objects into the image for further

using.

5）Basic image operations like other similar software are included in the Image menu. You can

adjust the image at ease.

6）Image flip function, include horizontal flip and vertical flip of the active image, and rotate 90

degree, 180 degree, 270 degree or any angle degree of the captured image.

7）Image Zoom: Contain image zoom in, zoom out, 1:1.

Attention:

When launching the software, there will be several seconds displays as black preview, please don’t worry,

it is normal, because the camera needs time to do some pretreatment, to ensure the stable working of

the camera. The same reason for the recording, when you begin to record, it will display as black preview

for several seconds, and when the images display normal, the recording begins.

Detail information about how to operate the software, please refer to the user manual in the CD.

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