Agilent Technologies AssayMAP Bravo User manual



AssayMAP

Bravo Platform

Installation Guide

Original Instructions

Notices

No part of this manual may be reproduced

in any form or by any means (including

electronic storage and retrieval or

translation into a foreign language) without

prior agreement and written consent from

Agilent Technologies, Inc. as governed by

United States and international copyright

laws.

Installation Guide Part Number

G5571-90001

Edition

Revision B, March 2018

Contact Information

Agilent Technologies Inc.

Automation Solutions

5301 Stevens Creek Blvd.

Santa Clara, CA 95051

USA

Technical Support: 1.800.979.4811 (US) or

+1.408.345.8011 (rest of world)

[email protected]om

Customer Service: 800-227-9770 (US and

Canada)

By country: http://www.agilent.com/en-

us/contact-us/page

Documentation feedback:

[email protected]

Web:

http://www.agilent.com/en-us/products/

automation-solutions

Acknowledgements

Microsoft® and Windows® are either

registered trademarks or trademarks of the

Microsoft Corporation in the United States

and other countries.

Warranty

The material contained in this docu-

ment is provided “as is,” and is sub-

ject to being changed, without notice,

in future editions. Further, to the max-

imum extent permitted by applicable

law, Agilent disclaims all warranties,

either express or implied, with regard

to this manual and any information

contained herein, including but not

limited to the implied warranties of

merchantability and fitness for a par-

ticular purpose. Agilent shall not be

liable for errors or for incidental or

consequential damages in connection

with the furnishing, use, or perfor-

mance of this document or of any

information contained herein. Should

Agilent and the user have a separate

written agreement with warranty

terms covering the material in this

document that conflict with these

terms, the warranty terms in the sep-

arate agreement shall control.

Technology Licenses

The hardware and/or software described in

this document are furnished under a

license and may be used or copied only in

accordance with the terms of such license.

Restricted Rights Legend

If software is for use in the performance of

a U.S. Government prime contract or sub-

contract, Software is delivered and

licensed as “Commercial computer soft-

ware” as defined in DFAR 252.227-7014

(June 1995), or as a “commercial item” as

defined in FAR 2.101(a) or as “Restricted

computer software” as defined in FAR

52.227-19 (June 1987) or any equivalent

agency regulation or contract clause. Use,

duplication or disclosure of Software is

subject to Agilent Technologies’ standard

commercial license terms, and non-DOD

Departments and Agencies of the U.S. Gov-

ernment will receive no greater than

Restricted Rights as defined in FAR 52.227-

19(c)(1-2) (June 1987). U.S. Government

users will receive no greater than Limited

Rights as defined in FAR 52.227-14

(June1987) or DFAR 252.227-7015 (b)(2)

(November 1995), as applicable in any

technical data.

Safety Notices

A WARNING notice denotes a

hazard. It calls attention to an

operating procedure, practice, or the

like that, if not correctly performed or

adhered to, could result in personal

injury or death. Do not proceed

beyond a WARNING notice until the

indicated conditions are fully

understood and met.

A CAUTION notice denotes a hazard. It

calls attention to an operating procedure,

practice, or the like that, if not correctly

performed or adhered to, could result in

damage to the product or loss of important

data. Do not proceed beyond a CAUTION

notice until the indicated conditions are

fully understood and met.

iiiAssayMAP Bravo Platform Installation Guide

Contents

1. Before you start. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1

About this guide . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2

Related guides and where to find them. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2

Safety information. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4

1. Installing the platform . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1

Overview of the hardware components. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2

Computer requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3

Installation workflow for standard configuration. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4

Installing the Bravo risers . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5

About installing the AssayMAP head . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8

Installing the 96AM Wash Station and Pump Module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10

Installing the standard platepads . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20

Installing the Peltier Thermal Station . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21

Installing and connecting the Orbital Shaking Station . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26

2. Setting up the computer and software . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31

Setting up the computer and installing the software . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32

Turning on the platform and setting up the VWorks software . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37

Verifying the profile settings and configuration. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39

About verifying the deck configuration in the profile . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43

Verifying the Autofill Station configuration (96AM Wash Station). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45

Verifying the Peltier Thermal Station configuration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47

Verifying the Orbital Shaking Station configuration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49

Initializing the profile that you are verifying . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51

Testing the accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52

3. Setting the teachpoints . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 57

Teaching part 1: Setting teachpoints in AssayMAP 1 profile. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58

Teaching part 2: Adjusting teachpoints for accessories. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62

Teaching part 3: Updating the other profiles based on AssayMAP 1 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 67

Checking and adjusting the gripper offset . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 72

4. Verifying the installation and setup. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 79

Verification workflow . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 80

About installing the Bravo pendant, Light Curtain, and shields . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 80

Opening the Workbench and accessing the utilities. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 82

Running the Startup utility. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 83

Testing the safety equipment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 84

Washing the syringes to prepare for first time use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 86

Performing a mock run . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 90

Running the Syringe Test utility . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 97

Running the Shutdown utility . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 98

Contents

iv AssayMAP Bravo Platform Installation Guide

Workflow for setting teachpoints in each profile manually. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 100

Adjusting the teachpoint for the Bravo Plate Riser. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 101

Adjusting the teachpoint for the red PCR plate insert . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 103

AssayMAP Bravo Platform

Installation Guide



Before you start

•“About this guide” on page 2

•“Related guides and where to find them” on page 2

•“Service documentation” on page 4

•“Safety information” on page 4

Before you start

About this guide

2AssayMAP Bravo Platform Installation Guide

About this guide

Intent

This installation guide is intended for use by qualified and authorized Agilent

Technologies service personnel who possess an in- depth service background

and have received training in the installation of the AssayMAP Bravo Platform.

What this guide covers

This guide describes the following software versions:

•Protein Sample Prep Workbench v3.0

•VWorks Automation Control v13.1.1

•Bravo Diagnostics v19.1.1

What is new in this revision

and

Related guides and where to find them

Checklists

You can find the following checklists on the Agilent AssayMAP Bravo Platform

product page at https://www.agilent.com/en/products/automation- solutions/

protein- sample- preparation/assaymap- bravo- platform

•Site Preparation Checklist. Specifies the site requirements that must be

met before installing the AssayMAP Bravo Platform.

•Installation Checklist. Lists the tasks to be performed to install the

AssayMAP Bravo Platform.

Feature and description See…

Revised the startup procedure to match

instructions in the Protein Sample Prep

Workbench v3.0.

“Running the Startup utility” on

page 83

Reorganized the workflow for testing the

safety equipment.

“Testing the safety equipment” on

page 84

Revised the syringe wash procedure to

specify 60 wash cycles.

“Washing the syringes to prepare

for first time use” on page 86

Before you start

Related guides and where to find them

AssayMAP Bravo Platform Installation Guide

User guides

The following table lists the user guides and where to find them.

To access the VWorks Knowledge Base, do one of the following:

•From within VWorks software, select Help > Knowledge Base or press F1.

•From the Windows desktop, select Start > Agilent Technologies >

VWorks Knowledge Base.

•Go to the online VWorks Knowledge Base at www.agilent.com/chem/askb

and click the link for PDF user guides.

Guide Description Where to find the guide

Bravo Platform Unpacking

Guide

Unpacking instructions for the Bravo

Platform

Attached to shipping

container and in VWorks

Knowledge Base

Bravo Platform Safety and

Installation Guide

Safety guidelines, site specifications,

and installation procedures

Included in the shipping

container and in VWorks

Knowledge Base

Bravo Platform User Guide Setting up, operating, maintaining,

and troubleshooting the Bravo

Platform

VWorks Knowledge Base and

Protein Sample Prep

Workbench

Pump Module User Guide Setting up the Pump Module and the

autofilling reservoirs and wash

stations

VWorks Knowledge Base and

Protein Sample Prep

Workbench

AssayMAP Bravo Platform

Error Recovery Guide

Error messages, and how to handle

them

VWorks Knowledge Base and

Protein Sample Prep

Workbench

AssayMAP Bravo Platform

Labware Reference Guide

Labware photo reference and

descriptions

VWorks Knowledge Base and

Protein Sample Prep

Workbench

AssayMAP Bravo Syringe

Replacement User Guide

Replacing a defective syringe in the

Bravo 96AM Head

VWorks Knowledge Base and

Protein Sample Prep

Workbench

AssayMAP 96AM Wash

Station Maintenance Guide

Replacing chimneys in the 96AM

Wash Station

VWorks Knowledge Base

VWorks Automation Control

Setup Guide

Defining labware and labware classes,

liquid classes, and pipetting

techniques, and how to track and

manage labware in storage

VWorks Knowledge Base

VWorks Automation Control

User Guide

Creating and editing VWorks

protocols and application interfaces

VWorks Knowledge Base

AssayMAP Syringe Test

Utility User Guide

Running a syringe test of the Bravo

96AM Head

Utility instructions in the

Protein Sample Prep

Workbench

Before you start

Safety information

4AssayMAP Bravo Platform Installation Guide

To access the context-sensitive help feature in the VWorks software:

1In the main window of the VWorks software, click the help button .

The pointer changes to . Notice that the different icons or areas are

highlighted as you move the pointer over them.

2Click an icon or area of interest. The relevant topic or document opens.

Service documentation

Agilent field service engineers can find instructions on how to maintain and

repair the Bravo Platform in the following guides:

•Bravo Platform Service Guide

•Pipette Head Service Guide

Changes to existing procedures and additional service procedures are published

as service bulletins and posted in the Support Portal at

http://intranet.chem.agilent.com/sites/partners/support/default.aspx

Safety information

Before installing and using the Bravo Platform, make sure you are aware of the

potential hazards and understand how to avoid being exposed to them. You

must be properly trained in the correct and safe installation and operation of

the device. For details, see the Bravo Platform Safety and Installation Guide.

Do not substitute parts or make any unauthorized modifications to the Bravo

Platform or the supplied safety equipment.

WARNING Changing or modifying the Bravo Platform safety equipment may prevent

the safe operation of the device, invalidate its safety compliance, and lead to personal

injury or property damage. Any customer who does not use the supplied safety equipment

or who modifies the supplied safety equipment assumes full responsibility for providing an

appropriate level of safety for its operators and for providing the applicable safety

compliance marking and documentation.

AssayMAP Bravo Platform

Installation Guide



Installing the platform

•“Overview of the hardware components” on page 2

•“Computer requirements” on page 3

•“Installation workflow for standard configuration” on page 4

•“Installing the Bravo risers” on page 5

•“Installing the 96AM Wash Station and Pump Module” on

page 10

•“Installing the standard platepads” on page 20

•“Installing the Peltier Thermal Station” on page 21

•“Installing and connecting the Orbital Shaking Station” on

page 26

Installing the platform

Overview of the hardware components

2AssayMAP Bravo Platform Installation Guide

Overview of the hardware components

The following figure and table show the components of the AssayMAP Bravo

Platform, which is required for running the AssayMAP protocols.

Figure AssayMAP Bravo Platform components

Item Required hardware

1Gripper upgrade

2Bravo 96AM Head

396AM Wash Station

4Pump Module 2.0 and two carboys

596AM Cartridge & Tip Seating Station

6Risers, 146 mm

7Peltier Thermal Station with STC controller

8Custom plate nest

9Red PCR Plate Insert

10 Orbital Shaking Station with Control Unit

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