Zeiss Smartzoom 5 User manual

Quick Guide

ZEISS Smartzoom 5

Referencing Application

ZEISS Smartzoom 5

Original Manual

Carl Zeiss Microscopy GmbH

Carl-Zeiss-Promenade 10

07745 Jena

Germany

[email protected]

www.zeiss.com/microscopy

Carl Zeiss Microscopy GmbH

Carl-Zeiss-Promenade 10

07745 Jena

Germany

Document Name: ZEISS Smartzoom 5 Quick Guide

Revision: 1

Language: en-US

Effective from: 11/2020

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 Content ZEISS

Content

1 Introduction .................................................................................. 4

1.1 Preparing Referencing ................................................................................ 4

2 User Interface................................................................................ 5

3 Referencing Tasks ......................................................................... 6

3.1 Stage (Angle-Stops).................................................................................... 6

3.2 Focus Position ............................................................................................ 8

3.3 Objective.................................................................................................... 10

3.4 Tilting Accelerometer ................................................................................. 11

3.5 Ov. Image Dist. Corr................................................................................... 12

3.6 Tilting Pivot Point ....................................................................................... 14

3.7 Image Alignment........................................................................................ 18

3.8 Stitching Referencing ................................................................................. 21

3.9 3D Referencing .......................................................................................... 22

4 Troubleshooting ............................................................................ 26

4.1 Angle stops not found................................................................................ 26

4.2 Overview distortion correction: Upright position......................................... 27

4.3 Stage does not move to the displayed position........................................... 28

4.4 Stitching Referencing: Referencing Object not found.................................. 28

4.5 Best Practice: Image Alignment .................................................................. 28

4.6 Wrong error message after PC upgrade...................................................... 32

Quick Guide ZEISS Smartzoom 5 | en-US | Rev.1 3

1 Introduction | 1.1 Preparing Referencing ZEISS

1 Introduction

The Smartzoom Referencing Application is used for improving the system accuracy and find-

ing the best settings for all components that are used. We recommend to perform the full refer-

encing workflow (meaning all referencing tasks are activated) if the system was newly installed.

If you update or change components we recommend to perform the full referencing workflow

again. As you have to change objectives and tilt the stand, read the Smartzoom 5 Instruction

manual, where these procedures are described in detail.

In case of mounting or dismounting the Transmittel Light Unit (TLU) repeat the referencing

tasks Ov. Image Dist. Corr. [

}

12] and Image Alignment [

}

18].

1.1 Preparing Referencing

To prepare the Smartzoom 5 system for referencing the following steps are required:

1. Switch on the microscope.

àMake sure that the stage is empty and has a clean surface.

2. Switch on the computer.

àAfter startup of the computer, wait for at least two minutes.

3. Start the Smartzoom Referencing application.

àWhile the software starts, the hardware is initialized. When the hardware was initialized

successfully you can start the referencing tasks.

Quick Guide ZEISS Smartzoom 5 | en-US | Rev.1 4

2 User Interface ZEISS

2 User Interface

3 4

Fig.1: Smartzoom Referencing Application User interface

1Status / Progress Area

Shows information on the system status, e.g. Software version or status of referencing.

2Referencing Tasks Area

Here you can activate/deactivate the referencing tasks which should be performed. By

clicking on the Start referencing button all activated referencing tasks will be per-

formed one after another.

3Preview Area

Shows the live image from the microscope camera. You will see the referencing proce-

dure of each step in here. Additionally, after starting a referencing task, you will see the

Referencing Wizard in a small dialog window in the upper right corner.

4Referencing Wizard

With the help of this wizard, you are able to control the referencing procedure of each

referencing step, e.g. Start/ Stop/ Continue buttons for controlling the referencing task.

The wizard contains instructions which will guide you through each step during a refer-

encing task. The instructions are shown in the blue box in the middle of the wizard win-

dow.

Quick Guide ZEISS Smartzoom 5 | en-US | Rev.1 5

3 Referencing Tasks | 3.1 Stage (Angle-Stops) ZEISS

3 Referencing Tasks

This chapter describes how to perform the referencing tasks available. We recommend to perform

the full referencing workflow (meaning all referencing tasks are activated) if the system was newly

installed. The entire workflow will take about 60 minutes with all objectives. When you exchange

a component (e.g. the optical engine) we recommend to perform the full referencing workflow

again.

3.1 Stage (Angle-Stops)

This referencing task will find the best settings for the microscope stage. Therefore the angle-

stops of the stage insert plate must be facing up. As you have to mount an objective, please read

the Smartzoom 5 instruction manual, where this procedure is described in detail. Note that you

should always start referencing with having mounted the objective with the highest magnification

(this depends on which objective you have bought).

1. Mount the objective with the highest magnification (e.g. 10.0x objective). Ensure that the

stage is empty and that the stand is in upright position. The angle-stops of the stage insert

plate must be facing up.

2. Click on the Start referencing button in the Referencing Tasks Area.

àThe Referencing Wizard will appear in the preview area.

Quick Guide ZEISS Smartzoom 5 | en-US | Rev.1 6

3 Referencing Tasks | 3.1 Stage (Angle-Stops) ZEISS

3. Click on the Start button within the wizard.

Quick Guide ZEISS Smartzoom 5 | en-US | Rev.1 7

3 Referencing Tasks | 3.2 Focus Position ZEISS

àNow the stage referencing will be performed automatically. You will see the stage mov-

ing to the referencing points one after another. The actual point is marked green.

After the Stage referencing (Angle-Stops) the wizard will continue with the next activated step.

3.2 Focus Position

This referencing task will find the best settings for the Z-axis / Focus Position.

1. Mount the 1.6x objective.

Quick Guide ZEISS Smartzoom 5 | en-US | Rev.1 8

3 Referencing Tasks | 3.2 Focus Position ZEISS

àEnsure that the stage is empty and that the stand is in upright position. The angle-stops

of the stage insert plate must be facing up.

2. Click on the Start button.

Quick Guide ZEISS Smartzoom 5 | en-US | Rev.1 9

3 Referencing Tasks | 3.3 Objective ZEISS

àReferencing of Z-axis is performed.

After the Z-axis referencing the wizard will continue with the next activated step.

3.3 Objective

This referencing task will find the best settings for the current used objective. We highly recom-

mend to perform referencing for each new objective that you use. Note that we will not describe

the referencing of the other objectives available as the procedure is exactly the same like de-

scribed for this objective.

Quick Guide ZEISS Smartzoom 5 | en-US | Rev.1 10

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