WPI MICRO-ePUMP User manual
WORLD
PRECISION
INSTRUMENTS
MICRO-ePUMP
Microinjector
Serial No._____________________
www.wpiinc.com
INSTRUCTION MANUAL
012220
MICRO-ePUMP
World Precision Instruments i
CONTENTS
ABOUT THIS MANUAL ................................................................................................................... 1
INTRODUCTION .............................................................................................................................. 1
Features....................................................................................................................................... 2
Benets........................................................................................................................................ 2
Applications................................................................................................................................ 2
Notes and Warnings................................................................................................................. 3
Parts List...................................................................................................................................... 3
Unpacking................................................................................................................................... 3
INSTRUMENT DESCRIPTION ........................................................................................................ 4
Front Panel ................................................................................................................................. 4
Rear Panel................................................................................................................................... 5
Foot Switch ................................................................................................................................. 6
Touch Panel Display ................................................................................................................. 6
Setting Menu.............................................................................................................................. 7
Setup............................................................................................................................................ 8
OPERATING INSTRUCTIONS.......................................................................................................12
Saving a Protocol.....................................................................................................................15
Loading a Protocol..................................................................................................................15
Changing the Pipette..............................................................................................................16
TECHNIQUES IN MICROINJECTION...........................................................................................16
Setting the Compensation Pressure ..................................................................................16
Manufacturing Micropipettes ..............................................................................................18
Calibrating Volume by Measuring Droplet........................................................................18
Calibrating Rate using a Known Volume ...........................................................................19
Multibarrel Microinjection ....................................................................................................20
MAINTENANCE ..............................................................................................................................21
Cleaning.....................................................................................................................................21
ACCESSORIES.................................................................................................................................21
Replacement Parts .................................................................................................................21
TROUBLESHOOTING ...................................................................................................................22
SPECIFICATIONS............................................................................................................................23
APPENDIX A: DROPLET VOLUME..............................................................................................24
Volume of a Micropipette .....................................................................................................24
BIBLIOGRAPHY..............................................................................................................................25
INDEX...............................................................................................................................................26
WARRANTY .....................................................................................................................................27
Claims and Returns ................................................................................................................27
Repairs.......................................................................................................................................27
Copyright © 2020 by World Precision Instruments. All rights reserved. No part of this publication may
be reproduced or translated into any language, in any form, without prior written permission of World
Precision Instruments, Inc.
ii World Precision Instruments
MICRO-ePUMP
World Precision Instruments 1
ABOUT THIS MANUAL
The following symbols are used in this guide:
This symbol indicates a CAUTION. Cautions warn against actions that can cause
damage to equipment. Please read these carefully.
This symbol indicates a WARNING. Warnings alert you to actions that can cause
personal injury or pose a physical threat. Please read these carefully.
NOTES and TIPS contain helpful information.
Fig. 1—MIRO-ePUMP Microinjector includes an internal pressurepump and the integrated
MICRO-ePORE Cell Penetrator technology.
INTRODUCTION
Designed to simplify intracellular injection and a variety of other microinjection tasks,
the MICRO-ePUMP uses carefully regulated air pres sure for injecting cells with uid.
Injected volumes range from picoliters to nanoliters. The port supplies positive pressure
for high-pressure ejection. The pressure port maintains a low positive “compensation”
pres sure to the injecting pipette between injection pulses to prevent uid up take
through capillary action.
Timing, injection pressure and compensation pressure are adjusted independent-
ly using the touch screen interface. Time intervals can range from 2 seconds down
to 10ms or less, depending on the injection pressure setting. The injection pressure
interval is triggered using the foot switch.
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The MICRO-ePUMP is designed to inject very small quantities of uids, such as drugs,
into cells or small organelles. Pressure injection is an especially useful alternative to
electroionophoresis, since it does not mandate the use of charged ions. Two dierent
positive pressures may be applied–one for ejection at high pressure and a second,
lower pressure to prevent back lling of the pipette by capillary action.
WPI’s MICRO-ePORE™ Pinpoint Cell Penetrator technology is embedded inside the
MICRO-ePUMP. When the researcher enables the The MICRO-ePORE™, it delivers
a highly localized voltage signal to a targeted injection site to facilitate penetration
with minimal trauma. The researcher determines the amplitude and frequency of the
signal that best suits the application. The signal originates in the control box, and it
is transmitted through the electrode interface cable to the microelectrode holder.
A silver wire is used to transmit the signal into the electrically conductive substance
being injected. A reference electrode is used to place the media at 0.0 V potential with
reference to the generated voltage.
The MICRO-ePUMP oers separate regulated compensation (back lling prevention)
and ejection pressures with a precision timing circuit that switches from injection
pressure to compensation pressure automatically.
Features
• Regulated compensation and injection pressure
• Pressure output: 0.3-87 PSI
• Onboard MICRO-ePORE™
• Pinpoint Cell Penetrator for targeted microinjection and increased viability of
injected embryos
• Audio continuity tone indicating active probe
• Injection counter to indicate total number of injections
• User-adjustable frequency and voltage through touch screen
• Foot switch to activate voltage for MICRO-ePORE™ and injection
• Low volume tubing assembly
Benets
• Internal pressure source
• Intuitive user-interface for injector and MICRO-ePORE™
• Small footprint takes up very little bench space
• Easy to navigate with touch screen and control knob
• Inject into a single cell in picoliter volumes
Applications
• Microinjection of diverse compounds and biomolecules – DNA, RNA, proteins
• Pre- and post-implantation in embryos of various species – mice, rats, monkeys,
bovine, pigs, zebrash, etc.
MICRO-ePUMP
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Notes and Warnings
!WARNING: SECURE THE PIPETTE FIRMLY IN THE HOLDER. WHEN HIGH
PRESSURE IS APPLIED, A LOOSE PIPETTE CAN BE EJECTED FORCEFULLY.
DO NOT APPLY PRESSURES IN EXCESS OF 100 PSI (100 KPA).
!WARNING: THIS INSTRUMENT IS FOR INVESTIGATIONAL USE ONLY IN
ANIMALS OR OTHER TESTS THAT DO NOT INVOLVE HUMAN SUBJECTS.
Parts List
After unpacking, verify that there is no visible damage to the instrument. Verify that all
items are included:
(1) MICRO-ePUMP
(1) Foot switch
(2) 300863 WPI MICRO-ePORE™ Electrode Holders (Electrode holder includes an
electrode holder startup kit.)
(1) 300744 MICRO-ePUMP Capillary Kit which includes:
(4) 1.0 mm pipette gaskets (green)
(4) 1.2 mm pipette gaskets (black)
(4) 1.5 mm pipette gaskets (red)
(4) 1.65 mm pipette gaskets (white)
(1) Pipette handle
(1) MICRO-ePORE injection assembly
(2) Sealing o-rings
(1) Pipette holder
(1) MICRO-ePUMP tubing assembly
(1) MICRO-ePUMP adapter tubing assembly
(1) AC/DC 24 V Power Adapter
(1) Instruction Manual (available online at www.wpiinc.com/manuals)
Unpacking
Upon receipt of this instrument, make a thorough inspection of the contents and
check for possible damage. Missing cartons or obvious damage to cartons should be
noted on the delivery receipt before signing. Concealed damage should be reported
at once to the carrier and an inspection requested. Please read the section entitled
“Claims and Returns” on page 27 of this manual. Please contact WPI Customer
Service if any parts are missing at (941) 371-1003 or [email protected].
Returns: Do not return any goods to WPI without obtaining prior approval (RMA
# required) and instructions from WPI’s Returns Department. Goods returned
(unauthorized) by collect freight may be refused. If a return shipment is necessary, use
the original container, if possible. If the original container is not available, use a suitable
substitute that is rigid and of adequate size. Wrap the instrument in paper or plastic
surrounded with at least 100 mm (four inches) of shock absorbing material. For further
details, please read the section entitled “Claims and Returns” on page 27 of this manual.
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INSTRUMENT DESCRIPTION
Front Panel
Touch screen interface
Adjustment Knob
Injector Port
MICRO-ePORE™ Interface Cable Port
Fig. 2—The major features of the MICRO-ePUMP are labeled in this diagram.
Touch Screen Interface – The responsive panel allows for intuitive control. Touch
the injection counter to reset it, the tank pressure gauge to rell the tank, Clear Tip to
blow a blast of air through the pipette tip, a parameter to modify it using the knob, or
the settings area to adjust the settings.
Adjustment Knob – Rotate the adjustment knob to change the highlighted
parameter. Press it to toggle between gross and ne tune controls.
MICRO-ePORE™ Interface Cable Port – If you are using the MICRO-ePORE™,
connect the Microelectrode Interface Cable (probe cable) to this port. Press the
connector into the port. This cable is labeled as Front Panel.
Fig. 3—The Interface Cable Connector is labeled “Front Panel.” Do NOT plug it into the foot
switch port on the rear panel.
Injector Port – Plug the easy connect connector on the tubing into this port.
MICRO-ePUMP
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Rear Panel
Foot Switch Connection
USB Port (not used)
Power Supply Connection
Power Switch
Fig. 4—The foot switch connection, power supply connection and
power switch are located on the back panel of the MICRO-ePUMP.
Foot Switch Connection – Plug the connector from the foot
switch into the connection port marked with the foot switch icon.
Press the foot switch to initiate a timed sequence (timed mode)
or to manually eject pressure (manual mode). This foot switch is a
two-stage switch. Depress it half way down to engage the MICRO-
ePORE™, and depress it fully to make an injection. (See “Foot
Switch” on page 6.)
USB Port –This interface is reserved for factory use only.
Power Switch –To power on the unit, press the toggle switch to
—. To power o the unit, press the toggle switch to O.
Power Supply Connection –Insert the power supply connector
into this port and plug the other end into an A/C wall outlet.
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Foot Switch
The foot switch is a two-step switch. Press the switch half way down to activate the
MICRO-ePORE™. If the MICRO-ePORE™ is disabled, just press the switch all the way
down. When the switch is depressed all the way, the MICRO-ePUMP is activated.
Not depressed Half way depressed Fully depressed
Touch Panel Display
MICRO-ePORE™ Controller
Frequency
Amplitude
Injection Controller
Injection Pressure
(PSI or kPa)
Compensation Pressure
(PSI or kPa)
Injection Time
(msec - 2 s max.)
Protocol Loaded
Injection Counter
Tank Pressure Gauge
Clear Clogged Tip
Settings Screen
2
Fig. 5—The Touch Panel Display gives you access to all the parameters and the settings menu.
Protocol Loaded – The number in the box in the upper left corner indicates the
protocol with stored parameters that is loaded. You can store and recall up to three
of your most used protocols in the MICRO-ePUMP memory. If the box is empty, no
protocol is loaded.
Injection Counter – Every time you depress the foot switch all the way (when the port
is enabled), the injection counter increments. To reset the injection counter to zero,
touch the injection counter on the display.
Tank Pressure Gauge – The internal pressure tank is pressurized to 100 PSI. When
the pressure in the tank falls below 90 PSI (90%), the tank automatically rells. This
10% is considered the tank reserve. The Tank Pressure Gauge shows the status
of the tank reserve as the pressure falls to 90%. If you want to manually start the
repressurization of the tank, touch the Tank Pressure Gauge. When you power up
the MICRO-ePUMP, the gauge may register below 90% as the tank lls initially. The
display will readout in PSI until the tank reaches 90 PSI. Then, the display will show the
percentage of the reserve pressure (0 – 100%).
Clearing Clogged Tip – Press Clear Tip to force a 500 ms burst of maximum pressure
(87 PSI_ through the injection tip. This can be used if the tip becomes clogged.
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