VWR XT96 User manual

Instruction manual

VWR®PCR Thermal Cycler XT96 / XTender96

EU cat. no US cat. no

XT96 732-1359 76452-151

XT96 Gradient 732-3428 76452-153

XTender96 732-3657 76452-155

XTender96 Gradient 732-3658 76452-157

___

Version 2 Issue 17 October 2021

2vwr.com I Instruction manual VWR®PCR Cycler XT96

Legal address of Manufacturer

Europe

VWR International bv,

Researchpark Haasrode 2020

Geldenaaksebaa

n 464 B-3001

Leuven

+3216385011

be.vwr.com

UK Importer:

VWR International Ltd

Hunter Boulevard, Magna Park

Lutterworth, Leicestershire, LE17 4XN

uk.vwr.com

United States

VWR International LLC

100 Matsonford Rd

Radnor, PA 19087

+1 800-932-5000

www.vwr.com

Country of Origin : GERMANY

3vwr.com I Instruction manual VWR®PCR Cycler XT96

CONTENTS

INTRODUCTION 4

GENERAL WARNING 4

SAFETY INSTRUCTIONS 4

LICENCE NOTICE 5

DELIVERY CONTENTS 6

UNPACKING 6

SPECIFICATIONS 7

CONNECTIONS 8

INSTALLATION OF THE PCR CYCLER XTENDER96 9

OPERATING THE PCR CYCLER XT96 10

Main Menu 12

Change User 13

Incubation Mode 14

Run dialogue 15

Run dialogue: Overview – “Simple Mode” 15

Run dialogue: Overview – “Expert Mode” 16

Program Dialogue 22

Deleting a command from the program 25

Available commands (program steps) 26

GLPs dialogue 28

Explorer dialogue 30

Diagnose dialogue32

System dialogue 33

MAINTENANCE 41

Cleaning, maintenance and repair of the thermal cycler 41

Returns only in original packaging 41

Temperature check-up 41

Calibration 41

ORDERING INFORMATION 42

TECHNICAL SERVICE 42

WARRANTY 42

COMPLIANCE WITH LOCAL LAWS AND REGULATIONS 42

DISPOSAL INSTRUCTION 43

LOCAL VWR OFFICES IN EUROPE AND ASIA PACIFIC 44

4vwr.com I Instruction manual VWR®PCR Cycler XT96

INTRODUCTION

The VWR thermal cyclers are the ideal PCR devices for all molecular biological and biochemical

laboratories working in the field of basic research or routine diagnostics. The 250°C HTR (High

Temperature Range) Peltier technology allows quick changes in temperature up to 4 °C/sec (max). The

individual monitoring and controlling of each of the eight Peltier elements per block equipped with

'Long Life Technology' ensure the tightest correlation of target and actual temperature across the

entire thermal plate leading to highest reproducibility of the results. To avoid condensation each block

is equipped with a heated lid.

The VWR thermal cyclers are equipped with a touch-sensitive, graphic coloured TFT display of the

newest generation, allowing operation of the device by simply touching symbols and graphic elements

on the screen. This leads to easy and intuitive handling and programming of the device. Additionally,

a mouse can be connected via USB.

Furthermore, an Ethernet port is available to connect the devices with Microsoft Windows®or Linux

networks. It is therefore possible to use an external server for archiving programs and GLP reports

which will be available globally in the network (e.g. for other thermal cyclers).

Transfer of data can be done by using commercially available USB memory sticks enabling a nearly

unlimited saving space for programs and GLP reports, which can be printed using a printer connected

to the thermal cycler via the network.

Notice: The USB ports support only standard cable mice and standard USB sticks.

The maximum acceptable length of the connecting cable of the port is 3 m!

GENERAL WARNING

The PCR Cycler XT96 has been designed for laboratory use and it should not be used at temperature

below 15 °C. If the instrument is at a temperature below 10 °C place it in a warmer room and wait at

least two hours before using it. Before plugging the instrument into a power supply, check that the

voltage and frequency on the label matches the power supply specifications. Before any operation

ensure that the ventilation grids are not obstructed.

SAFETY INSTRUCTIONS

The PCR Cycler XT96 is designed with function, reliability, and safety in mind.

Before using the PCR Cycler XT96 please read this entire manual and strictly follow the safety

instructions!

Intended use of the PCR Cycler XT96: Working environment for DNA amplification during a Polymerase

Chain reaction (PCR).

Safety and protection of the user cannot be ensured if the PCR Cycler XT96 is used in any manner that

is not specified in these instructions. Special notice must be taken of the following:

Returns only in original packaging

Please use the manufacturer's original package to ensure a safe return.

5vwr.com I Instruction manual VWR®PCR Cycler XT96

Caution: Dangerous voltage

Ensure that the voltage requirements of the PCR Cycler XT96 exactly match your local

AC power supply.

Only use the original AC cable supplied with the PCR Cycler XT96.

Caution: Liquids

Ensure that no liquids can enter the device. Reaction tubes must be filled outside of the

thermal cycler.

Caution: Dangerous explosive material

Do not use reactive or explosive substances in the PCR Cycler.

Caution: Damage

Check for transport damage and completeness of the accessories upon arrival. If any

damage is noticed, contact your distributor or manufacturer immediately. If you detect

any damage of the PCR Cycler XT96 or one of its accessories, do not connect the PCR

Cycler to the AC wall outlet and do not use the device in any manner!

Caution: Hot surfaces

The thermal plate, the heated lid and the reaction tubes quickly reach temperatures

above 50 ºC / 122 ºF. There is danger of burning! Keep the lid closed until the temperature

reaches 30 °C / 86 ºF or less. Only use materials (tubes, tube caps, plates and sealing

films) which are heat resistant to 120 ºC / 248 ºF.

Caution: Environment

The ventilation of the device must not be covered.

Danger: Line voltage

Incorrectly grounded products can be extremely hazardous in the event of a fault.

Use only a 3-conductor power cable (3x 0.75 mm2) with protective ground. The power

connector may only be plugged into a socket with a protective ground. The protection

must not be nullified by an extension cable without protective ground.

LICENCE NOTICE

This instrument is licensed for research and development and for uses other than human in vitro

diagnostics under one or more of the following patens of Applera Corporation: U.S. Patent Nos.

5,656,493, 5,038,852, 5,333,675, 5,475,610 (claims 1-159 and 164-166), 6,703,236 (claims 7-10) and

7,238,517 or corresponding claims in their non-U.S. counterparts. No right is conveyed expressly, by

implication or by estoppels under any other patent of Applera, including but not limited to U.S. Patent

No. 6,814,934 and its non-U.S. counterparts, which describe and claim thermal cyclers capable or real-

time detection.

6vwr.com I Instruction manual VWR®PCR Cycler XT96

DELIVERY CONTENTS

After receipt of the system, please check if the following items are included in the shipment. If any

transport damage is noticed, contact your distributor or manufacturer immediately.

IMPORTANT NOTICE: IF YOU FIND ANY DAMAGE OF THE DEVICE, DO NOT USE THE DEVICE!

1 Thermal cycler

1 Power cord

1 Instruction manual

1 Link PC-software VWR 'PCR Cycler XT Software'

UNPACKING

Remove the instrument from its packaging and inspect it for signs of damage. If any are

discovered, inform your supplier immediately

The instrument must be placed on a stable, level surface and positioned such that air can

circulate freely around the casing

Ensure your proposed installation site conforms to the environmental conditions for safe

operation

There should be enough space to make sure that the ventilation grills on the front and back

are not covered and sufficient ventilation is provided. The accessibility of the power switch

must not be hindered. There should be a distance of at least 25 cm (10 inch) to the wall or the

next device. Two thermal cyclers should not be placed back-to-back or back to front.

The instrument is designed for indoor use only, temperature range of 10 to 35 °C and

humidity of 35 to 70% are recommended

If the instrument has just been unpacked or has been stored in a cold environment, it should

be allowed to come to room temperature for 2 – 3 hours in the laboratory before switching

on. This will prevent calibration failure because of internal condensation

The instrument must be connected to the power supply with the power cord supplied.

It is recommended that users should thoroughly read this manual prior to use

Contact your supplier if you experience any difficulties with this instrument

7vwr.com I Instruction manual VWR®PCR Cycler XT96

SPECIFICATIONS

VWR PCR Cycler XT96

•8 Peltier elements with Long-Life-technology and 8 control circuits with Pt 1000 temperature

sensors

•Maximal heating and cooling rate: 4 °C/s

•Block uniformity (at 72 °C): ± 0.35 °C

•Temperature range thermoblock: 4 to 105 °C

•Control accuracy thermoblock: ± 0.2 °C

•Variable ramping: 0.1 to 3.0 °C/s

•Increment/Decrement time: 0:00:01 to 0:59:59

•Increment/Decrement temperature: 0.1 to 3 °C

•Lid heating temperature range: 40 to 110 °C

•2 x USB, 1 x Ethernet interfaces (MS Windows®or Linux)

Note: USB interfaces only support standard USB sticks, cord mouse and keyboards!

•Dimensions (B x H x D) : 22 x 30 x 33 cm (XTender: 22 x 20 x 33 cm)

•Weight incl. block: 8.9 kg (XTender:8.3 kg)

•Pollution rate: 2

•Environmental temperature range: +10 °C to +35 °C

•Max. relative humidity: 70 %

•Maximum height above sea level: 2000 m

•Noise level: < 37 dB (A)

Gradient feature (optional)

•Maximal gradient range over 8 columns, respectively: 30 °C (± 15 °C)

•Temperature range Gradient: 30 to 100 °C

•Gradient accuracy: ± 0.2 °C

User interface/functions

•Touch sensitive (capacitive) TFT-Display (800 x 480) (not included in the XTender)

•Direct help function

•Internal flash memory for 500.000 typical PCR programs in free configurable

folders/subfolders. Unlimited number of programs via network PC or USB stick

•Free PC software for the generation of PCR programs on the computer

•Unlimited number of steps per program

•Password protected user accounts with variable access rights

•GLP reports for complete recording of all runs

•QuickStart function of the last used program

•Automatic restart after power failure, Power-Fail-Denaturation, Instant Incubation

•Remote control and monitoring of instruments via PC-software

•MP3 sounds free of choice

•Master-/Slave-control

Block capacity

•96 well Universal block with lid heating and automatic height adaption for 96 x 0.2 ml tubes

and 96 well PCR plates (non-skirted and half-skirted)

Power supply

100 - 240 V AC, 50/60 Hz, max. 860 VA

For indoor use, only!

8vwr.com I Instruction manual VWR®PCR Cycler XT96

CONNECTIONS

1x USB interface

Ethernet interface

AC Power inlet

Status LED

Main power switch

9vwr.com I Instruction manual VWR®PCR Cycler XT96

Before first use, ensure that the voltage requirements of the PCR Cycler exactly match your local AC

power supply. Connect the PCR Cycler to the AC power supply only by the AC power cord delivered

with your PCR Cycler XT. Only connect the PCR Cycler XT to electric power systems with an earth

conductor. The AC power inlet is located at the rear of the Cycler. To disconnect the cycler

from the AC power supply, remove the AC power cord from the AC power inlet. The

accessibility of the AC power inlet must be not hindered or obscured in any way.

If a service is required, contact your local distributor or the manufacturer (see "TECHNICAL SERVICE

AND ORDER INFORMATION", page 36).

The serial number of the PCR Cycler is located underneath the instrument. Unauthorised work on the

device voids the warranty.

The surface of the device should be decontaminated with a lint-free cloth soaked with 70% ethanol

before sending. Please fill in and sign the decontamination certificate and send it to the

manufacturer together with the device.

For safety reasons the device must be switched off and the mains plug pulled before cleaning is

performed!

INSTALLATION OF THE PCR CYCLER XTENDER96

The VWR thermal cycler XTender96 is a fully equipped PCR Thermal cycler without a Touch screen, the

ideal device controlled by a PC or from another XT96 Thermal cycler.

To connect multiple XTender96 Thermal Cyclers, you need:

- An Ethernet cable for each XTender96 Thermal Cycler

- A network or an external network router (DHCP server is required)

Please ask your network administrator for the required settings.

Set up the Connections

1. Connect the Ethernet cable to the back of the thermal cycler and connect the other end of the

cable to the network.

2. Repeat step 1 for each thermal cycler.

3. Power on the XTender96 thermal cycler.

4. Plug the “Network Configuration USB-Stick” into an USB port of the XTender96 thermal cycler.

Note: For each XTender96 thermal cycler, a new “XT96ConfigFile” file must be created.

5. The XTender96 Thermal Cycler will recognize the USB stick and set up the network configuration

automatically. After finalization, the instrument will switch of.

6. Remove the “Network Configuration USB-Stick” from the USB port of the XTender96.

7. Power on the XTender96 thermal cycler

8. Follow the instructions in “Remote Control” on page 33 and 34 of the manual to enable a

remote control from a XT96 Thermal cycler or from a PC.

10 vwr.com I Instruction manual VWR®PCR Cycler XT96

Generate a “Network Configuration USB-Stick”

1. Power on a XT96 Thermal Cycler (Master unit)

or start the XT96 PC Software on your PC.

2. Use the “System” dialogue in the Software and

click on “Thermal Block” and “Configure USB

Stick”

3. Insert an USB Stick into the Cycler or PC.

4. Configure the network settings for the

XTender96 thermal cycler, as shown in the

picture.

5. Make sure each instrument name is unique and press OK.

6. A “XT96ConfigFile” file will be generated and stored on the USB stick.

7. Use this “Network Configuration USB Stick” to set up the Network configurations on your

XTender96 Thermal Cycler.

Note: Every XTender96 Thermal Cycler needs a separate “XT96ConfigFile” file where the

instrument name is unique.

OPERATING THE PCR CYCLER XT96

General operation

The operation of the thermal cycler can be run by the internal touch screen, by using the touch sensitive

surface of the display or by using a mouse connected to an available USB port. Furthermore, the

thermal cycler can also be controlled by computer integrated to a network. The supplied application

software offers the same functions as the internal thermal cycler software, except for some system

settings.

If the user has to enter numbers or text the corresponding field has to be touched on the touch screen

or clicked with the mouse. An appropriate keyboard will appear on the screen according to the

expected entry.

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