Precision 51221076 Supplement

Installation/Service Manual

Dubnoff & Shallow Form

Shaking Water Baths

Precision

170 Marcel Drive

Winchester, VA

USA

Phone: 540-869-8623

Toll Free: 800-621-8820

FAX: 540-869-8626

ManualP/N36100126(34002554)

Rev.BDated08NOV01

High Temp.

Off

NOTICE

THEMATERIALINTHISMANUALISFORINFORMATION PURPOSESONLY. THECONTENTS

AND THE PRODUCT IT DESCRIBES ARE SUBJECT TO CHANGE WITHOUT NOTICE.

PRECISION MAKES NO REPRESENTATIONS OR WARRANTIES WITH RESPECT TO THIS

MANUAL. IN NO EVENT SHALL PRECISION BE LIABLE FOR ANY DAMAGES, DIRECT OR

INCIDENTAL, ARISING OUT OF OR RELATED TO THE USE OF THIS MANUAL.

For repair information or replacement parts assistance from the manufacturer, call Customer Service using our toll

freetelephonenumber.

800-621-8820

(FAX) 540-869-0130

INDEX DATE NOTES

REVISION STATUS

Initial release

AMENDED PAGES

6/99

B NOV01 Add caution "acidic & caustic

substance....."

Contents

INTRODUCTION.......................................................................................................................1

UNPACKING AND DAMAGE ...................................................................................................1

GENERAL INFORMATION.......................................................................................................2

PERFORMANCE ......................................................................................................................2

INSTALLATION ........................................................................................................................3

WATER LEVEL REGULATOR .................................................................................................4

EXPLANATION OF CONTROLS..............................................................................................6

OPERATION .............................................................................................................................7

MAINTENANCE........................................................................................................................8

TROUBLESHOOTING ..............................................................................................................9

PARTS REPLACEMENT ........................................................................................................14

TEMPERATURE CALIBRATION ...........................................................................................17

REPLACEMENT PARTS LIST ...............................................................................................18

ASSEMBLY & SCHEMATIC DWGS ......................................................................................20

WARRANTY ...........................................................................................................................29

INTRODUCTION

Your satisfaction and safety are important to

PRECISION and a complete understanding of

this unit is necessary to attain these objectives.

As the user of this apparatus, you have the

responsibility to understand the proper function

andoperationalcharacteristicsofyourbath. This

instructionmanualshouldbethoroughlyreadand

all operators given adequate training before

attemptingtoplacethisunit inservice. Awareness

of the stated cautions and warnings, and

compliance with recommended operating

parameters — together with maintenance

requirements — are important for safe and

satisfactoryoperation. Theunitshouldbeusedfor

itsintendedapplication;alterationsormodifications

will VOID THE WARRANTY.

WARNING

AS A ROUTINE LABORATORY PRECAUTION,

ALWAYS WEAR SAFETY GLASSES WHEN

WORKING WITH THIS APPARATUS.

Thisproductisnotintended,norcanitbeused,

as a sterile or patient connected device. In

addition,thisapparatusisnotdesignedforuse

in Class I, II or III locations as defined by the

NationalElectricalCode.

UNPACKING AND DAMAGE

Save all packing material until unit is put into

service. Thismerchandisewascarefullypacked

andthoroughly inspectedbefore leavingourfac-

tory.

Responsibility for safe delivery was assumed by

the carrier upon acceptance of the shipment;

therefore,claimsforlossor damagesustained in

transit must be made upon the carrier by the

recipient as follows:

Visible Loss or Damage: Note any external

evidence of loss or damage on the freight bill, or

expressreceipt,andhaveitsignedbythecarrier's

agent. Failuretoadequatelydescribesuchexter-

nal evidence of loss or damage may result in the

carrier's refusing to honor your damage claim.

Theformrequiredtofilesuchclaimwillbesupplied

by the carrier.

ConcealedLossorDamage: Concealedlossor

damage means loss or damage which does not

becomeapparentuntilthemerchandisehasbeen

unpacked and inspected. Should either occur,

make a written request for inspection by carrier's

agentwithinfifteen(15)daysofthedeliverydate;

thenfileaclaimwiththecarriersincethedamage

is the carrier's responsibility.

If you follow the above instructions carefully, we

will guarantee our full support of your claim to be

compensated for loss or concealed damage.

DO NOT — FOR ANY REASON — RE-

TURN THIS UNIT WITHOUT FIRST

OBTAINING AUTHORIZATION. In any

correspondence to

PRECISION,

please

supply the nameplate data, including cata-

log number and serial number.

GENERAL INFORMATION

ThePrecisionDubnoffMetabolicShakingIncubator

Bath and the Precision Shallow-Form Shaking

Bathhavebeendesignedprimarilyfortheincubation

of tissue and homogenates.

The microprocessor control panel houses all

functions necessary to operate the bath. The five

push-button switches and single display window

allow the operator to adjust bath temperature,

motor speed, and calibration via a single set of

controls.

The proportional integral derivative temperature

control allows precise temperature control from

5°C above ambient to 55°C without cover and to

99.9°C with use of the gable cover provided.

A high limit thermostat is provided and can be set

to prevent heater runaway in the event of

temperature control failure.

Trayshakingspeed is easily adjusted between 30

and 200 cycles per minute at 5 cpm intervals.

The tray stroke can be adjusted to three different

lengths: .5", 1" and 1.5". The drive mechanism

containsself-lubricatingbearingswhichrequireno

maintenance.

The tray is easily removable and rides silently on

four plastic balls.

The interior of the bath is constructed of stainless

steel and is designed for operation with distilled

water or water solutions, such as water/ethylene

glycol. Donot use deionizedwater. Ifdeionized

is the only water available, a mixture with a 50/50

ratio of deionized and tap water should be used.

Deionized water (18 MEG) will damage metals.

The body is made from galvanized steel and is

painted for added protection. A drain is located at

the far left hand end of the bath. A stainless steel

gable cover is also provided with the bath.

The230voltunitsareidenticalinappearancetothe

115voltunitsexceptfortheadditionofastep-down

transformer for the motor.

PERFORMANCE DATA

Thefollowing table identifies thespecificationsfor

the Shallow Form and Dubnoff Shaking Baths.

Performance Data

With Cover Without Cover

Uniformity 37°C ±.05 ±.15

56°C ±.05 ±.2

Sensitivity 37°C ±.05 ±.1

56°C ±.05 ±.1

Listing of Models included in this Manual

Cat.No. Description Volts Hertz Watts Amps

51221076 Dubnoff Metabolic

Shakin

Incubator

Bath

120 50/60 1000 8.3

51221077 230 50/60 1000 4.3

51221079 Shallow Form

Shakin

Bath

120 50/60 1000 8.3

51221078 230 50/60 1000 4.3

INSTALLATION

WARNING

INSTALLATION SHOULD BE COMPLETED BY

QUALIFIEDPERSONNELONLY.

Location - The most uniform operating conditions

willbeobtainedbyplacingthebathonalevelsurface

in an area remote from drafts, ventilating outlets,

radiators, and other rapidly changing ambient

conditions.

Electrical Connections -

IMPORTANT

FOR PERSONAL SAFETY, THIS APPARATUS

MUSTBEPROPERLY GROUNDED.

1. Thepowercordprovidedonthisunitisequipped

withathree-prong(grounding)plugwhich mates

with standard three-prong grounding wall

receptacletominimizethepossibilityofelectric

shockhazardfromthisapparatus. Ifindoubtthe

usershouldhavethewallreceptacleandcircuit

checkedbyaqualifiedelectriciantomakesure

the receptacle can provide adequate current

andisproperly grounded.

2. Whereastandardtwo-prongwallreceptacleis

encountered, it is the personal responsibility

andobligationoftheusertohaveitreplacedwith

aproperlygroundedthree-prongwall

receptacle.

Do not, under any circumstances, cut or remove

the third (ground) prong from the power cord. Do

not use a two-prong adapter plug.

Determinethetotalamountofcurrentbeingusedby

otherapparatusconnectedto the circuit that will be

used for this apparatus. It is critical that the added

current demand (see nameplate) of this and other

equipmentusedonthesamecircuitdoesnotexceed

theratingof the fuse or circuit breaker.

CAUTION

BESURETHATTHEPOWERSUPPLYISOFTHE

SAME VOLTAGE AS SPECIFIED ON THE

NAMEPLATE.

Shaker Clip Installation - The spring retainers

providedaredesignedtofirmlyholdflaskclipsinthe

shakingtrayholes.

1. It is important that these retainers be inserted

into tray holes before inserting optional flask

clips.

Stroke Length Adjustment - The length of the

shakerstrokeissetatthefactoryfora1"stroke. The

following procedure should be observed when

changingstrokelengths.

WARNING

TURN OFF POWER TO THE BATH AND

DISCONNECT THE BATH FROM ITS POWER

SUPPLY.

1. Togainaccesstothemotordrivehub,remove

four screws in upper side panel corners, and

carefullyopenthecontrolpanel.Onceopened,

the 3-position drive hub will be immediately

visibleattop-centerofcontrolhousing.

2. Toalterthestrokelength,unscrewthebearing

mounting screw from its present position and

rethread the screw firmly into the hole that will

provide the desired stroke length. The hole

closestto the hub centerprovidesthe shortest

stroke;theonefurthestaway,thelongest. The

threethreadedopeningsprovidestrokelengths

of 0.5", 1.0", and 1.5".

3. After selecting desired stroke length, close

control panel and replace the four screws.

Reconnectthebathtopowersource.

Dubnoff Flowmeter Assembly (Optional) -

1. Theoptionalflowmeterassemblyisinstalledby

placingthedovetailonthefrontoftheflowmeter

intothematingbracketlocatedonthebackright

sideofthebath.Theflowmeteriscalibratedfor

use with oxygen, nitrogen, and CO2 and is

scaledtoreaddeliveryrates upto10standard

cubicfeet perhour.

2. Connect the bottom hose barbs to the gas

sources. The top hose barbs connect to the

gassing hood(s) with the tubing provided. A

valveisconnectedtoeachoftheflowmetersfor

moreprecisecontroloftheatmosphere.

WATER LEVEL REGULATOR

(OPTIONAL)

INSTALLATION INSTRUCTIONS - Use the

followingstepsalongwithFig.1&2shownonthe

followingpage.

1. Turn off unit, disconnect power & drain water

fromthe bath.

2. Unscrew drain plug from side of bath body.

3. Replacewithelbowconnector(shippedloose).

NOTE

TO INSURE GOOD SEAL, WRAP THREADS

WITHTEFLONTAPEOREQUIVALENTSEALER.

4. Tighten elbow connector so open end (tube

fitting)facesstraightdown.

5. PlaceWaterLevelRegulatorBkt.Ass'yonthe

lip of the bath pan (drain side of the bath

towardstherearoftheunit).Thesmallflange

with gasketing near the top of the bracket

shouldrestagainsttheinsideofthebathpan.

Theother2flangeswithgasketingshould rest

againsttheoutsideofthebathbody.

6. Insert the plastic tubing (coming from the

bottom of the water level regulator) into the

elbow connector (at the drain) & tighten the

elbowconnectorfittingfirmlytoinsureagood

seal.

7. Uncoil Tygon tubing & press onto 1/4" Hose

Barb Fitting (located at the top of water level

regulator).

8. Hook-upotherendofthetygontubingtowater

supply&turnonwater.(Waterpressureshould

fall within the following range: MIN is approx.

15 PSI, MAX is approx 75 PSI)

NOTE

WATERLINEMARKINGONTHEWATERLEVEL

REGULATOR IS AN INDICATOR OF THE

APPROX. WATER LEVEL THAT WILL BE

MAINTAINED IN BATH. THE ASS'Y HAS BEEN

SETTOMAINTAINAPPROX.1-3/4"OFWATER.

TO RAISE THE SET WATER LEVEL, LOOSEN

THUMBSCREW & RAISE WATER LEVEL

REGULATOR TO DESIRED LEVEL (COINCIDE

WITH WATER LINE MARK) & TIGHTEN

THUMBSCREW TO HOLD WATER LEVEL

REGULATORINPLACE.

NOTE

DO NOT OVER-TIGHTEN THUMBSCREW

WATER LEVEL REGULATOR ASSEMBLY

FIGURE 2

CUTAWAY FRONT VIEW OF BATH

FIGURE 1

SIDE VIEW OF BATH

Set Point

Actual

BathTemp., °C

Speed,RPM

BathTemp., °C

START/STOP

SELECT/ENTER

Temp.

Offset

HeaterOn

Shaker

PressSELECT/ENTERagain to setthenew value.

PressSELECT/ENTER button toset point.

PressSTART/STOP tobegin or end shaking.

Press or untilvalue is displayed.

Toadjust temperature orshaking speed :

FIGURE A

EXPLANATION OF CONTROLS

Power Switch - The power switch is located on

the lower right hand side of the bath, it provides

power for the entire unit.

Hi-LimitThermostat-TheHi-LimitThermostatat

is located immediately to the left of the power

switchonthelowerrighthandcornerofthebath.

The high limit thermostat provides prevention of

heaterrunawayintheeventoftemperaturecontrol

failure.

The Control Panel

islocatedontop

ofthe bath.

1. LEDDisplay-The

three-digit LED

display is used

toshowboththe

actual and

setpoint values

for temperature

and shaking

speed.

2. HeaterOnLamp-

The "Heater

ON" lamp is

illuminated

when power is

applied to the

heater.

3. Indicator Lamps - These lamps indicate

which of 4 parameters (Actual Temp, Actual

Speed,SetpointTemp,andSetpointSpeed)

is displayed in the LED window.

4. Enter Key - The Enter Key is used to choose

which parameter is displayed in the LED window,

and is also used to store a new setpoint value.

5. UP & DOWN Keys - are used to adjust

temperature

andspeed

setpointvalues.

Pressing &

holdingthese

keys will

increment/

decrementthe

displayvalue

slowly at first,

and then rapidly

ifheld.

6. Offset Key-

is used to

adjustthe

temperature

to match a

calibrated

reference

thermometer.

7. Shaker

Start/ Stop Key

-This key is

usedto toggle

the tray shaking

motion on or

off.

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