Optika Italy B-380 Series User manual
Version: 3
Issued: 18, 03, 2015
Model
B-383POL
B-380 Series
INSTRUCTION MANUAL
Page 2
Table of Contents
Warning
Symbols and conventions
Safety Information
Intended use
Package Contents
Unpacking
Product Specifications
Overview
Using the microscope
Maintenance
Troubleshooting
User replaceable accessories and spare parts
Equipment disposal
Page 3
Warning
This microscope is a scientic precision instrument designed to last for many years with a minimum of mainte-
nance. It is built to high optical and mechanical standards and to withstand daily use.
We remind you that this manual contains important information on safety and maintenance, and that it must
therefore be made accessible to the instrument users.
We decline any responsibility deriving from incorrect instrument use uses that does not comply with this manual.
Symbols and conventions
The following chart is an illustrated glossary of the symbols that are used in this manual.
CAUTION
This symbol indicates a potential risk and alerts you to proceed with caution.
ELECTRICAL SHOCK
This symbol indicates a risk of electrical shock.
Safety Information
Avoiding Electrical Shock
Before plugging in the power supply, make sure that the supplying voltage of your region matches with the op-
eration voltage of the equipment and that the lamp switch is in off position.
Users should observe all safety regulations of the region. The equipment has acquired the CE safety label.
However, users have full responsibility to use this equipment safely.
Please follow the guidelines below, and read this manual in its entirety to ensure safe operation of the unit.
Intended use
For research and teaching use only. Not intended for any animal or human therapeutic or diagnostic use.
Package Contents
DESCRIPTION QUANTITY
Microscope stand with head, focus and rotating stage 1
WF10x/20 eyepiece 2
Bertrand lens attachment 1
Objective E-PLAN IOS POL 4x 1
Objective E-PLAN IOS POL 10x 1
Objective E-PLAN IOS POL 40x 1
Objective E-PLAN IOS POL 60x 1
Condenser with rotating polarizer 1
Retardation plates (lambda, lambda/4, quartz wedge) 3
Allen wrench for objective centering 2
Power supply 6Vdc 1
Dust cover 1
Page 4
Unpacking
The microscope is housed in a moulded Styrofoam container. Remove the tape
from the edge of the container and lift the top half of the container. Take some
care to avoid that the optical items (objectives and eyepieces) fall out and get
damaged. Using both hands (one around the arm and one around the base), lift
the microscope from the container and put it on a stable desk.
1. First of all remove all the components from the original package and store
the box in a safe and dry place.
2. Place the main body of the microscope on a flat and sturdy surface.
Don’t connect the power supply cable at this stage.
3. Place the attachment with analyzer /Bertrand Lens onto the main body and
turn the head locking screw (1).
4. Insert the optical head and lock the screw.
5. After removing the protection caps, fully insert both eyepieces.
Page 5
Product specications
Head Trinocular, 30° inclined, 360° rotating.
Eyepieces WF10X/20mm with cross hair.
Bertrand lens Swing-out type; centrable.
Polarizing
attachment 0°-90° rotating analyzer filter. Tint plates included: 1° order red (λ), λ/4, quartz wedge.
Nosepiece 4-positions with centering mechanism for all objectives.
Objectives E-PLAN IOS POL (Strain-free) 4x/0.10, 10x/0.25, 40x/0.65, 60x/0.80.
Focusing system Coaxial coarse and fine.
Stage 160mm dia.; 360° rotating with stop knob and 0.1° Vernier.
Condenser N.A. 1.25, with iris diaphragm, focusable and centrable. With rotating polarizer filter.
Illumination X-LED3, with manual brightness control.
Power supply External power supply: Input 100-240Vac 50-60Hz / Output 6Vdc 1A
Overview
6. Connect the external power supply cable to the socket on the
back of the microscope.
If there is no need to install additional accessories,
the instrument is now ready for use.
BERTRAND LENS
CENTERING SCREWS (10)
Page 6
PHOTO/VIDEO PORT
ROTATING STAGE
CONDENSER
CENTERING
SCREWS (2)
POLARIZER
OBJECTIVE
HOLES FOR NOSEPIECE
CENTERING (7)
ANALYZER
ROTATION
SCALE (8)
ANALYZER
INSERTION
LEVER (12)
BERTRAND LENS (13)
RETARDATION PLATE (11)
STAGE ROTATION
LOCK SCREW
EYEPIECES (ONE
WITH CROSSLINE)
DIOPTRIC
ADJUSTMENT
RING
Page 7
Using the microscope
Observation head
Loosen the lock-screw, turn the observation head to a comfortable position for observation, and then lock the
lock-screw.
Place the specimen on the stage
Lock the specimen slide on the mechanical stage using the slide clamp. Ensure that the specimen is centred
over the stage opening by adjusting the coaxial knobs of the stage.
Illumination system settings
The microscope is tted with a white LED illuminator. Turn the brightness adjustment knob to a brightness
suitable for observation.
Adjust interpupillary distance
Hold the right and left parts of the observation head with both hands
and adjust the interpupillary distance by turning the two parts until
one circle of light can be seen.
The white dot (°) placed on the right eyepiece shows the set
interpupillary distance. Just remember this value to help on later
settings.
Focus tension adjustment
The tension of the coarse focusing knob is preset by factory.
To change the tension according to your preference, just rotate the
knob (3) clockwise in order to increase it.
Excessive tension could damage the mechanism of focus. A too
loosed tension causes the descent of the stage by gravity or a
sudden loss of focus. In this case, rotate the knob (3) to increase
the tension.
Diopter adjustment
Turn the dioptric adjustment ring on the left eyepiece halfway. Turn
the coarse focus knob in order to focus the slide with an objective
with low magnication. Adjust the ne focus knob until you obtain
a clear and dened picture observing with the right eye, and then
repeat the operation with the left dioptric compensation ring and the
left eye. When the image appears in focus, choose the necessary
objective with the revolving nosepiece.
TENSION
KNOB (3)
COARSE FOCUS FINE FOCUS
Page 8
Condenser
The condenser, placed below the stage, is equipped with the following features:
Raise or lower the condenser through the knob (5) to obtain a clear and uniform illumination of the sample.
To center the condenser: completely close the iris diaphragm (4). Using the condenser centering screws (2),
move the diaphragm in the center of the eld of view. Then gradually expand the diaphragm until it is tangent to
the edges of the eld of view. If necessary, you can perform an additional adjustment.
The condenser is centered when the edges of iris diaphragm are tangent to the eld of view.
In the bottom part of the condenser there is a polarizer lter, which can be rotated through 360°, with a reference
notch for a precise angle measurement.
APERTURE DIAPHRAGM
ADJUSTMENT RING (4)
ROTATING POLARIZER WITH
ANGULAR SCALE (9)
Page 9
Numerical aperture setting
The value of the numerical aperture (N.A.) of the diaphragm is an indication of the contrast of the illumination
system. Matching the value of illumination system’s N.A. with that of the objective ensures the best results in
terms of contrast and image quality.
To set the numerical aperture of the illuminator, adjust the opening of the iris diaphragm (4). In this way you con-
trol contrast and image resolution.
For samples with low contrast set the iris to about 75% of the value of the objective’s numerical aperture.
System centering
In order to correctly center the whole optical system, rst remove the Bertrand lens out of the path by rotating its
disc. Remove also the lower polarizer lter.
The rotating stage is factory pre-centered and so it doesn’t need any additional operation.
It’s necessary to center the objectives following this procedure:
1. Place a specimen on the stage.
2. Insert 10x objective.
3. Insert the two little wrenches into the holes for nosepiece centering (7).
4. Focus your sample while continuously rotating the stage clockwise and then counter-clockwise by a little
angle (e.g. 30° or 45°). During these oscillations you should spot a point on the sample that doesn’t rotate
on a circumference but only revolving about itself (xed point). With the two objective’s centering wrenches,
bring this point in the center of the crossline.
5. Insert the 20x objective.
6. Insert the two little wrenches into the holes for nosepiece centering (7), above the 20x objective.
7. Rotate the two wrenches until the same point of the sample is brought in the center of the crossline.
8. Repeat the operation for the other objectives.
HEIGHT ADJUSTMENT FOR
CONDENSER (5)
Page 10
Polarized light observation
In order to correctly use the polarized light, it’s necessary to perform a setting for “light extinction”:
1. Remove from the optical path the sample, retardation plates, etc...
2. Insert the 10x objective.
3. Insert in the optical path the analyzer lter and rotate the rotation scale (8) in correspondence to
position “0”.
4. Put the angular scale (9) of the polarizer lter to “0” position.
5. Loosen the lock screw of the polarizer and, looking into the eyepieces, rotate the scale of the polarizer until
you obtain the complete extinction of the light (totally dark).
6. Then lock the polarizer screw.
Bertrand lens
Bertrand lens is employed in conoscopic observation mode (lens inserted) or orthoscopic mode (lens not in-
serted).
Orthoscopic observation
The path of the polarized light is parallel to the optical axis, thus allowing the observation of the optical charac-
teristics of the specimen. You can use objectives from 4x to 100x.
1. Remove the Bertrand lens out from optical path.
2. Insert the analyzer lter using the proper lever (12) and look at the sample.
3. Insert a retardation plate (11) pushing it completely inside the slot under the head.
To remove the plate from the optical path, pull it outward till the rst click.
Conoscopic observation
1. Use objectives from 20X to 100X.
2. Insert the analyzer using the proper lever (12) and put it in the extinction position.
3. Insert the Bertrand lens.
4. Insert the objective of choice (from 20X to 100X).
5. Open the aperture diaphragm.
6. It’s possible to center the conoscopic image adjusting the screws (10) placed under of the head.
In this manner it’s possible to look at the conoscopic images that form on the back focal plane of the objective. If
the peripheral part of the image is too dark, you can just move the condenser vertically to nd the optimal posi-
tion for the illlumination.
Retardation plates
Retardation plates (1 lambda, corresponding to 530nm or Red 1° order, ¼ lambda corresponding to 137nm, and
quartz wedge) are used to make the polarization analysis more precise and rened.
Insert or remove the plate in the optical path of the microscope by pushing it into the slot (11) under the head.
Retardation plates have no visible effect when used in normal brighteld light.
IMAGE CONOSCOPIC
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