Optika Italy B-1000 Series User manual
Ver. 2.0 2019
Model
B-1000FL-LED
B-1000 Series
INSTRUCTION MANUAL
Page 2
Table of Contents
Warning
Symbols and conventions
Safety Information
Intended use
List of accessories and spare parts
Overview
Unpacking
Assembling
Using the microscope
Maintenance
Troubleshooting
Equipment disposal
Page 3
Warning
This microscope is a scientic precision instrument designed to last for many years with a minimum of mainte-
nance. It is built to high optical and mechanical standards and to withstand daily use. We remind you that this
manual contains important information on safety and maintenance, and that it must therefore be made acces-
sible to the instrument users. We decline any responsibility deriving from incorrect instrument use uses that does
not comply with this manual.
Symbols and conventions
The following chart is an illustrated glossary of the symbols that are used in this manual.
CAUTION
This symbol indicates a potential risk and alerts you to proceed with caution.
ELECTRICAL SHOCK
This symbol indicates a risk of electrical shock.
Safety Information
Avoiding Electrical Shock
Before plugging in the power supply, make sure that the supplying voltage of your region matches with the
operation voltage of the equipment and that the lamp switch is in off position. Users should observe all safety
regulations of the region. The equipment has acquired the CE safety label. However, users have full responsibil-
ity to use this equipment safely. Please follow the guidelines below, and read this manual in its entirety to ensure
safe operation of the unit.
Intended use
Standard models
For research and teaching use only. Not intended for any animal or human therapeutic or diagnostic use.
IVD Models
Also for diagnostic use, aimed at obtaining information on the physiological or pathological situation of the
subject.
Page 4
List of accessories and spare parts
CAT. NO. DESCRIPTION
M-005 Micrometric slide, 26x76mm, range 1mm, div. 0,01mm
M-613 Polarizing set (lters only).
M-615 Lambda lter for polarizing set.
M-617.1N Phase contrast set with IOS PLAN objective 40x.
M-690 Eyecup (pair).
M-619 Photo tube adapter for full frame SLR camera.
M-173 Photo adapter for APS-C and Full Frame Reex cameras.
M-620 C-Mount adapter for 1/3” sensor.
M-620.1 C-Mount adapter for 1/2” sensor.
M-699 Universal adapter for OPTIKAM & DIGI cameras (only models with eyepiece adapter,
23mm).
M-114 C-Mount adapter for 1/2” sensor.
M-116 C-Mount adapter for 2/3” sensor.
M-113.1 Ring adapter, 30mm (for monocular and binocular microscopes).
15008 OPTIKA immersion oil, 10ml.
Page 5
Overview
COARSE
FOCUSING
KNOB
NOSEPIECE
DIOPTRIC
ADJUSTMENT
RING
PHOTO PORT
MAIN ON/OFF
EYEPIECE
FILTER
SELECTOR
LEVER
CONDENSER
CENTERING
KNOBS
APERTURE
DIAPHRAGM
FIELD DIAPHRAGM
BRIGHTNESS
ADJUSTMENT KNOB
FOCUS-STOP KNOB
FINE FOCUSING
KNOB
OBJECTIVE
STAGE
LED FLUORESCENCE
Page 6
Put the microscope stand on a
solid table.
First insert the LED at-
tachment, use the 2mm Allen
wrench to tighten the screw.
All screws are already inser-
ted into each threaded hole.
(Fig.1)
Unpacking
The microscope is housed in a moulded Styrofoam container. Remove the tape from the edge of the container
and lift the top half of the container. Take some care to avoid that the optical items (objectives and eyepieces)
fall out and get damaged. Using both hands (one around the arm and one around the base), lift the microscope
from the container and put it on a stable desk.
Assembling
Once you open the box, these are the microscope’s components:
① Condenser
② Optical head
③ Main body
④ Eyepieces
⑤ Power supply (quantity: 2)
⑥ Stage
⑦ Objectives
⑧ LED attachment
⑨ Black slide
①
②
③
④
⑤
⑥
⑦
⑧
⑨
Fig.1
Page 7
Insert the condenser under
the stage: position until it is
well inserted into its holder
(under the condenser there is
a pin that must fully enter the
guide of the holder). (Fig. 4)
AFTER INSERTING THE
CONDENSER, TIGHTEN
THIS SCREW
Insert the optical head above
the attachment, using the
2mm Allen wrench to tighten
the screw. (Fig.2)
Insert both eyepieces into
the tubes of the optical head.
(Fig.3)
Mount the stage: lower the
support using the coarse
focus knob, then place the
stage and rmly tighten the
lock screw. (Fig.5)
Fig.2
Fig.3
Fig.4
Fig.5
Page 8
Insert the power supply jack
on the connector at the rear:
one for brighteld illumination,
one for illumination. (Fig.7)
Screw each objective into the
thread of the nosepiece, in
order of magnication. (Fig.6)
Fig.6
Fig.7
Page 9
B-1000 illumination can be managed through
the keyboard placed on the left of the stand:
1) ON-OFF: press this button to turn on or off
the LED illuminator.
2) BOOST: press this button in order to incre-
ase the brightness (useful for high-magnica-
tion objectives or very opaque specimens).
3) AUTO-OFF: if you want the illuminator to
switch off automatically, press this button until
15, 30 or 60 minutes delay is set. After this
period of time, the light will turn off. You have
to press the ON-OFF button to turn it on again.
(Fig.8)
In order to activate the transmitted light illu-
minator, insert the plug of the external power
supply into the socket and turn on the main
switch on the side of the main body. Press
the ON-OFF button on the control keyboard
and turn the brightness adjustment knob to a
brightness suitable for observation. (Fig.9)
Loosen the lock-screw, turn
the observation head to
a comfortable position for
observation, and then lock the
lock-screw. (Fig.10)
Do not enable boost mode while observing with
low magnication objectives (4x, 10x) with fully
open diaphragm: the high brightness may hurt
user’s eyes.
Control keyboard
LED settings
Adjust the observation
head
1) ON-OFF
3) AUTO-OFF
2) BOOST
MAIN
SWITCH
Using the microscope
Fig.8
Fig.9
Fig.10
Page 10
Hold the right and left parts of
the observation head using
both hands and adjust the
interpupillary distance by
turning the two parts until one
circle of light can be seen.
(Fig.11)
Adjust interpupillary
distance
Fig.11
Place the specimen on
the stage
Focus tension
adjustment
Fix the specimen slide on the
mechanical stage using the
slide-clamp. Ensure that the
specimen is centred over the
stage opening. (Fig.12)
Turn the tension-adjust knob
to get a suitable tension for
the focus system. (Fig.13)
NOTE: if the tension is too lo-
ose, the stage could go lower
by itself or the focus easily
lost after ne adjustment. In
this case, rotate the knob in
order to increase the friction.
Fig.12
Fig.13
Focus-stop knob
Loosen the focus-stop knob,
rotate the coarse focusing
knob to bring the slide into
focus with a 4X or 10X objecti-
ve. Then rotate the focus-stop
in order to block the height of
the stage. This simplies the
next focusing operations.
The focus-stop knob is also
useful to avoid accidental con-
tacts between objective and
specimen. (Fig.14) Fig.14
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