Optika Italy IM Series User manual

Ver. 1.0 2023

Model

IM-300

IM Series

INSTRUCTION MANUAL

Page 2

Table of Contents

1. Warning 3

2. Safety Information 3

3. Package content 4

4. Unpacking 5

5. Intended use 5

6. Symbols and conventions 5

7. Instrument description 6

8. Assembling 8

8.1 Installing the objectives 8

8.2 Installing stage extension or mechanical stage 8

8.3 Installing the stage insert 9

8.4 Installing the eyepieces 9

8.5 Installingcolorlters 9

8.6 Installinglterslider 9

8.7 Connecting the power supply 10

9. Brightfield observation 11

10. Use of the microscope in brightfield 12

10.1 Turning on the microscope 12

10.2 Adjusting the light intensity 12

10.3 Adjusting the coarse focus tension 12

10.4 Diopter adjustment 12

10.5 Adjusting interpupillary distance 13

10.6 Use of eyeshields 13

10.7 Selecting the light path 13

10.8 Stage and stage inserts 14

10.8.1 Installing stage inserts 15

10.9 Aperture diaphragm 15

10.10Usingcolorlters 16

11. Use of the microscope in phase contrast 17

11.1 Installing the phase contrast slider 17

11.2 Phase contrast slider 17

11.3 Centering the phase ring 17

12. Use of the microscope in RPC 19

12.1 Installing the RPC slider 19

12.2 RPC slider 19

12.3 RPC observation 20

13. Microphotography 21

13.1 Use of C-mount cameras 21

13.2 UseofReexcameras 21

14. Maintenance 22

15. Troubleshooting 23

Equipment disposal 25

Page 3

1. Warning

This microscope is a scientic precision instrument designed to last for many years with a minimum of maintenance. It is

built to high optical and mechanical standards and to withstand daily use. We remind you that this manual contains im-

portant information on safety and maintenance, and that it must therefore be made accessible to the instrument users. We

decline any responsibility deriving from incorrect instrument use uses that does not comply with this manual.

2. Safety Information

Avoiding Electrical Shock

Before plugging in the power supply, make sure that the supplying voltage of your region matches with the operation voltage

of the equipment and that the lamp switch is in o󰀨 position. Users should observe all safety regulations of the region. The

equipment has acquired the CE safety label. However, users have full responsibility to use this equipment safely. Please

follow the guidelines below, and read this manual in its entirety to ensure safe operation of the unit.

Page 4

① Microscopebody

② Eyepieces

③ Phasecontrastslider

④ Filterholderslider

⑤ BluelterLBD

⑥ GreenlterIF550

⑦ Metalinsertforstage

⑧ Glassinsertforstage

⑨ Objectives

⑩ Dustcover

⑪ Cleaningtissue

⑫ Centeringtelescope

⑬ Powersupply

⑪

⑫

⑩

③

④ ⑤ ⑥

⑦ ⑧

⑨

①

②

3. Package content

⑬

Page 5

4. Unpacking

The microscope is housed in a moulded Styrofoam container. Remove the tape from the edge of the container and lift the

top half of the container. Take some care to avoid that the optical items (objectives and eyepieces) fall out and get damaged.

Using both hands (one around the arm and one around the base), lift the microscope from the container and put it on a

stable desk.

5. Intended use

Standard models

For research and teaching use only. Not intended for any animal or human therapeutic or diagnostic use.

IVD Models

Also for diagnostic use, aimed at obtaining information on the physiological or pathological situation of the subject.

6. Symbols and conventions

The following chart is an illustrated glossary of the symbols that are used in this manual.

CAUTION

This symbol indicates a potential risk and alerts you to proceed with caution.

ELECTRICAL SHOCK

This symbol indicates a risk of electrical shock.

Page 6

FILTER HOLDER

DIOPTER

ADJUSTMENT

RING CONDENSER

COARSE

FOCUS KNOB

FINE FOCUS

KNOB

TENSION

ADJUSTMENT

COLLAR

OBJECTIVE

MICROSCOPE

BODY

7. Instrument description

BRIGHTNESS

CONTROL KNOB

Page 7

PHASE

CONTRAST

SLIDER

PHOTO

PORT

EYEPIECE

COARSE

FOCUS KNOB

STAGE LIGHT PATH

SELECTOR

LEVER

STAGE

PLATE

MAIN SWITCH

Opposite side

LED

HOUSING

APERTURE

DIAPHRAGM

Page 8

8. Assembling

FFig. 1ig. 1

①

②

FFig. 2ig. 2

FFig. 3ig. 3

③

FFig. 4ig. 4

8.1 Installing the objectives

1. Turn the coarse focusing knob ① until the nosepiece reaches

its lowest position.

• For a safe transport, the nosepiece is placed in the low-

est position and the tension adjustment collar ②is ad-

justed to the proper tension when the microscope leaves

the factory. (Fig.1)

2. Screw the lowest magnication objective on the nosepiece

from the right side, then turn the nosepiece clockwise. Mount

the other objectives in the same way, following the sequence

from low to high.

• Note: the objectives can also be installed through the

stage opening. (Fig. 2)

• Clean the objectives regularly. In inverted microscopes, the

objectives are very sensitive to dust.

• To prevent dust and contamination from entering the micro-

scope, cover all the unused holes with dust caps ③. (Fig. 3)

• When operating, use a low power objective to search and

focus the specimen, then switch to higher magnications.

• When switching between objectives, slowly turn the nose-

piece until it clicks. The click means that the objective is in

the right position, in the center of the light path.

8.2 Installing stage extension or mechanical stage

• Stage extension and mechanical stage are optional ac-

cessories for some models.

• Stage extension can be installed on either side of the stage

to enlarge the working surface.

• Mechanical stage can only be installed on the right side.

1. Installing the units: screw the bolts in the xing holes of the

stage, then mount the unit from below the stage. (Fig. 4)

• NOTE: The stage has a series of holes in the underside.

To install the mechanical stage it is necessary, starting

counting from the front of the microscope, to use the

thirdandfthholes.Byusingadi󰀨erentsetofholes,the

mechanical stage will not be installed correctly.

Page 9

FFig. 5ig. 5

FFig. 6ig. 6

FFig. 7ig. 7

8.3 Installing the stage insert

• Install the glass or metal plate according to individual pref-

erences.

Install the stage insert in the stage opening. (Fig. 5)

FFig. 8ig. 8

8.4 Installing the eyepieces

Insert both eyepieces into the tubes of the optical head. (Fig. 6)

8.5 Installingcolorlters

1. Place the lter slider ① on the table and insert the desired

colored lter into one of the two empty positions ②. (Fig. 7)

• Takecarethatthelterispositionedhorizontallyinthe

slider to prevent it from getting stuck during movement.

8.6 Installinglterslider

1. Insert the lter slider into the upper slot of the condenser ①

with the grooves ② facing the rear of the microscope. (Fig. 8)

• The slider has two positions to accommodate two col-

oredlters. Move theslider tothe positioncontaining

thedesiredlteruntilitclicksintoplace. ①

②

Page 10

8.7 Connecting the power supply

1. Insert the plug of the power supply into the socket ①at the

rear of the instrument. (Fig. 9)

2. Plug the power supply into the wall socket.

FFig. 9ig. 9

①

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