Molecular Devices MultiWash+ User manual

5032022 C

August 2019

MultiWash+ Microplate Washer

User Guide

MicroWash+ Microplate Washer User Guide

2 5032022 C

This document is provided to customers who have purchased Molecular Devices equipment, software,

reagents, and consumables to use in the operation of such Molecular Devices equipment, software,

reagents, and consumables. This document is copyright protected and any reproduction of this

document, in whole or any part, is strictly prohibited, except as Molecular Devices may authorize in

writing.

Software that may be described in this document is furnished under a non-transferrable license. It is

against the law to copy, modify, or distribute the software on any medium, except as specifically

allowed in the license agreement. Furthermore, the license agreement may prohibit the software

from being disassembled, reverse engineered, or decompiled for any purpose.

Portions of this document may make reference to other manufacturers and/or their products, which

may contain parts whose names are registered as trademarks and/or function as trademarks of their

respective owners. Any such usage is intended only to designate those manufacturers’ products as

supplied by Molecular Devices for incorporation into its equipment and does not imply any right and/or

license to use or permit others to use such manufacturers’ and/or their product names as trademarks.

Each product is shipped with documentation stating specifications and other technical information.

Molecular Devices products are warranted to meet the stated specifications. Molecular Devices makes

no other warranties or representations express or implied, including but not limited to, the fitness of

this product for any particular purpose and assumes no responsibility or contingent liability, including

indirect or consequential damages, for any use to which the purchaser may put the equipment

described herein, or for any adverse circumstances arising therefrom. The sole obligation of Molecular

Devices and the customer's sole remedy are limited to repair or replacement of the product in the

event that the product fails to do as warranted.

For research use only. Not for use in diagnostic procedures.

The trademarks mentioned herein are the property of Molecular Devices, LLC or their respective owners. These trademarks may not

be used in any type of promotion or advertising without the prior written permission of Molecular Devices, LLC.

Patents: http://www.moleculardevices.com/patents

Product manufactured by Molecular Devices, LLC.

3860 N. First Street, San Jose, California, 95134, United States of America.

Molecular Devices, LLC is ISO 9001 registered.

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Contents

Safety Information 5

Warnings, Cautions, Notes, and Tips 5

Chapter 1: Unpacking and Setting Up the Instrument 9

Contents of the Package 10

Unpacking the Washer 11

Installing the Manifold 15

Connecting the Fluid Bottles 17

Installing the Microplate Carrier 18

Connecting Power to the Instrument 19

Chapter 2: Using the Instrument 21

Using the Instrument Control Panel 22

Software Overview 23

Setting Up the Software 24

Loading a Microplate 24

Chapter 3: Running a Procedure 25

Running the Selected Procedure 26

Chapter 4: Priming or Rinsing the Fluidics System 31

Running a Manual Prime or Rinse Process 32

Running an Automatic Prime or Rinse Process 37

Running a Periodic Prime or Rinse Process 43

Chapter 5: Defining a Procedure 51

Defining the Procedure 52

Chapter 6: Defining a Wash or Rinse Cycle 65

Defining the Cycle 66

Chapter 7: Defining a Microplate Type 77

Defining Microplate Types 78

Defining a Round-Bottom Microplate Type 80

Defining a Flat-Bottom Microplate Type 95

Chapter 8: Selecting Instrument Set Up Options 113

Selecting the Language for the Display 114

MicroWash+ Microplate Washer User Guide

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Selecting the Installed Manifold 115

Selecting the Number of Installed Wash Bottles 116

Scrolling Through the Detection Options 117

Selecting the Display of the Liquid Level Warning 118

Selecting the Display of the Waste Level Warning 119

Selecting the Display of the Microplate Warning 120

Selecting the Key Click Option 121

Selecting the Emergency Stop Option 122

Chapter 9: Maintenance and Troubleshooting 123

Preventive Maintenance 123

Clean the Instrument 124

Maintaining the Fluidics System and Manifold 126

Replacing Fuses 130

Packing the Instrument for Storage or Service 132

Status Messages and Error Messages 141

Obtaining Support 142

Appendix A: Parts and Accessories 143

Appendix B: Instrument Specifications 145

Physical Specifications 145

Measurement Specifications 146

Appendix C: System Dimensions 147

Index 149

Safety Information

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Safety Information

Information about the safe use of the instrument from Molecular Devices® includes an

understanding of the user-attention statements in this guide, the safety labels on the

instrument, precautions to follow before you operate the instrument, and precautions to

follow while you operate the instrument.

Make sure that everyone involved with the operation of the instrument has:

Received instruction in general safety practices for laboratories.

Received instruction in specific safety practices for the instrument.

Read and understood all Safety Data Sheets (SDS) for all materials being used.

Read and observe all warnings, cautions, and instructions. The most important key to safety

is to operate the instrument with care.

WARNING! If the instrument is used in a manner not specified by Molecular

Devices, the protection provided by the equipment might be impaired.

Warnings, Cautions, Notes, and Tips

All warning symbols are framed within a yellow triangle. An exclamation mark is used for

most warnings. Other symbols can warn of other types of hazards such as biohazard,

electrical, or laser safety warnings as are described in the text of the warning. Follow the

related safety information.

The following user attention statements might be displayed in the text of Molecular Devices

user documentation. Each statement implies the amount of observation or recommended

procedure.

WARNING! A warning indicates a situation or operation that could cause

personal injury if precautions are not followed.

CAUTION! A caution indicates a situation or operation that could cause damage to

the instrument or loss of data if correct procedures are not followed.

Note: A note calls attention to significant information.

Tip: A tip provides useful information or a shortcut, but is not essential to the

completion of a procedure.

MicroWash+ Microplate Washer User Guide

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Symbols on the Instrument

Each safety label found on the instrument contains an alert symbol that indicates the type of

potential safety hazard.

Symbol Indication

Consult the product documentation.

Potential biohazard.

Power switch indicates power on.

Power switch indicates power off.

Indicates the location of the Protective Ground Terminal.

Required in accordance with the Waste Electrical and Electronic Equipment

(WEEE) Directive of the European Union. It indicates that you must not discard this

electrical or electronic product or its components in domestic household waste or

in the municipal waste collection system.

For products under the requirement of the WEEE directive, contact your dealer or

local Molecular Devices office for the procedures to facilitate the proper

collection, treatment, recovery, recycling, and safe disposal of the device.

- California proposition 65 requires businesses to provide

warnings to Californians about significant exposures to chemicals that cause cancer, birth

defects, or other reproductive harm.

Safety Information

5032022 C 7

Service Trained Users

Most procedures required to operate and troubleshoot the instrument can be done by a

user who reads the instructions in this user guide and is familiar with the system. However,

all installation procedures and some complex service and troubleshooting procedures

require the expertise of a service trained user. When Molecular Devices installs the

instrument, part of the setup procedure is to provide training for the people in your

company who are to be your company's service trained users. Contact Molecular Devices to

arrange additional training.

Whenever the following warning message appears, a service trained user must do the

procedure to ensure user safety and to prevent instrument damage. Example:

WARNING! The following procedures must be completed by a service trained

user. Do not attempt the following procedures if you have not received proper

training by Molecular Devices personnel.

Electrical Safety

To prevent electrical injuries and property damage, inspect all electrical equipment before

use and report all electrical deficiencies. Contact Molecular Devices technical support for

equipment service that requires the removal of covers or panels.

To prevent electrical shock, use the supplied power cord and connect to a properly

grounded wall outlet.

To ensure sufficient ventilation and provide access to disconnect power from the

instrument, maintain a 20cm to 30cm (7.9in. to 11.8in.) gap between the rear of the

instrument and the wall.

Power off the instrument when not in use.

Chemical and Biological Safety

Normal operation of the instrument can involve the use of materials that are toxic,

flammable, or otherwise biologically harmful. When you use such materials, observe the

following precautions:

Handle infectious samples based on good laboratory procedures and methods to

prevent the spread of disease.

Observe all cautionary information printed on the original containers of solutions before

their use.

Dispose of all waste solutions based on the waste disposal procedures of your facility.

Operate the instrument in accordance with the instructions outlined in this guide, and

take all the required precautions when using pathological, toxic, or radioactive materials.

MicroWash+ Microplate Washer User Guide

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Splashing of liquids can occur. Take applicable safety precautions, such as using safety

glasses and wearing protective clothing, when working with potentially hazardous

liquids.

Observe the applicable cautionary procedures as defined by your safety officer when

using hazardous materials, flammable solvents, toxic, pathological, or radioactive

materials in or near a powered-up instrument.

WARNING! Never use the instrument in an environment where potentially

damaging liquids or gases are present.

Moving Parts Safety

The instrument contains moving parts that can cause injury. Under normal conditions, the

instrument is designed to protect you from these moving parts.

WARNING! If the instrument is used in a manner not specified by Molecular

Devices, the protection provided by the equipment might be impaired.

To prevent injury:

Never try to exchange labware, reagents, or tools while the instrument is operating.

Never try to physically restrict the moving components of the instrument.

Keep the instrument work area clear to prevent obstruction of the movement.

Always keep the cover closed when the instrument is in use.

Note: Observe all warnings and cautions listed for all external devices attached to or in

use during the operation of the instrument. See the applicable user guide for the

operating and safety procedures of that device.

5032022 C 9

Chapter 1: Unpacking and Setting Up the Instrument

Before unpacking and setting up the MultiWash+ Microplate Washer, prepare a dry, flat,

vibration-free work area that has sufficient space for the instrument, the fluidics bottles and

tubing, and the power cable.

The instrument is 21.00cm (8.27in.) wide, 21.00cm (8.27in.) high, and 46.00cm (18.11in.)

deep. To provide access to disconnect power from the instrument, maintain a 20cm to

30cm (7.9in. to 11.8in.) gap between the rear of the instrument and the wall.

Keep the instrument away from direct sunlight.

Unpacking and setting up the instrument includes the following procedures:

Unpacking the instrument and saving the original packaging. See Unpacking the Washer

on page 11.

Installing the manifold and connecting the fluidics tubing. See Installing the Manifold on

page 15.

Connecting the fluidics tubing to the waste, rinse, and wash bottles. See Connecting the

Fluid Bottles on page 17.

Installing the microplate carrier on the instrument deck. See Installing the Microplate

Carrier on page 18.

Connecting the power cord and powering on the instrument. See Connecting Power to

the Instrument on page 19.

MicroWash+ Microplate Washer User Guide

10 5032022 C

Contents of the Package

The package contains the instrument and the accessories that are required for installing the

instrument as follows:

MultiWash+ Microplate Washer Quick-Start Guide

MultiWash+ Microplate Washer

Power cord

2.5L waste bottle

2.0L rinse bottle

2.0L wash bottles (3)

Microplate carrier

8-channel manifold

Fluidics tubing

Cleaning tools for manifold needles

Spare fuses (2)

Figure 1-1: Instrument and Accessories

Table of contents

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