Leica DM4000B User manual
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Leica DM4000 B
Leica DM4000 M
Leica DM5000 B
Operating Manual
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Published 2003 by:
Leica Microsystems Wetzlar GmbH
Ernst-Leitz-Straße
D-35578 Wetzlar (Germany)
Responsible for contents:
Katja Peter, Karin Schwab
Marketing CM, Compound Microscopy, Product Management Phone +49(0)6441-292261
Fax +49(0)6441-292255
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Leica DM4000 B
Leica DM4000 M
Leica DM5000 B
Operating Manual
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Copyrights
Copyrights
All rights to this documentation are held by Leica
Microsystems Wetzlar GmbH. Reproduction of
text or illustrations (in whole or in part) by print,
photocopy, microfilm or other methods (includ-
ing electronic systems) is not allowed without
express written permission from Leica
Microsystems Wetzlar GmbH.
The term "Windows" can be used in the following
text without further identification. It is a
registered trademark of the Microsoft
Corporation. Otherwise, no inference with
regard to the free usability of product names
may be drawn from the use of those names.
The instructions contained in the following docu-
mentation reflect state-of-the-art techno-logy
and knowledge standards. We have compiled
the texts and illustrations as accurately as
possible. Nevertheless, no liability of any kind
may be assumed for the accuracy of this manu-
al’s contents. Still, we are always grateful for
comments and suggestions regarding potential
mistakes within this documentation.
The information in this manual is subject to modifi-
cation at any time and without notification.
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Contents
6. Startup ........................................................ 31
6.1 Functional Principle.................................. 31
6.2 Switching on the Microscope ................ 34
6.3 The Display
(Leica DM4000 B/DM4000 M) ................. 35
6.4 The Function Keys .................................... 36
6.5 Köhler Illumination .................................... 37
6.6. Checking Phase Contrast Rings ............. 39
6.7 Adjusting the Light Sources .................... 40
7. Operation ................................................... 46
7.1 Switching on the Microscope ................ 46
7.2 Stages and Specimen Displacement .... 46
7.3 Focusing ..................................................... 47
7.4 Tubes...........................................................
48
7.5 Eyepieces ................................................... 49
7.6 Objectives .................................................. 50
7.7 Magnification Changer ............................ 51
7.8 Light Sources ............................................. 52
7.9 Aperture Diaphragm and
Field Diaphragm ........................................ 52
8. Imaging Procedure for
Leica DM4000 B/Leica DM5000 B ......... 53
8.1 Transmitted Light ...................................... 53
8.1.1 Bright Field ...................................... 53
8.1.2 Phase Contrast ............................... 53
8.1.3 Dark Field ......................................... 54
8.1.4 Polarization ..................................... 55
8.1.5 Differential
Interference Contrast .................... 56
Contents
1. Important Notes about this Manual ..... 7
2. Safety Notes .............................................. 8
2.1. General Safety Notes ............................... 8
2.2. Electrical Safety ........................................ 8
3. Overview of the Instrument .................... 10
4. Unpacking the Microscope .................... 14
5. Assembling the Microscope .................. 16
5.1 Stage ........................................................... 17
5.2 Condenser .................................................. 18
5.3 Tube and Eyepieces ................................. 19
5.4 Objectives .................................................. 19
5.5 Light Sources for the
Transmitted Light Axis ............................. 20
5.6 Light Sources for the
Incident Light Axis .................................... 21
5.7 Equipping the
Incident Light filter turret ........................ 26
5.8 Polarizer and Analyzer ............................. 27
5.9 DIC Prisms .................................................. 28
5.10 Optional Accessories ............................... 29
5.11 Connection to the Power Supply............ 30
5.12 Connection to the
CTR5000 Electronics Box ......................... 30
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Contents
8.2 Fluorescence ............................................. 57
9. Imaging Procedure for
Leica DM4000 M ....................................... 58
9.1 Incident Light ............................................. 58
9.1.1 Bright Field ...................................... 58
9.1.2 Dark Field......................................... 58
9.1.3 Polarization ..................................... 59
9.1.4 Interference Contrast .................... 60
9.2 Transmitted Light ...................................... 60
9.2.1 Bright Field ...................................... 60
10. Trouble Shooting ...................................... 61
11. Care of the Microscope ........................... 64
11.1 Dust Cover .................................................. 64
11.2 Cleaning ...................................................... 64
11.3 Handling Acids and Bases ...................... 65
12. Essential
Wear and Spare Parts ............................. 66
13. Abbreviations and Pictograms .............. 67
14. Index ........................................................... 68
15. EU Declaration of Conformity ................ 69
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1. Important Notes about this Manual
(1.2)
→p. 20
!
*
Numbers in parentheses, such as "(1.2)", corre-
spond to illustrations (in the example, Figure 1,
Item 2).
Numbers with pointer arrows (for example
→ p.20), point to a certain page of this manual.
Special safety instructions are indicated
with the triangle symbol shown here, and
have a gray background.
Caution! The microscope and accessories can
be damaged when operated incorrectly.
Explanatory note.
Item not contained in all configurations.
Text symbols and their meanings:
Caution!
This operating manual is an essential com-
ponent of the microscope, and must be read
carefully before the microscope is put into
operation or used.
1. Important Notes about this Manual
This operating manual contains important in-
structions and information for the operational
safety and maintenance of the microscope and
accessories. Therefore, it must be kept and
taken care of.
8
2. Safety Notes
2. Safety Notes
2.2 Electrical Safety
General specifications
Leica CTR5000 electronics box (for DM5000 B)
For indoor use only.
Supply voltage:
Frequency:
Power input:
Fuses:
Ambient temperature:
Relative humidity:
Overvoltage category:
Pollution degree:
Microscope
For indoor use only.
Supply voltage:
Frequency:
Power input:
DM4000
DM5000
Fuses:
DM4000
DM5000
Ambient temperature:
Relative humidity:
Overvoltage category:
Pollution degree:
2.1 General Safety Notes
This safety class 1 device is constructed and
tested in accordance with EN 61010-1/IEC 1010-1,
safety regulations for electrical measuring, con-
trol, and laboratory devices.
Caution!
In order to maintain this condition and to en-
sure safe operation, the user must follow the
instructions and warnings contained in this
operating manual.
Caution!
The devices and accessories described in
this operating manual have been tested for
safety and potential hazards.
The responsible Leica affiliate or the main
plant in Wetzlar must be consulted when-
ever the device is altered, modified or used
in conjunction with non-Leica components
that are outside of the scope of this manual.
Unauthorized alterations to the device or
noncompliant use shall void all rights to any
warranty claims!
90-250 V~
50-60 Hz
max. 290 VA
T6,3 A
(IEC 60127-2/3)
15-35°C
max. 80% to 30°C
II
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90-250 V~
50-60 Hz
max. 180 VA
max. 290 VA
T6,3 A
(IEC 60127-2/3)
See CTR5000
15-35°C
max. 80% to 30°C
II
2
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2. Safety Notes
Caution!
Never use any fuses as replacements other
than those of the types and the current rat-
ings listed here. Using patched fuses or
bridging the fuse holder is not permitted.
Caution!
The microscope’s electrical accessory com-
ponents are not protected against water.
Water can cause electric shock.
Caution!
Protect the microscope from excessive tem-
perature fluctuations. Such fluctuations can
lead to the accumulation of condensation,
which can damage the electrical and optical
components.
Ambient temperature: 15-35°C.
Caution!
Before exchanging the fuses or lamps, be
absolutely certain to switch-off the main
power switch and remove the power cable.
Supply unit ebq 100
For indoor use only.
Supply voltage:
Frequency:
Power input:
Fuses:
Ambient temperature:
Relative humidity:
Overvoltage category:
Pollution degree:
(see enclosed manual)
90-250 V~
50-60 Hz
max. 155 VA
2xT2A (IEC 127)
15-35°C
max. 80% to 30°C
II
2
Caution!
The power plug may only be plugged into an
outlet equipped with a grounding contact.
Do not interfere with the grounding function
by using an extension cord without a ground
wire. Any interruption of the ground wire in-
side or outside of the device, or release of
the ground wire connection, can cause the
device to become hazardous. Intentional
ground interruption is not permitted!
Caution!
Through connection to the grounding con-
nection, ancillary equipment with its own
and/or extra power supply may be brought to
the same ground wire potential. For
connections without a ground connector,
Leica Service must be consulted.
10
3. Overview of the Instrument
Specification
Imaging Procedure
Transmitted Light Axis
Incident Light Axis
Z Pinion
Objective nosepiece
X/Y Stage
Tube
3. Overview of the Instrument
Leica DM4000 B / DM5000 B
• transmitted light: BF, DF, PH,
Pol (DM5000 B also ICT)
• incident light: fluorescence
• integrated into the stand
• motorized 5x filter turret
(DM5000 B 8x optional)
• with FIM (Fluorescence
Intensity Managemer) for de-
creasing light intensity in 5
stages
• mechanical “Booster Lens”
for increasing fluorescence
intensity
• motorized shutter
• manual
• absolute coded
• 6x with M25 thread
(DM5000 B: 7x; mot. DIC
objective prism turret with 4
positions optional)
• manual
• replaceable specimen stage
• coaxial pinion length: 155 mm
Leica DM4000 M
• transmitted BF, DF, PH
light: ICT, Pol
• incident light: BF, DF, ICR, Pol
• integrated into the stand
• motorized 4x filter turret
• automatic Illumination
Manager
• motorized shutter
• manual
• absolute encoded
• 6x with M32 thread
• slot for DIC prisms
and Pol compensators
(optional)
• manual
• replaceable specimen stage
• coaxial pinion length: 140 mm
• automatic Illumination Manager
(motorized aperture diaphragm and field diaphragm,
motorized intensity control)
• automatic Constant Color Intensity Control (CCIC)
• motorized shutter
• manual
• manual or motorized
• optionally with two camera outputs
This manual suits for next models
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