Leica DM R Series User manual
Leica DM R
Instructions
Leica Microsystems Wetzlar GmbH
Ernst-Leitz-Straße
D-35578 Wetzlar (Germany)
Tel. +49(0)6441-290
Fax +49(0)6441-292599
http://www.leica.com
©Leica Microsystems Wetzlar GmbHzErnst-Leitz-Straße zD-35578 Wetzlar zTel. (06441) 29-0 zFax (06441) 29-2599 Printed on chlorine-free bleached paper.
Order nos. of the editions in: English 933789 zGerman 933789 zFrench 933789 zPart-No. 501-148 Printed in Germany IX/98/FX/K.H.
2
4th edition, issued in 1999 by:
Leica Microsystems Wetzlar GmbH
Ernst-Leitz-Straße
D-35578 Wetzlar (Germany)
Responsible for contents:
Marketing MQM, product management Tel. +49(0)6441-292519
Fax +49(0)6441-292255
3
Leica DM R
Instructions
4
5
Contents
Important notes on this manual....................... 7
Assembly and description
of components..................................................... 8
Assembly/General information......................... 8
Light sources ....................................................... 10
Lamp change. ...................................................... 12
Lamphousings...................................................... 16
Filters and filter magazine................................. 17
Specimen stages and condenser holder ....... 18
Condensers (transmitted light)......................... 21
Incident light components ................................ 26
Polarizers/analysers........................................... 32
Tube optics ........................................................... 35
Tubes ..................................................................... 37
Diapositive overlay, macro device .................. 40
Eyepieces ............................................................. 42
Objective nosepiece and objectives............... 45
Objective labelling .............................................. 47
Operation.............................................................. 53
Basic setting
for transmitted and incident light .................... 53
Filters..................................................................... 56
Focusing, mechanical ........................................ 57
Basic functions of motor focus ........................ 58
Calibration of motor focus................................. 62
Objectives............................................................. 65
Tubes and eyepieces.......................................... 67
Transmitted light illumination ........................... 68
Phase contrast .................................................... 73
Transmitted light darkfield................................. 75
Transmitted light polarization ........................... 77
Transmitted light interference contrast.......... 86
Incident light sources ........................................ 90
Fluorescence ....................................................... 93
IGS and RC ........................................................... 94
Incident light brightfield .................................... 95
Incident light darkfield ....................................... 98
Incident light oblique illumination ................... 98
Incident light interference contrast ................ 99
Incident light polarization.................................. 100
Possible errors .................................................... 101
Diapositive overlay device................................ 102
Macro device....................................................... 103
Linear measurements ........................................ 106
Thickness measurements ................................. 108
TV microscopy..................................................... 109
Care and maintenance ...................................... 111
Wearing and spare parts, tools ....................... 112
Index ..................................................................... 113
EU-Conformity declaration ............................... 114
General specifications
Mains voltage: 100–115 V/230 V, ±10%
(E focus)
90 –250V
(mech.focus)
Frequency: 50– 160 Hz ~
Power consumption: max. 160 W
Use: indoors only
Operating temperature: 10– 36°C
Relative humidity: 0– 80% to 30°C
Overvoltage category: II
Contamination class: 2
6
Transmitted light path*
1Light source (lamphousing not illustrated), 2Filter magazine*, 4-pos., 3Diffusing screen,
4Aperture diaphragm, 5Imaging system of aperture diaphragm, 6Field diaphragm, 7Polarizer*,
8Condenser
Incident light path*
9Light source (lamphousing not illustrated), 10 Filter magazine*, 4-pos.
Diaphragm module with:
11 Aperture diaphragm* or filter and diffusing screen, 12 Field diaphragm, 13 Reflector or filter cube
Imagine light path
14 Objective, 15 Tube optics/Bertrand lens*, 16 Tube, 17 Eyepiece
17
16
14
1
10 9
11
12
13
15
2
34
5
6
7
8
* not part for all outfits
7
Special manuals are supplied with some addi-
tional equipment such as photomicrography,
microscope photometry (MPV), compensators,
heating stages, interference attachments, etc.
There are also extensive brochures on micro-
scopy, which can be ordered, as can extra
copies of this manual, from our agencies for a
cover charge.
Numbers in the text, e.g. 1.2, refer to the illustra-
tions, i.e. Fig. 1, pos. 2 in this example.
Attention:
This manual is an integral part of the product
and must be read carefully before switching
on and using the microscope! It contains
important instructions and information for
safe operation and maintenance of the
product and must therefore be kept in a safe
place!
Special safety information is marked at the
edge by the lefthand symbol and highlighted
by a grey background.
Warning of hot surface.
Attention! This symbol means that incorrect
operation can damage the microscope or its
accessories.
Explanatory note.
Item is not included in all variants of the micro-
scope.
The Leica DMR microscope series consists of
several basic stands and a range of modular
components allowing an almost unlimited
variety of individual outfits.
Therefore this manual has been given a modular
layout as well to show you other possible
configurations besides your own.
The manual is divided into two main chapters:
Assembly (including a brief description of each
component) and
Operation.
Any alterations or additional information are
described on extra pages. There is a
supplementary manual for the automatic
version. The manuals are multilingual. Due to
the spiral binding you can turn the language you
want to the front. The manual can be filed in the
supplied folder with the transparent plastic
tongues.
Important notes on this manual
*
!
Text symbols and their meaning:
8
Attention:
Fire hazard! Keep lamphousings at least
10 cm (4˝) away from inflammable objects
such as curtains, wallpaper or books!
Assembly tools
You only need a few ordinary screwdrivers to
assemble your microscope. These are supplied
with the delivery. Replacements for lost tools
can be obtained from us or from a tool shop
(Fig. 1), see list of spare parts on p. 112.
Unpacking
Please compare the delivery carefully with the
packing note, delivery note or invoice. We
strongly recommend that you keep a copy of these
documents with the manual, so that you have
information on the time and scope of delivery
later when ordering more equipment or when
the microscope is serviced. Make sure that no
small parts are left in the packing material.
Some of our packing material has symbols
indicating environmental-friendly recycling.
Attention:
When taking the microscope out of its packing
and putting it onto the desk take care not to
damage the sensitive vibration-damping feet on
the bottom of the microscope.
Attention:
Do not connect the microscope and periph-
erals to the mains yet! (see page 53).
Installation site
Attention:
Make sure that the workplace is free from oil
and chemical fumes. Vibrations, direct sunlight
and major temperature deviations have a nega-
tive effect on measurements and photomicro-
graphy. This and an ergonomically designed
chair which can be adjusted in several positions
arethe basicprerequisites forfatigue-free micro-
scopy.
Assembly/General information
!
Fig. 1 Assembly tools
13 mm hexagonal
screwdriver
2Crosstip screwdriver*
3Adjustment key for
Sénarmont compensator*
4Pol centering key (long
version)*
5Centering key (short
version)*
6Allen key 2 mm (3 mm)*
* not part of all outfits
!
12345
6
9
Fig. 2 Back of microscope stand
1RS 232 C* interface, 2Connection for 12V 100 W transmitted
light lamp* , 3Connection for 12 V 100 W incident light lamp*,
4Ground connection, 5Mains connection, 6115/230 V**
switchover, 7Space for extra lamphousing or switchable
mirror, 8Fuses (T4A), 9Lamphousing 106*: screw for opening
lamp housing 106, o
ONot illustrated, on the top surface of the
back of the microscope: plug connection* for photomicro
(lamp and shutter control)
The instruments and accessories described
in the manual have been checked for safety
or possible risks. Before making any altera-
tions to the equipment or combining it with
non-Leica components in a way not de-
scribed in this manual, consult the Leica
agency for your region or the main factory in
Wetzlar! Any guarantee will be rendered in-
valid if the instrument is opened or modified
in any way by unauthorised persons or if the
instrument is used in another way than the
one described in these instructions!
+ 6
-
25 + 6
-
20
9
7
6
5
8
4
2
3
1
Setting the mains voltage
Microscopes with mechanical focusing (42.12)
are automatically adapted to the local mains
voltage in a range of 120 %/230 % V. For
microscopes with motor focus (RE and RXE
models, Fig. 44), however, the selector switch at
the back of the microscope (2.6) must be set.
Attention:
For external power units the mains voltage
should always be set according to the sepa-
rate instructions supplied.
Electric safety
To ensure that the microscope and
accessories are in a perfectly safe condition,
please note the following advice and
warnings: The mains plug must only be
inserted into a grounded outlet. If an
extension cord is used, it must be grounded
as well. Using the ground connection (2.4),
any accessories connected to the
microscope which have their own and/or a
different power supply can be given the same
ground conductor potential. Please consult
our servicing personnel if you intend to
connect units without a ground conductor.
This manual suits for next models
5
Table of contents
Other Leica Microscope manuals
