Ken A Vision Research Scope T-29041 User manual

Research Scope

Instruction Manual

T-29031 Binocular Acromat Research Scope

T-29041 Trinocular Acromat Research Scope

T-29032 Binocular Semi-Plan Research Scope

T-29042 Trinocular Semi-Plan Research Scope

T-29033 Binocular Acromat Plan Research Scope

T-29043 Trinocular Acromat Plan Research Scope

T-29034 Binocular Infinity Achromat Research Scope

T-29044 Trinocular Infinity Achromat Research Scope

T-29035 Binocular Infinity Semi-Plan Research Scope

T-29045 Trinocular Infinity Semi-Plan Research Scope

T-29036 Binocular Infinity Plan Research Scope

T-29046 Trinocular Infinity Plan Research Scope

Thank you for purchasing a Ken-A-Vision Research Microscope. The modern

Ken-A-Vision research microscope is a professional laboratory instrument

designed for modern biology and medical science classrooms, clinical

experiments in biology, pharmacology, bacteriology, microbiology and

genetics. Its modular design allows for a full range of accessories. It has a

modern, ergonomic design with many advanced features so users can operate

the instrument conveniently and safely.

T-29031 T-29041 T-29032 T-29042 T-29033 T-29043

Halogen Light

12V 20 W

D DD DDD

High Bright Köhler

Illumination

D DD DDD

Low Coaxial Fine Focus

Scale 0.002mm

D DD DDD

30° Inclined Binocular

55mm - 75mm

DDD

30° Inclined Trinocular

55mm-75mm

DDD

Eyepieces - 10X D DD DDD

D I N 4 X , 1 0 X , 4 0 X ,

100X Oil Objectives

Achromat Achomat Semi-Plan

Achomat

Semi-Plan

Achomat

Plan

Achomat

Plan

Achromat

Double layered

mechanical stage

180mm x 150 mm

D DD DDD

Gimbaled N.A. 1.25

Abbé Condenser with

built-in Iris Diaphragm

and filters for Köhler

Illumination

D DD DDD

T-29034 T-29044 T-29035 T-29045 T-29036 T-29046

Halogen Light

12V 20 W

DD D DDD

High Bright Köhler

Illumination

DD D DDD

Low Coaxial Fine Focus

Scale 0.002mm

DD D DDD

30° Inclined Binocular

55mm - 75mm

DDD

30° Inclined Trinocular

55mm-75mm

DDD

Eyepieces - 10X DD D DDD

DI N 4X , 1 0X , 40 X,

100X Oil Objectives

Achomat

Infinity

Achomat

Infinity

Semi-Plan

Infinity

Semi-Plan

Infinity

Plan

Infinity

Plan

Infinity

Double layered

mechanical stage

180mm x 150 mm

DD D DDD

Gimbaled N.A. 1.25

Abbé Condenser with

built-in Iris Diaphragm

and filters for Köhler

Illumination

DD D DDD

OPERATION

Illumination: On any light microscope, the ability to adjust light is probably

the most import element after the quality of the lens systems. The Ken-A-Vision

Research Microscope has 4 distinct mechanisms to adjust light intensity, and

thereby allowing for High Bright Köhler Illumination. The degree of light inten-

sity needed depends on individual conditions such as specimen

density, contrast, translucence and staining, objective magnification and

individual users eyesight Avoid turning the light onto full brightness as this

shortens the life of the bulb.

Too little or two much light is rarely recommended. Light entering into the

objective from the sub-stage illuminator may be modified by:

•ADimmer (Rheostat) switch on the bottom left side

•ADiscIris diaphragm (Field Diaphragm) on the top of the illuminator

in the base

•ADiscIris diaphragm (Condenser Diaphragm) mounted within the

Abbé Condenser

• Raising and lowering the condenser

The amount of light may also be manipulated by moving the gimbaled

condenser by using the three substage manipulating screws.

High Bright Köhler Illumination:

Köhler Illumination - Defined

Köhler illumination is a method to reduce an old problem of transmitted

light microscopes. The filament of illuminating bulb of the microscope is often

visible in the same plane as the sample, reducing resolution and sharp focus.

One simple, partial solution is to reduce the amount of light striking the

specimen, however reducing voltage to reduce light intensity may also reduce

the breath of wavelengths (hues) reaching the specimen. Various other

modifications have been done over time including opal bulbs, or placing a

glass diffuser lens in front of the bulb. Though all these techniques work to

some degree, they tend to reduce the quality (some wavelengths of light are

lost) or the amount of incident light. Köhler illumination can be created

on microscopes with quality diaphragms, a moveable (both horizontally and

vertically) condenser, and a variable light source. Uniformity of light is

essential to avoid shadows, glare, and inadequate contrast when taking

photomicrographs. Köhler illumination overcomes these limitations.

How to set up your Ken-A-Vision Research Microscope

for High Bright Köhler Illumination

• Focus on the specimen with whatever objective desired for use.

•Closethefield diaphragm (located on top of the illuminator in the

base) to its most closed position - you may see the edges of the

diaphragm within the microscope field (they may be blurry).

• Using the black condenser knob at the sub-stage back left of the

microscope, bring the edges of the field diaphragm into the best focus

possible.

• Now using the condenser centering screws of the gimbaled mount, center

the image of the closed field diaphragm in the field of view. IMPORTANT

NOTE - There are three screws in the gimbals, the rear-most one on the

right side is a locking screw, not a positioning screw. Before making ANY

adjustments, loosen this screw and then center the image as described

above using the other two screws, one on the right and one on the left.

• Lock the gimbals into position with the third screw.

•Openthefield diaphragm just enough so that its edges are just

beyond (outside) the field of view.

• Now work with the condenser diaphragm by adjusting it to introduce

the desired amount of contrast into the sample. Be careful, too much

contrast can introduce artifacts into your view, and misrepresent what you

actually see in the specimen.

• Finally adjust the light intensity to your comfort level. If available, it is bet-

ter to use a neutral density filter in the filter holder (below the bottom

of the condenser) then severely reducing the light intensity. A neutral

density filter tends to block all wave lengths equally. Reduction of volt-

age to the light source, particularly with Tungsten lights, tends to alter the

actual wave spectrum being emitted by the light source and thereby the

hues of light actually being transmitted through the specimen to the

objectives.

Focus:

Place a specimen slide on the center of stage. Before looking into the

eyepieces and using either the 4X or 10X objectives with the 10X eyepiece,

raise the stage as high as possible.

While looking through the eyepieces, slowly rotate the coarse focus knob

(stage will lower). When image begins to appear, switch to using the fine focus

knob to obtain a sharp image. To switch to higher powers, rotate the

objectives clockwise (from right to left). When changing to other objectives,

the T-290XX objective lens is parfocal, and only a slight adjustment (if any) of

the fine adjust, will bring the image quickly into clear focus.

As you go up in power, the objectives are getting closer to the cover slip of the

specimen (the working distance is getting smaller). The 40X and above

objectives are all spring-loaded so they should slide onto the cover slip

WITHOUT any force. If you have to force the objecting into position, STOP,

your specimen and/or cover slip may be too thick, and you risk scratching the

objective and/or smashing the slide.

The tightness of the coarse focus knob has been adjusted at the factory. If it is

too loose (the stage falls automatically), please adjust the tension using the

coarse adjustment tension ring (located on the right side of the microscope,

between the coarse adjust knob and the body of the microscope. To tighten

the tension, turn the knob clockwise (towards the back), to loosen the tension

turn the knob counterclockwise (towards the front of the microscope) until a

suitable position is found. The stage should not move on its own as it sits

waiting for your next use.

Bulb replacement - (Replacement bulb is Ken-A-Vision

Part # T6V20WP)

IMPORTANT NOTE: Before replacing the lamp, turn OFF the power switch

and unplug from electrical source. Place microscope on its side or back,

exposing the bottom of the unit. Unscrew the knurled knob that is towards the

front of the unit to open the access door. (Be careful, if unit has been recently

used, knurled knob could be very hot). Pull lamp door down and remove

lamp by pulling straight out of socket. When replacing the lamp, do not touch

the glass of the bulb with bare hands, as the oil from your fingers can damage

the bulb and definitely will shorten the life of the bulb. Wear gloves or keep

the protective cover on the bulb during installation. Should you accidentally

put any fingerprints on the bulb, wipe then off carefully using a clean cloth.

Useful microscopy definitions and Research Scope

options

Objective Lenses

There are typically three types of objective lenses found on brightfield

microscopes - Achromat, semi-plan, and plan objectives. To

understand the difference, realize that all microscope lenses are curved, which

results in an image that is slightly out of focus at the edges, and may produce

chromatic or spherical aberrations due to this curved shape.

Achromat Objective - An achromatic lens (achromat) is an objective

lens that is designed to limit the effects of chromatic and spherical

aberration (See notes below). Achromatic lenses are corrected by bringing

two wavelengths (typically red and blue) into focus in the same plane. An

objective that is Achromatic carries the implication (guarantee) that at least

60% of the field of view will have a quality flat focus.

Semi-Plan Objective - Semi-Plan objectives are guaranteed to have a

quality flat field of focus for 80% of the field of view. A Semi-Plan

Achromat lens is also corrected for chromatic aberrations, as described

above.

Plan Objective - Plan Objectives guarantee a quality flat field of focus for

100% of the field of view. A Plan Achromat lens will also have the same

corrections for chromatic aberrations as noted above. In Plan Objectives

when imaging the field, parts of the specimen at the edge of the visual field

are almost as well focused as those in the center. Straight lines on the

specimen appear straight throughout the visual field, without any aberrations.

Such microscopes are especially good for photomicrography. In general, the

better the plan field objective, the more expensive the lens.

Flat Field Objectives - As one looks at the field of view, there may be slight

blurring at the edge of the field. This edge blurring of Flat Field objectives is

slight and easily corrected by a small tweak of the fine focus knob as one looks

toward the edge of the field, or by moving the specimen so that the portion

that is at the edge is moved into the center of the viewing field. Flat Field

Objectives tend to be inexpensive when compared to Plan Field.

Plan Field versus Flat Field Objectives - Most educational, teaching

microscopes are Flat Field Objectives due to this cost difference.

Infinity - Infinity lenses - In microscopy, the term "infinity" means that the

objective lenses are designed to project their images to infinity, not to some

finite distance. Traditional brightlight microscopes had a "fixed" focal point at

160 mm established by standard in the 1800's. The infinity optical system

provides an "infinite" region of parallel light between objective and eyepiece,

pushing the image out approaching infinity

Aberrations - Chromatic, Achromatic or Spherical - Any change from

idealized and/or uniform light conditions coming through a

lens is called an aberration.

Chromatic aberration - White light is made up with all

the colors or wavelengths of the visible spectrum. These color

components travel through the lens at different wave lengths

and in doing so the lenses of the microscope may cause the

different colors to refract (bend from a straight line) at

different angles; This is called chromatic aberration.

Achromatic aberrations - Achromatic aberrations are

caused by distortions in the smoothness of the lenses. The

image appears blurry in places.

Spherical aberrations - Spherical aberration occurs

when the rays of light passing through the center of the lens

tend to focus on a different plane than those entering near

the edge of the lens. This is because the curvature of the

lens is not perfect. The variance in cost of microscopes, in

general, is often attributed to the cost of lenses needed to

cure this problem. Often the only way that these problems

can be overcome is by using a series of lenses, each lens

carefully being designed to counteract the aberrations of the

other lenses.

Phase Contrast microscopy - Phase contrast microscopy is an

illumination technique useful when looking at transparent specimens, which

because they lack color are difficult to visualize. This modification of the

microscope causes small phase shifts in the light passing through a transpar-

ent specimen and these shifts are converted into amplitude or contrast

changes in the wave lengths of the light being transmitted through the image

and up to the observer's eye.

CARE AND MAINTENANCE

Cleaning

The T-290XX Research Microscopes are precision instruments. Routine or

protective care and maintenance is highly recommended.

• Always wipe off any (immersion) oil from objectives, stage and condenser

immediately. More persistent dirt, such as fingerprints, grease and/or oil,

may be removed with soft cotton or lens tissue, lightly moistened with lens

cleaner.

• Frequently wipe off eyepieces, objective lenses and condenser lens with

high quality lens paper. Particularly remove any oils accumulated from

finger prints and hands resting on microscope parts.

• Dry objective lenses, condenser lens, and stage immediately after any

water spillage.

• Never leave the microscope with any of the objectives or eyepieces

removed as dust and debris will collect in tubes and on ends of eyepieces.

• Always protect the microscope with the dust cover when not in use.

• Store microscope in a dry environment, particularly avoid storing unit

where it may be exposed to any chemical volatiles or other airborne

corrosives.

Long Term Maintenance

All optical and mechanical equipment requires periodic servicing to keep it

performing properly. Ken-A-Vision recommends the user anticipate this need

by establishing a schedule of regular preventive maintenance to help to assure

long life and sustain optimum performance of your instrument. Such a

program of planned preventive maintenance should be done by qualified

trained personnel. This maintenance should include:

• A thorough cleaning of eyepieces and objective lenses

• Cleaning and checking for corrosion of retractable lenses

• Adjustment of all mechanical parts including the coarse and fine adjust,

tension adjustment, mechanical stage, 360° head rotation

• Electrical system -power cord (damage, fraying, other wear), check for

evidence of any wire or socket damage within the light bulb compartment

• Check for any rust or corrosion, other physical damage to the unit.

Ken-A-Vision has quality technicians on staff to repair and service your

microscopes.

Contact Ken-A-Vision at 1.816.353.4787 for more details.

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