Fisherbrand 12867913 User manual

Manual de funcionamiento

Dispensador de botella

Horizontal

Electrophoresis

Units

Instruction Manual - Gebrauchsanweis-

ung - Manuel d’utilisation - Manual de

funcionamiento

Bottle Top Dispenser - Flaschenaufsatz-Dispenser - Distributeur pour

ﬂacons - Dispensador de botella

Bottle Top

Dispenser

12867913

2.5 mL

12877913

5.0 mL

12887913

10 mL

12897913

30 mL

12807923

50 mL

12817923

100 mL

Flaschenaufsatz-

Dispenser

Distributeur pour

ﬂacons

Dispensador

de botella

www.eu.ﬁshersci.com/ﬁsherbrand

Bottle Top Dispenser 4

• Intended use of the instrument 5

• Speciﬁcations 5

• General safety instruction 5

• Diagram of the Bottle Top Dispenser 5

• Restriction of use 5

• Operating instructions 5

• Autoclaving 5

• Troubleshooting Guide 5

• List of recommended reagents 5

• Chemical Resistance Chart at 20°C 5

Intended use of the instrument 6

Speciﬁcations 6

General safety instruction 6

• A) Dispenser when not in use : 6

• B) Dispenser when in use : 6

Diagram of the Bottle Top Dispenser 7

Restriction of use 8

• Before using the Dispenser 8

• Assembly 8

Operating instructions 8

• Priming 8

• Dispensing 8

• Volume adjustment knob 9

• User calibration procedure 9

• Maintenance / Cleaning 9

• Dis-assembling the Dispenser for

Cleaning and Servicing 10

Autoclaving 13

• Dis-assembling for Autoclaving 13

• Re-assembling after autoclaving 13

Troubleshooting Guide 14

List of recommended reagents 15

Chemical Resistance Chart at 20°C 16

Flaschenaufsatz-Dispenser 20

• VERWENDUNGSZWECK 21

• SPEZIFIKATIONEN 21

• Diagram of the Bottle Top Dispenser 21

• Einsatzgrenzen 21

• Gebrauchsanleitung 21

• AUTOKLAVIEREN 21

• Fehlerbehebung 21

• List of recommended reagents 21

• Chemische Beständigkeit bei 20 °C 21

VERWENDUNGSZWECK 22

SPEZIFIKATIONEN 22

Allgemeine Sicherheitshinweise 22

• A) Wenn der Dispenser nicht in Gebrauch ist: 22

• B) Wenn der Dispenser in Gebrauch ist: 22

Diagram of the Bottle Top Dispenser 23

Einsatzgrenzen 24

• Vor der ersten Inbetriebnahme 24

• Montage 24

Gebrauchsanleitung 24

• Vorpumpen: 24

• Volumenregler 25

• Kalibrierung 25

• Wartung/Reinigung 25

• Demontage des Dispensers zur

Reinigung und Wartung: 26

AUTOKLAVIEREN 27

• Demontage für das Autoklavieren 27

• Montage nach dem Autoklavieren 28

Fehlerbehebung 29

List of recommended reagents 30

Chemische Beständigkeit bei 20 °C 31

Table of content

www.eu.ﬁshersci.com/ﬁsherbrand

Distributeur pour ﬂacons 35

• Finalité d’utilisation de l’instrument 36

• Spéciﬁcations 36

• Instructions générales de sécurité 36

• Diagram of the Bottle Top Dispenser 36

• Restrictions d’utilisation 36

• Instructions d’utilisation 36

• Autoclavage 36

• Dépannage 36

• Liste des réactifs recommandés 36

• Tabla de resistencia química a 20 °C 36

Finalité d’utilisation de l’instrument 37

Spéciﬁcations 37

Instructions générales de sécurité 37

• A) Lorsque le distributeur n’est pas utilisé : 37

• B) Lorsque le distributeur est utilisé : 37

Diagram of the Bottle Top Dispenser 38

Restrictions d’utilisation 39

• Avant d’utiliser le distributeur 39

• Assemblage 39

Instructions d’utilisation 39

• Amorçage 39

• Distribution 39

• Molette de réglage du volume 40

• Procédure d’étalonnage par l’utilisateur 40

• Maintenance / Nettoyage 40

• Démontage du distributeur pour le nettoyage et la

maintenance 41

Autoclavage 44

• Démontage pour l’autoclavage 44

• Remontage après autoclavage 44

Dépannage 45

Liste des réactifs recommandés 46

Tableau de résistance chimique à 20 °C 47

Dispensador de botella 51

• Uso previsto del instrumento 52

• Especiﬁcaciones 52

• Instrucciones de seguridad generales 52

• Diagram of the Bottle Top Dispenser 52

• Limitaciones de uso 52

• Instrucciones de funcionamiento 52

• Esterilización por autoclave 52

• Solución de problemas 52

• Lista de reactivos recomendados 52

• Tabla de resistencia química a 20 °C 52

Uso previsto del instrumento 53

Especiﬁcaciones 53

Instrucciones de seguridad generales 53

• A) Dispensador, cuando no se está utilizando : 53

• B) Dispensador, cuando se está utilizando : 53

Diagram of the Bottle Top Dispenser 54

Limitaciones de uso 55

• Antes de usar el dispensador 55

• Montaje 55

Instrucciones de funcionamiento 55

• Cebado 55

• Dispensación 55

• Mando de ajuste del volumen 56

• Procedimiento de calibración por parte del usuario 56

• Mantenimiento / Limpieza 56

• Desmontaje del dispensador para trabajos

de limpieza y mantenimiento 57

Esterilización por autoclave 60

• Desmontaje para realizar la esterilización

por autoclave 60

• Montaje después de la esterilización por autoclave 60

Solución de problemas 61

Lista de reactivos recomendados 62

Tabla de resistencia química a 20 °C 63

Table of content

Horizontal

Electrophoresis

Units

Bottle Top Dispenser

Instruction Manual

Bottle Top

Dispenser

12867913

2.5 mL

12877913

5.0 mL

12887913

10 mL

12897913

30 mL

12807923

50 mL

12817923

100 mL

www.eu.ﬁshersci.com/ﬁsherbrand

Bottle Top Dispenser

Instruction Manual

www.eu.ﬁshersci.com/ﬁsherbrand

Intended use of the instrument

The Bottle Top Dispenser is a general purpose laboratory instrument intended for use in laboratories for dispensing reagents and

chemicals which are compatible (Please refer the compatability chart on page XX) with the instruments.

Speciﬁcations

Vol.range mL Increment mL Accuracy

±% ±mL

0.25-2.5 0.05 0.6 0.015 0.2 0.005

0.5-5 0.1 0.5 0.025 0.1 0.005

1-10 0.2 0.5 0.050 0.1 0.010

2.5-30 0.5 0.5 0.150 0.1 0.030

5-60 1.0 0.5 0.300 0.1 0.060

10-100 2.0 0.5 0.500 0.1 0.100

General safety instruction

A) Dispenser when not in use :

• Piston Barrel should always be empty

• Nozzle cap should be ﬁxed on the nozzle

B) Dispenser when in use :

• Nozzle cap should be removed

• Place a receiving vessel under nozzle before starting the operation

• Never use force

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Diagram of the Bottle Top Dispenser

Bottle Top Dispenser

Instruction Manual

www.eu.ﬁshersci.com/ﬁsherbrand

Restriction of use

NEVER use the Dispenser with :

• Liquids which are not compatible with PTFE, FEP and Borosilate Glass

• Hydroﬂuoric acid

• Liquids which contain solid particles

• Temperature limits are 15°C to 40°C

Before using the Dispenser

Check that the instrument has not been damaged in transit

Assembly

The Dispenser is packed with the dispenze nozzle attached

and the inlet feed tube removed. The lenght of FEP inlet tubing

provided should be adjusted to ﬁt the particular reservoir. Longer

lengths on inlet tube are available on request. The threaded

platform base of the Dispenser has a 30mm screw thread. The

assembled Dispenser is screwed to the reservoir using gentle

had torque applied to the threaded platform base only. Removal

should also be by means of hand torque applied to this same

base. Do not operate the piston until the unit is safely and fully

mounted on the reservoir bottle. Six adaptators are supplied to

suit containers with a 28mm, 30mm, 32mm, 36mm, 40mm and

45mm screw neck.

Operating instructions

Priming

Place a container under the Dispenser’s delivery nozzle. Remove the Nozzle Cap. Set the “Volume adjustment knob” to the maximum

volume to enable free movement of the piston.

Prime the unit with a few gentle up and down strokes, taking the piston right down to it’s lowest stop position and lifting it up.

Repeat until a steady bubble free ﬂow is visible in the barrel.

Dispensing

• Ensure that the nozzle cap is removed.

• Ensure that a receiving container is placed the nozzle.

• Dispenser is now ready for dispensing.

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Volume adjustment knob

Volume adjustment knob is simple and

easy to use. It is screw type knob. Simply unscrew the knob and slide up or down to

set the desired volume by aligning the pointer on the volume adjustment knob with

the scale on the main housing.

Tighten the screw after aligning with the scale.

User calibration procedure

Dispenser has been laboratory calibrated to its nominal volume. However, due to changes in

environmental conditions and the viscosity of the media which you dispense, a re-calibration might be

required. You can either re-calibrate at regular intervals such as once a week or whenever you notice

that the dispensed volume is different from the volume displayed by the unit.

To fully re-calibrate your Dispenser follow the following steps:

1. Set the Dispenser to the nominal volume or any other volume which is the most common volume you

dispense.

2. Follow the common rules for calibration used in statistical quality control (ISO 8655/2). Set the volume

and Dispense ﬁve full volumes of distilled water at 20°C on Electronic Balance to establish the actual

mean volume of liquid dispensed. If the gravitational average result varies from the volume displayed,

you should re-calibrate the Dispenser.

3. For re-calibration pull the cap outwards to expose the Calibration Nut.

4. Using the calibration tool, turn the calibration nut clockwise to reduce the volume and anticlockwise

to increase the volume. Repeat this procedure a few times till the desired volume is achieved.

Maintenance / Cleaning

Note: All maintenance should be carried out wearing suitable eye protection and protective clothing. If

in doubt, consult your safety ofﬁcer.

1. Make sure that the Dispenser is completely empty.

2. Place the instrument into an empty sink together with its reservoir.

3. Unscrew the threaded platform base from the reservoir and lift the dispenser’s intake tube carefully

out of the reservoir, whilst tapping it against the reservoir’s aperture to shake off any droplets from

Volume adjustment knob

Cap

Bottle Top Dispenser

Instruction Manual

www.eu.ﬁshersci.com/ﬁsherbrand

the intake tube.

4. Hold the dispense nozzle over the aperture of the reservoir and apply gentle piston strokes in order to return any syringe contents

into the reservoir.

5. Empty the instrument completely and ﬂush thoroughly with distilled water.

6. If the piston barrel is still not completely clean, you need to dis-assemble the dispenser. Refer Dis-assembling procedure given

below.

Dis-assembling the Dispenser for Cleaning and Servicing

Procedure to dis-assemble the PISTON

• 1- Pull the cap outwards to expose the Calibration Nut.

• 2- Unscrew the Calibration Nut with the help of calibration tool to dis-assemble the Piston and shaft out of the main housing.

• 3- Unscrew the piston from the shaft

Procedure to dis-assemble the BARREL

• 1- Unscrew the Barrel Cap using the special tool provided with the instrument.

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