Biotage PRESSURE+ 96 User manual

413248-A

BIOTAGE SWEDEN AB US TECHNICAL SUPPORT EU AND GLOBAL TECHNICAL SUPPORT

Box 8, SE-751 03 Uppsala, Sweden Phone: +1 800 446 4752 press 3 Phone: +46 (0)18 56 59 11

Visiting Address: Vimpelgatan 5 US-1-pointsu[email protected] EU-1-p[email protected]om

Switchboard: +46 (0)18 56 59 00

[email protected], www.biotage.com

Biotage PRESSURE+96

Positive Pressure Manifold

User Manual

PRESSURE+ 96 User Manual Page 2 of 15

Contents

Safety....................................................................................................3

Intended Use ........................................................................................3

Warranty ..............................................................................................3

Service.................................................................................................3

Equipment Safety Standards ...................................................................3

Labels ..................................................................................................3

Safety Considerations.............................................................................4

Installation............................................................................................5

Site Requirements..................................................................................5

Gas Source ........................................................................................5

Unpack and Install .................................................................................5

Packing List........................................................................................7

System Description................................................................................8

Load the Plates......................................................................................8

Flow Control..........................................................................................9

Operation ............................................................................................ 10

Step-by-Step Instructions ..................................................................... 10

Maintenance ........................................................................................ 13

Replace the Column Seal....................................................................... 13

Clean ................................................................................................. 13

General Information ............................................................................ 14

Trademarks.........................................................................................14

Manufacturer....................................................................................... 14

Sales Offices and Distributors .............................................................14

Technical Support ............................................................................. 15

PRESSURE+ 96 User Manual

Page

Safety

Intended Use

Biotage PRESSURE+ 96 is intended for laboratory use only and has to be

operated by trained professionals. All operations must be performed:

According to the user documentation delivered with the equipment.

According to instructions available at www.biotage.com.

According to instructions given by the technical support staff from

Biotage.

Within limits set by the technical specification.

Failure to follow these instructions and operate within the limits set by the

technical specification may result in personal injury and/or equipment damage.

Warranty

PRESSURE+ 96 is warranted for 90 days against defects in materials and

workmanship under normal operating conditions and proper maintenance (see

page 13).

Service

All service or adjustments shall be performed by an authorized Biotage service

engineer. Before handing over the unit for service, it should be cleaned from

harmful residues as described in the Clean section on page 13.

Equipment Safety Standards

PRESSURE+ 96 and its instruction materials are certified by Biotage to comply

with:

CE-Marketing Directive 93/68/EEC

Machinery Directive 2006/42/EC

Labels

Labels used on PRESSURE+ 96:

In accordance with all the essential requirements of all applicable

European product directives; see “Equipment Safety Standards”

above.

Consult accompanying user documentation.

Manufacturer.

PRESSURE+ 96 User Manual Page 4 of 15

Safety Considerations

Many reagents and samples used with PRESSURE+ 96 can pose chemical,

biological and radiological hazards. You must always understand the potentially

hazardous effects of all the materials you work with. To prevent personal injury

and/or equipment damage, we recommend that you always use impermeable

gloves, chemical safety goggles and protective clothing, follow GLP (Good

Laboratory Practice), and consult your company’s safety expert for guidance.

PRESSURE+ 96 units are manufactured from aluminum, stainless steel and

plastics. Always clean up spills or overflow immediately.

PRESSURE+ 96 User Manual Page 5 of 15

Installation

Site Requirements

The PRESSURE+ 96 unit should be placed in a laboratory and operated where

there is proper ventilation or from inside a chemical fume hood when volatile

solvents are used. The PRESSURE+ 96 unit requires a compressed gas supply,

and should be located with this in mind.

Table/bench: Flat, stable, and able to support the weight of the

instrument (11.8 kg).

The dimensions of the unit is 271 x 305 x 325 mm (W x D

x H). The free space above and behind the bench should

be at least 10 cm for ventilation.

Operating

temperature: 5°C to 40°C, indoor use only

Humidity: Maximum relative humidity of 80% for temperatures up to

31°C, decreasing linearly to 50% relative humidity at 40°

Altitude: Up to 2000 meters

Gas Source

PRESSURE+ 96 operates using a compressed gas both to seal the columns and

displace liquid. Whether compressed air or nitrogen is used, or cylinders of

purified air or nitrogen, the gas should be free of moisture, particulates, and

hydrocarbons. This is essential to prevent sample contamination and general

fouling of the manifold.

There are a variety of filters available for the removal of moisture, particulates,

and hydrocarbons most commonly found within house gas supply systems. The

optimum gas supply (80 psi) is achieved by a regulator positioned between the

gas source and the unit. The supplied gas supply installation kit provides the

parts necessary to make the connection between the gas regulator and the unit.

Minimum pressure: 60 psi (4.1 bar)

Maximum pressure: 100 psi (6.9 bar)

Optimum pressure: 80 psi (5.5 bar)

Unpack and Install

WARNING

The total weight of the package including the unit is 16 kg . Follow

regional safety practices when handling, moving, and unpacking shipping

boxes and containers.

PRESSURE+ 96 User Manual Page 6 of 15

Keep hands, loose clothing, and long hair away from the manifold when

connecting the gas supply. Slight movement may occur as the unit

pressurizes.

NOTE: Biotage recommend that the boxes and packing materials are retained by

the customer in case the unit needs to be transported or stored.

PRESSURE+ 96 User Manual

Page

The unit will already have the tubing attached in the back as shown in the left

image below.

1. Open the inner box and remove the smaller boxes. These contain the gas

supply installation kit, column seal, etc; check the contents against the

packing list below.

2. Gently pull the unit from the box and set the unit on a bench or in a fume

hood. See site requirements on page 5.

3. Using the supplied gas supply installation kit, plumb the back of the unit

to the gas supply (see images below). Refer to the Gas Source section on

page 5 for gas requirements.

4. Position the unit so that the handle and gauges are facing forward and the

gas supply is in the rear. Note that the unit weighs 11.8 kg.

Connect the gas line to the right port at the back of the unit.

Packing List

PRESSURE+ 96 is shipped with the following items:

Quantity Description Part Number

1 1 mL x 96 collection plate 121-5202

1 2 mL x 96 collection plate 121-5203

1 10 mL x 24 collection plate (waste plate) PPM-A96-1024

1 96-column seal, extra

(contains a hex key and 4 positioning

screws)

PPM-A96-GSKT

1 Gas supply installation kit

(6' of 1/8" i.d. polyethylene tubing and

1/8" and 1/4" NPT connectors)

PPM-GA

1 User Manual CD PPM-Man

PRESSURE+ 96 User Manual Page 8 of 15

System Description

PRESSURE+ 96 is a positive pressure manifold designed to process 96-well

extraction plates using a pressurized gas source.

Load the Plates

PRESSURE+ 96 is compatible with all Biotage 96-well plate designs, including

fixed-well plates and modular arrays. The extraction plates should sit snugly on

top of the collection plate. Note that collection plates should allow for venting of

the positive pressure. Standard 1 mL and 2 mL collection plates from Biotage

will allow venting when paired with any of the 96-well formats from Biotage.

IMPORTANT: Other manufacturer’s collection plates may be used, however,

users must ensure that the assembly of extraction plate and collection plate can

vent when positive pressure is applied.

The handgrip on the front of the platform helps you to slide the platform with the

extraction and collection plates forward and backward under the manifold. Stack

the extraction plate onto the collection plate and set the stack onto the sliding

platform.

To lower the manifold, push the platform to the back of the track until it reaches

the stop point (under the upper manifold), then press down on the two rocker

switches on the sides of the unit simultaneously and hold for approximately 3

seconds. This will activate the upper manifold and it will self-adjust to any plate

assembly height by lowering and compressing. If you let go too soon, the upper

manifold will rise up and you can simply repeat by pressing down on the rocker

switches and holding them again for approximately 3 seconds.

To release the plates, press up on both rocker switches, and then slide the plate

assembly forward.

NOTE: The upper manifold is powered by the gas source and the speed is pre-

set and not adjustable.

IMPORTANT: An important safety feature of the unit is the requirement to hold

both rocker switches for approximately 3 seconds simultaneously to lower the

manifold. This is to keep hands clear of moving parts during compression.

Lower the manifold by pressing down on the two rocker switches simultaneously

for approximately 3 seconds.

PRESSURE+ 96 User Manual Page 9 of 15

Flow Control

The PRESSURE+ 96 unit has a two-tier gas delivery system. The flow control

switch (B) enables setting and operation of gas flow in addition to turning gas

flow on and off:

Adjustable Flow Rate (0 to 15 psi, 0 to 2.5 scfh)

Recommended for sample loading and elution. By turning the flow control switch

(B) to the left to the Adjustable Flow position, gas is channeled through the

Adjustable Flow regulator (C) and then through a rotometer (A) controlled by the

needle valve positioned at its base. This path through the rotometer provides

very precise and slow flow through the columns by limiting gas flow from 0 to 2.5

scfh (standard cubic feet per hour).

IMPORTANT: As indicated on the label, never turn the rotometer completely off.

Seating the needle valve into its orifice can damage the unit.

Maximum Flow Rate (0 to 100 psi)

Recommended for column washing, drying, etc. By turning the flow control switch

(B) to the right to the Maximum Flow position, gas is introduced at the pressure

set on the Maximum Flow regulator. The pressure range for this regulator is 0 to

100 psi and should be used when processing very viscous or dirty samples.

Turn Off the Gas

By turning the flow control switch (B) to the OFF position, no gas will flow

through the manifold.

IMPORTANT: The unit should always be in the OFF position during compression

and decompression cycles and when not in use. Always shut off the external gas

supply when the unit is not in use.

NOTE: In case of troubleshooting, for each well, with no column in place, the

flow at 25 psi should be 0.75 ±0.05 scfh (standard cubic feet per hour) of flow

through each well.

PRESSURE+ 96 User Manual Page 10 of 15

Operation

WARNING

Keep hands, loose clothing, and long hair away from the manifold when it is

moving.

To avoid spillage, ensure to stack the plates properly on the platform.

To prevent personal injury and/or equipment damage, we recommend that you

always use impermeable gloves, chemical safety goggles and protective clothing,

follow GLP (Good Laboratory Practice), and consult your company’s safety expert

for guidance.

Step-by-Step Instructions

1. Ensure the unit is connected to a gas supply; see Installation section on page 5.

2. Place a 96-well collection plate on the platform.

3. Place a 96-well extraction plate on top of the collection plate.

4. Load each well with conditioning solvent.

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