Biohit PROLINE PLUS User manual

Proline®Plus

Instruction Manual

Bedienungsanleitung

Mode d'emploi

Manual Usuario

Instruzioni d'impiego

Инструкция пользователя

5, 10, 20,

25, 50, 100

Spare

Parts

1Tip ejector

collar

2Tip cone

holder

3Tip cone

complete

4Piston

5Calibration

nut cover

6Cap

(5 pcs, blue)

Ersatzteile

Spitzen

abwurf

Konus-

halter

Spitzen-

konus

Kolben

Abdeckung

kalibrier-

schraube

Abdeckungen

(5 Stück, blau)

Piéces

détachées

Tube

d'ejection

Bague de

rétenue

Porte-cône

complet

Piston

Dessus

d'ecrou

Bouchons

(5 pièces, bleu)

Repuestos

Expulsor de

puntas

Anillo soporte

de puntas

Soporte

de puntas

completo

Pistón

Tapa del

mecanismo

de calibración

Tapas

(5 pcs, azul)

Parti di

ricambio

Collare dell'

espulsore

puntale

Contenitore

cono

Cono

ancoraggio

puntali

completo

Pistone

Copri dado di

calibrazione

Cappuccio

(5 pz, blu)

3 5, 10 20 25,50,100 200 250,500,1000 2ml, 5ml 10 ml

1726108 726108 726480 726480 726480 726481 726111 726482

2726041 726041 726041 726041 726041 726042 N/A N/A

3276044 731084 726083 726042 726039 726040 726038 726047

4726032 731076 726084 726034 726035 726036 726037 726031

5726006 726006 726006 726006 726006 726006 726006 726006

6726469 726469 726469 726469 726469 726469 726469 726469

200, 250,

500, 1000

2000,

5000,

10 000

Запасная

част

Сбрасыватель

наконечника

Держатель

посадочного

конуса

Посадочный

конус

Поршень

Крышка

калибровочного

винта

Колпачок

(5 кусок синий)

8-channel: 10, 100, 300

12-channel: 10, 100, 300

Spare

Parts

1Tip cone

housing

2Tip ejector

bar

3Tip cone

complete

4Piston

5Piston

assembly

6Calibration

nut cover

7Cap

(5 pcs, blue)

Ersatzteile

Spitzenkonus-

gehäuse

Spitzen-

abwurfs-

balken

Spitzen-

konus

Kolben

Kolben-

zusammen-

setzung

Abdeckungen

Kalibrier-

schraube

Abdeckungen

(5 Stück, blau)

Piéces

détachées

Module

porte-cônes

Barre

d'éjection

Porte-cône

complet

Piston

assemblé

Dessus

d'écrou

Bouchons

(5 pièces, bleu)

Repuestos

Carcasa del

Soporte de

puntas

Expulsor

de puntas

Soporte de

puntas

completo

Pistón

Conjunto de

pistón

Tapa del

mecanismo

de calibración

Tapas

(5 pcs, azul)

Parti di

ricambio

Alloggiamento

del cono di

ancoraggio

del puntale

Barra di

espulsione

del puntale

Cono ancor-

aggio puntali

completo

Pistone

Assemblaggio

del pistone

Copri dado di

calibrazione

Cappuccio

(5 pz, blu)

Запасная

част

Корпус

поршневой

части

Сбрасыватель

наконечника

Посадочный

конус

Поршень

системы в

сборе

Крышка

калибровоч-

ного винта

Колпачок

(5 кусок синий)

10/8 100/8 300/8 10/12 100/12 300/12

1726125 726126 726127 726128 726129 726130

2726137 726138 726138 726139 726140 726140

3726149 726150 726151 726149 726150 726151

4726146 726147 726148 726146 726147 726148

5726152 726153 726154 726152 726153 726154

6726006 726006 726006 726006 726006 726006

7726469 726469 726469 726469 726469 726469

Instruction Manual........................................1

Bedienungsanleitung..................................15

Mode d'emploi ...........................................29

Manual Usuario ..........................................43

Instruzioni d'impiego..................................57

Инструкция пользователя ..............................71

Specifications.............................................85

Proline®Plus

CONTENTS

1. INTENDED USE ........................................................... 2

2. PRODUCT DESCRIPTION.......................................... 2

3. BIOHIT OPTIFIT TIPS.................................................. 3

4. UNPACKING ................................................................ 3

5. PIPETTE HOLDER AND CAROUSEL STAND........... 4

6. CALIBRATION TOOL (ALSO TUBE OPENER).......... 4

7. OPERATING THE PIPETTE ........................................ 5

7.1. Setting the volume.............................................. 5

7.2. Sealing and ejecting tips.................................... 5

7.3. Protective tip cone ﬁlters.................................... 5

8. PIPETTING TECHNIQUES.......................................... 6

8.1. Forward pipetting (Fig. 6) ................................... 6

8.2. Reverse pipetting (Fig. 7) ................................... 6

8.3. Repetitive reverse technique.............................. 7

9. RECOMMENDATIONS FOR GOOD PIPETTING ...... 7

10. MAINTENANCE ........................................................... 8

10.1. Daily cleaning of the outer surface

of the pipette....................................................... 8

10.2. Cleaning and decontaminating the lower

part of the pipette............................................... 8

10.3. Sterilization of the pipette .................................. 9

11. TESTING THE PERFORMANCE AND

RECALIBRATION....................................................... 10

11.1. Testing the performance .................................. 10

11.2. Recalibration..................................................... 12

12. TROUBLE SHOOTING .............................................. 13

13. WARRANTY INFORMATION .................................... 14

14. PERFORMANCE SPECIFICATIONS ........................ 14

1. INTENDED USE

This liquid handling device is designed and manufactured to be

used with the IVD-product as an IVD-accessory and as a stand

alone laboratory equipment.

2. PRODUCT DESCRIPTION

Your new Biohit Proline®Plus is an autoclavable air displacement

pipette. The attractive and ergonomical design of the Proline®Plus

pipette together with its low pipetting forces reduce the risk of

repetitive strain injuries (RSI)1. The pipette is designed for both

right- and left-handed use.

The special tip cone design of the pipette offers the possibility to

use replaceable Safe-Cone ﬁlters to help prevent the contamination

and damage of the pipette. The pipette uses disposable tips.

Biohit Proline®Plus single- and multichannel pipettes

1. Note: It is knowledge that prolonged pipetting can cause RSI.

The manufacturer is not responsible for RSI or any related diseases

caused by prolonged pipetting.

Safe Cone

Colour Volume ﬁlters

Cat. No. Code Channels Range Increment Tip Standard Plus

728010 Grey 1-ch 0.1-3 μl 0.002 μl 10 N/A N/A

728020 Grey 1-ch 0.5-10 μl 0.01 μl 10 N/A N/A

728030 Yellow 1-ch 2-20 μl 0.02 μl 300 721014 N/A

728050 Yellow 1-ch 10-100 μl 0.1 μl 300, 350 721008 721018

728060 Yellow 1-ch 20-200 μl 0.2 μl 300, 350 721007 721017

728070 Blue 1-ch 100-1000 μl 1 μl 1000 721006 721016

728080 Green 1-ch 500-5000 μl 10 μl 5000 721005 721015

728090 Red 1-ch 1-10 ml 20 μl 10 000 721005 721015

728515 Grey 1-ch 5 μl 10 N/A N/A

728520 Grey 1-ch 10 μl 10 N/A N/A

728530 Yellow 1-ch 20 μl 300 721014 N/A

728535 Yellow 1-ch 25 μl 300 721008 721018

728545 Yellow 1-ch 50 μl 300 721008 721018

728550 Yellow 1-ch 100 μl 300, 350 721008 721018

728560 Yellow 1-ch 200 μl 300, 350 721007 721017

728565 Blue 1-ch 250 μl 1000 721006 721016

728567 Blue 1-ch 500 μl 1000 721006 721016

728570 Blue 1-ch 1000 μl 1000 721006 721016

728575 Green 1-ch 2000 μl 5000 721005 721015

728580 Green 1-ch 5000 μl 5000 721005 721015

728590 Red 1-ch 10 ml 10 000 721005 721015

728120 Grey 8-ch 0.5-10 μl 0.01 μl 10 N/A N/A

728130 Yellow 8-ch 10-100 μl 0.1 μl 300, 350 721008 721018

728140 Orange 8-ch 30-300 μl 0.2 μl 350 721007 721017

728220 Grey 12-ch 0.5-10 μl 0.01 μl 10 N/A N/A

728230 Yellow 12-ch 10-100 μl 0.1 μl 300, 350 721008 721018

728240 Orange 12-ch 30-300 μl 0.2 μl 350 721007 721017

3. BIOHIT OPTIFIT TIPS

The full range of Biohit pipette tips are recommended for use with

Biohit Proline®Plus pipettes. Biohit standard tips are made of virgin

polypropylene. Biohit also offers a full range of SafetySpace ﬁlter

tips. Biohit standard tips are available as bulk packages, space

saving reﬁll systems and autoclavable (121°C, 20 min, 1 atm) trays.

Pre-sterilized tips in trays are also available. (Fig. 1.)

Fig. 1.

4. UNPACKING

The Biohit Proline®Plus pipette package contains the following

items:

1. Pipette

2. Tip

3. Calibration tool (also tube opener)

4. Safe-Cone ﬁlters (pipettes >10 μl and forceps)

5. Grease

6. Instruction manual

7. Performance certiﬁcation in accordance with ISO 8655-6

Please check that all items are included and that no damage has

occurred during shipment.

5. PIPETTE HOLDER AND CAROUSEL STAND

For convenience and safety always keep the pipette vertically on

its own holder, carousel or linear stand when not in use.

Cat.No. Product

725600 Biohit Carousel Stand for 6 pipettes

725610 Biohit Pipette Holder for 1 pipette

725620 Biohit Linear Stand

6. CALIBRATION TOOL (ALSO TUBE OPENER)

The calibration tool is designed for the following purposes (Fig. 2):

1. Tool for recalibration (A).

2. Opening tool for the lid of the calibration nut (B).

3. Opening tool for the tubes (C).

Fig. 2.

7. OPERATING THE PIPETTE

7.1. Setting the volume

The volume of the pipette is clearly shown through the display.

The volume setting is carried out with the following steps (Fig. 3):

1. Set the volume by rotating the operating button (clockwise to

decrease the volume and counterclockwise to increase).

Fig. 3

–

Note: Do not rotate the operating button outside the volume range

of the pipette (Chapter. 2.)

7.2. Sealing and ejecting tips

To ensure the maximum accuracy and precision Biohit tips are

recommended to be used with Proline®Plus pipettes. Before ﬁtting

a tip ensure that the pipette tip cone is clean. Press the tip on the

tip cone of the pipette.

Eject the tip by pressing the tip ejector with your thumb (Fig. 4).

Make sure that the tip is disposed into a suitable waste container.

Fig. 4. Tip ejector

7.3. Protective tip cone ﬁlters

The tip cone design of the Biohit pipettes (>10 μl) allows the use of

Biohit Safe-Cone ﬁlters in the tip cones. These removable ﬁlters

prevent liquids and aerosols from entering the pipette and, thus,

protects the pipette from contamination and damage.

The Safe-Cone ﬁlters are available as Standard or Plus versions. It

is recommended to use the Standard ﬁlter for general applications

and the Plus ﬁlter for more demanding applications such as cell

culture, bacterial and virological work and molecular biology.

Filters need to be changed regularly. The interval for filter

changing is application dependant but the recommendation is to

change the ﬁlter daily (after 50 - 250

pipetting cycles) and always in case

of over-aspiration. (See Chapter 2

for ordering information.) The Biohit

Filter Foreceps should be used to

avoid touching the soiled filters

by hand (Fig.5). See Chapter 2 for

ordering information. Make sure that

the ﬁlter is disposed into a suitable

waste container. Clean the tip cone

if needed and put the new ﬁlter in

place.

8. PIPETTING TECHNIQUES

Two basic pipetting techniques, forward and reverse pipetting, are

associated with Proline®Plus pipettes. Forward pipetting is the

most common used pipetting technique. The technique employs

the blow-out function ensuring complete delivery of the liquid.

Reverse pipetting is recommended for highly viscous, biological

or foaming liquids, or very small volumes of liquid. A selected

volume plus an excess is aspirated into the tip. The delivery is done

without blow-out, and, thus, the excess volume remains in the tip.

The reverse technique also facilitates the repeated delivery of the

same volume. If reverse pipetting technique is used the pipette

might need recalibration.

8.1. Forward pipetting (Fig. 6)

1. Fit the tip onto the pipette tip cone.

2. Press the operating button to the ﬁrst stop.

3. Place the tip just under the surface of the liquid (2-3 mm)

and smoothly release the operating button up to the starting

position. Wait one second. Carefully withdraw the tip from the

liquid, touching against the edge of the container to remove

excess from the outside of the tip.

4. Dispense the liquid by pressing the operating button to the

ﬁrst stop. After a short delay press the operating button to the

second stop. This action will empty the tip.

Fig. 6. 0

Fig. 5.

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