BIO RAD NGC User manual

2Connect the power cable and USB to the back of the FC.

Plug in the power supply and connect the USB cable into

a USB port on the NGC Chromatography System.

Introduction

The NGC Fraction Collector provides versatile options for

preparative and analytical chromatography applications. The

high-capacity and ﬂexible rack options enable fractions ranging

in size from milliliters to liters to be collected and accessed

freely during a puriﬁcation run.

Setting Up the NGC Fraction Collector

This quick guide outlines the process of installing and plumbing

the NGC Fraction Collector to your NGC System. The fraction

collector (FC) can be installed on a laboratory bench or in a

coldroom or chromatography refrigerator.

1Open the shipping box and remove the foam packaging.

Carefully lift the FC from the box. Do not lift the FC by the

gantry; reach down and lift it from the base. Grip the base

of the FC by the sides to lift from the shipping box.

Check the box for the tubing kit, external power supply,

and USB cable. The tubing kit contains:

 92 cm green polyetheretherketone (PEEK) tubing

from NGC union to dispense head for F100 systems

(ﬁttings on both ends)

 92 cm orange PEEK tubing for F10 systems (ﬁttings

on both ends)

 92 cm clear ﬂuorinated ethylene propylene (FEP)

waste line from dispense head to union (ﬁttings on

both ends)

 150 cm clear FEP waste line from union to waste

bottle (ﬁtting on one end)

 Black magnetic union to be attached to side of NGC

System to act as anchor point for FC tubing; position

as necessary to avoid kinking any of the tubes

Installing the NGC Fraction Collector

CHROMATOGRAPHY SYSTEMS

NGC™Fraction Collector Installation

Quick Guide

Dispense head height adjustment

Standard racks

USB port Power port

Drip tray

Dispense head cover

Gantry

Plumbing the NGC Fraction Collector

Use the supplied tubing and union to plumb from the NGC System

to the dispense head on the fraction collector. Plumb the waste line

from the dispense head to the waste bottle via the union.

1Open the dispense head cover using a small

Phillips screwdriver.

2Connect the sample tube to the dispense head. Use the

cutout in the back of the cover to insert a ﬁttings tightener

and tighten.

3Attach the waste line from the dispense head waste port to

the union on the side of the NGC System.

4Route both tubes through the top of the dispense head

cover by sliding them into the rubber grommet. Finally, close

the dispense head cover and fasten with the screw.

5Attach the tubes to the union so the direction of ﬂow is as

shown below.

Sample tube

To FC

inlet port

From NGC

From FC

waste port

To waste

bottle

Dispense head

waste port

Dispense head

cover

Waste line

Rubber

grommet

Cutout

Dispense head

Completing NGC Fraction Collector Installation

After plumbing the NGC Fraction Collector, complete the

installation and adjustments.

1Place the plastic drip tray on the FC with the notched corner

facing the left rear of the FC platform.

2Place the metal rack tray into position, ensuring it is aligned

correctly on the locating pins.

3Turn on the fraction collector using the power button on

the front.

4The gantry and dispense head will move to the home

position when the NGC is powered up and the fraction

collector is detected by the system.

Note: The gantry will move quickly from the home position

to the ﬁrst collection tube at the start of a run. Ensure there

are no objects in the way before starting collection.

5Place the racks on the FC, ensuring they are correctly

aligned on the raised locating pins. Identiﬁer labels on the

racks should face right.

6Before using the fraction collector, adjust the z-height of the

dispense head to sit within 10 mm of the collection vessels.

10000076439 Ver B (12005758) US/EG 17-0738 0617 Sig 1216

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Bio-Rad

Laboratories, Inc.

Life Science

Group

PEEK is a trademark of Victrex plc. Phillips is a trademark of Phillips Screw Co.

Ordering Information

Catalog # Description

12 0 0 374 8 Peltier Rack Power Supply, 4-channel power supply for up to

4 Peltier Rack Modules

12 0 0 374 9 Peltier Rack Module, 1 insulated rack per module

12003759 Insulated Rack for 50 ml Tubes, for 14 x 50 ml tubes

12003751 Insulated Rack for 15 ml Tubes, for 30 x 15 ml tubes

12 0 0 3747 Peltier Rack Module Microtiter Plate Adaptor, for 2 deep well microtiter plates

12003752 Rack for 13 mm Tubes, for 96 x 13 mm tubes

12003755 Rack for 16 mm Tubes, for 75 x 16 mm tubes

12003756 Rack for 18 mm/15 ml Tubes, for 70 x 18 mm tubes

12003757 Rack for 50 ml Tubes, for 27 x 29 mm tubes

12003754 Rack for Deep Well Microtiter Plates, for 2 x 24-, 48-, or 96-well plates

12003753 Rack for 1.5–2 ml Capless Microtubes, for 96 tubes

12003760 Plate Rack Riser

Visit bio-rad.com/NGCFC for more information.

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