Bio-Optica AUS124 Troubleshooting guide

User and service manual AUS124 from sn 101711486

Bio-Optica Milano S.p.A. Via San Faustino 58 - 20134 Milano

Phone +39 02.21.27.13.1 - Fax Italia +39 02.21.53.000 - Fax Export +39 02.21.54.155

www.bio-optica.it – info@bio-optica.it – claims@bio-optica.it

Publication date: 09/04/2018

Rev. 04

AUTOMATIC STAINER AUS124

Code: 40-400-000

User and service manual AUS124 from sn 101711486

Bio-Optica Milano S.p.A. Via San Faustino 58 - 20134 Milano

Phone +39 02.21.27.13.1 - Fax Italia +39 02.21.53.000 - Fax Export +39 02.21.54.155

www.bio-optica.it – info@bio-optica.it – claims@bio-optica.it

Publication date: 09/04/2018

Rev. 04

USE PRECAUTIONS

Before using the instrument, read carefully the instructions and warnings contained in this

manual and keep it for further reference. They supply important indications regarding the

functions and safety for installing, using and maintaining the instrument.

Bio-Optica Milano S.p.A. cannot be held responsible for any damage caused by improper or

incorrect use and by the non-observance of any of the prescription provided in this manual

and by the safety regulations in force.

1. After unpacking, make sure that the instrument is complete and not damaged by transport.

2. Before connecting the instrument to the power supply make sure that its rating corresponds to that of the power

supply.

3. This instrument must only be used for the purpose for which it was designed, that is, as automatic stainer for laboratory

use. Any other use is to be considered improper and therefore hazardous.

4. The instrument must only be used by authorized and professionally qualified technician.

5. The electrical safety of this instrument can be guaranteed only if it is correctly connected to an efficient earth circuit

as indicated by current electrical safety regulations. It is necessary to check this fundamental safety prerequisite, and if

in doubt, ask to check the circuit. The instrument is provided with a power supply cable having 2 wires + ground tap that

have to be connected to the power supply socket.

6. Do not remove the chassis or parts of it during operation. Switch off the instrument and disconnect the power supply

cable before opening it. This operation must to be effected only by authorized and professionally qualified technician.

7. To eliminate instrument malfunctioning risks, do not work near strong magnetic fields and do not use transmitters

such as cellular phones near the instrument. In case of serious malfunctioning switch off the instrument and contact the

Technical Assistance Service.

8. All waste material, both infectious and radioactive, deriving from the appliance working cycle must be disposed in

compliance with the regulation in force.

This appliance is marked from this symbol, in compliance with EU directive 2002/96/CE regarding

electric and electronic appliances waste. This mean that the instrument, at the end of its useful life, must be collected

separately from other refuse. The user must deliver it to the special differentiated refuse collection centres, that are

predisposed by the public authority.

9. The contents of this manual is subject to change without further notice.

10. Please find enclosed the declaration of conformity.

User and service manual AUS124 from sn 101711486

Bio-Optica Milano S.p.A. Via San Faustino 58 - 20134 Milano

Phone +39 02.21.27.13.1 - Fax Italia +39 02.21.53.000 - Fax Export +39 02.21.54.155

www.bio-optica.it – info@bio-optica.it – claims@bio-optica.it

Publication date: 09/04/2018

Rev. 04

11. Graphic symbols indicated on the label (positioned near the instrument’s power supply socket):

Symbol for CATALOGUE NUMBER:

Symbol for SERIAL NUMBER:

Symbol for ALTERNATING CURRENT:

Symbol for FUSE:

Symbol for CONSULT THE INSTRUCTIONS:

Symbol for EC MARKING:

Symbol for IN VITRO DIAGNOSTIC-MEDICAL DEVICE:

Symbol for DISPOSAL OF ELECTRIC AND ELECTRONIC EQUIPMENT:

Symbol for DATE OF MANUFACTURE:

Symbol for MANUFACTURER:

User and service manual AUS124 from sn 101711486

Bio-Optica Milano S.p.A. Via San Faustino 58 - 20134 Milano

Phone +39 02.21.27.13.1 - Fax Italia +39 02.21.53.000 - Fax Export +39 02.21.54.155

www.bio-optica.it – info@bio-optica.it – claims@bio-optica.it

Publication date: 09/04/2018

Rev. 04

INDEX

1) Technical features. Page 5

2) Installation. Page 7

3) Water connections. Page 7

4) Control panel. Page 11

5) Date and hour setup. Page 12

6) Instrument’s start. Page 13

7) Instructions for the rotation of the dish holder disk. Page 17

8) Parameters setup and programming. Page 19

9) Alarms. Page 31

10) Maintenance and filters replacement.Page 33

11) Programmed maintenance and Instrument cleaning.Page 34

12) Main safety elements. Page 34

13) Main components. Page 34

14) Problem solving. Page 36

15) Fuses replacement. Page 37

16) Electric wiring diagram. Page 38

17) Declaration of conformity. Page 41

User and service manual AUS124 from sn 101711486

Bio-Optica Milano S.p.A. Via San Faustino 58 - 20134 Milano

Phone +39 02.21.27.13.1 - Fax Italia +39 02.21.53.000 - Fax Export +39 02.21.54.155

www.bio-optica.it – info@bio-optica.it – claims@bio-optica.it

Publication date: 09/04/2018

Rev. 04

1) TECHNICAL FEATURES

Carousel automatic stainer for colouring histological and cytological slides.

Dimensional features

Dimensions (WxDxH):

750x750x650 mm.

Weight:

75 Kg.

Electrical connections

Power supply:

230V~ 50/60Hz.

Power:

120 Watt.

Other connections

Water connections:

Provided pipes for supplying the running water dishes:

N. 1 running water inlet pipe, internal Ø 10 mm, provided with rapid connection with

interception.

N. 1 running water outlet pipe, internal Ø 18 mm.

N. 2 neoprene pipes - internal Ø 10 mm, one metre long - provided with rapid connection

with interception (for the connection between the dishes and the instrument).

Water pressure:

From 2,5 to 4 bar.

Structural features

Painted steel chassis resistant to scratches and solvents. On

the right side are placed: N. 1 green general switch, N. 1 power supply socket with relative fuses, N. 2 connecters

for pipes connecting running water dishes/instrument. On

the left side are placed: N. 1 connecter connecting running water inlet pipe, N. 1 connecter connecting running

water outlet pipe, N. 1 running water pressure regulator, N. 1 filter seat cover provided with handle.

External cover for containing fumes.

Basket holder cover, positioned under the external cover, provided with 24 guides for coupling one or more

baskets and 24 stainless steel covers for covering the dishes.

Central basin for carrying waste water from wash dishes to the drain placed on the left side of the instrument.

N. 4 adjustable rubber feet to get a perfect horizontal position.

Aspiration/filtration system for the treatment of fumes. The instrument is provided with N. 1 active charcoal

cartridge filter specific for alcohol and xilol. Practical filter’s replacement from the left side of the instrument.

Revolving dish holder disk to facilitate the access to the dishes placed on the back side.

User and service manual AUS124 from sn 101711486

Bio-Optica Milano S.p.A. Via San Faustino 58 - 20134 Milano

Phone +39 02.21.27.13.1 - Fax Italia +39 02.21.53.000 - Fax Export +39 02.21.54.155

www.bio-optica.it – info@bio-optica.it – claims@bio-optica.it

Publication date: 09/04/2018

Rev. 04

N. 2 stainless steel slide holder baskets (capacity 65 standard slides 26x76 mm).

N. 21 reagents dishes (capacity 750 ml) in white thermoplastic material.

N. 1 dish (capacity 750 ml) in white thermoplastic material for baskets load/discharge in position 0.

N. 2 running water dishes (capacity 750 ml) in transparent thermoplastic material.

N. 2 independent electric valves which activate the water flow when there is the passage in running water.

Pressure regulator to regulate the water flow.

Working features

General ignition switch.

Emergency switch for stopping all the machine functions at any time.

Entire management of the instrument through use of single colour touch screen panel with intuitive graphics.

Possibility of visualizing steps of program during the execution of the cycle.

Possibility of memorizing more than 999 different protocols of coloration, with maximum number of 48 steps

for each.

Possibility of programming for each station: time of immersion/oscillation and dripping from 00’ : 01’’ up to 59’

: 59’’.

Page of visualization and description of alarms accessible also during the execution of the program.

Possibility of stopping and restarting a program (in case of possible controls, filling up of reagents, ecc.).

Visual and acoustic alarm in case of cycle end.

Visual and acoustic alarm in case of filter saturation.

Visual and acoustic alarm in case of table out of position.

Visual and acoustic alarm for water discharge (malfunction of electric valve, obstruction discharge pipes, etc.).

Possibility of visualizing the steps of the stations during the cycle.

Possibility of programming without leaving the general program page.

Possibility of varying the parameters of the steps also during the execution of the running program.

Possibility of deleting the program steps without leaving the general page.

Visualization of programs time already executed by date and hour.

User and service manual AUS124 from sn 101711486

Bio-Optica Milano S.p.A. Via San Faustino 58 - 20134 Milano

Phone +39 02.21.27.13.1 - Fax Italia +39 02.21.53.000 - Fax Export +39 02.21.54.155

www.bio-optica.it – info@bio-optica.it – claims@bio-optica.it

Publication date: 09/04/2018

Rev. 04

2) INSTALLATION

During the stainer moving, proceed cautiously in order to avoid possible damages.

Position the instrument on a level and stable working bench, free of vibrations. Make sure that on the upper part there

is space enough (ca. 30 cm) to allow the ascent/descent movements of the cover. Regulate the 4 rubber feet to get a

perfect horizontal position. Connect the instrument to the power supply socket (230V~ 50/60Hz) using the provided

cable.

Important:

Do not use any extension or adapter and do not modify the provided cable.

Respect the following working environmental conditions:

Room temperature between 10°C and 40°C.

Relative humidity between 30% and 75%.

Maximum temperature inlet water: 40°C.

3) WATER CONNECTIONS

Inlet running water

Connect the provided pipe (∅10 mm) between the connecter placed on the left side of the instrument and a water tap.

Outlet running water

Connect the provided pipe (∅18 mm) between the connecter placed on the left side of the instrument and the drain of

the laboratory.

Important: As the draining happens by fall, it is necessary that the pipe is positioned as linear as possibile and downhill

(the water outlet connecter of the stainer must be situated over del water outlet connecter of the laboratory).

Connection between the two running water dishes and the instrument

Connect the two provided pipes (∅10 mm) between the connecters of the running water dishes and the connecters

situated on the right side of the instrument. Choose the position of the dishes according to the main programme. Make

sure that the pipes are long enough to rotate the dishes holder disk.

User and service manual AUS124 from sn 101711486

Bio-Optica Milano S.p.A. Via San Faustino 58 - 20134 Milano

Phone +39 02.21.27.13.1 - Fax Italia +39 02.21.53.000 - Fax Export +39 02.21.54.155

www.bio-optica.it – info@bio-optica.it – claims@bio-optica.it

Publication date: 09/04/2018

Rev. 04

Left side of the instrument.

Right side of the instrument.

Water inlet connecter

Water outlet connecter

Pressure regulator

Filter seat

Green

general switch

Connecter for wash

dish N. 2

Connecter for wash

dish N. 1

Power supply

socket

User and service manual AUS124 from sn 101711486

Bio-Optica Milano S.p.A. Via San Faustino 58 - 20134 Milano

Phone +39 02.21.27.13.1 - Fax Italia +39 02.21.53.000 - Fax Export +39 02.21.54.155

www.bio-optica.it – info@bio-optica.it – claims@bio-optica.it

Publication date: 09/04/2018

Rev. 04

Adjustment of the pressure reducer valve

The pressure reducer valve regulates the water flow when the pressure of the plant is irregular or not adjustable.

Instructions for the flow adjustment:

1) In main menu touch key MANUAL CONTROLS.

2) Enable the wash dishes touching keys OFF by WATER 1 and WATER 2.

3) Open abundantly the water.

4) Pull the knob till you hear a click and rotate it till you get the right quantity of water (clockwise to increase the flow,

anticlockwise to reduce it).

5) Finished the adjustment, block the valve by pressing the knob till you hear a click.

6) Disable the wash dishes touching keys 1 or 2 (enabled water green button, disabled water red button).

7) In order to return to menu, touch exit key positioned low on the left.

User and service manual AUS124 from sn 101711486

Bio-Optica Milano S.p.A. Via San Faustino 58 - 20134 Milano

Phone +39 02.21.27.13.1 - Fax Italia +39 02.21.53.000 - Fax Export +39 02.21.54.155

www.bio-optica.it – info@bio-optica.it – claims@bio-optica.it

Publication date: 09/04/2018

Rev. 04

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