Agilent Technologies Bravo Platform User manual

Original Instructions

This guide contains the following topics:

•“About this guide” on page 2

•“Safety guidelines” on page 3

•“Hardware components and axes of motion” on page 4

•“Starting up and shutting down” on page 8

•“Running a protocol” on page 9

•“Stopping or pausing a run” on page 11

•“Using Bravo Diagnostics to control the device” on page 13

•“Changing the Bravo head” on page 17

Bravo Platform

Quick Guide

About this guide

2Bravo Platform Quick Guide

About this guide

This guide summarizes the operator instructions in the Bravo Platform User Guide.

This guide assumes the following:

•The Bravo Platform is installed correctly. For details, see the Bravo Platform Safety

and Installation Guide.

•The device profile for the specific Bravo configuration is already created and the

teachpoints are already set. For setup instructions, see the Bravo Platform User

Guide.

•You are familiar with the VWorks Automation Control software. See the VWorks

Automation Control Quick Start. For detailed instructions, see the VWorks

Automation Control User Guide.

Where to find the user documentation

To access the user guides for Agilent Automation Solutions products, do one of the

following:

•From within VWorks software, select Help > Knowledge Base or press F1.

•From the Windows desktop, select Start > Agilent Technologies >

VWorks Knowledge Base.

•Go to the online VWorks Knowledge Base at www.agilent.com/chem/askb.

Contacting Agilent Technologies

Web: https://www.agilent.com

Contact page: https://www.agilent.com/en/contact-us/page

Documentation feedback: [email protected]

Safety guidelines

Bravo Platform Quick Guide

Safety guidelines

WARNING

Using controls, making adjustments, or performing procedures other than those

specified in the user documentation can expose you to moving-parts hazards and

hazardous voltage. Before using the Bravo Platform, make sure you are aware of the

potential hazards and understand how to avoid being exposed to them.

Ensure that you are trained in the safe operation of the device and that you have read

the Agilent Automation Solutions Products General Safety Guide and the safety section

of the Bravo Platform Safety and Installation Guide.

Figure Safety label locations on Bravo Platform (front view)

The Bravo Platform has moving parts that are accessible at the front, sides, and rear of

the device, if not protected by the Bravo light curtain and shields. For a description of

the moving parts, see “Axes of motion” on page 6.

For the emergency-stop procedure, see “Emergency stops” on page 11.

WARNING

If you touch any of the moving parts or attempt to move labware while the Bravo

Platform is in operation, the device could pinch, pierce, or bruise you. Keep your

fingers, hair, clothing, and jewelry away from the device while it is in motion.

WARNING

The Bravo head z-axis motor is particularly powerful. It might not stop immediately in

a collision and a pipette tip could pierce your hand. Keep away from the Bravo

Platform when the Bravo head is moving or about to move, especially in the z-axis

direction.

Bravo

Hardware components and axes of motion

4Bravo Platform Quick Guide

Hardware components and axes of motion

Primary hardware components

The following figure and table describe the primary hardware components.

Figure Bravo Platform components (front view)

Bravo

Item Feature Description

1Indicator

lights

The two light panels that display color-coded status of the Bravo Platform:

•(solid blue). The Bravo Platform is turned on and in standby mode.

•(flashing green). The software is running a protocol on the Bravo Platform.

•(flashing orange). The software has initialized the Bravo Platform and Bravo

Diagnostics is open.

•(flashing red). The software has encountered an error while running a protocol

or the interlock circuit is tripped.

2Power

switch

The switch on the right side of the rear wall that turns on (I) and off (O) the Bravo

Platform.

Hardware components and axes of motion

Bravo Platform Quick Guide

3Emergency-

stop

pendant

The pendant that contains the red emergency-stop button. To stop in an emergency,

press the red button. All Bravo motion stops.

4Liquid-

handling

head

The platform uses interchangeable Bravo-compatible heads, including disposable-tip,

pin tool, and the Bravo 96AM Head for AssayMAP cartridges.

Figure Series III disposable-tip head

5Gripper An optional gripper that extends from the head mount to below the pipette head tips.

The gripper picks and places labware on the deck.

6Tie bar The vertical bar at the front of the device that adds structural support to the Bravo head

mount. The tie bar moves at high speed from side to side (x-axis) across the front of

the Bravo deck whenever the head moves to a deck location.

7Deck The area that is accessible by the liquid-handling head. The nine labware locations are

numbered 1–3 (back row), 4–6 (middle row), and 7–9 (front row).

Figure Nine deck locations for labware (top view)

Item Feature Description

Hardware components and axes of motion

6Bravo Platform Quick Guide

Axes of motion

The following figures and table show the Bravo moving parts. The Bravo light curtain

and shields prevent operator access to moving-parts hazards.

Figure Bravo Platform primary axes of motion

8Light

curtain and

front and

side shields

The safety equipment that prevents operator access to Bravo moving parts hazards:

•Light curtain. Enables operator access to the Bravo deck while protecting operators

from moving-parts hazards. If an object disrupts the light curtain, the motion of the

Bravo head stops.

•Shields. Clear plastic shields prevent operator access to sections of the Bravo not

covered by the light curtain.

For more details on the Bravo safety equipment components, see the Bravo Platform

Safety and Installation Guide.

Item Feature Description

Item Part Axis of

motion

Description

1Tie bar X The tie bar is attached to the arm and moves side

to side across the front of the deck.

2Arm X The arm carries the head mount side to side across

the deck.

00232

Bravo

Pinch Hazard

00232

Hardware components and axes of motion

Bravo Platform Quick Guide

If the Bravo Platform is fitted with a gripper, the gripper moves with the Bravo head. In

addition, the gripper has the following axes of motion:

•G-axis. The opening and closing distance of the gripper fingers, which enable the

gripper to grip and release labware.

•Zg-axis. The vertical distance the gripper arm moves, which enables the gripper to

extend beyond the pipette head.

Figure Bravo gripper assembly axes of motion

3Head

mount

Y and Z The liquid-handling head attaches to the head

mount, which moves back and forth on the arm

between the back and front of the deck.

The head mount raises and lowers the liquid-

handling head.

Item Part Axis of

motion

Description

Bravo

Starting up and shutting down

8Bravo Platform Quick Guide

Starting up and shutting down

The following procedures describe how to start up and shut down the Bravo Platform

when you are operating it as a standalone device. For instructions on how to turn on

and turn off the Bravo Platform when it is integrated into a workstation or system, see

the workstation or system user documentation.

Starting up the Bravo Platform

To start up the Bravo Platform:

1Turn on any accessories, such as a Pump Module, and ensure that any tubing is

connected. For a description of the Pump Module and autofilling reservoirs, see the

Pump Module User Guide.

2Turn on the computer and the monitor. The Microsoft Windows operating system

starts automatically.

3On the side of the Bravo Platform, press the power switch to the on (I) position.

The green light on the power switch is illuminated when the Bravo Platform is on.

4Start the VWorks software.

Shutting down the Bravo Platform

Shut down the Bravo Platform before you remove or change the pipette head, clean the

Bravo Platform, or perform any routine maintenance.

To shut down the Bravo Platform:

1Make sure that the post-run cleanup procedure was followed after the last run. For

details, see “Cleaning up after the run” on page 10.

2Shut down the computer.

3Turn off any accessories, such as the Pump Module.

4If using an autofilling reservoir, disconnect the bottles to prevent siphoning.

5On the side of the Bravo Platform, press the power switch to the off (o) position.

Bravo

Running a protocol

Bravo Platform Quick Guide

Running a protocol

Preparing for the run

Before you start a run, make sure you review the protocol and ensure the following:

•The correct liquid-handling head is installed. If you need to install or change the

head, see “Changing the Bravo head” on page 17.

•The emergency-stop pendant is connected properly.

•The indicator lights on the front of the Bravo Platform are blue.

•The accessories and labware required for the protocol are where they should be

positioned.

•The reservoirs or wash stations are filled.

•The waste bins or bottles are empty.

If the Bravo Platform is equipped with a Pump Module, the pumps should be primed

before the first run of the day to ensure that the tubing from the source bottle is filled.

Priming is typically part of the Startup Protocol. For information about the Pump

Module, tubing connections, and setup of source and waste bottles, see the Pump

Module User Guide.

Starting a run

WARNING

When you initialize the Bravo Platform, the head and tie bar will move. To prevent

potential injury, keep clear of the device while it is in motion.

You must initialize the Bravo Platform to establish communication between the device

and the computer and to move the components to their home positions. When you

open a protocol in the VWorks software, an initialization message appears

automatically for any devices in the protocol that are not yet initialized.

To start a run:

1In the VWorks software, ensure that simulation is off, and then open the protocol.

2When the Would you like to initialize the devices now? message appears, click Yes.

3If the Verify that it is safe to home the W-axis (the aspirate/dispense axis) message

appears, and the tips do not contain fluid, click Retry to continue homing the w-axis.

If the tips contain fluid, click Ignore and continue. When the W-axis is not homed

message appears, click Diagnostics to open the Bravo Diagnostics software and

use the diagnostics tools to home the w-axis over a waste position. For the homing

procedure, see the Bravo Platform User Guide.

4If the A microplate-in-gripper error message appears, and the gripper is not holding

labware, click Ignore and Continue to continue the homing process.

If the gripper is holding labware, click Abort to cancel the initialization, and then

remove the labware from the gripper. To use Bravo Diagnostics to open the

gripper, see the Bravo Platform User Guide. To restart initialization, open the device

file (.dev), and then click Initialize All Devices in the VWorks window.

5Click Start to start the run.

Running a protocol

10 Bravo Platform Quick Guide

Cleaning up after the run

CAUTION

Use only the recommended cleaning materials. Using other cleaning solutions and

materials can cause damage to the device. Do not use abrasive, corrosive cleaning

agents. Do not use metal brushes.

Use standard laboratory wipes and a mild detergent or ethanol alcohol to clean the

exterior painted white surfaces and the metal surfaces of dust, grime, chemical

deposits, and other debris.

To clean up the Bravo Platform after a run:

1Check the run log file for errors. For details on the VWorks run log, see the VWorks

Automation Control User Guide.

2Ensure that the tips are ready for the next run:

•Disposable-tip pipette head. You can use the Tips Off task in Bravo Diagnostics

to remove any tips that remain on the barrels of the pipette head. For details,

see “Using Bravo Diagnostics to control the device” on page 13.

•Fixed-tip pipette head. You can use the Wash Tips task in Bravo Diagnostics to

wash the pipette tips.

3Ensure all pipette head movement has stopped, and then remove any manually

placed or unused labware, and clean up any spills or debris.

4Wash the liquid reservoirs and wash stations.

5If equipped with a Pump Module:

aOptional. Wash the tubing and reinstall the reservoirs or wash stations. Ensure

that the tubing is connected to the correct pumps.

bFill the fluid reservoir bottle, replace the cap, and attach the fluid line that

pumps towards the Bravo Platform to the cap connector.

cEmpty the waste container, replace the cap, and attach the fluid line that

pumps away from the Bravo Platform to the cap connector.

For connection details, see the Pump Module User Guide.

6If equipped with a Weigh Station, recalibrate it if you are changing the tubing

connected to the reservoir, changing the liquid type used in the reservoir, or if more

than two weeks have elapsed since the last Weigh Station calibration. For details,

see the Bravo Platform User Guide.

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