Zeiss Axiovert 100 User manual

3D Microscopy for

Axiovert 100/135/135 M

Operating Manual

2B 40-022-4 e 07/98

Knowledge of this manual is required for the operation of the instrument. Would you therefore

please make yourself familiar with the contents of this manual and pay special attention to hints

concerning the safe operation of the instrument.

The specifications are subject to change.



Unless expressly authorized, forwarding and duplication of this document, and the utilization and

communication of its contents are not permitted. Violations will entail an obligation to pay

compensation.

Issued by: Carl Zeiss

Mikroskopie

D-07740 Jena

Telephone: (03641) 64-16 16

Telefax: (03641) 64-31 44

Internet: mikro @ zeiss.de

http://www.zeiss.de

Number of this manual: B 40-022-4 e

Date of issue: 07/98

3D Microscopy Contents

B 40-022-4 e 07/98 3

Contents

1 Notes on safety ................................................................................................... 4

1.1 General.............................................................................................................................. 4

1.2 Regulations concerning instrument safety and electromagnetic compatibility (EMC)........... 4

1.3 Notes on unpacking, transport and storage........................................................................ 5

1.4 Hints on use....................................................................................................................... 5

1.5 Notes on maintenance....................................................................................................... 5

2 General................................................................................................................. 6

2.1 Application ....................................................................................................................... VI

2.2 Function............................................................................................................................. 7

2.2.1 Eyepiece version................................................................................................................. 7

2.2.2 Monitor version.................................................................................................................. 8

2.2.3 Combination of eyepiece and monitor version ................................................................ 10

2.3 Maintenance and care ..................................................................................................... 11

2.3.1 General............................................................................................................................ 11

2.3.2 Cleaning of optical components....................................................................................... 11

2.3.3 Change of voltage and fuses............................................................................................ 11

2.3.4 Troubleshooting............................................................................................................... 12

3 Technical Data ................................................................................................... 13

Certification in accordance with DIN EN ISO 9001 and DIN EN 46001

EC Declaration of Conformity

Annex (optional)

3D microscopy for Axiotron 2

3D microscopy for Axiotech 100

3D microscopy for Axiotech 100 vario

3D microscopy for Axiovert 100/135/135 M

3D microscopy for Axiovert 25 CA

3D microscopy for Axioplan 2/Axiophot 2

3D microscopy for Axioskop

3D microscopy for Axioskop 2

Notes on safety 3D Microscopy

4B 40-022-4 e 07/98

1 Notes on safety

1.1 General

Please make yourself familiar with the contents

of these operating instructions before

attaching and starting up the components for

3D microscopy. Additional information is

available from our maintenance service or from

authorized agencies.

For the function of 3D microscopy in

combination with the



Axiotron 2



Axiotech 100



Axiotech 100 vario



Axiovert 100/135/135 M



Axiovert 25 CA



Axioplan 2/Axiophot 2



Axioskop



Axioskop 2

please see the operating instructions of these

microscopes.

When using video components from KAPPA,

please comply with the supplied manual "Color

TV camera for microscopy and 3D images".

Special regard must be paid to the notes on

instrument safety included in this manual.

To guarantee the safe operation and function

of the components for 3D microscopy, it is

necessary under any circumstances to take the

precaution measures and observe the warnings

contained in the operating instructions.

The following symbols are used for the

warnings:

Caution!

Non-observance of the safety notes constitutes

a hazard for the user.

Caution!

Non-observance of the safety notes constitutes

a hazard for the instrument.

Caution!

Disconnect the instrument from the line before

opening it.

☞Note!

Notes which must be observed.

1.2 Regulations concerning

instrument safety and

electromagnetic

compatibility (EMC)

The components for 3D microscopy have been

designed and tested in compliance with EN

61010, Part 1 (VDE 0411). From the safety

viewpoint, they left the factory in a perfect

state.

They meet the requirements of the EC directive

89/336/EC and the EMC legislation of

November 9th 1992 and the legal regulations

for accident prevention.

The components for 3D microscopy meet the

EC requirements and are CE-labelled.

3D Microscopy Notes on unpacking, transport and storage

B 40-022-4 e 07/98 5

1.3 Notes on unpacking,

transport and storage

The components for 3D microscopy must be

transported in their original packaging. It is

recommended to use the original packaging

also for storage.

The transport and storage temperatures must

comply with those listed under "Technical

Data".

When unpacking the components which are

equipped with polarizing foil (3D illumination

shutter, eyepieces with 3D shutter), make sure

to be particularly careful, since the foil can be

easily scratched.

1.4 Hints on use



The components for 3D microscopy can

only be used with halogen lamps up to

max. 100 W.



The power plug must be inserted in a

socket featuring a grounding (earth)

contact.

The grounding effect must not be reduced

by an extension cable which does not have

a protective ground wire.



Prior to connection to the line, check

whether the voltage set on the selector at

the rear of the 3D-CONTROL DAS-3 unit

complies with the line voltage; it may be

necessary to change from 230 V to 115 V.

The change to 115 is only required for the

3D-CONTROL DSA-3 unit.

For further details, see "Care and

Maintenance" on page VIII.



Cable connections may only be made if the

various components are switched off.



For the video components from KAPPA, it is

absolutely necessary to use only the

connectors listed in the manual "Color TV

camera for microscopy and 3D images".



Make sure that the cables are not bent or

laid out in too narrow a radius.



The optical components must be protected

from dirt and mechanical damage;

special care must be taken to prevent the

polarizing foils of the 3D illumination

shutter from being touched.



The components for 3D microscopy must be

protected from impact and humidity.

1.5 Notes on maintenance



To enable 3D microscopy to function

perfectly, it is necessary to use only

components and spares marketed or

admitted by us. When in doubt, please

contact our service staff.



With the exception of the work described in

the "Care and maintenance" chapter, the

user must not perform any repairs or

changes himself. We would like to

emphasize that all other changes and

repairs must be performed by specially

authorized personnel.



The manufacturer is not liable for damage

caused by unauthorized operation. This

forfeits all the claims against warranty.

General

Application 3D Microscopy

6B 40-022-4 e 07/98

2 General

2.1 Application

3D microscopy permits the stereoscopic viewing

of microscope specimens and objects



up to highest magnifications



using all objectives



eyepiece and monitor observation



in reflected and transmitted light



on upright and inverted microscopes



in brightfield and phase contrast



in real color



in real time



with extended depth of focus



with improved resolution



storage via recorder or image memory of the



documentation via video prints or color

photos

The following microscopes can be upgraded

with the components for 3D microscopy:



Axiotron 2



Axiotech 100



Axiotech 100 vario



Axiovert 100/135/135 M



Axiovert 25 CA



Axioplan 2/Axiophot 2

3D microscopy is ideal for the following

applications in biomedicine:



micromanipulation



patch-clamp technique



in-vitro fertilization



examination of endocytosis and xenobiotics



examination of phagocytosis



injection of nucleotides



examination of nerve cells

3D microscopy is ideal for the following

applications in materials microscopy:



defect analysis of semiconductor

components



observation of surface topography in

microtechniques



materials examinations in construction

engineering



rupture analysis



examination of the structure of microfibers



examination of the structure of crystalline

materials



examination of compound materials.



Axioskop



Axioskop 2

3D Microscopy Function



Eypiece version

B 40-022-4 e 07/98 7

2.2 Function

The dynamic technique on which 3D

microscopy is based can be used with any light

microscope with an accessible aperture

diaphragm.

The technique can be used with upright or

inverted reflected-light and transmitted-light

microscopes.

A patent has been applied for the technique.

1 Eyepiece with 3D-Shutter

2 3D-CONTROL DSA-3

3 3D illuminator shutter

4 Objective

5 Object plane

Eyepiece version

in a reflected-light microscope

There are three different versions of 3D

microscopy:



eyepiece version



monitor version



combination of eyepiece and monitor

version

2.2.1 Eyepiece version

An LCD shutter ("3D illumination shutter") is

positioned close to the aperture diaphragm and

is used to open or close one half of the beam

path.

With the eyepiece version, the LCD shutter is

controlled via the "3D-CONTROL DSA-3" unit.

The centroid of the illumination beam path is

switched in such a way that the object is

illuminated alternately from a right and a left

stereo angle.

The eyepiece shutters are controlled at the

same pulse rate, i.e. each eye only sees the

image on its own side.

right eye



right image

left eye



left image

A flicker-free display of the stereo image

composed of the right and left images is made

possible by an appropriately high pulse rate.

When the illuminator - eye allocation is

changed, a pseudostereo effect is created

(height-changed image).

Resolution, contrast and depth can be

optimized with relation to the object via the

aperture diaphragm.

Function



Monitor version 3D Microscopy

8B 40-022-4 e 07/98

1 Eyepiece with 3D-Shutter

2 3D-CONTROL DSA-3

3 Depolarizer

4 3D illumination shutter

5 Condenser

6 Object plane

7 Objective

Eyepiece version

in a transmitted-light microscope

The depolarizer is required for minimization of

disturbances by the polarization equipment and

facilitates work with birefringent objects

2.2.2 Monitor version

An LCD shutter ("3D illumination shutter") is

positioned close to the aperture diaphragm and

is used to open or close half a side of the beam

path.

With the monitor version, the LCD shutter is

controlled via the "3MCU II" unit.

The centroid of the illumination beam path is

switched in such a way that the object is

illuminated alternately from a right and a left

stereo angle.

The camera and the monitor shutters are

controlled at the same pulse rate, i.e. each eye

only sees the image on its own side.

right eye



right image

left eye



left image

In the monitor version, allocation is made via

the display of the half images in two

perpendicular polarization directions which can

be allocated to the eyes by polarizing glasses.

A flicker-free display of the stereo image

composed of the right and left images is made

possible by an appropriately high pulse rate.

When the illuminator - eye allocation is

changed, a pseudostereo effect is created

(height-changed images).

Resolution, contrast and depth can be

optimized with relation to the object via the

aperture diaphragm.

3D Microscopy Function



Monitor version

B 40-022-4 e 07/98 9

1 Monitor shutter

2 Glasses

3 Control unit for monitor shutter

4 MCU II image memory

5 3D illumination shutter

6 Objective

7 Object plane

8 Camera

Monitor version

in a reflected-light microscope

1 Monitor shutter

2 Polarizing glasses

3 Camera

4 Objective

5 Object plane

6 Condenser

7 3D illumination shutter

8 MCU II image memory

9 Control unit for monitor shutter

Monitor version

in a transmitted-light microscope

Function



Combined version 3D Microscopy

10 B 40-022-4 e 07/98

1 Monitor shutter

2 Polarizing glasses

3 Camera

4 Control unit for monitor shutter

5 MCU II image memory

6 3D-CONTROL DSA-3

7 3D illumination shutter

8 Depolarizer

9 Object plane

10 Eyepiece with 3D Shutter

Combined version

in a transmitted-light microscope

2.2.3 Combination

of eyepiece and monitor version

An LCD shutter ("3D illumination shutter") is

positioned close to the aperture diaphragm and

is used to open or close one half of the beam

path.

With the combined version, the LCD shutter is

controlled via the "3D-CONTROL DSA-3" unit.

The centroid of the illumination beam path is

switched in such a way that the object is

illuminated alternately from a right and a left

stereo angle.

The eyepiece shutter and the camera and

monitor shutters are controlled at the same

pulse rate, i.e. each eye only sees the image

on its own side.

right eye



right image

left eye



left image

In the monitor version, allocation is made via

the display of the half images in two

perpendicular polarization directions which can

be allocated to the eyes by polarizing glasses.

A flicker-free display of the stereo image

composed of the right and left images is made

possible by an appropriately high pulse rate.

When the illuminator - eye allocation is

changed, a pseudostereo effect is created

(height-changed image).

Resolution, contrast and depth can be

optimized with relation to the object via the

aperture diaphragm.

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