Zeiss 880 Installation guide

Zeiss 880

Training Notes

Zen 2.3

Turn on

Main Power

Switch

Do you need the Argon

Laser

458, 488, or 514 nm lines?

Yes

Turn on the Systems PC

Switch

Turn on the HXP 120V Lamp

Turn on the Components

Switch.

Turn on the PC and log into

your account.

Start Zeiss Zen via Elan

Tracking Software.

No

Create

scanning

configuration

880 Start-Up Sequence

Set up sample on microscope.

Go to the Acquisition Tab and

initiate the warm up

procedure for the laser.

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2

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Logging into the 780

•Log into the computer with your UCHC or

CAM credentials; be sure you are logging

into the proper domain.

•Double click on the Zen icon, the one

labeled with “AUT-Zeiss LSM 880” or

similar text.

The idea elan tracking and billing software will

initialize after clicking on the Zen icon.

•Enter your UCHC email. Once it has been

recognized, it will bypass the password

requirement and proceed to the session

confirmation window.

Starting Zen with idea elan

•Select your scheduled appointment

and/or click the login button as directed

by the software.

•Confirm your appointment and the Zen

software will be initialized.

Starting Zen 2.3

Select Start System from the Zen 2012 login

window. This will start up the hardware and

software.

Image Processing is for using the system in an

offline mode and this will ignore all the

hardware components.

Introducing the Zen Workspace

The Zen workspace is designed in a

tabular fashion allowing you to

step you through initially looking

at your specimen via the Oculars

tab, in the control panel, to

acquiring images via the Acquire

tab. The remaining tabs add off-

line Processing and

Maintenance options.

Panels can be moved around

to customize the workspace.

Introducing the Zen workspace

Your imaging configuration and your

work space configuration can be

stored separately.

In addition, your imaging configuration

and acquisition parameters can easily be

retrieved from the Reuse function when

you have a previously saved image loaded

in the workspace.

Getting Light to the Oculars

Locate Tab

1. Click on the objective icon to access the list

of possible objectives. Be sure to note the

immersion type; air, oil or water. Please do

not change from oil to air/dry or from

water to oil and oil to water without

cleaning your specimen first.

2. Select your light source from:

Transmitted ON

Transmitted OFF

DAPI

FITC

Rhodamine

3. Be sure to turn the Reflected Light “Off”

when you are finished looking at your

specimen.

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Getting all the tools

Acquisition Tab

In order to view all the options available via the software,

be sure to go to:

1. View>Show All (global)

2. Select >Show all Tools.

Make sure to do this each time you use the software

otherwise the required features, like the lasers, will not

be accessible to you.

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Open/close the

submenu with the arrow.

Click the arrow to detach/attach panel.

Acquisition Tab

1. Experiment Manager

The manager allows you to save your scan configuration

settings for your experiment and reload the settings for

a quick start. This method does not record the

acquisition parameters.

2. Scan Control Tool Bar

•AF (Find Focus) – computer determines focus based

on contrast settings.

•Set Exposure – the computer automatically

calculates the gain and black level settings.

•Live – initiates a fast scan regardless of the scan

speed and continuously scans until Stop is selected.

•Continuous – continuously scans according to the

selected scan speed until Stop is selected.

•Snap – performs a single scan according to the

selected scan speed.

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