YSI YSI 5905 User manual

YSI 5905/5010
BOD Probe
Instruction
Manual


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Table of Contents
INTRODUCTION........................................................................ 1
OPERATING PRINCIPALS......................................................... 2
USING THE 5905/5010 BOD PROBE....................................... 3
CHANGING MEMBRANE CAPS - FIGURE 2 ............................ 3
WARM UP............................................................................ 5
ELECTRODE CLEANING - FIGURE 3....................................... 5
OPERATING PROCEDURES AND PRECAUTIONS....................... 7
PROBLEM SOLVING................................................................. 9
TROUBLE SHOOTING GUIDE ................................................. 9
SERVICING............................................................................. 11
WARRANTY AND REPAIR ...................................................... 13
CLEANING INSTRUCTIONS .................................................. 15
PACKING INSTRUCTIONS..................................................... 16
PROBE SPECIFICATIONS........................................................ 18
APPENDIX A........................................................................... 19
APPENDIX B........................................................................... 20

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1
INTRODUCTION
The YSI Model 5905/5010 BOD (Biochemical Oxygen
Demand) probe is used for measuring dissolved oxygen in all
popular size BOD bottles. This probe features self stirring, an
easily replaced membrane cap, and a refurbishable electrode
system.
The 5905 probe is designed for use with all YSI dissolved
oxygen meters, except for the Model 55 or Model 95. It must
be used with an adapter (5011) in order to be used on the
Model 5000 and 5100.
The 5010 probe is specifically designed for use with the Model
5000 and 5100 only.
This manual also applies to probes 5905-J, 5905-K, 5905-L,
5905-W (Appendix A), and probes 5010-J, 5010-K, 5010-L,
and 5010-W (Appendix A)
IMPORTANT
Intended Use
The 5905/5010 probe is intended for laboratory use, not field
use. It is not waterproof and should never be immersed past
the taper on the stem of the probe.

2
OPERATING PRINCIPALS
The 5905/5010 BOD probe is a voltammetric (polarographic)
sensor of dissolved oxygen. An oxygen permeable membrane
covers an electrolytic cell consisting of a cathode and an
anode. This membrane acts as a diffusion barrier and an
isolation barrier preventing the fouling of the cathode surface
by impurities in the environment. The cathode is the gold
electrode, and the anode is the silver electrode which
completes the electrolytic cell and acts as a reference
electrode.
Upon entering the cell through the membrane, oxygen is
reduced at an applied potential of -0.8 V referenced to the
silver electrode. The reduction current at the cathode is
directly proportional to the partial pressure of oxygen in liquid
(expressed as %-air saturation) which is also proportional to
the concentration of dissolved oxygen (in mg/L) at a particular
temperature. Thus the same partial pressure of oxygen (%-air
saturation) in liquid gives different concentrations of dissolved
oxygen (mg/L) at different temperatures because of the
different solubilities of oxygen at different temperatures.

3
USING THE 5905/5010 BOD PROBE
Note: The 5905 must be plugged into a YSI meter and the
stirrer power plug must be plugged into its power adapter. The
5010 must be plugged into a YSI meter only, as it gets its
stirring power from the 5000/5100 instrument.
CHANGING MEMBRANE CAPS - FIGURE 2
Follow these instructions to change membrane caps:
1. Remove the stir paddle from the probe by pulling it straight
out.
2. Unscrew the old membrane cap from the probe. Before
installing a new membrane, clean the probe tip with
deionized water in order to remove any contaminants.
3. Hold the membrane cap and fill it at least ½ full with the
electrolyte solution provided. Screw the membrane cap onto
the probe moderately tight. A small amount of electrolyte
should overflow.
4. Rinse off excess electrolyte from the probe with deionized
water.
5. Reinstall the stir paddle.
New Probe Use
New probes are shipped with a dry membrane cap to protect
the electrodes. A new membrane cap must be installed
before first use.

4
Fill Membrane Cap
with Solution
Unscrew Cap
Remove
Stir Paddle
Screw Cap on
moderately tight
Replace
Stir Paddle
Figure 2 Membrane Installation on the 5905/5010 Probe.

5
WARM UP
Every time the meter is turned on, the 5905/5010 BOD Probe
must be allowed to warm up for about 30 minutes before
taking the first reading. This allows the probe to use up all the
oxygen retained in the cap.
New Probe Break In (Motor Off)
ELECTRODE CLEANING - FIGURE 3
The 5905/5010 should be cleaned only when erratic readings
occur or after about every 500 hours of use (two months).
Each cleaning removes material and reduces the life of the
probe, so excessive cleaning should be avoided.
Gold Cathode:
For correct probe operation, the gold cathode must be textured
properly. It can become tarnished or plated with silver after
extended use. The gold cathode can be cleaned by using the
adhesive backed sanding disc provided in the 5906 Membrane
Kit. Stick the disc to a small flat object, like a bottle cap, then
sand the gold with a twisting motion about 3 times or until all
silver deposits are removed and the gold appears to have a
For best results, please allow the probe more than the usual 30
minutes to warm-up after the first membrane is installed. The
process may be faster if the probe is allowed to warm up in a
BOD bottle filled with clean water. If possible, it’s a good idea
to let new probes warm-up over night (with a new membrane
and KCl) prior to their first use.

6
matte finish. If the cathode remains tarnished, return the probe
for service.
Cathode
(Gold)
Temperature
Sensor
Anode
(Silver)
Figure 3 5905/5010 Sensor
Silver Anode:
It is normal for a dark layer of silver chloride to cover the
silver anode. After prolonged use it may become necessary to
clean the anode. Soak the probe in a 14% ammonium
hydroxide solution for 2 to 3 minutes or overnight in a 3%
ammonium hydroxide solution. Rinse with deionized water,
recharge with electrolyte, and install a new membrane.
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