Swift STELLAR 1 Series User manual

SHOP.SWIFTOPTICAL.COM

STELLAR 1 SERIES

Biological Microscope

Instruction Manual

M TEC LIMITED

We are constantly endeavouring to improve our instruments and

to adapt them to the requirements of modern research techniques

and testing methods. This involves modication to the mechanical

structure and optical design of our instruments.

Therefore, all descriptions and illustrations in this instruction

manual, including all specications are subject to change without

notice.

Microscope terminology

Abbe Condenser

A two-lens sub-stage condenser

located below the stage of a

microscope and functions to

collect light and direct it onto

the object being examined. Its

high numerical aperture makes

it particularly suited for use

with most medium- and high-

magnication objectives.

Aperture, Numerical (N.A.)

The numerical aperture is an

important factor determining the

e󰀩ciency of the condenser and

objective. It is represented by the

formula: (N.A. = ηsinα), where

η is the refractive index of a

medium (air, water, immersion oil

etc.) between the objective and

the specimen or condenser, and

α is half of the maximum angle

at which light enters or leaves

the lens from or to a focused

object point on the optical axis.

Cover Glass Thickness

Transmitted light objectives are

designed to image specimens

that are covered by a thin cover

glass (cover slip). The thickness

of this small glass piece is now

standardized at 0.17 mm for

most applications.

Diaphragm, Condenser

A diaphragm, which controls the

e󰀨ective size of the condenser

aperture. A synonym for the

condenser illuminating aperture

diaphragm.

Magnication

The number of times by which the

size of the image exceeds the original

object. Lateral magnication is usually

meant. It is the ratio of the distance

between two points in the image

to the distance between the two

corresponding points in the object.

Micrometer (um)

A metric unit of length measurement

= 1x10-6 meters or 0.000001 meters

Nanometer (nm)

A unit of length in the metric system

equal to 10-9 meters.

Phase–contrast (microscopy)

A form of microscopy, which converts

di󰀨erences in object thickness and

refractive index into di󰀨erences in

image amplitude and intensity.

Real Vieweld

The diameter in millimetres of the

object eld.

Eyepiece Field of View

Real Vieweld =

Objective Magnication

For example Stellar 1:

Eyepiece eld of view = 10mm

Objective magnication = 10X

Diameter of the object eld = 18/10

= 1.8mm

Diopter Adjustment

The adjustment of the eyepiece

of an instrument to provide

accommodation for the eyesight

di󰀨erences of individual

observers.

Depth of Focus

The axial depth of the space on

both sides of the image plane

within which the image is sharp.

The larger the N.A. of objective,

the shallower the depth of focus.

Field of View (F.O.V.)

That part of the image eld,

which is imaged on the

observer’s retina, and hence can

be viewed at any one time. The

eld of view number is now one

of the standard markings of the

eyepiece.

Filter

Filters are optical elements

that selectively transmit light.

It may absorb part of the

spectrum, or reduce overhaul

intensity or transmit only specic

wavelengths.

Immersion Oil

Any liquid occupying the

space between the object and

microscope objective. Such

a liquid is usually required by

objectives of 3-mm focal length

or less.

Resolving Power

A measure of an optical system's

ability to produce an image

which separates two points or

parallel lines on the object.

Resolution

The result of displaying ne details in

an image

Total Magnication

The total magnication of a microscope

is the individual magnifying power of

the objective multiplied by that of the

eyepiece.

Working Distance

This is the distance between the

objective front lens and the top of the

cover glass when the specimen is in

focus. In most instances, the working

distance of an objective decreases as

magnication increases.

X–axis Stage Travel

The axis that is usually horizontal in a

two-dimensional coordinate system.

In microscopy X-axis of the specimen

stages is considered that which runs

left to right.

Y–axis Stage Travel

The axis that is usually vertical in a

two-dimensional coordinate system.

In microscopy Y-axis of the specimen

stages is considered that which runs

front to back.

Table of contents

I Nomenclature

II Setting up the instrument

Operating Enviroment

III Assembling the microscope

1. Verifying input voltage

2. Illumination

3. Mechanical stage

4. Objectives

5. Condenser

6. Eyepiece tube

7. Eyepieces

8. Filters

IV Usage of microscope compoents

1. Illumination brightness adjustment

2. Interpupillary distance adjustment

3. Diopter adjustment

4. Coarse and fine focusing

9. Use of Darkfield

5. Use of Parfocal guide line

6. Coarse focus torque adjustment

7. Stage upper limit stop adjustment

8. Use of condenser aperture diaphragm

10. Use of Phase Contrast (Phase Contrast models only)

V Photomicrographic procedure

VI Using of oil immersion objectives

VII Maintenance and storage

VIII Troubleshooting table

IX Quality warranty

Darkfield

I. Nomenclature

Stage

Eyepiece

Diopter

Adjustment Ring

Objective

Coarse Focus Knob

Fine Focus Knob

Stage X-Y Travel Knob

Light Intensity Control

Binocular

Eyepiece Tube

Objective Color Ring

Color Coded Aperture

Diaphragm

Transmitted Light

Collector

Specimen Holder lever

Eyepiece Tube

Clamp Screw

Power Adapter

Compartment

Brightness Indicator

Revolving Nosepiece

Condenser Aperture lever

Slide Storage

Power Cord Hanger

Parfocal Guide Line

Coarse Focus

Torque Adjustment

Fine Focus

Coarse Focus

Filter Holder

Photo Port

Stellar 1-T

II. Setting up the instrument

Avoid placing the instrument in locations exposed to direct sunlight, dust,

vibration, high temperature and high humidity.

Operating environment

● Indoor use

● Altitude: Max 2000 meters

● Ambient temperature: 15°C to 35°C

● Maximum relative humidity: 75% for temperature up to 31°C decreasing

linearly to 50% relative humidity at 40°C

● Supply voltage uctuations: Not to exceed ±10% of the normal voltage.

● Pollution degree: 2 (in according with IEC60664)

● Installation / Overvoltage category: 2 (in according with IEC60664)

● Air pressure of 75kPa to 106 kPa

● Avoid frost, dew, percolating water, and rain

III. Assembling the microscope

1. Verifying input voltage

● The 5V microscope input can works with a broad range of USB power

sources. However, always use the provided automatic voltage selection

power adapter or own adapter, portable power device that are rated for

the voltage used in your area, microscope input and that has been

approved to meet local safety standards.

● In order to prevent electric shock, always turn the power switch on the

power supply o󰀨 before connecting the power cord.

● Electrical specications:

AC adapter: 100-240V 50/60 Hz (Included)

Microscope Input: Type-C 5V/2A by AC adapter or portable power device

(not included)

2. Illumination

● LED illumination

LED Illumination Specication:

3.3V 1W, color temperature 5300K ~ 6000K

LED Power connection:

- Take out power adapter from the power adapter compartment on the

rear of microscope and power cord from the styrofoam packaging box.

- Connect the Type-C socket of the of the power cord to the inlet on the

rear of the microscope base. Plug in the other end of the cord to power

adapter and connect the adapter to an AC outlet.

● Reection mirror (Optional)

The mirror serves to be used for microscopic observation using natural

light in a location where power supply is not available.

- For this purpose, it is necessary to remove the transmitted light collector.

- Raise the stage up to the top position. Rotate the light collector in anti-

clock direction and remove it from microscope base. Rotate the reection

mirror into the microscope base and incline the mirror until the daylight is

reected homogeneously into the light path. Please use the concave face

of the mirror when natural light is not strong enough.

3. Mechanical stage

● Specimen holder has been attached in the stage by using the two

mounting holes.

●Open the specimen holding lever of the specimen holder, set the

specimen by sliding it on the stage from the front toward the rear. After

setting the specimen, return the specimen holding lever gently

●Remove specimen holder for fast hand scanning of slides

4. Objectives

● Objectives are installed in revolving nosepiece. If necessary to reinstall,

lower the stage completely, screw the objectives into the revolving

nosepiece so that clockwise rotation of the nosepiece brings the next

higher magnication objective into position.

5. Condenser

● The condenser is mounted in the stage and it is used in conjunction

with the iris diaphragm. The function of the condenser is to provide

full illumination to the specimen plane and to enhance the resolution and

contrast of the object being viewed.

6. Eyepiece tube

● Eyepiece tube is preinstalled in microscope arm. If necessary to reinstall,

loosen the eyepiece tube clamp screw, insert the round dovetail mount

on the eyepiece tube into the round dovetail mount on the microscope

arm, tighten the eyepiece tube clamp screw to secure the eyepiece tube

in place.

7. Eyepieces

● Use the same magnication eyepieces for both the eyes.

● Insert each eyepiece completely into the eyepiece sleeve.

● Twist the eyepiece (anti-clockwise or clockwise) with 20-30 degree and

put the eyepieces gently out when removing the eyepiece.

8. Filters

● The swing-in and swing-out lter holder is under Abbe condenser. Swing

it out and place 32mm diameter lter into it, taking care that dust, dirt and

ngerprints do not get on the lter and the eld lens.

●A di󰀨user is built into the condenser of the microscope.

IV. Usage of microscope components

1. Illumination brightness adjustment

● Turn the light intensity control wheel fully clockwise to the low brightness

position.

● Set the power switch on the rear of microscope to position “I” (ON)

● The brightness indicator of Swift Logo in the front of microscope base will

light up.

● When the light intensity control wheel is turned anti-clockwise to high

brightness position, the light intensity will increase.

●Automatic power o󰀨 function:

A safety and environmentally friendly feature in Stellar 1. If the brightness

is unchanged for 30 minutes, the microscope illumination will

automatically switch o󰀨. Turn the light intensity control wheel and the

illumination will automatically recover.

2. Interpupillary distance adjustment

● Before adjusting the interpupillary distance, bring a specimen into focus

using the 10x objective.

● Adjust the interpupillary distance so that both the right and left eld of

view become one.

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