Richter Optica U2 Manual

info@richter-optica.com

Setting up your Richter Optica U2 Microscope for simple phase

contrast observations

Shown below is the simple phase contrast kit used on the Richter Optica model U2

Microscope. It consists of one 40x Phase Contrast objective lenses, a centering

telescope and a phase slider.

To install:

•First attach the 40x phase lens into the nosepiece

•Next, you’ll need to remove the existing plug in the condenser and insert the

phase slider in its place.

•Not all phase contrast microscopes are the same but generally they rely on

similar techniques to set up the system for optimum results. In the system

shown below, the condenser has 5 settings, 4x, 10x 40x, 100x, PH. 4x-100x

are for your brightfield settings with no Phase. Move your slider to the PH

setting when using the 40x Phase lens.

To begin observation:

•To set use your microscope for phase contrast, first set the condenser at BF for

4x and focus on the specimen. Then do the same with the 10x. Make sure

you are adjusting the height of the condenser for optimum image quality.

•Next, set the condenser turret to the phase setting.

•Rotate the 40x Phase lens into the optical path and adjust if necessary on the

specimen.

•Pull the phase slider out away from the microscope until you hear it click into

place on the 40x setting.

•Next, you will need to remove one of the eyepiece lenses and insert the

centering telescope in its place. The set screw is used to focus the centering

telescope.

•When looking through the centering telescope, you will see two rings. They

may or may not be concentric. By using a small allen wrench, you center the

40x ring on the phase slider so that the rings you see when looking through the

telescope are concentric (see the image below).

Image once aligned and optimized for Phase Contrast

•Finally, remove the centering telescope and replace the eyepiece lens. Put

your specimen back on the stage and you are ready for phase contrast

observations.

Table of contents

Other Richter Optica Microscope manuals

Richter Optica

Richter Optica UX1B User manual

Richter Optica

Richter Optica S2 User manual

Richter Optica

Richter Optica S-6.6-BL User manual

Richter Optica

Richter Optica S6-CL User manual

Richter Optica

Richter Optica HDCAM3 User manual

Richter Optica

Richter Optica HS-2M User manual

Richter Optica

Richter Optica S6-BL User manual

Richter Optica

Richter Optica U-1B User manual

Richter Optica

Richter Optica MDS2 User manual

Richter Optica

Richter Optica HS-3M User manual

Richter Optica

Richter Optica HS-1M User manual

Richter Optica

Richter Optica MDS1-D User manual

Richter Optica

Richter Optica MDS1 User manual

Richter Optica

Richter Optica HSX1 User manual

Richter Optica

Richter Optica S6-HD User manual

Richter Optica

Richter Optica F-1 User manual

Richter Optica

Richter Optica HDCAM3B User manual

Popular Microscope manuals by other brands

Thermo Scientific

Thermo Scientific Nicolet iN10 Using

National

National DC3-163 instructions

KERN Optics

KERN Optics OPE-1 User instructions

OPTIKA MICROSCOPES

OPTIKA MICROSCOPES B-150D Operation manual

Prior

Prior Lux POL operating instructions

Opitec

Opitec Smartscoop 115615 instructions

KERN

KERN OPO-1 operating instructions

Topcon

Topcon SP-1P user manual

Motic

Motic PA43 Operation manual

Leica

Leica DVM5000 Setup manual

MshOt

MshOt MZ62 user manual

KERN Optics

KERN Optics OZR-5 User instructions

VWR

VWR VisiScope 384 Series instruction manual

Nikon

Nikon ECLIPSE E200 POL instructions

Leica

Leica DI C800 User's manual & installation instructions

ThermoFisher Scientific

ThermoFisher Scientific Continuµm manual

ThermoFisher Scientific

ThermoFisher Scientific Continuµm manual

Olympus

Olympus SZ61 instructions