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In microscopes with 400x magnification, the objectives are al-
ready attached to the nosepiece.
For the microscopes with 600x and 1000x magnification, the
60x and 100x objectives are not yet attached to the revolving
nosepiece, but are part of the scope of delivery.
Check that all the objectives are firmly attached. To do this,
grasp the knurled rings of the objectives with your fingers and
turn them to the right. Do not use a tool to tighten them. The
entire nosepiece with the 3 or 4 objectives can be turned by
hand. When you rotate the nosepiece, you will notice that the
objectives click into place. They do so in the correct position
relative to the optical axis of the microscope.
Object table with preparation clamps or mechanical stage
Object table/stage with preparation clamps: The micro slide is
locked in place with the two clamps. It is then carefully posi-
tioned with the fingers.
Mechanical stage: The microscope slide is placed in the spec-
imen holder of the mechanical stage and can be moved in X
and Y direction. The stage has a working area of 140 x 130
mm, the travel range is 78 x 30 mm.
Coarse and fine adjustment
The focussing mechanisms for coarse and fine adjustment are
located on one axis (coaxial). On the fine adjustment knobs
there is a graduation. This can be used to measure the depth
of a specimen.
When the stage automatically slides down after using the mi-
croscope for some time, turn the adjustment ring on the inside
of the coarse and fine adjustment knobs slightly in the direction
of the arrow. The coarse adjustment knob is tightened to pre-
vent the stage from sliding down.
Abbe condenser with iris diaphragm
Below the object stage is an Abbe condenser N.A. 1.25. The
condenser can be adjusted in height by turning. This allows
light to be focused on the specimen for contrast enhancement.
The condenser is already pre-centered.
The iris diaphragm with filter holder is located under the con-
denser. The light intensity can be adjusted by changing the
movable opening.
Lighting
The power-saving illumination is the reason why the micro-
scopes of the MIC-100 series do not need to be connected to
the mains all the time for operation, but can also be operated
independently of the mains, as they contain a rechargeable
NiMH battery. The illumination is a modern LED lamp with the
following specifications:
LED: 3W, brightness adjustable
Power supply: 100V-230V
5 WORKING WITH THE MICROSCOPE
Please follow the following instructions to achieve the best re-
sults:
Adjustment of interpupillary distance and diopter dis-
tance for a binocular model
First adjust the coarse and fine adjustment knobs so that you
get a sharp microscope image with the right eyepiece (A1),
and then adjust the diopter adjustment ring (A3) on the left
eyepiece tube (A2) to get a sharp image with the left eyepiece
as well. Finally, adjust the interpupillary distance (A4) so that
it corresponds to the viewer's interpupillary distance. Some-
times the microscope head (A5) should be rotated to obtain a
comfortable observation position instead of moving the micro-
scope body.
This procedure must be carried out anew for each user.
Adjusting the lighting
For optimal contrast and resolution, please follow these steps:
Place a micro slide on the stage and focus with the 4x lens.
The iris diaphragm is open.
Turn the condenser to the highest position.
Close the iris diaphragm until it is just visible at the edge of
the image field.
The microscope is now optimized for use with the 4x ob-
jective. Repeat this procedure with each additional magni-
fication to achieve the best balance between contrast and
resolution.
Attention: the maximum light intensity of the microscope
can damage your eyes if you use it with the 4x and 10x
objectives!
If you swap microslides, start again with the 4x objective.
It is recommended to use slides of 1.0 to 1.2 mm thickness in
combination with cover glasses of 0.13 mm or 0.17 mm thick-
ness.
Battery
The microscope contains a 3.6V NiMH battery.
A charging indicator is located on the side of the microscope.
It is red when connected to the power supply and green when
the battery is full. If the red indicator flickers, the microscope is
not properly connected to the power supply.
The microscope's illumination can be powered by a battery
pack for 4 to 8 hours without an external power supply.
Using the S100x oil immersion objective
The MIC-119A and MIC-129A are equipped with an S100x
N.A. 1.25 oil immersion lens. Please follow the following
recommendations for using this lens:
Focus the image with the S40x lens.
Rotate the revolving nosepiece until the S100x objective
almost (not quite) clicks into place.
Drop a small drop of immersion oil (not part of the scope of
delivery, please order separately) on the center of the
specimen.
Then let the S100x objective snap into place.
The front lens is now in contact with the immersion oil.
View the specimen through the eyepiece and focus the im-
age with the fine adjustment knob.
The distance between objective lens and specimen is only
0.14mm!
If small bubbles are visible, turn the S100x objective a few
times to the left and right so that the front lens of the objec-
tive moves in the oil. This will make the bubbles disappear.
Then turn the stage with the fine adjustment knob down-
wards until the front lens does not touch the oil anymore.
Always clean the front lens of the S100x lens with a piece
of lens paper moistened with a drop of xylene or alcohol.
Now also clean the microslide.
