Nikon E800 User manual

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Nikon

Microscope

ECLIPSE

E800

Instructions

I

Thank

you

for

purchasing

this

Nikon

product.

This

instruction

manual

is

written

for

users

of

the

Nikon

Microscope

ECLIPSE

E800.

To

ensure

corret

usage

read

this

manual

carefully

before

operating

the

instrument.

•

It

is

prohibited

to

alter

this

manual

in

part

or

whole

without

expressed

permission.

•

The

contents

of

this

manual

are

subject

to

change

without

any

notice.

•

Although

every

effort

has

been

made

to

ensure

the

accuracy

of

this

manual,

if

you

note

any

points

that

are

unclear

or

incorret,

contact

your

nearest

Nikon

representative.

•

Also

be

sure

to

read

the

instruction

manual

for

the

power

supply

that

you

are

using,

either

the

V-PS100DU,

or

the

V-PS100E.

Warning/Caution

Symbols

in

This

Manual

Though

Nikon

products

are

designed

to

provide

you

utmost

safety

during

use,

incorrect

usage

or

disregard

of

the

instructions

can

cause

personal

injury

or

property

damage.

For

your

safety,

read

the

instruction

manual

carefully

and

thoroughly

before

usage.

Do

not

discard

this

manual

but

keep

it

near

the

product

for

easy

reference.

Inside

this

instruction

manual,

safety

instructions

are

indicated

with

the

symbols

shown

be¬

low.

Be

sure

to

follow

the

instructions

marked

with

these

symbols

tor

your

safety.

Symbol

Disregarding

instructions

marked

with

this

symbol

may

lead

to

death

or

serious

injury.

Disregarding

instructions

marked

with

this

symbol

may

lead

to

injury

or

property

damage

.

CAUTION

1.

Check

the

light

source

The

power

supplies

V-PS

100DU

and

V-PS100E

provide

the

power

for

the

halogen

lamp

that

serves

as

the

microscope

light

source.

Although

these

power

supplies

can

power

a-halogen

lamp

of

up

to

12

V/

100

W,

the

specified

lamp

and

lamphouse

must

be

used.

The

equipment

could

be

damaged

if

a

non-

specified

combination

of

components

is

used.

Specified

lamphouse

:

Nikon

halogen

lamphouse

Model

C-LP

HALOGEN

12V100W

Specified

lamp

:

Halogen

lamp,

12

V-100

W

LONGLIFE

OSRAM

HLX

64623

or

PHILIPS

7724

2.

Turn

off

the

power

when

connecting

or

disconnecting

cables

or

when

replacing

the

lamp

In

order

to

prevent

electric

shock

and

damage

to

the

equipment,

always

turn

the

power

switch

on

the

power

supply

off

(flip

it

to

the

“

”

side)

and

unplug

the

power

cord

before

connecting

or

disconnecting

cables

or

replacing

the

lamp.

3.

Cautions

when

replacing

the

lamp

In

order

to

prevent

bums,

allow

the

lamp

to

cool

(for

at

least

thirty

minutes

after

turning

off

the

power)

before

replacing

the

lamp.

Furthermore,

in

order

to

prevent

electric

shock

and

damage

to

the

equipment,

always

turn

the

power

switch

on

the

power

supply

off

(flip

it

to

the

~

”

side)

and

unplug

the

power

cord

before

replacing

the

lamp.

After

replacing

the

lamp,

make

sure

that

the

lamphouse

cover

is

securely

closed.

Never

use

the

lamphouse

while

its

cover

is

open.

4.

Do

not

wet

If

the

microscope

or

the

power

supply

becomes

wet,

a

short

circuit

may

result

and

the

equipment

could

be

damaged

or

could

become

extremely

hot.

If

you

accidentally

spill

a

liquid

on

the

equipment,

immediately

turn

the

power

sw

itch

on

the

power

supply

off

(flip

it

to

the

“

”

side)

and

unplug

the

power

cord.

Then

use

a

dry'

cloth

to

wipe

away

the

moisture.

If

any

liquid

gets

inside

of

the

equipment,

do

not

attempt

to

use

it;

instead,

contact

your

nearest

Nikon

representative.

5.

Weak

electromagnetic

waves

This

microscope

emits

weak

electromagnetic

waves.

The

accuracy

of

any

precision

electronic

equipment

may

be

adversely

affected

if

positioned

near

this

microscope.

If

this

microscope

affects

TV

or

radio

reception,

move

the

radio

or

TV

away

from

the

microscope.

6.

Cautions

concerning

installation

Be

careful

not

to

pinch

your

hands

or

fingers

when

installing

the

microscope.

This

microscope

is

a

precision

optical

instrument,

and

using

or

storing

it

under

unsuitable

conditions

may

damage

it

or

may

have

an

adverse

affect

on

its

precision.

See

“Installation

Location"

on

P.27,

and

use

this

microscope

in

a

suitable

environment.

7.

Notes

on

transport

Because

this

microscope

is

a

precision

optical

device,

handle

it

carefully

and

do

not

subject

it

to

strong

physical

shocks.

(The

precision

of

the

objective

in

particular

may

be

adversely

affected

by

even

weak

physical

shocks.)

When

moving

the

microscope,

first

detach

the

lamphouse.

Then

have

at

least

two

people

carry

the

microscope,

at

least

one

on

each

side,

using

the

carrying

handles.

Be

sure

to

screw

the

carrying

handles

in

as

far

as

they

will

go

and

make

sure

that

they

are

secure.

If

the

carrying

handles

are

not

attached

securely,

they

could

come

out

or

break

while

the

microscope

is

being

carried,

creating

a

hazardous

situation.

Screw

the

carrying

hadles

into

the

upper

holes

on

the

rear

of

the

microscope.

If

you

use

the

lower

holes,

your

hands

will

be

caught

between

the

handles

and

the

table

when

you

place

the

microscope

down.

(P.27)

In

addition,

do

not

grasp

the

coarse

focus

knob,

the

fine

focus

knob,

the

eyepiece

tube,

the

stage,

etc.,

when

carrying

the

microscope;

there

is

a

chance

that

the

part

could

come

off,

and

the

equipment

could

be

damaged.

Names

of

Component

Parts

2.

Names

of

Operational

Parts

3.

Microscopic

Procedure

3

Set

the

brightness

adjuster

to

“9”.

a

This

brightness

provides

the

best

color

reproduc¬

tion.

(p.

23)

■■

Insert

filters

ND32

and

HUI

NCB11

into

the

optical

j|||l|

ND32:

Reduces

glare

in

Jlljl

the

binocular

eyepiece.

f||||

NCB11:

Improves

color

•iSra

reproduction,

(p.

21)

Lower

ND32

and

NCB11

Set

to

“9\

I

Move

the

lOx

objective

into

the

optical

path,

d

Rotate

the

revolving

^

nosepiece

until

the

lOx

3

objective

clicks

into

place.

Set

the

specimen

in

place

with

the

cover

glass

facing

Cover

glass

facing

up

la

\=r-

Select

lOx

9

i

;

3.

Microscopic

Procedure

3.

Microscopic

Procedure

Switch

to

any

desired

objective

and

view

the

specimen.

•

Re-adjust

the

focus.

•

Use

ND

filters

to

adjust

the

brightness.

•

Close

the

field

diaphragm

so

that

it

is

just

outside

the

field

of

view.

•

Close

the

condenser

aperture

diaphragm

to

about

70

to

80%

of

the

objective

numerical

aperture,

(p.

19)

■

If

a

portion

of

the

field

of

view

is

dark,

try

adjusting

the

condenser

centering

screws.

If

that

does

not

resolve

the

problem,

check

the

following

items:

•

Filter

insertion/removal

(p.

21)

•

Positioning

of

the

revolving

nosepiece

(p.

35)

•

Condenser

focusing

(p.

18)

•

Full

opening

of

the

field

and

aperturediaphragms

(p.

19,

20)

•

Optical

path

selection

for

the

eyepiece

tube

(p.

16)

•

Lamp

installation

(p.

28)

•

Revolving

nosepiece

installation

(p.

30)

•

Condenser

installation

(p.

30)

■

If

it

is

not

possible

to

focus

on

the

specimen,

check

the

following

items.

•

Mounting

of

the

specimen

(p.

35,

36)

•

Release

of

the

coarse

focus

stopper

(p.

15)

•

Thickness

of

the

cover

glass

(0.17

mm

is

standard)

(p.

35)

4.

Operation

of

Each

Part

Coarse

focus

stopper

The

coarse

focus

stopper

marks

the

stage

position

at

which

the

specimen

is

in

focus

by

restricting

the

movement

of

the

coarse

focus

knob.

(Movement

of

the

stage

by

the

fine

focus

knob

is

not

restricted.)

Once

the

coarse

focus

stopper

is

clamped

in

place,

the

coarse

focus

knob

cannot

be

used

to

move

the

stage

any

higher.

In

effect,

once

the

coarse

focus

knob

is

clamped

in

place

at

the

focus

position,

a

rough

focus

can

be

attained

the

next

time

simply

by

turning

the

coarse

focus

knob

as

far

as

it

will

go.

This

feature

is

convenient

when

viewing

similar

specimens

one

after

another.

If

the

coarse

focus

stopper

is

not

being

used,

the

coarse

focus

stopper

ring

should

be

loosened

as

far

as

it

will

go

(by

turning

it

as

far

as

it

will

go

in

the

direction

opposite

to

that

indicated

by

the

arrow

on

the

base

of

the

microscope.)

I

Using

the

coarse

focus

stopper

With

the

specimen

in

focus,

turn

the

coarse

focus

stopper

ring

as

far

as

it

will

go

in

the

direction

indicated

by

the

arrow

on

the

base

of

the

microscope

(about

270°).

The

coarse

focus

stopper

is

now

clamped

in

place.

When

changing

the

specimen,

lower

the

stage

by

turning

only

the

coarse

focus

knob.

After

changing

the

specimen,

gently

raise

the

stage

by

turning

only

the

coarse

focus

knob

as

far

as

it

will

go.

The

specimen

should

be

roughly

in

focus

when

the

stage

has

been

raised

as

far

as

it

will

go;

use

the

fine

focus

knob

to

bring

the

specimen

into

perfect

focus.

4.

Operation

of

Each

Part

Diopter

adjustment

method

1

Perform

steps

H

to

E

in

chapter

"3.

Microscopic

Procedure,”

so

that

the

specimen

is

fo¬

cused

with

the

lOx

objective.

2

Turn

the

diopter

adjustment

rings

on

the

eyepieces

and

align

the

edges

of

the

diopter

adjust¬

ment

rings

with

the

engraved

lines.

(This

is

the

diopter

adjustment

0

position.)

3

Move

the

40x

objective

into

the

optical

path.

Turn

the

coarse

and

fine

focus

knobs

to

focus

on

the

specimen.

4

Move

the

4x

or

the

lOx

objective

into

the

optical

path.

5

Turn

the

diopter

adjustment

rings

on

the

eyepieces,

not

the

coarse

and

fine

focus

knobs,

and

focus

on

the

specimen.

Do

so

while

peering

through

the

right

eyepiece

with

your

right

eye

and

the

left

eyepiece

with

your

left

eye.

6

Repeat

steps

3

to

5

twice.

I*]

Interpupillary

distance

adjustment

r--s

Before

adjusting

the

interpupillary

distance,

perform

steps

D

to

El

in

chapter

“3.

Microscopic

Pro-

:1

cedure”

so

that

the

specimen

is

focused

with

the

lOx

objective.

■

Adjust

the

interpupillary

distance

so

that

the

field

of

view

for

each

eye

is

aligned

on

one

position.

Doin°

so

will

make

observation

through

the

binocular

eyepiece

with

both

eyes

easier.

M

V'-M