Merck Milliflex Oasis User manual
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Notice
The information provided in this document (or any other literature) is subject to change without prior notice
and shall not constitute a commitment of Merck KGaA, Darmstadt, Germany or any of it’s Aliates. Merck
KGaA, Darmstadt, Germany or any of it’s Aliates expressly decline all warranties and shall not assume any
responsibility for any errors that may appear in this document. This manual is believed to be complete and
accurate at the time of publication. In no event shall Merck KGaA, Darmstadt, Germany or any of its Aliates be
liable for all damages (including but not limited to incidental or consequential damages) in connection with, or
arising from, the use of this document or associated literature.
Table of Contents
Introduction 3
1. Components 4
1.1 Required items 4
1.2 Description of items 5
1.3 Touch switch 6
2. Hardware installation 7
3. Testing 9
3.1 Filtration units 9
3.2 Media plates 9
3.3 Testing 9
3.4 Waste 10
4. Cleaning & maintenance 11
4.1 Cleaning & maintenance table 11
4.2 Surface sanitizers and internal ow
sanitizers table 11
4.3 Filtration head cleaning 12
4.4 Pump surface cleaning 12
4.5 Pump rinsing 12
4.6 Filtration head external gasket cleaning 12
4.7 Pump internal ow sanitization 13
4.8 Filtration head complete cleaning 14
4.9 Filtration head gaskets and check valve
(spare parts) replacement 15
4.10 Sanitization kit gaskets and valve
(spare parts) replacement 16
5. Performance verication 17
6. Use of multiple pumps 18
6.1 Material needed 18
6.1.1 Two pumps connected 18
6.1.2 Three pumps connected 18
6.2 Installation 18
7. System specications 20
8. Ordering information 21
9. Troubleshooting 23
10. Operator & equipment safety instructions 23
11. Technical assistance 23
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Introduction
The Milliex Oasis®pump for the EZ-Fit® ltration unit is a solution for the microbial testing
of aqueous uids.
The system is composed of the Milliex Oasis®pump, the EZ-Fit® ltration unit and culture media.
Please refer to the ordering information section for details on the dierent items.
EZ-Fit® ltration unit:
The EZ-Fit® ltration unit is a disposable ltration device for bioburden testing of liquid
samples including water, in-process samples, and nal products.
The EZ-Fit® ltration units have been designed to ensure optimal microbial recovery. The
sterilized, disposable ltration devices for bioburden testing streamline your workow,
save time and give you highly reliable microbial enumeration results. The blue and pink
drain support and the funnel-to-base assembly have been optimized so that the membrane
remains perfectly at after the sample ltration so that when transferred to the culture
medium, the whole ltration surface of the membrane is in perfect contact with the culture
media. The shape of the funnel and the hydrophobic material minimize sample residue
ensuring that the complete sample volume reaches the membrane surface. The innovative
funnel assembly design prevents any leaks or sample by-pass of the membrane, meaning
that the whole sample volume is ltered. EZ-Fit® ltration units conform to international
standards (EP/USP) and comply with water testing regulations.
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1. Components
1.1 Required items
Three hardware item numbers are necessary to run the system:
1. Milliex Oasis®pump for EZ-Fit® ltration unit (item MMSYSTEZ1)
2. Milliex Oasis® power supply for one to three ltration pumps, specic to
dened regions:
• Australia: item MMPWRSPAU
• Brazil: item MMPWRSPBR
• China: item MMPWRSPCN
• Denmark: item MMPWRSPDK
• Europe: item MMPWRSPEU
• Switzerland: item MMPWRSPSZ
• India: item MMPWRSPIN
• UK: item MMPWRSPUK
• Japan: item MMPWRSPJP
• South Africa: item MMPWRSPZA
3. Milliex Oasis® internal ow sanitization kit (item MMSANKIT1)
Optional: For higher throughput capability, it is possible to connect two or
three pumps together without additional power supplies. In this case, the
Milliex Oasis®electrical cable for connecting two pumps (item MMCABLEMM)
is required per additional pump.
Consumables: please refer to the ordering information section for details on
the dierent items.
1. Filtration units
2. Culture media
3. Consumables for the Milliex Oasis® internal ow sanitization kit
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1.2 Item description
The Milliex Oasis®pump for
EZ-Fit® ltration unit package
(item MMSYSTEZ1) contains
1. Pump body
2. Two ltration heads with their covers
3. Tubing for discarding the liquids
4. Accessory kit
5. Spare parts kit
The Milliex Oasis®power supply
package contains
1. Power supply
2. Power cable (region specic)
3. Two rubber fasteners
The Milliex Oasis® internal ow
sanitization kit (item MMSANKIT1)
package contains:
1. Stainless steel sanitization kit with
plug and gasket removal tool
2. Vacuum gauge
The EZ-Fit® ltration unit package
contains 48 units.
Culture media: please refer to the
ordering information section to
select your preferred media.
The Milliex Oasis®consumable for
internal ow sanitization kit (item
MMSANSYFU) package contains:
1. Twenty-four plastic syringes for
dispensing the sanitizer
2. Twenty-four funnel assemblies for
rinsing with puried water
Figure 1: Milliex Oasis®pump for EZ-Fit® ltration
unit (MMSYSTEZ1) package components
Figure 2: Milliex Oasis® power supply package
components
Figure 3: Milliex Oasis® internal ow sanitization
kit package components
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1.3 Touch switch
Color Meaning
White Steady: pump is ready for next handling step
Blinking: membrane dry out on-going
Blue Vacuum on
Green The sanitization kit is ready to use
Red No ltration head or ltration head is not locked
Orange Blinking: synchronizing multiple pumps, wait
O No tension or standby
The switch is touch-sensitive. Use your
ngertip to activate it.
Touch the center of the switch without
touching the outer ring.
When the Milliex Oasis®pump is in standby
mode, it can be reactivated by touching the
space between the two switches.
Figure 4: INCORRECT—no activation if the nger
touches the outer ring
Figure 5: CORRECT nger position to start or stop
the pump
✓
✗
Note: If unused for thirty minutes, the
pump switches o. Touch the switch
once to power up.
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2. Hardware installation
The pump must be installed on a horizontal
surface. Please make sure that your working
area is level to ensure good sample volume
readings.
• Take the pump body, pump heads and
tubing out of the package with care
• Take out the accessory bag and spare
part bag
Optional: the accessory bag contains four
stickers; select your preferred sticker and
stick it carefully into the specific groove on
the pump.
Figure 6: Pump sticker set up
• Clean the exterior of the pump and the
tubing using a wipe moistened with a
surface sanitizer listed in the cleaning &
maintenance section
• Place the pump in the working area
Under a horizontal laminar flow hood (LFH),
the front of the pump should be placed at
least at 160 mm (6.3 in.) from the front
edge of the LFH (see picture).
Figure 7: The front of the pump should be placed
at least at 160 mm (6.3 in.) from the front edge of
a horizontal LFH
• On the back of the pump, press the metal
latch and remove the white plug, then
connect the tubing
Figure 8: The metal latch must be pressed to
remove the white plug
• Place the other end of the tubing in a
liquid waste container
• Take out the power supply out of its
package
• Sanitize the power supply in the same
way as the pump
• Plug the DC power cord to one power port
situated at the back of the pump. Push
the connectors into the power port until
a click is heard
• Connect the free power cord to the
power supply
• Connect the other end of the power cord
to a properly grounded power outlet
Optional: attach the power supply outside
the working area with the two rubber
fasteners.
Figure 9: The power supply can be attached
outside the working area with the rubbers
fasteners
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Optional: to arrange the tubing and
electrical cable in your working area, use
the Milliflex Oasis®guides for electrical
cable and tubing (item MMTUGUIDE).
Figure 10: Use of Milliflex Oasis®guides for
electrical cable and tubing (item MMTUGUIDE)
• Sanitize the internal flow of the pump
referring to the cleaning & maintenance
section
• Once the internal flow path sanitization
has been performed, take the two pump
heads out of their package and remove
the yellow covers
• Clean the pump heads using a wipe
moistened with a surface sanitizer listed
in the cleaning & maintenance section
• Install the pump heads on the pump body
• Lock the pump heads by turning
clockwise until the sign on the head is
aligned with the one on the pump body
Figure 11: The sign on a locked head is aligned
with the one on the pump body
The pump is ready for testing.
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3. Testing
The following components are needed
before starting your sample processing:
• Milliflex Oasis®pump for EZ-Fit®filtration
unit installed following the installation
section
• EZ-Fit®filtration units
• Culture media
• Samples to test
Ensure that the internal pump flow was
sanitized according to the cleaning &
maintenance section.
• Clean the exterior of the pump using a
wipe moistened with a surface sanitizer
listed in the cleaning & maintenance
section
3.1 Filtration units
Different types of filtration units are
available (see ordering information section)
Note: for testing samples with
antimicrobial activity, select filtration
units with low binding Durapore®
membrane.
Note: Isopropyl myristate (IPM) is not
chemically compatible, therefore sample
containing IPM should not be used.
• Open a box of filtration units and remove
the desired numbers of bags or filtration
units
• Repeat the operation if more than one
box of filtration units is needed.
• Clean each bag or each unit using a wipe
moistened with a surface sanitizer
3.2 Media plates
Follow the instructions of use for your
selected culture media
3.3 Testing
When the pump is on standby mode,
the switch is not lit up. To power up the
Milliex Oasis®pump, touch the front of the
pump between the two switches with your
ngertip.
Note: If starting a filtration without a
filtration unit placed on the filtration
head, you will hear a whistle noise, this
is due to a check valve to be found in
each pump head and is perfectly normal.
If the pump is started after having
placed a filtration unit on the pump head,
this noise will not be heard.
• Remove the device from the packaging by
grasping the funnel
Figure 12: Removing the unit from its packaging
• Remove the plug from the bottom of
the base (blue unit bulk packaging
versions only)
Figure 13: Removing the plug from the unit
(blue unit bulk packaging versions only)
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• Place the EZ-Fit®filtration unit on the
pump head
Figure 14: Filtration unit placement on the
pump head
• Remove the funnel’s cover and pour
the sample into the unit
• Place the cover back in place
• Touch the switch to start the filtration
Note: touch the switch three times within
one second to launch the ltration on
two ltration heads simultaneously.
• Open a culture media plate.
• When the liquid is totally filtered, touch
the switch once more to stop the filtration
• After a few seconds, the pump stops,
and the switch light stops blinking then
remove the cover and slightly pinch the
funnel and remove it as shown here:
Figure 15: Funnel removal
• Remove the membrane using forceps.
Figure 16: Membrane removal
• Place the membrane onto the culture
media plate, close the plate, it is ready
for incubation
Figure 17: Membrane transfer onto culture media
• Remove the base from the filtration head
as shown here:
Figure 18: Filtration unit base removal
Note: If some ltered liquid remains in
the pump head, it is possible to remove
it with a dry wipe. Regardless of the
amount of liquid, it does not impact the
test results.
3.4 Waste
After usage, the funnels can be stacked to
reduce waste volume.
Please refer to the waste management
document available on our website to
recycle the materials.
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