Leica FluoCombi III User manual
2
Leica FluoCombi III™ – User manual
Dear User,
Thank you for your confidence in our products. We trust you will enjoy them
and we wish you great success with the high-quality and high-performance
products from Leica Microsystems.
In developing our instruments, we place great value on simple, self-
explanatory operation. Nevertheless, please take the time to read the oper-
ating instructions so that you know the advantages and possibilities offered
by your stereomicroscope and can use them most efficiently. Should you
ever have any questions, please consult your local Leica representative.
We are gladly at your service. Customer service is a big thing with us. Not
only before the sale, but afterwards as well. You will find the address of
your nearest local representative, as well as valuable information about
Leica Microsystems products and services, from Leica Microsystems on
our web site at www.leica-microsystems.com.
Leica Microsystems (Switzerland) Ltd.
Stereo & Macroscope Systems
www.stereomicroscopy.com
3Leica FluoCombi III™ – Overviews
Overviews
Operating and function elements
1Fine focusing with mount thread for a 5×or 20×HR objective (requires an adapter)
2Clamping screw for locking the fine focusing
3Mechanism for parcentric swinging to the stereo or micro mode
4Slot for filter slide for dichroic mirror of your choice
5Mount for UV protection screen
Fig. 1 Leica FluoCombi IIITM, 5×planapo HR objective rotated into the beam path
9
3
4
7
1
6
5
8
4
6Clamping screw for fastening the UV protection screen
7Clamping screw for locking the UV protection screen in the desired tilt position
8Clamping screw for fastening the optics carrier in the microscope carrier of the FluoCombi III™
9Clamping screw for fastening the focusing stop
Leica FluoCombi III™ – Overviews
Fig. 2 Leica FluoCombi IIITM, 1×planapo objective rotated into the beam path
2
8
6
7
5
9
3
1
5
Leica FluoCombi III™ – User manual
Contents
Page
Overviews . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Operating and function elements . . . . . . . . . . . . 3, 4
1. General notes . . . . . . . . . . . . . . . . . . . . . . . . . . 6
1.1 The user manual . . . . . . . . . . . . . . . . . . . . . . . . 6
1.1.1 Diagrams . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
1.2 Description . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
2. Assembly
2.1 Expansion diagram . . . . . . . . . . . . . . . . . . . . . . 7
2.2 Standard delivery . . . . . . . . . . . . . . . . . . . . . . . 8
2.2.1 Additional components . . . . . . . . . . . . . . . . . . 8
2.3 Assembly sequence . . . . . . . . . . . . . . . . . . . . . 9
2.4 Column with focusing drive
and base of stand . . . . . . . . . . . . . . . . . . . . . . 10
2.5 Focusing stop . . . . . . . . . . . . . . . . . . . . . . . . . 10
2.6 Transport anchorage . . . . . . . . . . . . . . . . . . . 11
2.7 FluoCombi III™ and focusing drive . . . . . . . 11
2.8 Optics carrier . . . . . . . . . . . . . . . . . . . . . . . . . 12
2.9 Objectives . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
2.10 UV protection screen . . . . . . . . . . . . . . . . . . . 14
2.11 Dichroic mirrors . . . . . . . . . . . . . . . . . . . . . . . 14
2.12 Illumination . . . . . . . . . . . . . . . . . . . . . . . . . . . .14
3. Operation
3.1 Parfocality . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
3.2 Magnification . . . . . . . . . . . . . . . . . . . . . . . . . 17
4. Appendix
4.1 Weights . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
4.2 Dimensions . . . . . . . . . . . . . . . . . . . . . . . . . . .19
4.3 Optical data . . . . . . . . . . . . . . . . . . . . . . . . . . .22
4.4 Technical data . . . . . . . . . . . . . . . . . . . . . . . . .24
Leica FluoCombi III™ – General notes
6
1. General notes
1.1 The user manual
Leica FluoCombi III™ is an accessory for the
Leica MZ16 F, MZ16 FA fluorescence stereomi-
croscopes. Together with your Leica FluoCombi III™,
you receive an interactive CD-ROM with the user
manual for all products from Leica Microsystems
(Switzerland) in the current EU languages. User
manuals and updates are also available for you
to download and print from our web site at
www.stereomicroscopy.com. The following
user manuals are important for your
Leica FluoCombi III™:
– M2-166-2 Leica FluoCombi III™: It describes the
specific functions of the FluoCombi III™
– M2-160-4 for MZ16 FA and M2-160-5 for MZ16 F
describe the functions of the motorized and
the manual version
– M2-216-1 describes the lamp housing and
its functions
– M2-105-0 Leica M Stereomicroscopes: It
contains all the detailed information about the
use of the stereomicroscope, the optical data
with stereo objectives, and the safety and care
instructions
– M1-267-1 Leica motor focus system: describes
the motorized focusing
Read the user manuals listed
above before the startup procedure.
In particular, please follow the safety
instructions.
To maintain the unit in its original condition and to
ensure safe operation, the user must follow the
instructions and warnings contained in these user
manuals.
1.1.1 Diagrams
Pages 3 and 4 contain figures showing the
Leica FluoCombi III™ with the function elements.
Numbers in parentheses within the descriptions
refer to the number of the figure and the items in
the figure.
Example (1.4): Figure 1is located on page 3, and
the item 4is the insert for dichroic mirrors.
1.2 Description
Leica FluoCombi III™ is an accessory for the Leica
MZ16 F, MZ16 FA fluorescence stereomicroscopes
and allows for quickly switching between a stereo
objective and an HR (High Resolution) micro
objective. In stereo mode, the 1×plan and planapo
objectives offer generous viewing fields, large
working distances and an excellent depth of field
for manipulating and dissecting.
Micro mode allows for exactly identifying the
finest fluorescent features at a microscopic reso-
lution of 1500 pairs of lines/mm. The zoom is also
effective in micro mode so that the magnification
measures 460×using the 5×HR objective. Using
16×eyepieces results in a maximum magnification
of 736×. The comfortable binocular observation is
ensured in stereo and micro mode.
MZ16 F and MZ16 FA are high-performance stereo-
microscopes for fluorescence applications. The
patented, separate TripleBeam™ light path for
fluorescence illumination and the patented
FLUOIII™ filter system provide highest-quality
fluorescence images. Every existing instrument
can be fitted with the Leica FluoCombi III™ in a
few simple steps.
7
2.1 Expansion diagram
Leica FluoCombi III™ – Assembly
2. Assembly
10 445 631
10 445 367 HL
10 446 352 HL RCTM
10 445 363
10 445 387
10 445 822
10 446 229
Filter
10 447 284
10 447 285
10 447 286
10 447 287
10 447 288
10 447 289
10 446 271
10 447 185 500mm
Motor focus
10 447 041 500mm
10 447 160
10 445 301
10 445 302
10 445 303
MZ16 F
10 447 064
106z
11 504 066
11 504 069
FluoCombi IIITM
10 447 324
MZ16 FA
10 447 063
10 447 243
5x HR
10 447 338
10 447 085 20x HR
Optem / Mitutoyo
10 447 290
10 447 291
10 447 292
10 447 293
10 447 294
10 447 295
10 445 819
10 447 157
Plan/Planapo
8Leica FluoCombi III™ – Assembly
2.2 Leica FluoCombi III™ standard delivery
– Leica FluoCombi III™ with microscope carrier
(Fig. 3) for the MZ16 F and MZ16 FA optics car-
rier, mechanism for swinging into the stereo or
micro mode, fine focusing with mount thread
for an HR objective and for a 1× plan or planapo
objective
– UV protection screen (7.11)
– Focusing stop with stops (7.3)
– Column adapter (7.2a)
2.2.1 Additional components
– HR planapo objective 5×/0.5 LWD (Fig. 4),
FAA 19mm or
– Adapter (5.1) for Mitutoyo/Optem objectives and
screw-in adapter (5.2) as well as the
corresponding objective
– Fluorescence stereomicroscope (7.5) with third
TripleBeam™ beam path, FLUOIII™ filter sys-
tem, rapid filter changer and Leica lamp hous-
ing for mercury high-pressure lamps as well as
binocular tube, eyepieces, stand and focusing
drive
–1×Plan or planapo objective (7.9)
– Dichroic mirror of your choice (Fig. 6)
Assembly and setup of Leica MZ16 F, MZ16 FA
fluorescence stereomicroscope and the fluores-
cence illumination are described in the respective
operating instructions (see p. 6).
Binocular tube, eyepieces, stands, mercury vapor
lamp, etc. are described in the operating instruc-
tions M2-105-0, M2-160-5, M2-160-4, M2-216-1.
Fig. 6 Dichroic mirror
Fig. 5 Adapter (1) for Mitutoyo/Optem objectives
and screw-in adapter (2)
Fig. 4 HR planapo objective 5×/0.5 LWD
Fig. 3 Leica FluoCombi IIITM
1
2
9Plan or planapo objective 1×
10 HR planapo objective 5×(or adapter and
Mitutoyo/Optem objective)
11 UV protection screen
12 Filter sets upon demand in the filter
changer and analogous with a dichroic filter
in the FluoCombi III™
13 Photo equipment (if available)
14 Gliding stage or thermal stage (if available)
9
2.3 Assembly sequence
1Base of stand
2Focusing drive with column (manual or
motorized) and column adapter (2a)
3Focusing stop
4Leica FluoCombi III™
5Optics carrier
6Binocular or video/photo tube and eyepieces
7Lamp housing with high-pressure lamp and
stray-light protection
8Supply unit
Leica FluoCombi III™ – Assembly
Fig. 7 Leica MZ16 F with Leica FluoCombi III™
7
2
2a
3
14
8
6
13
1
12
5
4
11
9
10
Leica FluoCombi III™ – Assembly
2.5 Focusing stop
The focusing stop is used for safety
purposes and must always be correctly set with
respect to the micro objective.
Fasten the focusing stop (8.2) to the column
with the clamping screw (8.3).
Set the focusing stop at a height so that
the micro objective can never hit the stand or
table surface or the object during focusing and
swinging. Working distance 5×HR objective:
19mm, 20×HR objective 13mm.
With a correctly set focusing stop, the two
stops prevent focusing too low, either manually or
motorized.
If the focusing stop is set too low
– the objective could be damaged.
– the object could be crushed or damaged.
– if the lowest focus position is approached too
quickly using the motor focus, the fingers of the
operator could be pinched between objective
and object.
Fig. 9 Leica FluoCombi III™ from below
1Transport safety screws
Fig. 8
1Column adapter
2Focusing stop
3Clamping screw for fastening
the focusing stop
11
2.4 Column with focusing drive ➜base of
stand
First, attach the column adapter between stand
column and base. It is used to ensure that the
center of the objective lies exactly over the center
of the stage.
Fasten the column adapter (8.1) with the
3 hexagon-head screws on the column.
Fasten the column with the column adapter on
the stand base according to the operating
instructions M2-105-0.
1
2
3
10
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