Hoefer HE33 User manual
user
manual
nucleic acid electrophoresis
um
HE33-IM/Rev. H0/05-04
Hoefer HE 33
mini submarine electrophoresis unit
Page nder
Mini submarine electrophoresis unit function
. .
1
Important information
. . . . . . . . . . . . . . . . . . .
2
Unpacking
. . . . . . . . . . . . . . . . . . . . . . . . . . .
4
Speci cations
. . . . . . . . . . . . . . . . . . . . . . . .
4
Operating instructions
. . . . . . . . . . . . . . . . . .
5
Care and maintenance
. . . . . . . . . . . . . . . . .
11
Troubleshooting
. . . . . . . . . . . . . . . . . . . . . .
12
Notes, buffers, and volumes
. . . . . . . . . . . . .
13
Bibliography
. . . . . . . . . . . . . . . . . . . . . . . . .
17
Ordering information
. . . . . . . . . . . . . . . . . . .
18
•
pii
•
p1
Fig 1.
Main components.
(See Figure 2 for an illustration
of the casting kit.)
electrode connectors (2)
running platform
(supports the running tray)
color-coded leads connect
electrodes in the unit base to
the power supply.
lid assembly
ll the base with 50/50
ethylene glycol/water
Mini submarine electrophoresis
unit function
The Hoefer
™
HE 33 horizontal agarose unit
is intended for rapid electrophoresis of small
quantities of nucleic acids in agarose gels. A
gel is cast in the gel caster, which holds one or
two combs. (Eight different combs are avail-
able; a maximum of 32 samples can be run
if two 16-well combs are used.) After the gel
sets, the running tray is transferred to the plat-
form of the horizontal unit. The base of the
unit holds coolant that can be chilled before
the run. This passive cooling capacity allows
fast, high voltage runs.
•
p2
Important information
• The safety lid must be in place before connecting
the power leads to a power supply.
• Turn all power supply controls off and disconnect
the power leads before removing the safety lid.
• Before the rst use, ll the base with about 600 ml
50/50 ethylene glycol/water to prevent irreparable
damage to the unit. (See instructions on page
4.) The base must be lled even if no cooling is
required.
• Do not use pure water, commercial anti-freeze, or
any organic solvent to ll the base. Water expands
as it freezes. Because the water is trapped in the
base, it may crack. Organic solvents will cause
irreparable chemical damage to the unit!
• Do not chill the base below -20 °C (-4 °F). Chill the
base in a bucket of ice, refrigerator, or in a freezer.
• Do not operate with gel or buffer temperature above
50 °C. Prevent overheating by chilling the base prior
to use. To prevent overheating during prolonged
high-voltage runs, exchange the rst base with an
extra prechilled second base if one is available.
Overheating will cause irreparable damage to the
unit!
• If this equipment is used in a manner not speci ed
by the manufacturer, the protection provided by the
equipment may be impaired.
• Only accessories and parts approved or supplied by
Hoefer, Inc. may be used for operating, maintaining,
and servicing this product.
•
p3
Informations importantes
• Le couvercle de sécurité doit être en place avant
de brancher les prises au générateur.
• Eteindre le générateur et débrancher les prises
avant d’enlever le couvercle de sécurité.
• Avant la premiére utilisation, remplir le socle
avec environ 600 ml 50/50 d’éthylène glycol et
d’eau a n de ne pas endommager l’instrument.
(Voir page 4 du mode d’emploi). Le socle doit
être rempli même si le refroidissement n’est pas
nécessaire.
• Ne pas utiliser seulement de l’eau, de l’anti-
gel commercial ou tout autre solvant organique
pour remplir le socle. L’eau augmente en volume
lorsqu’elle gêle. Du fait que l’eau est enfermée,
le socle peut se fendre. Les solvants organiques
peuvent causer des dommages chimiques
irréparables.
• Ne pas refroidir le socle en dessous de -20 °C
(-4 °F). Refroidir le socle dans un seau rempli de
glace ou un dans un réfrigérateur.
• Ne pas utiliser avec un gel ou un tampon à
plus de 50 °C. Eviter le surchauffement en
refroidissant le socle avant l’emploi. A n d’éviter
le surchauffement durant de longues utilisations
sous haut voltage, échanger si possible, le
socle avec un second socle déjà refroidi.Un
surchauffement peut causer des dommages
irréparables à l’instrument.
• Si l’instrument n’est pas utilisé en conformité
avec les recommandations du fabriquant, les
protections de sécurité qui équipent cet appareil
peuvent être rendues inéf caces.
• Seulement les accessoires et piéces detachées
approuvés ou fournis par Hoefer, Inc. sont
recommandés pour l’utilisation, l’entretien et
réparation de cet appareil.
•
p4
Unpacking
Unwrap all packages carefully and compare
contents with the packing list, making sure all
items arrived. If any part is missing, contact
your local sales of ce. Inspect all components
for damage that may have occurred while the
unit was in transit.
If any part appears damaged,
contact the carrier
immediately. Be sure to keep
all packing material for damage claims or for
repacking should it become necessary to return
the unit.
Speci cations
Max. voltage
500 V for 5 minutes or less
Max. wattage
15 W
Max. current
500 mA
Max. operating temp.
50 °C
Max. buffer volume
250 ml
Coolant required
≈
600 ml
50/50 water/ethylene glycol
Gel size
7
×
10 cm
Environmental
operating conditions
Indoor use: 4–40 °C
Humidity up to 80%
Altitude up to 2000 m
Installation category
II
Pollution degree
2
Dimensions
width
×
depth
×
height
24
×
13
×
7 cm
(9.5
×
5.2
×
2.8 in.)
Weight
(base, lid, leads only)
0.4 kg (0.9 lbs)
Product certi cations
EN61010–1, UL61010A-1,
CSA C22.2 1010.1, CE
Certi ed
This declaration of confor-
mity is only valid for the
instrument when it is:
• used in laboratory locations,
• used as delivered from
Hoefer, Inc. except for
alterations described in the
User Manual, and
• connected to other CE
labeled instruments or
products recommended or
approved by Hoefer, Inc.
•
p5
Operating instructions
Agarose gels are rst prepared using the gel cast-
ing kit. Samples are then loaded into wells and
electrophoretically separated. The uorescent
dye ethidium bromide can be added to the gel or
electrophoresis buffer or both to track separa-
tion progress. After electrophoresis, the gel may
be stained and photographed, blotted, or dried
for autoradiography.
Fill the base with coolant
Even if no cooling is required, it is important to ll
the base with the proper coolant solution before the
rst use because the solution provides a necessary
heat sink.
Prepare 600 ml of 50/50 ethylene glycol/water.
Optional:
To help see wells more clearly while loading
the sample, add a drop or two of soluble dye or food
color to the coolant solution.
Locate the two inlet holes in the top edge of the base.
Fill the base cavity as full as possible with coolant
using a 50-ml syringe or a pump.
Push a gray rubber plug into each hole, taking care
that the plug is securely seated.
Place the prepared base in an ice bucket or into a
refrigerator or freezer set no lower than -20 °C for
about an hour before use. (The base will always be
ready if you store it in the refrigerator or freezer.)
Important!
Do not ll the base
with commercial antifreeze,
organic solvents, or pure
water.
Note:
It is not necessary to
replace the coolant.
•
p6
Prepare solutions
Prepare 250 ml of running buffer. (Refer to p. 12 for
recipes of commonly used electrophoretic running
buffers.)
Prepare the sample loading buffer. (Refer to p. 13 for
a recipe and a table of volume requirements for each
comb size.)
Prepare approximately 7 ml agarose solution per mm
of gel thickness. (For example, a 3-mm gel requires
0.3 cm
×
7 cm
×
10 cm = 21 ml)
Dissolve agarose in running buffer, heat according
to instructions accompanying the agarose, and allow
the solution to cool to 50 °C before pouring into the
casting tray.
Optional:
Add 0.5 µg/ml ethidium bromide to the gel
solution to observe separation during electrophoresis.
Caution:
Ethidium bromide is a
known mutagen. Always wear
gloves when handling.
Fig 2.
Gel casting kit.
Approach the foam pad with one
end of the running tray and then
gently press the tray edge against
the pad, compressing it enough
to allow the opposite end of the
running tray to drop fully into the
casting tray before sealing against
the foam pad.
UV-transparent running tray
(cast the gel on this tray, then
transfer gel to the horizontal unit
base for electrophoresis.)
gel casting tray
foam pads (2)
This manual suits for next models
2
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