Doc. No.: 994-2029 v. 1.1 · Issue date: 03-Apr-2020
ChemoMetec A/S · Gydevang 43 · 3450 Allerod · Denmark · suppor[email protected] · www.chemometec.com
Technical Note No. 2029 Rev. 1.1
NucleoCounter® NC-202™Performance data
The NucleoCounter® NC-202™ is a high precision cell counter using the Via2-Cassette™for sample loading
and staining. The data in this document demonstrates the performance of the NucleoCounter® NC-202™
in comparison with manual cell counting.
Introduction
Cell density greatly impacts cell behavior in a broad
range of cell-based applications such as research
experiments, bioassays, and bioprocessing.
Precise and robust cell counting is critical to achieving
reproducibility in such applications. The following
document summarizes the performance of the
NucleoCounter® NC-202™in comparison with manual
cell counting using a generic counting chamber (the
Bürker-Türk) and trypan blue.
Background
The NucleoCounter® NC-202™ is a high precision cell
counter using low magnification fluorescence
microscopy and automated image analysis to identify
live and dead cells.
The Via2-Cassette™ combines cell sampling, staining,
and loading of the counting chamber into a single
workflow. Together, the NucleoCounter® NC-202™and
Via2-Cassette™ generate data with low inter- and intra-
operator variation. The NucleoCounter® NC-202™ can
count all mammalian cell types, including primary cells
and aggregated cells.
NC-View™, the accompanying NucleoCounter® NC-
202™ software, provides operational control and easy
validation of cell counts by displaying images and
results in an intuitive user interface. NC-View™ is
designed to maintain data integrity and is compatible
with the 21 CFR Part 11 guidelines.
This document summarizes a complete dataset where
a large panel of cell lines were counted with three
NucleoCounter® NC-202™ instruments in parallel with
manual counting.
Conclusion
The NucleoCounter® NC-202™ displays superior
performance to manual counting in terms of linearity,
and precision and has low instrument-instrument
variation.
Experimental setup
A panel of cell types (Appendix I) were counted using three different NucleoCounter® NC-202™
instruments. Cell counts were performed using the standard ‘Count & Viability’ protocol with Via2-
Cassettes™. Manual cell counting was done in parallel, to serve as a counting reference. Manual counts
were carried out in duplicates using 0.4% trypan blue and a Bürker-Türk counting chamber. The same
operator performed all the manual cell counts to minimize counting variation.
