AmScope 340 Series User manual

340

Series

User’s Manual

340

Series

abl

Contents

Before Use..........................................................................................3

Introduction ............................................................................................................. 3

Safety Precautions ................................................................................................... 4

Parts .............................................................................................. 5

B340 ................................................................................................................. 5

T340 ................................................................................................................. 6

Definition of Parts ............................................................................................... 7

Getting Started ............................................................................ 8

Assembly ..................................................................................................................

Operation .................................................................................... 9

Setting Up ................................................................................................................... 9

Focusing ................................................................................................................... 9

Adjusting The Condenser & Diaphragm ......................................................... 10

Using the Trinocular Port ................................................................................. 10

Attaching a Camera ........................................................................................... 10

Setting the Stage’s Stop-Limit .............................................................................. 11

Adjusting Focusing Tension ........................................................................... 11

Changing the Bulb ............................................................................................. 11

Maintenance/Precautions ..................................................................................... 12

Specifications.............................................................................. 13

340 Series Specifications ...................................................................................... 13

Technical Parameters ................................................................ 14

Technical Terms & Concepts .......................................................................... 14

Troubleshooting........................................................................ 15

Common Issues ............................................................................................. 15

Common Issues (Continued) ......................................................................... 16

340

Series

Before

Use

Introduction

Congratulations on the purchase of your new AmScope microscope!

This manual is designed for the 340 series microscopes (B340 & T340).

Please take a few

minutes to familiarize yourself with the features and functions of your new mi- croscope.

If you’d like more information on microscopes, parts, or accessories, please visit our website at:

www.amscope.com

We highly recommend you study this manual thoroughly before operating the microscope and

that you keep it on hand for future reference.

If you have additional questions or need assistance, please send us an email at:

info@amscope.com

340

Series

Before

Use

Safety Precautions

As the microscope is a precision instrument, always handle it with care, avoiding impact

or abrupt movement during transportation. Do not shake the package.

Do not place the microscope in direct sunlight or in high heat. Keap it indoors in a dry

and clean place with temperatures between 32-100 degrees F (0-40 degrees C), and in

maximum relative humidity of 85%.

Avoid touching the lenses on the objectives and the eyepieces so that oil and dirt from

your fingerprints do not obstruct your view.

Before turning the power on, make sure that the power supply voltage is consistent with

the voltage of your microscope.

340

Series

Parts

B340

Binocular Head

Eyepieces

Objectives

MechanicalStage

Coarse Focusing

Knob

Iris Adjustment Slider Fin

sin

Knob

Dry Darkfield Condenser

(with -DK Only)

Mechanical Stage Controls

340

Series

Parts

T340

Trinocular Port

Trinocular Head

Eyepieces

Objectives

MechanicalStage Coarse Focusing Knob

Iris Adjustment Slider

Fine Focusing Knob

Base Lens

Mechanical Stage Controls

340

Series

Parts

Definition of Parts

AdjustableTrinocularPort

Threaded port with adjustable length of attachment of digital or video camera

Base Lens

Directs the light source towards the slide

Coarse FocusingKnob

Used to initially bring the slide into the sight and focus

Condenser &IrisDiaphragm

Controls the amount of light that hits the slide

Condenser Screw

Screws the condenser in place, securing it to the mechanical stage

Dimmer

Controls the amount of light that escapes from the base lens

Diopter

Allows the focus to be perfected for both eyes, independent of each other

Fine Focusing Knob

Used to refine focus to clear image

Head Lock Screw

Screws the microscope head into place

Limit Stop Knob

Limits the upward movement of the mechanical stage in order to avoid damaging the slide and objective

Mechanical Stage

Mechanically moves the slide along an X and Y axis for optimal positioning

Nosepiece

Houses the objective lenses

Rotating Head

Adjusts eyepieces to fit the distance between the user’s eyes for added comfort

Tension Knob

Adjusts the tension of the focusing knobs

340

Series

Getting

Started

Assembly

First, take the styrofoam container out of the cardboard carton and lay it on its side, paying attention to

which side is labeled up. Remove the tape and open the container carefully so as to avoid dropping and

damaging the optical items. Check carefully to ensure that all parts and accessories are intact.

Check the packing list to ensure that you’re received all items:

One microscope body

One binocular (B340) or one trinocular (T340) head

One trinocular photo port (for T340 only)

Four objective lenses: 4X, 10X, 40X, 100X

Two 10Xeyepieces (additional eyepieces included with certain models)

One tension-adjustment spanner

Blue color filter (for LED models only)

Blue, green, yellow color filters (halogen models only)

One bottle of immersion oil

One spare fuse (halogen models only)

One spare 6V/15W bulb (halogen models only)

One dust cover

Note: If you have an LED model, there is no need to change light bulb on it. If the light is not working, please ensure

that the unit is plugged in and the power is on. If still not working, contact us, as there may be another cause. The

LED bulb is designed to far outlast the other components on the unit.

Remove the microscope body from the box and remove the plastic protective covering. The body of the

micro-

scope is composed of the base, the stage, the arm, and the nosepiece.

Loosen the metal knob located directly above the nosepiece (the head-lock screw) completely. Place the head

(flat, circular side down) into the circular opening on top of the arm. Tighten the head-lock screw to secure the

head in place.

For the trinocular model, screw the photo port onto the top of the microscope head (trinocular C-mount

port).

Remove the ocular-tube dust-caps and slide the desired eyepieces into the ocular tubes. Be sure to avoid

touching the lens to ensure no artifacts appear in your image.

Screw the objectives into the microscope nosepiece from the lowest magnification to the highest, again avoid-

ing touching the lenses.

Plug in the microscope and turn it on.

340

Series

Operation

Setting Up

Loosen the head lock screw and adjust the microscope head so that it is in a comfortable position for observa-

tion. Lock the head-lock screw.

With both eyes open, look into the eyepieces. Adjust the interpupillary distance by holding the eyepiece tubes

and rotating the eyepiece tubes either towards or away from each other until only one circle of light is seen by both

eyes.

Place the specimen to be studied on a glass slide (or use a prepared slide). Place it on the stage, holding it snugly

in place with the metal slide holders (clips) of the mechanical stage.

Using the mechanical stage’s slide controls, center the specimen over the stage opening, lining it up with the light

and the objective lens.

To adjust the illumination, slowly turn the dimmer knob on the right side of the body until the desired intensity

of light is achieved. You may also adjust the iris on the light source itself by rotating the ring around the base lens

or the iris on the condenser, so please make sure all are adjusted to the correct positions.

Focusing

Turn the nosepiece to choose an objective. It is easiest to use the lowest magnification first (4x objective) to lo-

cate and focus on the specimen. As you move up in magnification you may need to refocus the image a little each

time.

When using the 100x objective, a drop of immersion oil should be placed between the cover slip and the objec-

tive to minimize distortion caused by air. Always be sure to wipe the oil off of the objective with either lens cleaning

paper or a nonabrasive, lint free cloth.

Being focusing by first looking with one eye through the eyepiece without the diopter. Close your other eye. Use

the coarse focusing knob to adjust the height of the stage until the sample comes into clear focus.

Note: You may loosen the limit-stop screw (located rear of the mechanical stage) in order to give yourself a larger-

range of motion for fine tuning the focus.

Once the image is clear in your field of view, you will want to use the fine focusing knob to tune it for best results.

Note: Please be careful when moving the mechanical stage if you need to re-center the sample, or if moving the

stage very close to the objectives. The limit stop is designed to prevent impact between objective and slide, so when

it is off you will be able to damage the microscope. For safety, when using the 40x and 100x objectives, engage the

limit stop once you have it close or in contact with the objective (if using oil contact is required).

340

Series

Operation

Adjusting the Condenser & Diaphragm

Using the condenser-adjustment knob, you can change the distance between the light condenser and the stage.

This allows you to control the concentration of the light hitting your slide.

By changing the aperture (hole size) of the iris diaphragm, you can adjust the background brightness. Adjust the

aperture of the iris diaphragm using the iris adjustment slider located directly under the stage.

If you want to use a color filter, swivel the filter holder out, towards you. You can now place the desired color

filter into the circular opening. Slide the filter holder back to original position before observation.

Note: The filter holder is placed in from the factory in a manner in which it swings out and hits the arm of the

microscope (backwards). If this happens, simply grip the condenser assembly and rotate it. It may take a small

amount of force to rotate it, but after doing so, you will be able to swing the filter holder out towards the front of

the unit for easier operation.

Using the Trinocular Port

The AmScope T340 viewing head uses a simul-focal design which allows you to use both eyepieces and the

trinocular photo port simultaneously. The photo port uses a 23mm tube for use with compensating (reduction)

lenses. C-Mount cameras must be equipped with a C-Mount to 23mm compensating lens. With the lens attached

to the camera, simply slide the 23mm lens into the photo port. If the camera's image is not parfocal with the

eyepieces, the 23mm metallic tube on the top of the trinocular port can be adjusted by rotating clockwise or

counter-clockwise to vary the focal length.

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