BioChain AnaPrep 12 Dx User manual

BioChain Institute Inc.
Automated Nucleic Acid Preparation System
Catalog No.: Z2111001
Rev. Date: Jan 15, 2021
Manual No. QW-IH01-06
Version: Z2111001UA
For General Laboratory Use Only
Instruction Manual

F-753-3UMRevA Z2111001UA Active Date: 01152021
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TABLE OF CONTENTS
Page #
Introduction....................................................................................................... 3
Safety Information............................................................................................. 4
1.0 Installation ................................................................................................... 8
1.1 Composition of the AnaPrep 12 Dx system ................................................... 8
1.2 Contents of Reagent Kits............................................................................... 9
1.3 Operating Environment / Condition............................................................ 10
1.4 Unpack the AnaPrep 12 Dx system.............................................................. 12
1.5 Overall View................................................................................................ 13
2.0 Getting Started............................................................................................14
2.1 Turning ON the power ................................................................................ 15
2.2 Touch Panel and User Interface .................................................................. 17
2.3 Preparation................................................................................................. 17
2.4 Function Test .............................................................................................. 17
2.5 Extraction.................................................................................................... 19
3.0 Cleaning & Maintaining the AnaPrep 12 Dx .................................................24
4.0 Troubleshooting ..........................................................................................27
5.0 Specifications ..............................................................................................29
6.0 Warranty.....................................................................................................30

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Introduction
Thank you for purchasing the AnaPrep 12 Dx system which is a fully automated, standalone
robot that can purify nucleic acids within 30-45 minutes. With advanced magnetic bead
separation technology, it enables you to obtain high-quality extraction results. Moreover, the
most user-friendly interface makes users free from troublesome parameter settings and
maintenance.
This guide contains important information regarding the safe use of the AnaPrep 12 Dx system.
Please read this manual carefully before you start to run the system for the first time, especially
for Safety Information.
If there is any question about how to install or operate the system, please contact our technical
Contact Info:
Company: BioChain Institute Inc.
Address: 39600 Eureka Dr.
Newark, CA 94560, USA
Tel: 1-510-783-8588
Fax: 1-510-783-5386
Mail: inf[email protected]
AnaPrep Magnetic Bead Purification Process

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Safety Information
The meaning of safety precaution marks are as follows:
WARNING:
“WARNING”indicates a dangerous condition that may lead to death or serious injury.
BIOHAZARD:
This symbol indicates that certain precautions must be taken when working with
potentially infectious material.
CAUTION:
This symbol indicates that non-compliance with instructions or procedures may lead to
physical injury or even death or could cause damage to the instrument.
Important:
“Important”points an important note for appropriate usage, as well as prohibited actions.
Note:
“Note”indicates the procedures that should be obeyed and supplementary information
for use.
HOT SURFACE:
This symbol labels potentially hot surfaces on the instrument.

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About Instrument
WARNING:
◼Ignoring the following notations may lead to fire or electric shock.
- In countries other than Taiwan, US and Canada, use a power cable that meets your
country’s standard or contact your local distributor.
- Do not use the AnaPrep 12 Dx system with the voltage other than the voltage specified
on the device.
- Do not use the AnaPrep 12 Dx system with a damaged power plug or a loose socket.
- If there is dust on the prongs of the power plug or on the plug socket, remove it with a
dry cloth.
- When you disconnect the plug from the outlet, be sure to hold the power plug itself.
Do not pull the power cable.
- For maintenance, disconnect the power plug from the outlet.
- Do not touch the power plug when you hear the crash of thunder.
◼Do not pour any liquid on the AnaPrep 12 Dx system.
◼Do not place any objects containing liquid on the AnaPrep 12 Dx system. Doing so may
cause device failure, fire, or electric shock.
◼If the device starts to smoke or smells strange, immediately unplug the power cable.
CAUTION:
◼Never attempt to remodel the AnaPrep 12 Dx system without the manufacturer’s
permission. Doing so may cause fire or electric shock.
◼Do not place or drop objects on the AnaPrep 12 Dx system. Also refrain from bumping
or knocking it, as doing so may cause a failure or malfunction of the AnaPrep 12 Dx
system.
◼If any liquid materials are left inside the device, wipe it up with a soft paper tissue, etc.

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Otherwise, the AnaPrep 12 Dx system may be damaged.
◼Repairs to the AnaPrep 12 Dx system should only be performed by such agencies as are
specifically authorized by the BioChain Institute Inc.
◼Only original AnaPrep 12 Dx replacement parts should be used.
If the equipment is used in a manner not specified by the manufacturer, the protection
provided by the equipment may be impaired.
About Reagent Kits
CAUTION:
◼When handling any of the kits, refer to the respective handbook.
◼Reagents in each kit should be handled by observing the safety information and
precautions regarding the kit.
◼Extraction should be performed in an appropriate laboratory or workplace.
Note:
➢The kits are not supplied with the AnaPrep 12 Dx system. Select the desired kit(s) and
order it (them) separately.

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About Samples
BIOHAZARD:
◼Always wear appropriate gloves, a mask, and safety goggles, etc. when handling any
infectious samples.
About Infectious Wastes
◼When handling or disposing of infectious materials, follow the laboratory guideline or
the law regarding infectious waste to perform proper incineration, fusion, sterilization,
and/or disinfection.
◼When you use a third-party company to dispose of wastes, outsource this work to an
operator licensed to handle medical waste subject to special control, and give them the
medical waste manifest at the same time.
BioChain Service Center
◼For technical problem and instrument maintenance please contact our service center:
BioChain Institute Inc.
39600 Eureka Dr.
Newark, CA 94560, USA
1-510-783-8588
1-510-783-5386
tech[email protected]om

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1.0 Installation
1.1 Composition of the AnaPrep 12 Dx system
Check that the following items are included in the package. Contact your local representative if
you notice any missing part(s).
Caution: Please note that the sample rack should only be used with this device.
Caution:
“TURN OFF POWER BEFORE PICKING UP AND INSERTING SD CARD”
To insert the Un-locked SD card in right direction
Please make sure all the components are free from damages as
soon as you get the system. If any damage is found, please contact your local representative
for instant support.
A. AnaPrep 12 Dx X 1
B. Power Cord X 1
C. Sample Rack X 1 (1.5ml)*
D. Barcode Reader X 1
E. SD card
(16GB X 1+32GB X 1 or above)
E. User Manual X 1
G. Fuse X 3 (1A,2A,4A)

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Note:
➢BioChain’s global warranty does not cover damages from transportation or improper
operation.
1.2 Contents of Reagent Kits
A
B
C/D/E/F
G
H
A. Reagent Cartridge(s)
B. Reaction Chamber(s)
C. Tip Holder
D. Piercing pin
E. Small tip
F. Filter tip
G. Sample tube
H. Elution tube
Note:
➢Reagent kits are to be purchased separately. Please contact your local agents or
representatives to get further info.
➢The contents of reagent kits will vary. Refer to the handbook of kits enclosed in
reagent box for details.

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1.3 Operating Environment / Condition
Use the AnaPrep 12 Dx system in a location that meets the following conditions:
◼The AnaPrep 12 Dx system must be placed at a minimum distance of 5-10 cm
from all sidewalls to allow for good air circulation. The space for the allocation of
the AnaPrep 12 Dx system must remain 5-10 cm from instrument to sidewalls.
◼A location where power supply is provided safely.
◼A location where the temperature ranges from 15 to 30°C and humidity ranges
from 30% to 80% RH. (non-condensing)
◼A location that is flat and stable, with no vibration.
◼A location away from direct sunlight. (Block the sunlight by closing curtains or
blinds as necessary)
◼A location which is well-ventilated and not dusty.
◼A location where the temperature does not go up and down suddenly. (Warming
a cold room suddenly or moving the AnaPrep 12 Dx system from a room with
low temperature to a warm room may cause condensation inside the device,
resulting in abnormal extraction)
◼A location where the temperature and humidity are kept within the specified
range (far from water taps, water heaters, humidifiers, air-conditioners, and
heaters)
◼A location far from objects that generate strong magnetic fields. (motors,
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